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Effects of different doses of glucose and fructose on central metabolic pathways and intercellular wireless communication networks in humans

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food Science and Human Wellness 2024年第4期13卷 1906-1916页

作者： Dingqiang Lu Yujiao Liu Miao Zhao Shuai Yuan Danyang Liu Xinqian Wang Yixuan Liu Yifei Zhang Ming Li Yufeng Lü Guangchang Pang Ruijuan Ren College of biotechnology&food Science Tianjin University of CommerceTianjin Key Laboratory of Food BiotechnologyTianjin 300314China Tianjin Institute for food Safety Inspection Technology Tianjin 300134China

Fructose and glucose are often widely used in food processing and may contribute to many metabolic *** observe the effects of different doses of glucose and fructose on human metabolism and cellular communication,volunteers were given low,medium,and high doses of glucose and *** cytokines,glucose,lactate,nicotinamide adenine dinucleotide(NADH)and metabolic enzymes were assayed,and central carbon metabolic pathway networks and cytokine communication networks were *** results showed that the glucose and fructose groups basically maintained the trend of decreasing catabolism and increasing anabolism with increasing *** with glucose,low-dose fructose decreased catabolism and increased anabolism,significantly enhanced the expression of the inflammatory cytokine interferon-γ(IFN-γ),macrophage-derived chemokine(MDC),induced protein-10(IP-10),and eotaxin,and significantly reduced the activity of isocitrate dehydrogenase(ICDH)and pyruvate dehydrogenase complexes(PDHC).Both medium and high doses of fructose increase catabolism and anabolism,and there are more cytokines and enzymes with significant ***,multiple cytokines and enzymes show strong relevance to metabolic regulation by altering the transcription and expression of enzymes in central carbon metabolic ***,excessive intake of fructose should be reduced to avoid excessive inflammatory responses,allergic reactions and autoimmune diseases.

关键词： Fructose Glucose Central carbon metabolic pathway Metabolic enzymes Cytokine network

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Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses

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Synthetic and Systems biotechnology 2024年第3期9卷 470-480页

作者： Kexin Ren Qiang Wang Jianghua Chen Hengwei Zhang Zhoule Guo Meijuan Xu Zhiming Rao Xian Zhang Key laboratory of Industrial biotechnology of the Ministry of Education School of BiotechnologyJiangnan UniversityWuxiJiangsu214122China Yixing Institute of food and biotechnology Co. LtdYixing214200China

Microbial cell factories utilize renewable raw materials for industrial chemical production,providing a promising path for sustainable *** subtilis is widely used in industry for its food safety properties,but challenges remain in the limitations of microbial *** study proposes a novel strategy based on lifespan engineering to design robust *** chassis cells to supplement traditional metabolic modification strategies that can alleviate cell autolysis,tolerate toxic substrates,and get a higher mass transfer *** modified chassis cells could produce high levels of L-glutaminase,and tolerate hydroquinone to produceα-arbutin *** a 5 L bioreactor,the L-glutaminase enzyme activity of the final strain CRE15TG was increased to 2817.4±21.7 U m L^(-1),about 1.98-fold compared with that of the wild ***α-arbutin yield of strain CRE15A was increased to 134.7 g L^(-1),about 1.34-fold compared with that of the *** our knowledge,both of the products in this study performed the highest yields reported so *** chassis modification strategy described in this study can Improve the utilization efficiency of chassis cells,mitigate the possible adverse effects caused by excessive metabolic modification of engineered strains,and provide a new idea for the future design of microbial cell factories.

关键词： Bacillus subtilis Chassis cell Lifespan engineering Robustness Industrial production

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Structure， properties， bioactivities and structure-activity relationships of polysaccharides from Nostoc flagelliforme grown under three different culture conditions

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food Science and Human Wellness 2024年

作者： Yanru Li Shigang Shen Longfei Wang Mengyuan Li Yan Zhang Shiru Jia Ruiying Zhu Peipei Han State Key laboratory of food Nutrition and Safety， Key laboratory of Industrial Fermentation microbiology， Ministry of Education， College of biotechnology， Tianjin University of Science and Technology

