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Comprehensive chemical study on different organs of cultivated and wild Sarcandra glabra using ultra-high performance liquid chromatography time-of-flight mass spectrometry(UHPLC-TOF-MS)

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Chinese Journal of Natural Medicines 2021年第5期19卷 391-400页

作者： WANG Cai-Yun LU Jing-Guang CHEN Da-Xin WANG Jing-Rong CHE Kai-Si ZHONG Ming ZHANG Wei JIANG Zhi-Hong State Key laboratory of Quality Research in Chinese Medicines Macao Institute for Applied Research in Medicine and HealthMacao University of Science and TechnologyTaipaMacaoChina Fujian Key laboratory of Integrative Medicine on Geriatric Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou 350122China Guangxi Key laboratory of Traditional Chinese Medicine Quality Standards Guangxi Institute of Chinese Medicine and Pharmaceutical ScienceNanning 530022China

To illuminate the similarities and differences between wild and cultivated Sarcandra glabra(***),we performed a comprehensively study on 26 batches of cultivated *** and 2 batches of wild *** constituents and distribution characteristics of roots,stems and leaves in both wild and cultivated *** were investigated through UHPLC-TOF-MS *** result revealed that there were significant differences between roots,stems and leaves in *** the chemical contents in the root part were less or even absence than those in leaf and stem,which suggested the root organ could be excluded as ***,the chemical contents of stems and leaves in cultivated *** was sightly higher than that of wild ***,cultivated *** may have a high potential for substitution of wild *** without affecting its pharmaceutical *** summary,our study could provide important information to the molecular basis for quality control of ***.

关键词： S.glabra UHPLC-TOF-MS Root stem Leaf

Local sustained release of PD-1 monoclonal antibody and lenvatinib by thermo-sensitive hydrogel for improving tumor immunotherapy

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Chinese Chemical Letters 2023年第8期34卷 241-247页

作者： Lin Zhai Yujie Shi Yi Yan An Lu Xiaoyu Liu Lei Lei Yi Sun Linxia Jiang Xiangyu Wang Honggang Qian Jiancheng Wang Beijing Key laboratory of Molecular Pharmaceutics and New Drug Delivery Systems State Key Laboratory of Natural and Biomimetic DrugsSchool of Pharmaceutical SciencesPeking UniversityBeijing 100191China Department of Pharmaceutical Analysis School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Key laboratory of Carcinogenesis and Translational Research(Ministry of Education) Department of Hepato-Pancreato-Biliary SurgeryPeking University Cancer Hospital&InstituteBeijing 100142China

In clinic,the combination of intravenous pembrolizumab(PD-1 monoclonal antibody)with oral Lenvatinib(LEN)exhibited an enhanced synergistic benefit for cancer ***,the clinical outcomes were always limited by the problems of inconsistent pharmacokinetic profiles of two drugs,lower drug accumulation in tumor and obvious side effects during the combination ***,in situ-forming thermosensitive hydrogels based on PLGA-PEG-PLGA triblock copolymers were prepared for local administration of anti-PD1 and LEN(P&L@Gel)to improve therapeutic efficacy and *** peritumoral or surgical resection site injection,the significant increased concentrations of both drugs in tumor were observed with the local sustained release of P&L@*** comparison with the group of intraperitoneal anti-PD1 plus oral LEN(P-ip&L-po),significantly higher tumor inhibition efficiency on CT26 tumor models could be obtained in P&L@Gel group,even at the dose of one-eighth of the former,same tumorinhibition effects could be *** enhanced antitumor efficacy of P&L@Gel group was probably associated with the 2.2 folds of increased level of CD8+T cells and the polarization of tumor associated macrophage from M2 to M1 along with the increased drug ***,compared with the obvious side effects of P-ip&L-po group,no significant changes of PLT,ALT and UA in blood,as well as IL-1αand IL-1βin mice paws were observed between P&L@Gel group and untreated *** results suggested that local administration of anti-PD1 and LEN with thermosensitive hydrogel could offer a potential strategy for tumors or tumor postoperative adjuvant treatment.

