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中国植物病理学会2019年学术年会

作者： Wang Xue Yin Qiaoxiu Li Dongxue Ren Yafeng Wu Xian Jiang Shilong Song Baoan Chen Zhuo State Key laboratory breeding base of green pesticide and agricultural bioengineering Guizhou University College of Agriculture Guizhou University

Tea plant(Camellia sinensis) is a major crop in Guizhou province,*** the end of 2017,the cultivated area of tea plant covered 350 000 *** November 2018,tea leaf spot was caused a huge loss of the production of tea in Yuqing county in Guizhou *** symptoms of the infected tea leaves were brown,irregular and the center of lesion appeared to be *** some tea gardens,disease incidence of leaves was 46% to 52%,and decreased seriously the production of tea *** this study,some fungi from tea disease samples were identified,such as Phomopsis foeniculi,Diaporthe ganias,et ***,some isolates were obtained to our *** colony of GZYQ-03-02 on PDA initially was white,and then the colony was gradually changed to black after 14 days *** genes of DNA-dependent RNA polymerase II second largest subunit(RPB2),beta-tubulin 2(β-TUB2) and actin(ACT) were amplified and sequenced from the isolates,and then deposited in Genbank(Accession ***852276,MK852273 and MK852274 for the isolate GZYQ-03-02).Maximum parsimony phylogenetic analysis based on concatenated sequences of combined ACT(1-312),β-TUB2(313-506),RPB2(507-847) indicated that the isolate GZYQ-03-02 was identical to reference strains Nemania bipapillata 90080610,and the clade was supported by 99% bootstrap *** fulfill Koch’s postulates,unwounded and wounded tea leaves were inoculated with mycelial plugs on PDA,and non-symptoms were observed after 7 days of *** was known as synonym of Nemania *** caused tea stem wood decay in Sri ***,*** was also known as endophyte,which was isolated from plant hosts of Lycium chinense,Cephalotaxus,Vanilla planifolia,et *** our knowledge,we firstly isolated the fungus from tea *** mechanism of the fungus in the tea leaf spot is worth studying in the future.

关键词： Nemania bipapillata identification pathogenicity test leaf spot pathogenicity mechanism

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Pithomyces chartarum was firstly identified from tea leaf spot in Yuqing,Guizhou Province

Pithomyces chartarum was firstly identified from tea leaf sp...

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中国植物病理学会2019年学术年会

作者： Yin Qiaoxiu Wang Xue Ren Yafeng Li Dongxue Jiang Shilong Wu Xian Song Baoan Chen Zhuo State Key laboratory breeding base of green pesticide and agricultural bioengineering Guizhou University College of Agriculture Guizhou University

Leaf spot disease is an important disease of tea(Camellia sinensis) in Yuqing county,Guizhou *** symptoms were small,round or irregular *** lesion gradually enlarged to display the darker lesion margin with a bright yellowish-black *** center of lesion appeared to be *** adjacent lesions have been merged to the larger lesion and covered the margin of the *** disease takes place in tea garden during autumn on altitude from one thousand to one thousand three hundred *** incidence of leaves was estimated at 74% to 82%,depending on the *** severity on a plant basis was estimated to be 68% to 76%.In order to identify the pathogens of this disease,we isolated and identified some fungi from tea samples with the symptoms of leaf spot,and conducted the pathogenicity *** found Phoma segeticola,Didymella bellidis and Pestalotiopsis trachicarpicola cause the lesions of tea leaves by Koch’s *** addition,we also isolated and identifed the isolate of GZYQ4-1-d from the samples of tea *** colony of GZYQ4-1-d on PDA was regular and round,flat growing,sometimes floccose,*** time,the colony became grey to dark grey because the pigment of mycelium *** are typically pigmented,muriform,sometimes echinulate,usually with 3 transverse septa and with 0-2 longitudinal septa,measured to(17.16-28.38) μm×(12.57-18.04) μ*** internal transcribed spacer(ITS) regions 1 and 2 was used to amplify and sequenced,and the sequence revealed 100% identity with *** by Blastn *** pathogenicity test was conducted result indicated that the strains can induce leaf spot on leaves of *** leaves of the tea plant varieties of Moss tea and Fuding Dabaicha were inoculated with mycelial plugs on PDA or the spore suspension(1×10 spores per mL) using the methods of unwound,puncture or *** symptom has not been observed on inoculated leaves for three ***,some literatures reported t

关键词： Pithomyces chartarum identification pathogenicity test leaf spot pathogenicity mechanism

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Cladosporium cladosporioides was firstly identified from tea leaf spot disease in Yuqing,Guizhou Province,China

Cladosporium cladosporioides was firstly identified from tea...