The physicochemical properties， structural characteristics， antioxidant， radioprotective and lipid-lowering activities， as well as the underlying structure-activity relationships of polysaccharides extracted from Nostoc flagelliforme grown under normal （WL-EPS-1）， salt stress （NaCl-EPS-1） and mixotrophic culture conditions （Glu-EPS-1） were studied. The results demonstrated that WL-EPS-1， NaCl-EPS-1 and Glu-EPS-1 were heteropolysaccharides comprising different proportions of monosaccharides and uronic acid， with different average molecular weights of 0.99 × 103， 1.09 × 103and 1.18 × 103kDa， respectively. Their intrinsic viscosity values were significantly different， at 24.72 dL/g， 29.98 dL/g and 41.06 dL/g， respectively. The functional groups of polysaccharides were not greatly affected， but the chemical composition， triple-helix structure， chain length and surface morphology were significantly influenced by culture conditions. In vitro bioactivity assays showed that the antioxidant activity generally increased in the order of WL-EPS-1 < NaCl-EPS-1 < Glu-EPS-1， while NaCl-EPS-1 had the best radioprotective effect， and Glu-EPS-1 had the best lipid-lowering effect. In addition， the structure-activity relationship of the polysaccharides was analyzed by Partial Least Squares （PLS）， which revealed that the most important factors affecting the antioxidant， radioprotective and lipid-lowering activities of polysaccharides were viscosity and molecular weight. This study provides a strategy for obtaining high-bioactivity polysaccharides by appropriate regulation of culture conditions， as well as opening new directions for the molecular modification of polysaccharides.

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Characteristics and mechanism of Ni^(2+)and Cd^(2+)adsorption by recovered perlite from agar extraction residue

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Chinese Journal of Chemical Engineering 2024年第8期72卷 141-152页

作者： Juan Wang Yanbing Zhu Zedong Jiang Xiping Du Mingjing Zheng Lijun Li Hui Ni Yuanpeng Wang Zhipeng Li Qingbiao Li College of Ocean food and Biological Engineering Jimei UniversityXiamen 361021China Fujian Provincial Key laboratory of food microbiology and Enzyme Engineering Xiamen 361021China Research Center of food biotechnology of Xiamen City Xiamen 361021China Xiamen Ocean Vocational College Xiamen 361021China College of Chemistry and Chemical Engineering Xiamen UniversityXiamen 361021China

Ni^(2+)and Cd^(2+)in wastewater accumulated through the ecological chain and could jeopardize human *** of Ni^(2+)and Cd^(2+)from wastewater using recovered perlite was an important way to solve the problem of resource utilization of solid waste from agar *** previous study confirmed that recovered perlite from agar extraction residue had better pore size and specific surface area than commercial ***,the adsorption efficiency and adsorption mechanism of recovered perlite were the main factors limiting its adsorption *** adsorption process of Ni^(2+)and Cd^(2+)by recovered perlite in aqueous solution was described by the pseudo-second-order kinetic equation,and the relevant adsorption mechanism was mainly *** with commercial perlite,the adsorption removal rate of Ni^(2+)and Cd^(2+)by enzymatic recovered perlite could reach 92.9%and 89.2%,respectively,and were improved by 12.63%and 13.03%.Langmuir isothermal adsorption model could better describe the isothermal adsorption process of recovered perlite on heavy metal Ni^(2+)and Cd^(2+),and the relevant adsorption mechanism was mainly monolayer *** X-ray photoelectron spectroscopy(XPS)results indicated that the decrease of Si—O Si^(2+)hydroxyl coordination bond and the increase of C—Si bond might make the binding effect of recovered perlite with heavy metals *** competitive adsorption of Ni^(2+)and Cd^(2+)by recovered perlite was still dominated by chemisorption and monolayer *** study was expected to provide a theoretical basis and technical support for the removal of Ni^(2+)and Cd^(2+)from wastewater using recovered perlite from seaweed residue.

关键词： Agar extraction residue Recovered perlite Heavy metal ion Adsorption property

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Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris

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Synthetic and Systems biotechnology 2024年第1期9卷 26-32页

作者： Xueqin Lv Yuting Zhang Lingrui Wang Shixiu Cui Yanfeng Liu Jianghua Li Guocheng Du Long Liu Key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationJiangnan UniversityWuxi 214122China Science Center for Future foods Jiangnan UniversityWuxi 214122China Yixing Institute of food biotechnology Co. LtdYixing 214200China Jiaxing Institute of Future food Jiaxing314015China food laboratory of Zhongyuan Jiangnan UniversityWuxi 214122China

