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Advances in Bioscience and Biotechnology 2024年第1期15卷 39-51页

作者： Pamphile Thierry Houngbo Natacha Aivodji Rodrigue Akotegnon Alphonse Sezan laboratory of biomembranes and cellular Signaling Department of Animal Physiology University of Abomey-Calavi Cotonou Benin

The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as selenium and certain vitamins. To assess the thyroid disturbances in the mountain population of Benin, a survey was conducted in Natitingou, a mountain town located in the department of Atacora, in the northwest of Benin, on a sample of thirty (30) adults (15 men and 15 women), most of whom are educated. The results of the questionnaire revealed that 43% of the surveyed population acknowledged having knowledge on the mentioned subject and have dietary habits based on the consumption of seafood, and also legumes (20%). The examination of the serum results of the dosage of T3, T4 and TSH hormones revealed cases of thyroid disturbances in the region (36.32% in men and 44.98% in women). The analysis of a comparative table including the “VEDALAB Easy Reader+” and five (05) other readers, presents the performance, reading techniques, principles, advantages and disadvantages of each device. Pending further studies, some recommendations were made at the end of this study to the academic authorities regarding probable cases of dysthyroidism for which additional examinations are required and an awareness for the improvement of dietary habits.

关键词： Thyroid TSH T3 T4 “VEDALAB Easy Reader+”

Evaluation of the Preventive and Therapeutic Activities of <i>Tridax procumbens</i>against Hyperglycemia and Hyperlipidemia Induced in Wistar Rats

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Pharmacology & Pharmacy 2021年第7期12卷 127-140页

作者： Jean-Baptiste Amagbegnon Omédine Koukoui Pascal C. Agbangnan Santorin Seton Mansouratou Betira Sedami Medegan Laura Loko Victorin Dougnon Luc Djogbenou Alphonse Sezan laboratory of Animal Physiology Cellular Signalisation and Pharmacology University of Sciences Technologies Engineering and Mathematics Dassa Zoume Benin laboratory of Study and Research in Applied Chemistry Unity of Research and Molecular Interactions (URIM/LERCA/UAC) Cotonou Benin Medicinal and Organic Chemistry laboratory Faculty of Health Sciences Cotonou Benin laboratory of Applied Entomology University of Sciences Technologies Engineering and Mathematics Dassa Zoume Benin Research Unit in Applied Microbiology and Pharmacology of Natural Substances Research Laboratory for Applied Biology Polytechnic School of Abomey-Calavi University of Abomey-Calavi Abomey-Calavi Benin laboratory of Infectious Vector Borne Diseases Regional Institute of Public Health University of Abomey-Calavi Abomey-Calavi Benin laboratory of biomembranes and cellular signalisation University of Abomey-Calavi Cotonou Benin

From the Asteraceaes family, Tridax procumbens is a wild species frequently found in fields, meadows and roadsides. It is traditionally used to treat yellow fever, typhoid fever, high blood pressure and diabetes. In this work we used the hydro-ethanolic extract of the leaves and stems of the plant and also the fractions of this extract to determine the preventive and therapeutic activities of the plant against obesity, hyperglycemia and hyperlipidemia. The yields of the fractions obtained by decantation of the hydro-ethanolic extract are respectively 0.160% with hexane, 0.073% with dichloromethane, 0.09% with diethyl ether and 0.150% with ethyl acetate. Hyperglycemia and hyperlipidemia were induced in the rats submitted to a fatty and sweet diet for 28 days. Our results showed a significant increase in glycemia, weight and triglyceride level in rats that were subjected to the diet. On the other hand, the rats treated with the dose of 500 mg/kg of body weight of Tridax procumbens extract during the induction showed a reduction in weight gain and an absence of increase in blood sugar and triglyceride levels. The ethyl acetate fraction of Tridax procumbens extract administered to fatty and hyperglycemic rats at 50 mg/kg of body weight for 14 days did not induce change in the blood sugar and triglycerides level of fatty and hyperglycemic rats. If it seems obvious that the extract of Tridax procumbens prevents hyperglycemia and hyperlipidemia, the ethyl acetate fraction does not seem to have a therapeutic effect.

