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Structure-function Aspects of Extracellular Leucine-rich Repeat-containing Cell Surface Receptors in Plants

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Journal of Integrative Plant Biology 2013年第12期55卷 1212-1223页

作者： Zhao Zhang Bart P.H.J.Thomma laboratory of Phytopathology Wageningen University Centre for BioSystems genomics

Plants exploit several types of cell surface receptors for perception of extracellular signals, of which the extracellular leucine-rich repeat （eLRR）-containing receptors form the major class. Although the function of most plant eLRR receptors remains unclear, an increasing number of these receptors are shown to play roles in innate immunity and a wide variety of developmental processes. Recent efforts using domain swaps, gene shuffling analyses, site-directed mutagenesis, interaction studies, and crystallographic analyses resulted in the current knowledge on ligand binding and the mechanism of activation of plant eLRR receptors. This review provides an overview of eLRR receptor research, specificallysummarizing the recent understanding of interactions among plant eLRR receptors, their co-receptors and corresponding ligands. The functions of distinct eLRR receptor domains, and their role in structure, ligand perception and multimeric complex formation are discussed.

关键词： eLRR receptor-like kinase (RLK) receptor-like protein (RLP) pathogen-associated molecular pattern (PAMP) effector peptide ligand brassinosteroid CLE peptide.

Host-induced gene silencing of multiple pathogenic factors of Sclerotinia sclerotiorum confers resistance to Sclerotinia rot in Brassica napus

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The Crop Journal 2022年第3期10卷 661-671页

作者： Jian Wu Shengliang Yin Li Lin Dongxiao Liu Sichao Ren Wenjing Zhang Wencheng Meng Peipei Chen Qinfu Sun Yujie Fang Cunxu Wei Youping Wang Key laboratory of Plant Functional genomics of the Ministry of Education Yangzhou UniversityYangzhouChina Jiangsu Key laboratory of Crop genomics and Molecular Breeding Yangzhou UniversityYangzhouChina

Sclerotinia sclerotiorum is generally considered one of the most economically damaging pathogens in oilseed rape(Brassica napus).Breeding for Sclerotinia resistance is challenging,as no immune germplasm available in *** is desirable to develop new breeding *** the present study,hostinduced gene silencing(HIGS),developed based on RNA interference(RNAi),was applied to protect *** from *** *** pathogenicity genes,the endo-polygalacturonase gene(Ss PG1),cellobiohydrolase gene(Ss CBH),and oxaloacetate acetylhydrolase gene(Ss OAH1),were chosen as HIGS ***-incubation of synthesized double-stranded RNAs(ds RNAs)with *** in liquid medium significantly reduced the transcript levels of the target *** to plant surfaces of ds RNA targeting the three genes conferred effective protection against *** transgenic *** plants expressing small interfering RNAs with sequence identity to Ss PG1,Ss CBH,and Ss OAH1 were *** transgenic *** prevented the expression of *** target genes,slowed pathogenicity-factor accumulation,impeded fungal growth,and suppressed appressorium formation,thereby conferring resistance to *** silencing of Ss PG1,Ss CBH,and Ss OAH1 by stable expression of a chimeric hairpin RNAi construct in *** led to enhanced protection phenotypes(with disease lesion size reduced by 36.8%–43.7%).We conclude that HIGS of pathogenic-factor genes of *** is a promising strategy for controlling Sclerotinia rot in oilseed rape.

关键词： Sclerotinia sclerotiorum Brassica napus SIGS HIGS RNAi

OsSCE1 Encoding SUMO E2-Conjugating Enzyme Involves in Drought Stress Response of Oryza sativa

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Rice science 2018年第2期25卷 73-81页

作者： Dini NURDIANI Dwi WIDYAJAYANTIE Satya NUGROHO laboratory of genomics and Crop Improvement Research Center for Biotechnology Indonesian Institute of Sciences

Small ubiquitin-like modifier(SUMO)-conjugating enzymes are involved in post-translational regulatory processes in eukaryotes, including the conjugation of SUMO peptides to protein substrate(SUMOylation). SUMOylation plays an important role in improving plant tolerance to abiotic stress such as salt, drought, heat and cold. Herein, we reported the isolation of OsSCE1(LOC_Os10 g39120) gene encoding a SUMO-conjugating enzyme from rice(Oryza sativa cv. Nipponbare) and its functional validation in response to drought stress. The E2 enzyme, Os SCE1, is one of three key enzymes involved in the conjugation of SUMO to its target proteins. Activated SUMO is transferred to the cysteine of an E2 enzyme and then to the target lysine residue of the substrate, with or without the help of an E3 SUMO ligase. Expression of OsSCE1 was strongly induced by polyethylene glycol 6000(PEG6000) treatment, which suggested OsSCE1 may be involved in the drought stress response. Overexpression of OsSCE1(OsSCE1-OX) in Nipponbare reduced the tolerance to drought stress. Conversely, the drought tolerance was slightly improved by the knockdown of OsSCE1(OsSCE1-KD). These results were further supported by measurement of proline content in OsSCE1-OX and OsSCE1-KD transgenic lines under induced drought stress, which showed OsSCE1-KD transgenic lines accumulated higher proline content than the wild type, whereas OsSCE1-OX line had lower proline content than the wild type. These findings suggested OsSCE1 may play a role as a negative regulator in response to drought stress in rice.