Lactoferricin,a multifunctional peptide located in the N-terminal region of lactoferrin,has a broad-spectrum bacteriostatic *** is a promising candidate as a food additive and immune fortification agent and does not have the risks associated with drug residues and drug ***,we performed promoter and host cell screening to achieve the recombinant expression of lactoferricin in Pichia pastoris,showing an initial titer of 19.5 mg/L in *** X-33 using PAOX1 ***,we constructed a 0030-α hybrid signal peptide by fusing the 0030 signal peptide with the pro-sequence of α-factor secretory signal *** further increased the production of lactoferricin,with a titer of 28.8 mg/L in the fermentation supernatant in the shaking ***,we increased the expression of lactoferricin by fusing it with anionic antioxidant *** neutralization of positive charges yielded a titer of 55.3 mg/L in the shaking flask,and a highest titer of 193.9 mg/L in a 3-L *** antimicrobial activity analysis showed that recombinant-expressed lactoferricin exhibited potent antibacterial activity against Escherichia coli,Bacillus subtilis,and Staphylococcus *** study provides a reference for the construction of microbial cell factories capable of efficiently synthesizing antimicrobial peptides.

关键词： Antimicrobial peptide Lactoferricin Recombinant expression Pichia pastoris

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Enhanced extracellular production of alpha-lactalbumin from Bacillus subtilis through signal peptide and promoter screening

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food Science and Human Wellness 2024年第4期13卷 2310-2316页

作者： Yuqi Zhu Pengdong Sun Chunjian Li Yu Zhang Yu Wang Jingyuan Li Yanfeng Liu Jian Chen Yang Deng College of food Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Key laboratory of Special food Processing(Co-construction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Qingdao Special food Research Institute Qingdao 266109China Science Center for Future foods Engineering Research Center of Ministry of Education on Food Synthetic BiotechnologyJiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of *** this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selected as the original strain for the production ofα-*** was found thatα-LA was identified in the pellet after ultrasonic disruption and centrifugation instead of in the fermentation *** original strain most likely only producedα-LA intracellular,but not *** improve the expression and secretion ofα-LA in RIK1285,a library of 173 homologous SPs from the *** 168 genome was fused with target LALBA gene in the pBE-S vector and expressed extracellularly in *** YjcN was determined to be the best signal *** in supernatant were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and purified by nickel column to calculate the highest yield signal *** addition,different promoters(P_(aprE),P_(43),and P_(glv))were compared and *** results indicated that the strain RIK1285-pBE-P_(glv)-YjcN-LALBA had the highestα-LA yield,reaching 122.04μg/*** study demonstrates successful expression and secretion of humanα-LA in *** and establishes a foundation for simulating breast milk for infant formulas and developing bioengineered milk.

关键词： Bacillus subtilis Alpha-lactalbumin Bioengineering milk Signal peptide Promoter screening

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Dynamics design of a non-natural transcription factor responding to androst-4-ene-3,17-dione

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Synthetic and Systems biotechnology 2024年第3期9卷 436-444页

作者： Ming Zhao Mengkai Hu Rumeng Han Chao Ye Xiangfei Li Tianwen Wang Yan Liu Zhenglian Xue Kun Liu Anhui Engineering laboratory for Industrial microbiology Molecular Breeding College of Biology and Food EngineeringAnhui Polytechnic UniversityWuhu 241000China

The production of androst-4-ene-3,17-dione(AD)by the steroidal microbial cell factory requires transcription factors(TFs)to participate in metabolic ***,microbial cell factory lacks effective TFs that can respond to AD in its metabolic ***,finding and obtaining natural TFs that specifically respond to AD is a complex and onerous *** this study,we devised an artificial TF that responds to AD,termed AdT,based on structure-guided molecular dynamics(MD)*** to MD analysis of the conformational changes of AdT after binding to AD,an LBD in which the N-and C-termini exhibited convergence tendencies was used as a microswitch to guide the assembly of a DNA-binding domain lexA,a linker(GGGGS)2,and a transcription activation domain B42 into an artificial *** a proof of design,a AD biosensor was designed and constructed in yeast on the basis of the ligand-binding domain(LBD)of hormone *** addition,the transcription factor activity of AdT was increased by 1.44-fold for its variant ***,we created non-natural TF elements for AD microbial cell factory,and expected that the design TF strategy will be applied to running in parallel to the signaling machinery of the host cell.

