检索结果-南通市图书馆

Survey of antioxidant capacity and phenolic composition of blueberry,blackberry,and strawberry in Nanjing

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2012年第2期13卷 94-102页

作者： Wu-yang HUANG Hong-cheng ZHANG Wen-xu LIU Chun-yang LI Department of Functional Food and bioactive compounds Institute of Farm Product ProcessingJiangsu Academy of Agricultural SciencesNanjing 210014China Bee Research Institute Chinese Academy of Agricultural SciencesBeijing 100093China College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China

Berries are a good source of natural antioxidants. In the present study, the total antioxidant capacity and phenolic composition of three berry fruits （blueberry, blackberry, and strawberry） cultivated in Nanjing were investigated. Blueberry, with a Trolox equivalent antioxidant capacity （TEAC） value of 14.98 mmol Trolox/100 g dry weight （DW） exhibited the strongest total antioxidant capacity using both the 2,2-azinobis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt （ABTS） and the 2,2-diphenyl-l-picrylhydrazyl （DPPH） methods. Blueberry also had the highest total phenolic content （TPC, 9.44 mg gallic acid/g DW）, total flavonoid content （TFC, 36.06 mg rutin/g DW）, and total anthocyanidin content （TAC, 24.38 mg catechin/g DW）. A preliminary analysis using high performance liquid chromatography （HPLC） showed that the blueberry, blackberry, and strawberry samples tested contained a range of phenolic acids （including gallic acid, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid, ferulic acid, eUagic acid, and cinnamic acid） and various types of flavonoids （flavone： luteolin; flavonols： rutin, myricetin, quercetrin, and quercetin; flavanols： gallocatechin, epigallocatechin, catechin, and catechin gallate; anthocyanidins： malvidin-3-galactoside, malvidin-3-glucoside, and cyanidin）. In particular, the blueberries had high levels of proanthocyanidins and anthocyanidins, which might be responsible for their strong antioxidant activities. These results indicate a potential market role for berries （especially blueberries） as a functional food ingredient or nutraceutical.

关键词： Berries Antioxidants Phenolics Flavonoids Anthocyanidins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Investigation on cyanobacterial production of the proposed neurotoxinβ-N-methylamino-L-alanine(BMAA)

引用

Water Biology and Security 2023年第4期2卷 128-135页

作者： Zi-Qian Wang Suqin Wang Ju-Yuan Zhang Gui-Ming Lin Nanqin Gan Lirong Song Xiaoli Zeng Cheng-Cai Zhang State Key laboratory of Freshwater Ecology and biotechnology and Key laboratory of algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhan430072PR China University of Chinese Academy of Sciences Beijing100049PR China Institut AMU-WUT Wuhan University of TechnologyWuhanPR China

β-N-methylamino-L-alanine(BMAA)is an environmental neurotoxin thought to be produced by ***,the cyanobacterial origin of BMAA remains *** detection method and culture conditions of cyanobacteria are often cited as factors behind the discrepancy of published *** showed previously that BMAA was highly toxic to the cyanobacterium Nostoc PCC 7120,and it is taken up via an amino acid transport *** a mutantΔnatAΔbgtA deficient in amino acid transport as a genetic control,we show here that BMAA taken up from the medium can be detected quantitatively at a threshold similar to,or below those reported,but was undetectable in the *** BMAA isomer,2,4-diaminobutanoic acids(DAB),but not BMAA,could be detected in cell free extracts of Nostoc PCC ***-term(20 days)diazotrophic growth or nonlimiting supply of phosphate,conditions reported to enhance BMAA synthesis,did not lead to the detection of *** UPLC-MS/MS signal with a similar retention time to BMAA was found after prolonged diazotrophic incubation,but did not have fragment ions of BMAA after further *** extended to 29 different cyanobacterial strains and 6 natural cyanobacterial bloom samples,none of them was found to produce *** cytotoxicity of BMAA to cyanobacteria,and the lack of a cellular protective mechanism against such toxicity,contradict the presence of a BMAA synthesis pathway in these *** specific methods for BMAA detection in vivo need to be developed to clarify the cyanobacterial origin of BMAA.

