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Hatschek's pit and origin of pituitary gland

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Acta Oceanologica Sinica 2022年第12期41卷 1-6页

作者： Shicui Zhang Xiaohan Ji Institute of Evolution and marine Biodiversity Ocean University of ChinaQingdao 266003China laboratory for marine biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Pituitary gland,or pituitary for short,is characteristic of all *** a“master gland”controlling a multitude of important functions in the body,its evolutionary origin has been an object of investigations of evolutionary biology for two *** morphological,ultrastructural and immunohistochemical studies suggested the homology of the Hatschek’s pit of amphioxus and vertebrate *** genetics study showed that the development of Hatschek’s pit and vertebrate pituitary is both subject to regulation by the common genes such as Pit1,Lhx3 and *** recent studies demonstrated that the Hatschek’s pit is able to secrete growth hormone(GH)-like hormone and thyroid-stimulating hormone(TSH)-like hormone that both play functions similar to vertebrate GH and *** thus think that the emergence of Hatschek’s pit represents one of important events during endocrine network evolution,which laid a foundation for the subsequent formation of a hypothalamic-pituitary system in vertebrates.

关键词： protochordate amphioxus brain pituitary evolution

Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas:segregation and null allele identification for linkage analysis

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Chinese Journal of Oceanology and Limnology 2009年第1期27卷 74-79页

作者：李莉郭希明张国范 Key laboratory of Experimental marine biology Institute of Oceanology Chinese Academy of Sciences Haskin Shellfish Research laboratory Institute of Coastal and Marine Sciences Rutgers University

Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.

关键词： Crassostrea gigas microsatellite null allele genetic mapping

The completed macronuclear genome of a model ciliate Tetrahymena thermophila and its application in genome scrambling and copy number analyses

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Science China(Life Sciences) 2020年第10期63卷 1534-1542页

作者： Yalan Sheng Lili Duan Ting Cheng Yu Qiao Naomi AStover Shan Gao Institute of Evolution&marine Biodiversity Ocean University of ChinaQingdao 266003China laboratory for marine biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266003China Department of biology Bradley UniversityPeoriaIllinois 61625USA

The ciliate Tetrahymena thermophila has been a powerful model system for molecular and cellular ***,some investigations have been limited due to the incomplete closure and sequencing of the macronuclear genome assembly,which for many years has been stalled at 1,158 scaffolds,with large sections of unknown sequences(available in Tetrahymena Genome Database,TGD,http://***/).Here we completed the first chromosome-level Tetrahymena macronuclear genome assembly,with approximately 300×long Single Molecule,Real-Time reads of the wild-type SB210 cells—the reference strain for the initial macronuclear genome sequencing *** 181 chromosomes were capped with two telomeres and gaps were entirely *** completed genome shows significant improvements over the current assembly(TGD 2014)in both chromosome structure and sequence *** majority of previously identified gene models shown in TGD were retained,with the addition of 36 new genes and 883 genes with modified gene *** new genome and annotation were incorporated into *** new genome allows for pursuit in some underexplored areas that were far more challenging previously;two of them,genome scrambling and chromosomal copy number,were investigated in this *** expect that the completed macronuclear genome will facilitate many studies in Tetrahymena biology,as well as multiple lines of research in other eukaryotes.

关键词： Tetrahymena thermophila macronuclear genome 181 chromosomes DNA scrambling copy number

Impacts of increased α-synuclein on clathrin-mediated endocytosis at synapses:implications for neurodegenerative diseases

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Neural Regeneration Research 2018年第4期13卷 647-648页

作者： Audrey T.Medeiros Luigi Bubacco Jennifer R.Morgan The Eugene Bell Center for Regenerative biology and Tissue Engineering Marine Biological Laboratory Department of biology University of Padova

α-Synuclein causes synaptic pathologies in several neurodegenerative diseases：Parkinson’s disease（PD）is a neurodegenerative disease that impacts the lives of millions of people worldwide.A pathological hallmark of PD,as well as dementia with Lewy bodies（DLB）and several Alzheimer’s disease variants,is the appearanceof intracellular inclusions called Lewy bodies, which contain high levels of aggregated α-synuclein,