关键词： Tridax procumbens Prevention Treatment Hyperglycemia Hyperlipidemia

Hot-nanoparticle-mediated fusion of selected cells

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Nano Research 2017年第6期10卷 2034-2045页

作者： Azra Bahadori Lene B. Oddershede Poul M. Bendix Blegdamsvej 17 Niels Bohr Institute University of Copenhagen 2100 Copenhagen Denmark Lundbeck Foundation Center for biomembranes in Nanomedicine University of Copenhagen 2100 Copenhagen Denmark

Complete fusion of two selected ceils allows for the creation of novel hybrid cells with inherited genetic properties from both original cells. Alternatively, via fusion of a selected cell with a selected vesicle, chemicals or genes can be directly delivered into the cell of interest, to control cellular reactions or gene expression. Here, we demonstrate how to perform an optically controlled fusion of two selected cells or of one cell and one vesicle. Fusion is mediated by laser irradiating plasmonic gold nanoparticles optically trapped between two cells （or a vesicle and a cell） of interest. This hot-particle-mediated fusion causes total mixing of the two cytoplasms and the two cell membranes resulting in formation of a new hybrid cell with an intact cell membrane and enzymatic activity following fusion. Similarly, fusion between a vesicle and a cell results in delivery of the vesicle cargo to the cytoplasm, and after fusion, the cell shows signs of viability. The method is an implementation of targeted drug delivery at the single-cell level and has a great potential for cellular control and design.

关键词： cell fusion membrane fusion gold nanoparticle plasmonic heating optical trapping

Effect of the Ethanolic Extract of Khaya senegalensis on Some Biochemical Parameters on Rabbit’s in Glucose Overload Condition

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American Journal of Plant Sciences 2015年第1期6卷 240-248页

作者： Essé Agossou Mélanie Ahokpe Justin Behanzin Marcellin Takin Ganiou Yessoufou Loica Zohoun Lamine Baba-Moussa Alphonse Sezan Laboratoire de biomembranes et signalisation Cellulaire Département de Physiologie Animale Université d’Abomey-Calavi Cotonou Benin Laboratoire de Biologie et de Typage moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Université d’Abomey Calavi Cotonou Benin

The effect of the ethanolic extract Khaya senegalensis on some biochemical parameters was tested in rabbits with normal glucose and glucose overload. Phytochemical screening after three batches of 6 rabbits race (TBD) were treated with the extract in oral doses of respectively 10, 25 and 50 mg/kg body weight for 30 days;three other groups receiving respectively these doses of the extract were placed under a priori temporary overload of glucose;a control group received instead distilled water treatment. Blood glucose, triglycerides and total cholesterol were measured by spectrophotometry and weight gain of the animals was performed at the beginning of the experiment and every 2 weeks during the study period. The phytochemical screening revealed the presence of polyphenolic compounds (majority) of saponins of anthracene derivatives and steroids in the extract. The test results indicate that under the dose of 50 mg/kg body weight (BW) there was a significant decrease (p < 0.05) in blood glucose after 24 hours. The extract had no effect on the lipid profile or body weight of rabbits.

关键词： Rabbit Khaya senegalensis Glucose Lipid Profile

Ion transporters: emerging agents for anticancer therapy

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Science China Chemistry 2022年第7期65卷 1265-1278页

作者： Tengfei Yan Xin Zheng Shengda Liu Yingping Zou Junqiu Liu State Key laboratory of Powder Metallurgy College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China College of Material Chemistry and Chemical EngineeringKey Laboratory of Organosilicon Chemistry and Material TechnologyMinistry of EducationKey Laboratory of Organosilicon Material Technology of Zhejiang ProvinceHangzhou Normal UniversityHangzhou 311121China

Natural ion channels are pore-forming proteins that allow ions to pass through biomembranes, playing pivotal roles in almost all facets of cellular physiological functions. Biomimetic ion transporters were initially investigated for the purpose of treating lifethreatening channelopathies. Recent studies have indicated that membrane-active synthetic ionophores possess desirable anticancer bioactivity against diverse cancer cell lines by disturbing intracellular ion homeostasis, triggering oxidative stress, and inducing apoptosis in tumors. Recent progress on ionophore-related antitumor therapeutics is comprehensively summarized in this review, including the molecular design principles, functional mechanisms, and characterization methods. Finally, we conclude this review by discussing the future opportunities and challenges in this field. It is anticipated that this review will provide an existing panoramic sketch and future directions toward the construction of novel ion transporters with simplified preparation procedures, enhanced biocompatibility, and desirable anti-proliferative activities, which may further accelerate their therapeutic applications in clinical treatments.