关键词： Neurodegenerative disease Cyanobacteria BMAA detection algal bloom

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular detection of microbial communities associated with Microcystis vs Synechococcus dominated waters in Tianjin,China

引用

Journal of Oceanology and Limnology 2018年第4期36卷 1145-1156页

作者： HUO Da CHEN Youxin LIU Ping LI Yiming QIAO Zhiyi LI Renhui Department of Fisheries Sciences Tianjin Agricultural UniversityNational Demonstration Center for Experimental Aquaecology and Aquaculture EdueationTianjin 300384China Key laboratory of algal Biology State Key Laboratory of Freshwater Ecology and Biotechnology of ChinaInstitute of HydrobiologyChinese Academy of SciencesWuhan 430072China Department of Basic Sciences Tianjin Agricultural UniversityTianjin 300384China University of Chinese Academy of Sciences Beijing 100049China

Harmful cyanobacterial blooms cause many ecological disasters worldwide. During the development of cyanobacterial blooms, the diversity and domination of cyanobacterial taxa are of a particular concern. In this study, the microbial community structure within a water system, such as in Yuqiao Reservoir and Haihe River in Tianjin City, China, was compared by using next-generation sequencing. A total of 5 001 operational taxonomic units were obtained and clustered from filtered 16 S rDNA V3–V4 region sequences. The cyanobacterial and microbial structures greatly differed in these two water areas. Microcystis was dominant in Yuqiao, whereas Synechococcus was dominant in Haihe. Proteobacteria species were dominant among all detected samples. The relative abundances of Bacteroidetes and Planctomycetes were higher in Yuqiao Reservoir than in Haihe River, whereas Firmicutes and Verrucomicrobia were relatively abundant in Haihe River. Further analyses indicated that the domination of both cyanobacteria was strongly related to several environmental factors, such as total nitrogen, total phosphorus, and dissolved oxygen, reflecting the role of trophic states in shaping the dominance of cyanobacterial taxa. The present study provided the example for Microcystis and Synechococcus dominance along a cyanobacterial bloom in north China. Applying highthroughput sequencing could offer a wide field of vision in analyzing microbial community structures.

关键词： community structure cyanobacteria Yuqiao Reservoir Haihe River dominating patterns

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mechanisms and biotechnological applications of transcription factors

引用

Synthetic and Systems biotechnology 2023年第4期8卷 565-577页

作者： Hehe He Mingfei Yang Siyu Li Gaoyang Zhang Zhongyang Ding Liang Zhang Guiyang Shi Youran Li Key laboratory of Industrial biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxiJiangsu Province 214122PR China National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing Jiangnan University1800 Lihu AvenueWuxiJiangsu Province 214122PR China Jiangsu Provisional Research Center for bioactive Product Processing Technology Jiangnan University1800 Lihu AvenueWuxiJiangsu Province 214122PR China

Transcription factors play an indispensable role in maintaining cellular viability and finely regulating complex internal metabolic *** crucial bioactive functions rely on their ability to respond to effectors and concurrently interact with binding *** advancements have brought innovative insights into the understanding of transcription *** this review,we comprehensively summarize the mechanisms by which transcription factors carry out their functions,along with calculation and experimental-based methods employed in their ***,we highlight recent achievements in the application of transcription factors in various biotechnological fields,including cell engineering,human health,and ***,the current limitations of research and provide prospects for future investigations are *** review will provide enlightening theoretical guidance for transcription factors engineering.

关键词： Transcription factors Binding sites Database-based prediction Experimental identification Cell engineering Biomanufacturing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering Escherichia coli for high-yield production of ectoine

引用

Green Chemical Engineering 2023年第2期4卷 217-223页

作者： Daoan Wang Jiamin Chen Yang Wang Guocheng Du Zhen Kang The Science Center for Future Foods Jiangnan UniversityWuxi214122China The Key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationJiangnan UniversityWuxi214122China The Key laboratory of Industrial biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China

Ectoine is a natural macromolecule protector and synthesized by some *** provides protections against radiation-mediated oxidative damages and is widely used as a bioactive ingredient in pharmaceutics and *** meet its growing commercial demands,we engineered Escherichia coli strains for the high-yield production of *** ectABC gene cluster from the native ectoine producer Halomonas elongata was intro-duced into different Escherichia coli(***)strains via plasmids and 0.8 g L^(-1)of ectoine was produced in flask cultures by engineered *** BL21(DE3).Subsequently,we designed the ribosome-binding sites of the gene cluster to fine-tune the expressions of genes ectA,ectB,and ectC,which increased the ectoine yield to 1.6 g L^(-1).After further combinatorial overexpression of Corynebacterium glutamicum aspartate kinase mutant(G1A,C932T)and the *** aspartate-semialdehyde dehydrogenase to increase the supply of the precursor,the titer of ectoine reached to 5.5 g L^(-1)in flask ***,the engineered strain produced 60.7 g L^(-1)ectoine in fed-batch cultures with a conversion rate of 0.25 g/g glucose.