关键词： Lenvatinib PD-1 antibody Thermosensitive hydrogels Topical administration Tumor immunotherapy

A Phytophthora capsici RXLR Effector Targets and Inhibits a Plant,PPlase to Suppress Endoplasmic Reticulum-Mediated Immunity

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Molecular Plant 2018年第8期11卷 1067-1083页

作者： Guangjin Fan Yang Yang Tingting Li Wenqin Lu Yu DU Xiaoyu Qiang Qujiang Wen Weixing Shan State Key laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University Yangling Shaanxi 712100 China State Key laboratory of Crop Stress Biology for Add Areas and College of Agronomy Northwest A&F University Yangling Shaanxi 712100 China State Key laboratory of Crop Stress Biology for Arid Areas and College of Horticulture Northwest A&F University Yangling Shaanxi 712100 China

Phytophthora pathogens secrete a large arsenal of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Phytophthora effectors manipulate host plant cells still remain largely unclear. In this study, we report that PcAvr3a12, a Phytophthera capsici RXLR effector and a member of the Avr3a effector family, suppresses plant immunity by targeting and inhibiting host plant peptidyl-prolyl cis-trans isomerase （PPlase）. Overexpression of PcAvr3a 12 in Arabidopsis thaliana enhanced plant susceptibility to P. capsici. FKBP15-2, an endoplasmic reticulum （ER）-Iocalized protein, was identified as a host target of PcAvr3a12 during early P. capsici infection. Analyses of A. thaliana T-DNA insertion mutant （fkbp15-2）, RNAi, and overexpression lines consistently showed that FKBP15-2 positively regulates plant immunity in response to Phytophthora infection. FKBP15-2 possesses PPlase activity essential for its contribution to immunity but is directly suppressed by PcAvr3a12. Interestingly, we found that FKBP15-2 is involved in ER stress sensing and is required for ER stress-mediated plant immunity. Taken together, these results suggest that P. capsici deploys an RXLR effector, PcAvr3a12, to facilitate infection by targeting and suppressing a novel ER-Iocalized PPlase, FKBP15-2, which is required for ER stress-mediated plant immunity.

关键词： RXLR effector Avr3a FKBP ER stress immunity Phytophthora capsici

The effects of fall fertilization on the growth of Chinese pine and Prince Rupprecht’s larch seedlings

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Journal of Forestry Research 2020年第6期31卷 2163-2169页

作者： Yan Zhu Shan Li Caiyun Wang RKasten Dumroese Guolei Li Qingmei Li Research Institute of Forestry Chinese Academy of ForestryBeijingPeople’s Republic of China Key laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration BeijingPeople’s Republic of China Ministry of Education Key laboratory of Silviculture and Conservation Beijing Forestry UniversityBeijingPeople’s Republic of China Moscow Forestry Sciences laboratory Rocky Mountain Research StationU.S.Department of AgricultureMoscowIDUSA

Nutrient loading in the fall is a practical way to improve seedling quality and has been proven to increase nutrient accumulation,translocation and *** studies have reported on the variation in free amino acids as a result of fall fertilization,especially for diff erent seasonal needle habits(evergreen,deciduous).Therefore,a balanced two-factor factorial design with one fall fertilization treatment(10 mg N/seedling)and Chinese pine(Pinus tabulaeformis Carr.)and Prince Rupprecht’s larch(Larix principis-rupprechtii Mayr.)seedlings was used to examine growth response over one nursery *** changes between fall fertilization,N storage and free amino acids were *** showed that:(1)stem height,diameter and biomass for both species were similar between controls and fall fertilization treatments;(2)compared to controls,fall fertilization increased Chinese pine needle and root N by 17.7%and 36.9%,*** Prince Rupprecht’s larch,fall fertilization resulted in 26.3%and 34.54%more N in stem and roots,respectively,than controls;(3)the three main amino acids in control and fertilization treatments in Prince Rupprecht’s larch seedlings were glutamine,arginine and proline,and in Chinese pine seedlings were glutamine,arginine andγ-amino butyric acid;(4)total amino acid contents were not signifi cantly increased by fall fertilization,but glutamine in Chinese pine and Prince Rupprecht’s larch increased by 64.2%and 35.2%,*** biomass of Prince Rupprecht’s larch had higher proline contents than Chinese pine,which suggests that the stress resistance of the aboveground tissue may be higher for Prince Rupprecht’s *** results indicate that diff erent plant organs with various response are well adapted to nitrogen loading for nutrient storage in evergreen and deciduous conifer seedlings.