关键词： ion transporter ion homeostasis apoptosis anticancer treatment

An Update on Abscisic Acid Signaling in Plants and More

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Molecular Plant 2008年第2期1卷 198-217页

作者： Aleksandra Wasilewsk.a Florina Vlad Caroline Sirichandra Yulia Redko Fabien Jammes Christiane Valon Nicolas Frei dit Frey Jeffrey Leung Institut des Sciences du Vegetal Centre National de la Recherche Scientifique UPR 23551 Avenue de la Terrasse Bat. 23 91190 Gif-sur-Yvette France CNRS UPR9073 Institut de Biologie Physico-Chimique 13 rue Pierre et Marie Curie 75005 Paris France Laboratoire signalisation et Regulation Coordonnee du Metabolisme Carbone et Azote Institut de Biotechnologie des Plantes (UMR8618) Universite Paris-Sud F-91405 Orsay Cedex France

The mode of abscisic acid （ABA） action, and its relations to drought adaptive responses in particular, has been a captivating area of plant hormone research for much over a decade. The hormone triggers stomatal closure to limit water loss through transpiration, as well as mobilizes a battery of genes that presumably serve to protect the cells from the ensuing oxidative damage in prolonged stress. The signaling network orchestrating these various responses is, however, highly complex. This review summarizes several significant advances made within the last few years. The biosynthetic pathway of the hormone is now almost completely elucidated, with the latest identification of the ABA4 gene encoding a neoxanthin synthase, which seems essential for de novo ABA biosynthesis during water stress. This leads to the interesting question on how ABA is then delivered to perception sites. In this respect, regulated transport has attracted renewed focus by the unexpected finding of a shoot-to-root translocation of ABA during drought response, and at the cellular level, by the identification of a B-galactosidase that releases biologically active ABA from inactive ABA-glucose ester. Surprising candidate ABA receptors were also identified in the form of the Flowering Time Control Protein A （FCA） and the Chloroplastic Magnesium Protoporphyrin-IX Chelatase H subunit （CHLH） in chloroplast-nucleus communication, both of which have been shown to bind ABA in vitro. On the other hand, the protein（s） corresponding to the physiologically detectable cell-surface ABA receptor（s） is （are） still not known with certainty. Genetic and physiological studies based on the guard cell have reinforced the central importance of reversible phosphorylation in modulating rapid ABA responses. Sucrose Non-Fermenting Related Kinases （SnRK）, Calcium-Dependent Protein Kinases （CDPK）, Protein Phosphatases （PP） of the 2C and 2A classes figure as prominent regulators in this single-cell model. Identifying their d

关键词： Abscisic Acid Signaling Plants and More

Click chemistry-based enrichment strategy for tracing cellular fatty acid metabolism by LC-MS/MS

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Journal of Pharmaceutical Analysis 2023年第10期13卷 1221-1231页

作者： Ru-Jie Yang Jian Zou Jia-Yue Liu Jiang-Kun Dai Jian-Bo Wan State Key laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SAR999078China

Fatty acids(FAs),which were initially recognized as energy sources and essential building blocks of biomembranes,serve as the precursors of important signaling *** FA metabolism is essential to understanding the biochemical activity and role of FAs in physiological and pathological *** by the advances in click chemistry for protein enrichment,we herein established a click chemistry-based enrichment(CCBE)strategy for tracing the cellular metabolism of eicosapentaenoic acid(EPA,20:5 n-3)in neural *** alkyne-labeled EPA(EPAA)used as a surrogate was incubated with N2a,mouse neuroblastoma cells,and alkyne-labeled metabolites(ALMs)were selectively captured by an azide-modified resin via a Cu(I)-catalyzed azide-alkyne cycloaddition reaction for *** removing unlabeled metabolites,ALMs containing a triazole moiety were cleaved from solid-phase resins and subjected to liquid chromatography mass spectrometry(LC-MS)*** proposed CCBE strategy is highly selective for capturing and enriching alkyne-labeled metabolites from the complicated *** addition,this method can overcome current detection limits by enhancing MS sensitivity of targets,improving the chromatographic separation of sn-position glycerophospholipid regioisomers,facilitating structural characterization of ALMs by a specific MS/MS fragmentation signature,and providing versatile fluorescence detection of ALMs for cellular *** CCBE strategy might be expanded to trace the metabolism of other FAs,small molecules,or drugs.

关键词： Click chemistry-based enrichment EPA metabolism Terminal alkyne-labeled EPA Azide-modified resin LC-MS/MS

Histological and molecular classification of gliomas

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中国神经肿瘤杂志 2008年第2期6卷 102-102页

作者： Figarella-Branger D Colin C Coulibaly B Quilichini B Maues De Paula A Fernandez C Bouvier C Service d' anatomie pathologique et de neuropathologie hospital de la TimoneMarseilleFrance EA 3281 labo-ratoire de biopathologie de l' adhésion et de la signalisation facultéde médecineuniversitéde la Méditerranée27boulevard Jean-Moulin13385 Marseille cedex 05France.

Rev Neurol (Paris). 2008 June-July;164(6-7):505-515. Epub 2008 Jun *** are the most frequent tumors of the central nervous system. The WHO classification, based on the presumed cell origin,distinguishes astrocytic,oli... 详细信息

关键词：神经胶质瘤临床分析诊断方法肿瘤