关键词： Ectoine Escherichia coli Metabolic engineering RBS design

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Role of marine natural products in the development of antiviral agents against SARS‑CoV‑2:potential and prospects

引用

Marine Life Science & Technology 2024年第2期6卷 280-297页

作者： D.P.Nagahawatta N.M.Liyanage Thilina U.Jayawardena H.H.A.C.K.Jayawardhana Seong‑Hun Jeong Hyung‑Jun Kwon You‑Jin Jeon Department of Marine Life Sciences Jeju National UniversityJeju 690-756Republic of Korea Department of Chemistry Biochemistry and PhysicsUniversitédu QuébecàTrois-RivièresTrois-RivièresQC G8Z 4M3Canada Marine Science Institute Jeju National UniversityJeju 63333Republic of Korea Functional Biomaterial Research Center Korea Research Institute of Bioscience and BiotechnologyJeongeup-siRepublic of Korea

A novel coronavirus,known as severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has surfaced and caused global concern owing to its ***-CoV-2 is the causative agent of coronavirus disease 2019;however,it was only discovered at the end of the year and was considered a pandemic by the World Health ***,the develop-ment of novel potent inhibitors against SARS-CoV-2 and future outbreaks is urgently *** naturally occurring bioactive substances have been studied in the clinical setting for diverse *** intricate infection and replication mechanism of SARS-CoV-2 offers diverse therapeutic drug targets for developing antiviral medicines by employing natural products that are safer than synthetic *** natural products(MNPs)have received increased attention in the development of novel drugs owing to their high diversity and ***,this review article investigates the infection and replication mechanisms,including the function of the SARS-CoV-2 genome and ***,we highlighted anti-SARS-CoV-2 therapeutic intervention efforts utilizing MNPs and predicted SARS-CoV-2 inhibitor design.

关键词： SARS-CoV-2 Marine natural products Drug targets Inhibitors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

“Life is short,and art is long”:RNA degradation in cyanobacteria and model bacteria

引用

mLife 2022年第1期1卷 21-39页

作者： Ju-Yuan Zhang Wolfgang R.Hess Cheng-Cai Zhang State Key laboratory of Freshwater Ecology and biotechnology and Key laboratory of algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhanChina Genetics and Experimental Bioinformatics Faculty of BiologyUniversity of FreiburgFreiburgGermany Institut WUT-AMU Aix-Marseille University and Wuhan University of TechnologyWuhanChina

RNA turnover plays critical roles in the regulation of gene expression and allows cells to respond rapidly to environmental *** bacteria,the mechanisms of RNA turnover have been extensively studied in the models Escherichia coli and Bacillus subtilis,but not much is known in other *** are a diverse group of photosynthetic organisms that have great potential for the sustainable production of valuable products using CO_(2)and solar energy.A better understanding of the regulation of RNA decay is important for both basic and applied studies of *** analysis shows that cyanobacteria have more than 10 ribonucleases and related proteins in common with *** and ***,and only a limited number of them have been experimentally *** this review,we summarize the current knowledge about these RNAturnover-related proteins in *** many of them are biochemically similar to their counterparts in *** and ***,they appear to have distinct cellular functions,suggesting a different mechanism of RNA turnover regulation in *** identification of new players involved in the regulation of RNA turnover and the elucidation of their biological functions are among the future challenges in this field.

关键词： cyanobacteria RNA maturation RNA metabolism RNA turnover ribonucleases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enhancement of phycocyanobilin biosynthesis in Escherichia coli by strengthening the supply of precursor and artificially self-assembly complex

引用

Synthetic and Systems biotechnology 2023年第2期8卷 227-234页

作者： Yuqi Wang Ning Li Xiaoyu Shan Xinrui Zhao Yang Sun Jingwen Zhou Key laboratory of Straw Comprehensive Utilization and Black Soil Conservation Ministry of EducationCollege of Life SciencesJilin Agricultural UniversityChangchun130118China Key laboratory of Industrial biotechnology Ministry of Education and School of BiotechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China Science Center for Future Foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Provisional Research Center for bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China

Phycocyanobilin(PCB)is widely used in healthcare,food processing,and *** coli is the common engineered bacterium used to produce ***,it still suffers from low production level,pre-cursor deficiency,and low catalytic *** this study,a highly efficient PCB-producing strain was ***,chassis strains and enzyme sources were screened,and copy numbers were optimized,affording a PCB titer of 9.1 mg/*** importantly,the rate-limiting steps of the PCB biosynthetic pathway were determined,and the supply of precursors necessary for PCB synthesis was increased from endogenous sources,affording a titer of 21.4 mg/***,the key enzymes for PCB synthesis,HO1 and PcyA,were assembled into a multi-enzyme complex using the short peptide tag RIAD-RIDD,and 23.5 mg/L of PCB was ***,the basic con-ditions for PCB fermentation were initially determined in 250 mL shake flasks and a 5-L bioreactor to obtain higher titers of *** final titer of PCB reached 147.0 mg/L,which is the highest reported titer of PCB so *** research provided the foundation for the industrial production of PCB and its derivatives.