关键词： synuclein on clathrin-mediated endocytosis at synapses:implications for neurodegenerative diseases Impacts of increased

Inhibitory activity of an extract from a marine bacterium Halomonas ***07 against the red-tide microalga Gymnodinium sp.(Pyrrophyta)

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Chinese Journal of Oceanology and Limnology 2013年第6期31卷 1241-1247页

作者：刘娟李富超刘玲姜鹏刘兆普 Key laboratory of marine biology Jiangsu ProvinceNanjing Agricultural University Key laboratory of Experimental marine biology Institute of OceanologyChinese Academy of Sciences Nantong R&D Center of marine Science and Technology Institute of OceanologyChinese Academy of Sciences

In recent years,red tides occurred frequently in coastal areas *** methods based on the use of clay,copper sulfate,and bacteria have been successful in controlling red tides to some *** a new defensive agent,marine microorganisms are important sources of compounds with potent inhibitory bioactivities against red-tide microalgae,such as Gymnodinium sp.(Pyrrophyta).In this study,we isolated a marine bacterium,HSB07,from seawater collected from Hongsha Bay,Sanya,South China *** on its 16S rRNA gene sequence and biochemical characteristics,the isolated strain HSB07 was identified as a member of the genus Halomonas.A crude ethyl acetate extract of strain HSB07 showed moderate inhibition activity against Gymnodinium *** a bioactive prescreening *** extract was further separated into fractions A,B,and C by silica gel column *** B and C showed strong inhibition activities against *** is the first report of inhibitory activity of secondary metabolites of a Halomonas bacterium against a red-tide-causing microalga.

关键词： red tides marine bacteria Gymnodinium Halomonas inhibition activity

Single nucleotide polymorphisms in intron 1 and intron 2 of Larimichthys crocea growth hormone gene are correlated with growth traits

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Chinese Journal of Oceanology and Limnology 2012年第2期30卷 279-285页

作者：倪静尤锋许建和徐冬冬文爱韵吴志昊徐永立张培军 Key laboratory of Experimental marine biology Institute of OceanologyChinese Academy of Sciences Key laboratory of marine Biotechnology of Jiangsu Province Huaihai Institute of Technology Key laboratory of Mariculture and Enhancement Marine Fishery Institute of Zhejiang Province

The growth hormone gene (GH) affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms (SNPs) in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 (G A) that was negatively correlated with body height and positively correlated with standard length/body height (P 0.05). Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 (T C). The CD genotype had a significantly positive correlation with both weight and total length (P 0.01). These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection.

关键词： large yellow croaker (Larimichthys crocea) growth hormone gene (GH) PCR-single strandconformation polymorphism (PCR-SSCP) single nucleotide polymorphism (SNP) growth trait

Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5

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Acta Oceanologica Sinica 2019年第6期38卷 65-74页

作者： Yaxi Chao Shuyan Wang Suqi Wu Jiaqiang Wei Hao Chen Key laboratory of marine Bioactive Substances First Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China laboratory for marine biology and Biotechnology Pilot National Laboratory for Marine Science and Technology (Qingdao)Qingdao 266237China

Seven bacterial clones with alginate-utilizing activity were isolated from rotten kelp. By activity test, the Vibrio sp. QD-5 with the potential alginate-degrading capability was chosen to carry out the draft genome sequencing, and the result showed that the Vibrio sp. QD-5 containing an alginate lyase gene cluster. One of these genes, aly-IV, was cloned and characterized for the first time. After overexpression, Aly-IV, with a molecular mass of about 62 kDa and a theoretical isoelectric point (pI) of 5.12, was purified to a specific activity of 1 256.78 U/mg and showed highest activity at 35°C in the Tris-HCl buffer at pH of 8.9. Moreover, the enzyme activity was enhanced by the metal ions of Na+, K+ and Mg2+ under certain concentration. Aly-IV degraded favorably polyG blocks in an endo-type, yielding monomer and dimer as the main products. Due to its high substrate specificity, Aly-IV could be used as a potential tool for production of polyG oligosaccharides with low degree of polymerization (DP) and for determining the fine structure of alginate.