关键词： Protein design Artificial transcription factor Molecular dynamics Steriods Site-mutations

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Epicatechin attenuates lead(Pb)-induced cognitive impairment in mice:regulation on Nrf2 signaling pathway,and interference on the interaction between Pb with albumin

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food Science and Human Wellness 2024年第2期13卷 1065-1078页

作者： Dai Cheng Qianqian Yu Kexin Zhu Dingdong Bu Zijian Wu State Key laboratory of food Nutrition and Safety Tianjin University of Science&TechnologyTianjin 300457China School of biotechnology and food Science Tianjin University of CommerceTianjin 300134China

Epicatechin(EC)was used in this study to antagonize the cognitive dysfunction caused by lead(Pb)exposure in ***-week-old male Kunming mice were treated with PbCl_(2)(20 mg/kg)and/or EC(50 mg/kg)by gavage administration for 4 *** water maze test showed that EC could improve memory dysfunction induced by *** antagonized Ca^(2+)overload,activated Nrf2 signaling pathway and reduced the accumulation of Pb in the brain and serum,which suggested that EC might alter Pb distribution in *** vitro,spectroscopic analysis,potentiometric titration and docking studies were applied to inquiry into the interaction between bovine serum albumin(BSA)and Pb^(2+)in presence or absence of *** was proved to chelate Pb^(2+)and reduced the interaction between BSA and Pb^(2+).In summary,EC might protect Pb-induced cognitive impairment by activating Nrf2 signaling pathway,and suppressing Pb accumulation via interference on the binding of Pb to albumin.

关键词： Lead Epicatechin Bovine serum albumin Interaction Oxidative stress

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Single carbon metabolism–A new paradigm for microbial bioprocesses?

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Synthetic and Systems biotechnology 2024年第2期9卷 322-329页

作者： Michael Baumschabl Özge Ata Diethard Mattanovich Austrian Centre of Industrial biotechnology(ACIB) Vienna1190Austria University of Natural Resources and Life Sciences Department of BiotechnologyInstitute of Microbiology and Microbial BiotechnologyViennaAustria

Increasing atmospheric carbon dioxide levels,a reduction of arable land area and the dependence of first and second generation biotechnology feedstocks on agricultural products,call for alternative,sustainable feedstock sources for industrial *** direct use of CO_(2)or conversion of CO_(2)into other single carbon(C1)sources have great potential as they might help to reduce carbon emissions and do not compete with agricultural land *** we discuss the microbial use of C1 carbon sources,their potential applications in biotechnology,and challenges towards sustainable C1-based industrial biotechnology *** focus on methanol,formic acid,methane,syngas,and CO_(2)as feedstocks for bioprocesses,their assimilation pathways,current and emerging applications,and limitations of their *** mini-review is intended as a first introduction for researchers who are new to the field of C1 biotechnology.

关键词： Sustainability BioeconomyCarbon dioxide Methanol Formate Methane

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Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch

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Synthetic and Systems biotechnology 2024年第3期9卷 577-585页

作者： Haoyu Guo Rongzhen Tian Yaokang Wu Xueqin Lv Jianghua Li Long Liu a b Guocheng Du Jian Chen Yanfeng Liu Key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China Science Center for Future foods Jiangnan UniversityWuxi214122China Engineering Research Center of Ministry of Education on food Synthetic biotechnology Jiangnan UniversityWuxi214122China Jiangsu Province Engineering Research Center of food Synthetic biotechnology Jiangnan UniversityWuxi214122China

Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microbial cell factories for efficient bioproduction in synthetic *** comparison to plasmid-based multi-copy expression,the utilization of chromosomal multi-copy genes offers increased stability of expression level,diminishes the metabolic burden on host cells,and enhances overall genetic *** this study,we developed the“BacAmp”,a stabilized gene integration expression and copy number amplification system for high-level expression in Bacillus subtilis,which was achieved by employing a combination of repressor and non-natural amino acids(ncAA)-dependent expression system to create a reversible switch to control the key gene recA for homologous *** the reversible switch is turned on,genome editing and gene amplification can be ***,the reversible switch was turned off therefore stabilizing the gene copy *** stabilized gene amplification system marked by green fluorescent protein,achieved a 3-fold increase in gene expression by gene amplification and maintained the average gene copy number at 10 after 110 *** we implemented the gene amplification system for the regulation of N-acetylneuraminic acid(NeuAc)synthesis,the copy number of the critical gene increased to an average of 7.7,which yielded a 1.3-fold NeuAc *** research provides a new avenue for gene expression in synthetic biology and can be applied in metabolic engineering in ***.

关键词： Bacillus subtilis Stabilized gene amplification system Homologous recombination recA

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