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Journal of Oceanology and Limnology 2020年第2期38卷 571-578页

作者： JIANG Fengjuan YUE Xin ZHANG Shujing YU Jiajia WANG Rui LIU Baozhong WANG Hongxia Key laboratory of Experimental marine biology Institute of OceanologyCenter for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China laboratory for marine biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266000China University of Chinese Academy of Sciences Beijing 100049China

Gene expression variation can be considered as a phenotype,and it plays an important role in both acclimation and ***,genetic variation of gene expression received much less attention than traditional commercial traits in *** estimate the genetic variation and heritability of gene transcription in clam Meretrix petechialis,five Vibrio resistance-related genes were selected for gene expression analysis in the digestive gland,and an animal linear model was used to analyze data from quantitative real-time PCR(qRT-PCR).Among the five genes,BIRC7 showed significant additive genetic variations,the heritability of this gene of 12-month-and 15-month-old clams were 0.84±0.32 and 0.91±0.34,*** heritability of other four genes(Bax,NFIL3,Big-Def,and CTL9)expression were low-tomoderate but not significantly ***,no significant phenotypic and genetic correlations between the BIRC7 transcription trait and body size were *** study highlights that certain gene expression variation is heritable and provides a reference for indirect selection of *** with high Vibrio resistance.

关键词： Meretrix petechialis transcription trait body size heritability genetic correlation

Temperature-regulated typeⅡgrass carp reovirus establishes latent infection in Ctenopharyngodon idella brain

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Virologica Sinica 2023年第3期38卷 440-447页

作者： Rui Jiang Jie Zhang Zhiwei Liao Wentao Zhu Hang Su Yongan Zhang Jianguo Su College of Fisheries Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan430070China laboratory for marine biology and Biotechnology Pilot National Laboratory for Marine Science and TechnologyQingdao266237China

Grass carp reovirus(GCRV)causes extensive infection and death in grass carp and black carp fingerlings,with a highly seasonal *** studies suggested that GCRV can become latent after primary *** this study,we investigated type II GCRV(GCRV-II)latency in asymptomatic grass carp with GCRV infection or exposure *** found that during latent infection,GCRV-II was detectable only in the brain of grass carp,unlike the multi-tissue distribution observed in natural ***-II only caused damage to the brain during latent infection,while in natural infection,brain,heart,and eye tissues had relatively higher viral *** also discovered viral inclusion bodies in infected fish ***,GCRV-II distribution in grass carp was notably affected by ambient temperature,with the virus targeting the brain only during low temperatures and multi-tissue distribution during high *** study provides insights into the mechanisms of GCRV-II latent infection and reactivation and contributes to the prevention and control of GCRV pandemics.

关键词： Grass carp Grass carp reovirus(GCRV) Latent infection Brain

C_(17)-fengycin B,produced by deep-sea-derived Bacillus subtilis,possessing a strong antifungal activity against Fusarium solani

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Journal of Oceanology and Limnology 2021年第5期39卷 1938-1947页

作者： Weixiang LIU Chaomin SUN CAS and Shandong Province Key laboratory of Experimental marine biology Institute of OceanologyCenter for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China laboratory for marine biology and Biotechnology Pilot NationalLaboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Root rot disease caused by Fusarium solani is the most devastating disease of the tomato and legume crops in *** metabolites of Bacillus species can inhibit many fungal *** this study,the metabolites of deep-sea-derived bacterium Bacillus subtilis 2 H11 can significantly inhibit the growth of *** metabolite C_(17)-fengycin B,one of the cyclic lipopeptides,was identified by the combination of silica column chromatography,high-performance liquid chromatography(HPLC),high-energy collision induced dissociation mass spectrometry(HCD-MS)and tandem mass spectrometry(HCD-MS/MS).The results of scanning electron microscopy(SEM)and transmission electron microscopy(TEM)showed that C_(17)-fengycin B could destroy the structure of the hyphae and spores of *** antifungal activities of C_(17)-fengycin B against *** were tested at concentrations ranging from 0.05 mg/mL to 0.20 mg/*** results indicated that C_(17)-fengycin B inhibited the growth of *** with antifungal index of 89.80%at 0.20 mg/mL,and the antifungal activity of C_(17)-fengycin B was further verified by the pot *** addition,the cytotoxicity experiment showed that C_(17)-fengycin B had good biocompatibility and was a potential candidate for the development of biocontrol pesticide in the future.

关键词： Bacillus species lipopeptide fengycin antifungal pesticide

Zebrafish as a Model for Human Ciliopathies

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Journal of Genetics and Genomics 2016年第3期43卷 107-120页

作者： Zhu Song Xiaoli Zhang Shuo Jia Pamela C.Yelick Chengtian Zhao Institute of Evolution & marine Biodiversity College of Marine Life Science Ocean University of China Department of Orthodontics Division of Craniofacial and Molecular Genetics Tufts University laboratory for marine biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

Cilia, microtubule-based structures found on the surface of almost all vertebrate cells, play an array of diverse biological functions. Abnormal ciliary axonemal structure and function can result in a class of genetic disorders that are collectively termed ciliopathies. Model organisms, including Chlamydomonas reinhardtii and Caenorhabditis elegans have been widely used to study the complex genetic basis of ciliopathies. Here, we review the advantages of the zebrafish as a vertebrate model for human ciliopathies. We summarize the features of zebrafish cilia, and the major findings and contributions of the zebrafish model in recent studies of human ciliopathies. We also discuss the new genome editing approaches being efficiently used in zebrafish, and the exciting prospects of these approaches in modeling human ciliopathies.