关键词： Phycocyanobilin Heme Metabolic engineering Escherichia coli

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Britanin–a beacon of hope against gastrointestinal tumors?

引用

World Journal of Clinical Oncology 2024年第4期15卷 523-530页

作者： Agnieszka Kajdanek Damian Kołat Lin-Yong Zhao Mateusz Kciuk Zbigniew Pasieka Żaneta Kałuzińska-Kołat Department of Biomedicine and Experimental Surgery Medical University of LodzLodz 90-136LodzkiePoland Department of Functional Genomics Medical University of LodzLodz 90-752LodzkiePoland Department of General Surgery&laboratory of Gastric Cancer State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Gastric Cancer Center West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Molecular biotechnology and Genetics University of LodzLodz 90-237LodzkiePoland

Britanin is a bioactive sesquiterpene lactone known for its potent anti-inflammatory and anti-oxidant *** also exhibits significant anti-tumor activity,suppressing tumor growth in vitro and in *** current body of research on Britanin includes thirty papers predominantly related to neoplasms,the majority of which are gastrointestinal tumors that have not been summarized *** drive academic debate,the present paper reviews the available research on Britanin in gastrointestinal *** also outlines novel research directions using data not directly concerned with the digestive system,but which could be adopted in future gastrointestinal *** was found to counteract liver,colorectal,pancreatic,and gastric tumors,by regulating proliferation,apoptosis,autophagy,immune response,migration,and *** confirmed in pancreatic,gastric,and liver cancer,its most commonly noted molecular effects include nuclear factor kappa B and B-cell lymphoma 2 downregulation,as well as Bcl-2-associated X protein ***,it has been found to induce the Akt kinase and Forkhead box O1 axis,activate the AMP-activated protein kinase pathway,elevate interleukin-2 and peroxisome proliferator-activated receptor-γlevels,reduce interleukin-10,as well as downregulate matrix metalloproteinase-9,Twist family bHLH transcription factor 1,and *** also inhibits Myc–HIF1αinteraction and programmed death ligand 1 transcription by interrupting the Ras/RAF/MEK/ERK pathway and mTOR/P70S6K/4EBP1 *** research should aim to unravel the link between Britanin and acetylcholinesterase,mast cells,osteolysis,and ischemia,as compelling data have been provided by studies outside the gastrointestinal *** the cytotoxicity of Britanin on noncancerous cells is significantly lower than that on tumor cells,while still being effective against the latter,further in-depth studies with the use of animal models are *** compound exhi

关键词： Britanin Sesquiterpene lactones Chemotherapeutics Gastrointestinal tumors In vitro In vivo

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Involvement of phosphatidate phosphatase in the biosynthesis of triacylglycerols in Chlamydomonas reinhardtii

引用

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2013年第12期14卷 1121-1131页

作者： Xiao-dong DENG Jia-jia CAI Xiao-wen FEI Key laboratory of Tropical Crop biotechnology Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences School of Science Hainan Medical College

Lipid biosynthesis is essential for eukaryotic cells, but the mechanisms of the process in microalgae remain poorly understood. Phosphatidic acid phosphohydrolase or 3-sn-phosphatidate phosphohydrolase(PAP) catalyzes the dephosphorylation of phosphatidic acid to form diacylglycerols and inorganic orthophosphates. This reaction is integral in the synthesis of triacylglycerols. In this study, the mRNA level of the PAP isoform CrPAP2 in a species of Chlamydomonas was found to increase in nitrogen-free conditions. Silencing of the CrPAP2 gene using RNA interference resulted in the decline of lipid content by 2.4%–17.4%. By contrast, over-expression of the CrPAP2 gene resulted in an increase in lipid content by 7.5%–21.8%. These observations indicate that regulation of the CrPAP2 gene can control the lipid content of the algal cells. In vitro CrPAP2 enzyme activity assay indicated that the cloned CrPAP2 gene exhibited biological activities.

关键词： Phosphatidate phosphohydrolase 2 Triacylglycerol biosynthesis RNAi Chlamydomonas reinhardtii Nitrogen deprivation Over-expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：