关键词： Amino acids Fall fertilization Larix principis-rupprechtii Seedling quality Pinus tabulaeformis

Intracerebroventricularly-administered 1-methyl-4-phenylpyridinium ion and brain-derived neurotrophic factor affect catecholaminergic nerve terminals and neurogenesis in the hippocampus,striatum and substantia nigra

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Neural Regeneration Research 2018年第4期13卷 717-726页

作者： Jun-Fang Chen Man Wang Ying-Han Zhuang Thomas Behnisch The Institutes of Brain Science the State Key Laboratory of Medical Neurobiologyand the Collaborative Innovation Center for Brain ScienceFudan University

Parkinson＇s disease is a progressive neurological disease characterized by the degeneration of dopaminergic neurons in the substantia nigra.A highly similar pattern of neurodegeneration can be induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP） or 1-methyl-4-phenylpyridinium ion（MPP＋）,which cause the death of dopaminergic *** of MPTP or MPP＋ results in Parkinson＇s disease-like symptoms in ***,it remains unclear whether intracerebroventricular MPP＋ administration affects neurogenesis in the substantia nigra and subgranular zone or whether brain-derived neurotrophic factor alters the effects of MPP＋.In this study,MPP＋（100 nmol） was intracerebroventricularly injected into mice to model Parkinson＇s *** 7 days after administration,the number of bromodeoxyuridine（Brd U）-positive cells in the subgranular zone of the hippocampal dentate gyrus increased,indicating enhanced *** contrast,a reduction in Brd U-positive cells was detected in the substantia *** of brain-derived neurotrophic factor（100 ng） 1 day after MPP＋ administration attenuated the effect of MPP＋ in the subgranular zone and the substantia *** findings reveal the complex interaction between neurotrophic factors and neurotoxins in the Parkinsonian model that result in distinct effects on the catecholaminergic system and on neurogenesis in different brain regions.

关键词： nerve regeneration Parkinson's disease MPTP brain-derived neurotrophic factor intracerebroventricular infusion dopaminergic fibers norepinephrine tyrosine hydroxylase hippocampus substantia nigra striatum neurogenesis neural regeneration

Intermediate-Band Solar cells Based on InAs/GaAs Quantum Dots

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Chinese Physics Letters 2011年第3期28卷 218-220页

作者： YANG Xiao-Guang YANG Tao WANG Ke-Fan GU Yong-Xian JI Hai-Ming XU Peng-Fei NI Hai-Qiao NIU Zhi-Chuan WANG Xiao-Dong CHEN Yan-Ling WANG Zhan-Guo Key laboratory of Semiconductor Materials Science Institute of SemiconductorsChinese Academy of SciencesBeijing 100083 The State Key laboratory for Superlattices and Microstructures Institute of SemiconductorsChinese Academy of SciencesBeijing 100083 Engineering Research Center for Semiconductor Integrated Technology Institute of SemiconductorsChinese Academy of SciencesBeijing 100083

We report the fabrication of intermediate-band solar cells(IBSCs)based on quantum dots(QDs),which consists of a standard P-I-N structure with multilayer stacks of InAs/GaAs QDs in the *** with conventional GaAs single-junction solar cells,the IBSCs based on InAs/GaAs QDs show a broader photo-response spectrum(>1330 nm),a higher short-circle current(about 53%increase)and a stronger radiation *** results have important applications for realizing high efficiency solar cells with stronger radiation hardness.

关键词： InAs/GaAs Solar Quantum

Model acupuncture point:Bone marrow-derived stromal stem cells are moved by a weak electromagnetic field

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World Journal of stem cells 2016年第10期8卷 342-354页

作者： Artem N Emelyanov Marina V Borisova Vera V Kiryanova laboratory of High Laser and Magnetic Technology North-Western State Medical University Training laboratory of Physical Chemistry Institute of ChemistrySt. Petersburg State University Department of Physical Therapy and Rehabilitation North-Western State Medical University

AIM To show the existence of a structural formative role of magnetic fields(MFs) with respect to biological objects by using our proposed model of an *** We introduced a magnetised 10-100 μT metal rod(needle) into culture dishes with a negatively charged working surface and observed during 24 h how cells were arranged by MFs and by electrical fields(EFs) when attached. Rat and human bone marrow-derived stromal stem cells(r BMSCs and h BMSCs), human nonadherent mononuclear blood cells, NCTCs and A172 cells, and Escherichia coli(E. coli) were evaluated. The dish containing BMSCs was defined as the model of an acupoint. r BMSCs proliferative activity affected by the needle was investigated. For investigating electromagnetic field structures, we used the gas discharge visualisation(GDV) *** During 24 h of incubation in 50-mm culture dishes, BMSCs or the nonadherent cells accumulated into a central heap in each dish. BMSCs formed a torus(central ring) with an inner diameter of approximately10 mm only upon the introduction of the needle in the centre of the dish. The cells did not show these effects in 35- or 90-mm culture dishes or hydrophobic dishes or rectangular cuvettes. NCTCs and A172 cells showed unstable the effects and only up to two weeks after thawing. Moreover, we observed that the appearance of these effects depended on the season. In winter, BMSCs showed no the effects. GDV experiments revealed that the resonant annular illumination gradually formed from 10 to 18-20 s in polar solutions with and without cell suspension of BMSCs, NCTCs and E. coli when using circular 50-mm dishes, stimulation at 115 V and switching of the electrode poles at 1 kH z. All these data demonstrate the resonant nature of the central ring. Significant influence of MFs on the rB MSC proliferation rate was not *** BMSCs can be moved by MFs when in the presence of a constant EF and MF, when the cells are in the responsive functional state, and whe