关键词： Cilia Zebrafish Ciliopathy Disease model

The carotenoid characteristics of the important wild shrimp Trachysalambria curvirostris(Stimpson,1860) in China

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Journal of Oceanology and Limnology 2019年第2期37卷 706-712页

作者： SU Fang LIU Jianguo CAS Key laboratory of Experimental marine biology Center for Ocean Mega-Science Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China laboratory for marine biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China

Trachysalambria curvirostris is an economically important shrimp in the coastal waters of the Western Indo-Pacific. However, there is no information about its carotenoid composition and distribution. The carotenoid profiles including concentration, composition, molecular forms (free or esterified) and configuration (geometrical and optical isomers) of raw and dried T. curvirostris (Stimpson, 1860) were qualitatively and quantitatively assessed. In the raw shrimp, astaxanthin and β-carotene were the two dominant carotenoids, which contributed 75.18%–88.86% and 2.86%–16.83% of the total carotenoid content. Traces of echinenone, canthaxanthin and astacene were also detected. The carotenoid content in the waste (80.28 mg/kg dry weight) was nearly two-fold higher than that in the meat (49.69 mg/kg dry weight). Of the total astaxanthin in these shrimp, 85.18%–90.59% was esterified. All- trans astaxanthin was the predominant geometrical isomer followed by 13- cis , 9- cis , di- cis and 15- cis isomers. A mix of three stereoisomers was found in all parts and the percentages of meso (3 S , 3’ R ) and (3 S , 3’ S ) forms were significantly higher than the (3 R , 3’ R ) isomer. Compared to the carotenoid content of the raw shrimp, only 26.17 mg/kg remained in the dried shrimp. Nearly half of the carotenoids were lost during the drying process. Substantial hydrolysis and isomerization also occurred.

关键词： Trachysalambria curvirostris carotenoids astaxanthin dried shrimp

A bacterial pathogen infecting gametophytes of Saccharina japonica(Laminariales,Phaeophyceae)

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Chinese Journal of Oceanology and Limnology 2013年第2期31卷 366-373页

作者：彭艳婷李伟 College of marine Life Science Ocean University of China Key laboratory of Experimental marine biology Institute of OceanologyChinese Academy of Sciences

A newly identified bacterial disease of kelp(Saccharina japonica) gametophytes was found in clone *** is characterized by swollen gametophyte cells in the early period of infection followed by filamentous *** alginolytic marine bacterium referred to as A-1 was isolated from the diseased *** the basis of 16S rDNA sequencing and morphological,physiological and biochemical characteristics,the bacterium was identified as a strain of the genus *** testing Koch's postulates,Alteromonas sp.A-1 was further confirmed as the *** infection process was also investigated using both scanning electron and light microscopy.

关键词： Saccharinajaponica gametophyte pathogen bacteria Alteromonas

Reproductive toxicity assessment of benzo[a]pyrene in the marine polychaete Perinereis nuntia

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Chinese Journal of Oceanology and Limnology 2017年第4期35卷 867-873页

作者：吴清洋王树启陈晓鹏李平 Guangdong Provincial Key laboratory of marine biology Shantou University Administration of Ocean and Fisheries of Shantou

Benzo[a]pyrene （B[a]P） is an increasingly present marine environmental pollutant, yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited. To test the reproductive toxicity of B[a]P on polychaetes, Perinereis nuntia was exposed to B[a]P-contaminated artificial seawater and sexual maturation, the sex ratio, number of eggs spawned, fertilization and hatching rated, as well as vitellogenin （VTG） mRNA expression levels were analyzed. A low concentration of B[a]P （2.5 gg/L） had no effects on the rate of sexual maturation, spawning, or fertilization but significantly increased the sex ratio （female： male） from 1.6±0.15：1 to 2.3±0.18：1, inhibited hatching rate by 27%, and significantly increased VTG mRNA expression level by 3.7-fold following a 60-day exposure, compared with those in the solvent controls. A higher concentration of B[a]P （25 μg/L） caused more serious effects; sexual maturation, fertilization success, and hatching decreased by 31%, 17% and 46%, respectively, and the sex ratio （female： male） and VTG mRNA gene expression level increased by 54% and 7.1-fold, respectively. These results demonstrate that sublethal concentrations of B[a]P negatively affect reproductive performance of the sandworm P. nuntia.