关键词： alginate lyase Vibrio sp. alginate poly-α-L-guluronic acid lyase oligosaccharides

Changes of melatonin and its receptors in synchronizing turbot(Scophthalmus maximus)seasonal reproduction and maturation rhythm

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Acta Oceanologica Sinica 2022年第1期41卷 84-98页

作者： Chunyan Zhao Shihong Xu Yifan Liu Chengcheng Feng Yongshuang Xiao Yanfeng Wang Qinghua Liu Jun Li School of marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Key laboratory of Experimental marine biology Centre for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Southern marine Science and Engineering Guangdong laboratory(Guangzhou) Guangzhou 511458China marine biology and Biotechnology laboratory Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

In most fish,reproduction is seasonal or periodic under the suitable *** turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause changes in the spawning *** this study,to characterize the regulation of reproductive physiology following light signals,three melatonin receptors(Mtnr)investigated in turbot were named sm Mtnr1,sm Mtnr2,and sm Mtnr1 *** expression profiles demonstrated that Mtnr m RNAs were concentrated in the brain(as detected in the hypothalamus(Hy)and mesencephalon(Me)),gonad and *** most abundant Mtnr1 and Mtnr2 m RNA expression levels were detected in the central nervous system at the beginning of the breeding season,suggesting that Mtnr1 and Mtnr2 may play vital roles in the regulation of turbot gonadal *** addition,the melatonin profiles gradually increased and reached to the highest level at the spawning stage,indicating that melatonin is a potent hormone in the regulation of fish oocyte growth and *** results of this study suggested that melatonin is the primary factor that transduces the light signal and regulates the physiological functions of turbot seasonal ***,the results of this study may establish a foundation for further research seeking to identify fish melatonin receptors involved in the gonadal development and gamete maturation.

关键词： turbot brain melatonin melatonin receptors seasonal reproductive development

Histo-blood group antigens in Crassostrea gigas and binding profiles with GⅡ.4 Norovirus

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Journal of Oceanology and Limnology 2018年第4期36卷 1383-1391页

作者： MA Liping LIU Hui SU Laij in ZHAO Feng ZHOU Deqing DUAN Delin Key laboratory of Experimental marine biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Key laboratory for Sustainable Utilization of marine Fisheries Resources Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China laboratory for marine biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

Noroviruses(NoVs) are the main cause of viral gastroenteritis outbreaks worldwide, and oysters are the most common carriers of NoV contamination and transmission. NoVs bind specifically to oyster tissues through histo-blood group antigens(HBGAs), and this facilitates virus accumulation and increases virus persistence in oysters. To investigate the interaction of HBGAs in Pacific oysters with GⅡ.4 NoV, we examined HBGAs with ELISAs and investigated binding patterns with oligosaccharide-binding assays using P particles as a model of five GⅡ.4 NoV capsids. The HBGAs in the gut and gills exhibited polymorphisms. In the gut, type A was detected(100%), whereas type Leb(91.67%) and type A(61.11%) were both observed in the gills. Moreover, we found that seasonal NoV gastroenteritis outbreaks were not significantly associated with the specific HBGAs detected in the oyster gut and gills. In the gut, we found that strain-2006 b and strain-96/96 US bound to type A and H1 but only weakly bound to type Leb; in contrast, the Camberwell and Hunter strains exhibited weak binding to types H1 and Ley, and strain-Sakai exhibited no binding to any HBGA type. In the gills, strain-96/96 US and strain-2006 b bound to type Leb but only weakly bound to type H1; strains Camberwell, Hunter, and Sakai did not bind to oyster HBGAs. Assays for oligosaccharide binding to GⅡ.4 NoV P particles showed that strain-95/96 US and strain-2006 b strongly bound to type A, B, H1, Leb, and Ley oligosaccharides, while strains Camberwell and Hunter showed weak binding ability to type H1 and Ley oligosaccharides and strain-Sakai showed weak binding ability to type Leb and Ley oligosaccharides. Our study presents new information and enhances understanding about the mechanism for NoV accumulation in oysters. Further studies of multiple NoV-tissue interactions might assist in identifying new or improved strategies for minimizing contamination, including HBGA-based attachment inhibition or depuration.

关键词： Crassostrea gigas norovirus histo-blood group antigen binding