关键词： stem cell movement Magnetic targeting Acupuncture Model acupoint Frizzled-related protein Biology resonance Cytoplasm movement Glycocalyx

Ribonucleotide reductase small subunit M2 promotes the proliferation of esophageal squamous cell carcinoma cells via HuR-mediated mRNA stabilization

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Acta Pharmaceutica Sinica B 2024年第10期14卷 4329-4344页

作者： Jing Zhang Qiong Wu Yifei Xie Feng Li Huifang Wei Yanan Jiang Yan Qiao Yinhua Li Yanan Sun Han Huang Mengmeng Ge Dengyun Zhao Zigang Dong Kangdong Liu Pathophysiology Department School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450000China China-US(Henan)Hormel Cancer Institute Zhengzhou 450000China Tianjian laboratory for Advanced Biomedical Sciences Zhengzhou 450052China State Key laboratory of Esophageal Cancer Prevention and Treatment Zhengzhou 450000China Cancer Chemoprevention International Collaboration laboratory Zhengzhou 450000China The First Affiliated Hospital of Zhengzhou University Zhengzhou 450000China

Esophageal squamous cell carcinoma(ESCC),a malignancy of the digestive system,is highly prevalent and the primary cause of cancer-related deaths worldwide due to the lack of early diagnostic biomarkers and effective therapeutic *** ribonucleotide reductase(RNR)expression has been confirmed to be causally linked to *** study demonstrated that ribonucleotide reductase small subunit M2(RRM2)is significantly upregulated in ESCC tissue and that its expression is negatively correlated with clinical ***,HuR promotes RRM2 mRNA stabilization by binding to the adenine/uridine(AU)-rich elements(AREs)within the 3′UTR,resulting in persistent overexpression of ***,bifonazole is identified as an inhibitor of HuR via computational screening and molecular docking *** disrupts HuR-ARE interactions by competitively binding to HuR at F65,R97,I103,and R153 residues,resulting in reduced RRM2 ***,bifonazole exhibited antitumor effects on ESCC patient-derived xenograft(PDX)models by decreasing RRM2 expression and the dNTP *** summary,this study reveals the interaction network among HuR,RRM2,and bifonazole and demonstrated that bifonazole is a potential therapeutic compound for ESCC through inhibition of the HuR/RRM2 axis.

关键词： Esophageal squamous cell carcinoma(ESCC) Bifonazole Ribonucleotide reductase small subunit M2(RRM2) AU-rich elements(AREs) Hu antigen R(HuR) mRNA stability dNTP Cell proliferation

Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm

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Light(Science & Applications) 2018年第1期7卷 279-288页

作者： Yali Bi Chi Yang Yage Chen Shuai Yan Guang Yang Yaozu Wu Guoping Zhang Ping Wang Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and TechnologyWuhanHubei 430074China MoE Key laboratory for Biomedical Photonics Collaborative Innovation Center for Biomedical EngineeringSchool of Engineering SciencesHuazhong University of Science and TechnologyWuhanHubei 430074China MoE Key laboratory of Quark and Lepton Physics and College of Physics Science and Technology Central China Normal UniversityWuhan 430079China

High-resolution optical microscopes that can break 180 nm in spatial resolution set to conventional microscopies are much-needed ***,current optical microscopes have to rely on exogenous fluorescent labels to achieve high resolution in biological ***,we report near-resonance enhanced label-free stimulated Raman scattering(SRS)microscopy with a lateral resolution near 130 nm,in which the high-resolution image contrast originates directly from a low concentration of endogenous biomolecules,with sensitivity gains of approximately 23 ***,by using a 0.3-m-long optical fiber,we developed hyperspectral SRS microscopy based on spectral focusing *** to enhancements in spatial resolution and sensitivity,we demonstrated highresolution imaging of three-dimensional structures in single cells and high-resolution mapping of large-scale intact mouse brain tissues in *** using enhanced high-resolution hyperspectral SRS,we chemically observed sphingomyelin distributed in the myelin sheath that insulates single *** concept opens the door to biomedical imaging with~130 nm resolution.

关键词： resolution resonance approximate