关键词： Perinereis nuntia benzo[a]pyrene reproductive biology vitellogenin

Characterization of the squalene-rich Botryococcus braunii Abt02 strain

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Journal of Oceanology and Limnology 2019年第2期37卷 675-684页

作者： CAO Min ZHANG Fangfang MAO Yunxiang KONG Fanna WANG Dongmei College of marine Life Sciences Ocean University of China Qingdao 266003 China Key laboratory of marine Genetics and Breeding (OUC) Ministry of Education Qingdao 266003 China laboratory for marine biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266000 China

Botryococcus braunii is widely studied due to its high hydrocarbon content. In this study, B . braunii Abt02 was subjected to several analyses, including cytological observation, hydrocarbon composition analysis by gas chromatography mass spectrometry (GC-MS), phylogenetic identification using known races (A, B and L) of B . braunii strains based on their 18S rDNA sequences, and qPCR-based investigation of transcript accumulation levels of hydrocarbon biosynthesis-related enzymes (DXS, MCS, DLS, SQS) during diff erent growth phases (lag phase, log phase, early stationary growth phase, late stationary growth phase) under nitrogen-replete and nitrogen-depleted growth conditions, respectively. Based on cytological observation and on the 18S rDNA phylogenetic analysis, strain Abt02 was assigned to race B. Analysis of the strain’s chemical composition showed that the B . braunii Abt02 contained high levels of hydrocarbons, which accounted for 43.75% of the cell’s dry weight. Of these hydrocarbons, squalene and its derivatives accounted for up to 87.54%. In addition, all four enzymes investigated were expressed at higher levels during the log growth phase under nitrogen depleted conditions than under nitrogen replete conditions.

关键词： Botryococcus braunii cytological observation phylogenetic analysis hydrocarbon components qPCR

Antioxidative and antimicrobial activities of intertidal seaweeds and possible effects of abiotic factors on these bioactivities

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Journal of Oceanology and Limnology 2018年第6期36卷 2243-2256页

作者： XU Peihang TAN Huaqiang JIN Weiguang LI Yanfei LI Ping LIU Wenhua marine biology Institute Shantou University Guangdong Provincial Key laboratory of marine biology Shantou University STU-UNIVPM Joint Algal Research Center College of SciencesShantou University

To overcome acute physiological stress from frequent exposure to high irradiance levels and emersion stress, seaweeds colonizing intertidal zones generate valuable secondary metabolites. Their chemical composition is influenced by spatial changes in environmental parameters, and bioactivities closely linked to specific compounds change accordingly. We measured antioxidative and antimicrobial activities of 26 species in ten intertidal zones of eastern Guangdong, China, and examined the possible effects of abiotic factors. Most brown algae exhibited higher antioxidative activity and total phenol content than red algae and green algae, while most brown algae and green algae revealed more efficient antimicrobial activity than red algae. Their activities were also af fected by the environment of their intertidal habitats. Similar antioxidative ability or total phenol content were found in seaweeds settled in high-, middle-and low-tide zones, while more positively antimicrobial ability was discovered in seaweeds colonizing the low-tide zone than in those in middle-and high-tide zones. These differences were mainly caused by the different stress levels of sun exposure, nutrition and desiccation, as well as temperature and salinity fluctuations in various tidal regions. Seaweeds colonizing coastal waters and experiencing stresses such as low salinity, limited dissolved oxygen(DO) or rich nutrition exhibited superior antioxidative ability and total phenol content. This result was further strengthened by the finding that the antioxidative ability and total phenol content of Ulva fasciata were positively af fected by nutrition and negatively influenced by DO or salinity. The antioxidative activity and total phenol content of S argassum vachellianum were also positively af fected by ammonium-nitrogen. No similar trend was found in the antimicrobial activity of seaweeds. Our results suggested an effect of abiotic factors on the antioxidative and antimicrobial activities of inter

关键词： abiotic stress antioxidant activity antibacterial activity phenol content intertidal zone macroalgae

Photosynthesis and growth responses of pea Pisum sativum L. under heavy metals stress

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Journal of Environmental Sciences 2009年第11期21卷 1552-1556页

作者： Sabrine Hattab Boutheina Dridi Lassad Chouba Mohamed Ben Kheder Hamadi Bousetta laboratory of Biochemistry and Environmental Toxicology laboratory of marine biology National Institute of Sea Sciences and Technologies Lagoulette Technical Centre of Organic Agriculture

The present work aimed to study the physiological effects of cadmium （Cd） and copper （Cu） in pea （Pisum sativum）. Pea plants were exposed to increasing doses of cadmium chloride （CdCl2） and copper chloride （CuCl2） for 20 d. The examined parameters, namely root and shoot lengths, the concentration of photosynthetic pigments and the rate of photosynthesis were affected by the treatments especially with high metals concentrations. The analysis of heavy metals accumulation shows that leaves significantly accumulate cadmium for all the tested concentrations. However, copper was significantly accumulated only with the highest tested dose. This may explain the higher inhibitory effects of cadmium on photosynthesis and growth in pea plants. These results are valuable for understanding the biological consequences of heavy metals contamination particularly in soils devoted to organic agriculture.

关键词： Pisum sativum heavy metals photosynthesis growth