关键词： Oryza sativa drought stress small ubiquitin-like modifier SUMO-conjugating enzyme proline content gene expression

Phylogeny of genera Laminaria and Saccharina(Laminariales, Phaeophyceae) based on three molecular markers

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Acta Oceanologica Sinica 2014年第9期33卷 139-151页

作者： CHI Shan QIAN Hao LI Tianyong WANG Xumin LIU Cui REN Lei TANG Xuexi LIU Tao Ocean University of China Beijing Key laboratory of Functional genomics for Dao-di Herbs Beijing Institute of Genomics Chinese Academy of Sciences Chinese Academy of Sciences Key laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine Technologies Beijing Institute of Genomics Chinese Academy of Sciences

In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera： Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Lami- nariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae-Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxi- dase I （COI） sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer （ITS） and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina.

关键词： Saccharina Laminaria phylogeny COI rbcL ITS

Development of a functional cell-based HTS assay for identification of NKCC1-negative modulators

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Chinese Science Bulletin 2014年第7期59卷 630-638页

作者： Yanqiu Bai Lianghui Ma Shiyou Li Beijing Institute of genomics Chinese Academy of Sciences University of Chinese Academy of Sciences laboratory of Disease genomics and Individualized Medicine Beijing Institute of Genomics Chinese Academy of Sciences

Na–K–Cl cotransporter 1(NKCC1) cotransports Na+, K+, and Cl-ions across the plasma membrane into cells. Accumulation of Cl-ions in dorsal root ganglion neurons induces depolarizing GABAA receptors, which mediate presynaptic inhibition and filtration of sensory noise. The activity of the Na–K–Cl cotransporter is modulated by high-dose loop diuretics, such as furosemide and bumetanide. To identify NKCC1 modulators, we developed a functional cell-based assay feasible for highthroughput screening(HTS), in which the activity of NKCC1 was detected by a BTC-AM dye-based thallium transportation assay. We demonstrated that the influx of Tl?was mediated by NKCC1, which required the existence of Cl-ions and could be inhibited by bumetanide and furosemide. Our results demonstrated that the assay was stable, reproducible, and suitable for HTS of negative modulators for NKCC1.

关键词：细胞膜测定法 HTS 调节剂识别背根神经节神经元 GABAA受体转运蛋白

Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si Wang Weiqi Zhang Jing Qu Guang-Hui Liu State Key laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

Latest notable achievements in genomics

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Science China(Life Sciences) 2012年第7期55卷 645-648页

作者： WU JiaYan XIAO JingFa YU Jun Key laboratory of genomics Science and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100029 China

Within the past 10 years, the progress of genomics development in China exhibited a frogleap in terms of scale, quality, organization, and international collaboration. Human genome study has entered a new phase of interdisciplinarity in the history of life science in China. The rise of genomics relied on the development of technology and its integration with other academic disciplines, particularly in the following three areas. First, DNA sequencing technology has been improving its productivity and efficiency at an exponential growth rate. Second, bioinformatics supports the system of data collection, administration, annotation,

关键词：基因组学 DNA测序技术成绩人类基因组生物信息学国际合作生命科学数据采集

Maize gets an iron boost:Biofortification breakthrough holds promise to combat iron deficiency

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Journal of Integrative Plant Biology 2024年第4期66卷 635-637页

作者： Sunil Kumar Sahu State Key laboratory of Agricultural genomics Key Laboratory of GenomicsMinistry of AgricultureBGI ResearchShenzhen 518083China BGI Research Wuhan 430074China

Micronutrient deficiencies affect more than two billion people worldwide,resulting in poor health,developmental issues,and even *** deficiency is one of the most prevalent micronutrient deficiencies worldwide and impa... 详细信息

关键词： population breakthrough holds

Inevitability or contingency: how many chromosomes do we really need?

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Science China(Life Sciences) 2019年第1期62卷 140-143页

作者： Shuangying Jiang Junbiao Dai Shenzhen Key laboratory of Synthetic genomics and Center for Synthetic genomics Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences

In an essay written by the evolutionary biologist Theodosius Dobzhansky in 1973,he pointed out that "Nothing in Biology Makes Sense Except in the Light of Evolution."An interesting phenomenon in biology is the presence of variable numbers of chromosomes in different *** several species,which possess multiple circular chromosomes or simply linear chromosomes (Baril et al. 1989;Suwanto and Kaplan,1989;Jumas-Bilak et al.,1998), most prokaryotes only possess one circular *** contrast,the genomes of eukaryotic species are usually packaged into linear chromosomes with numbers varying from one to hundreds (Crosland and Crozier,1986;Lukhtanov,2015).

关键词： Inevitability contingency evolutionary biologist