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中国植物病理学会2019年学术年会

作者： Wang Xue Yin Qiaoxiu Li Dongxue Ren Yafeng Wu Xian Jiang Shilong Wang Yong Song Baoan Chen Zhuo State Key laboratory breeding base of green pesticide and agricultural bioengineering Guizhou University College of Agriculture Guizhou University

Tea leaf spot disease was an important disease in Yuqing county,Guizhou *** symptoms were small grey dark elliptical *** lesion was gradually enlarged with irregular shape and the center of lesion appeared to be *** incidence of leaves was estimated at 46% to 52%,depending on the *** disease can seriously decrease the production and quality of tea *** this study,we isolated and identified some fungi from tea samples with the symptoms of leaf spot,and then conducted the pathogenicity *** example,Nigrospora oryzae,Nigrospora sphaerica,et al were identified from these leaves *** addition,we further identified some isolates from the samples of tea leaves,with the similar morphological *** colony of GZYQ-08-01 on PDA initially was grey olivaceous,and then changed to be grey or olivaceous black,which showed a velvety *** conidia were numerous,elliptical,limoniform,branched chains,aseptate,and measured 5.66±3.16(3.44 to 26.5) μm×3.21±0.61(2.34 to 5.02) μm(n=50).The internal transcribed spacer(ITS) regions,the translation elongation factor(TEF-1α) and actin(ACT) sequences were amplified and sequenced from the isolates,and then deposited in Genbank(Accession ***852271,MK852273,MK852272,and for GZYQ-08-01).The phylogenetic tree was constructed by PAUP v 4.0 b10 software coupled with the Maximum Parsimony *** isolate was identical to reference strains Cladosporium cladosporioides CBS15038,and the clade was supported by 100%bootstrap *** mycelial plugs on PDA or the spore suspension(1×10 spores per mL) were inoculated to the tea leaves using the methods of unwound,puncture or *** lesions were not observed on tea leaves after 7 days *** literatures reported that the fungus caused blossom blight of strawberry,leaf spot of Alstroemeria aurea,raceme blight of macadamia *** *** was involved with the formation of leaf spot in Yuqing county,Gui

关键词： Cladosporium cladosporioides identification pathogenicity test leaf spot pathogenicity mechanism

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Absolute configuration of asymmetric α-aminophosphonates obtained through organocatalytic hydrophosphonylation of in situ generated and pregenerated Imines

Absolute configuration of asymmetric α-aminophosphonates ob...

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中国化学会第三届全国分子手性学术研讨会

作者： Pinaki S.Bhadury State Key laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agriculture BioengineeringMinistry of EducationCenter for Research and Development of Fine ChemicalsGuizhou University

＜正＞The asymmetric organocatalytic hydrophosphonylation of an imine with a phosphite is a powerful synthetic tool for the construction of carbon-phosphorus bonds in organic chemistry,providing efficient access to chiral a-aminophosphonates,which show significant biological activity and have applications in the pharmaceutical *** the bioactivity ofα-aminophosphonates is greatly influenced by the absolute configuration of the chiral carbon center,a series of these compounds were synthesized and evaluated in our laboratory using chiral Bronsted acid organocatalyst derived from（R）-or（S）-BINOL in a typical hydrophosphonylation reaction of different pregenerated imines with dialkyl *** order to ascertain the absolute configurations of the a-aminophosphonates,the sign of optical rotation of these products was compared with those reported by earlier authors who assigned a positive value of optical rotation to the（R） enantiomer and a negative value to the（S） enantiomer *** the basis of the observed optical rotation in our experiment,the absolute configurations of a-aminophosphonates derived from（R）-and（S）-BINOL were assigned as（R） and（S） respectively by comparison with the literature ***,an entirely reverse trend in enantioselectivity has been noticed for similar reaction using a three component（in situ generated imine） Kabachink-Fields reaction in presence of similar Bronsted acid chiral ***α-aminophosphonates from（R） catalyst revealed an（S） configuration whereas those derived from（S） catalyst revealed an（R） *** contrasting behavior may be attributed to different mechanistic pathway through which asymmetric induction is induced by bifunctional organocatalyst in typical three component one pot reaction vis a vis hydrophosphonylation of pregenerated *** propose to take up the study of unequivocal assignment of absolute configurations of title compounds in these reactions by generating suitable crystals o

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Biological characteristics of the pathogen Phoma segeticola *** causing a new tea foliage disease

Biological characteristics of the pathogen Phoma segeticola ...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Wei Wei Ren Yafeng Li Dongxue Zhao Xiaozhen Song Baoan Chen Zhuo College of Agriculture Guizhou University State Key laboratory breeding base of green pesticide and agricultural bioengineering Guizhou University Guizhou Institute of Pomology

Tea leaf spot disease caused by Phoma segeticola *** always take place in the tea region with higher altitude of Guizhou Province during the cold *** it is lack of an effective and safe control measure,it led to a very huge loss of tea production and serious decrease in tea *** is a very significance to establish the targeting mode,screen the fungicides with higher activity,and study its action *** this study,we studied the biological characteristics of the representative strain GZSQ-4 of *** *** in *** results indicated that the strain GZSQ-4 can grow on the medium of PDA,OA and MEA,without a significant difference in the growth *** strain GZSQ-4 can produce an amount of conidia on *** was suitable for growth in 25℃ in vitro,and it had a quicker growth rate in neural-than weak acid-or mild *** strain can also grow in PDB medium with the logarithmic phase being from 36 to 120 *** nutrition can influence the growth of the strain and the formation of hyphal *** results would lay a way for the screening the fungicides with a higher activity.

关键词： Camellia sinensis Foliage disease P.segeticola var.camelliae Biological characteristics Screening fungicides

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Recent advances in formaldehyde-responsive fluorescent probes

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Chinese Chemical Letters 2017年第10期28卷 1935-1942页

作者： Zhiqiang Xu Jianhua Chen Lin-Li Hu Ying Tan Sheng-Hua Liu Jun Yin College of Chemistry Environmental Engineering Yangtze University fingzhou 434023 China The State Key laboratory breeding base-Shenzhen Key laboratory of Chemical Biology Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China Key laboratory of pesticide and Chemical Biology Ministry of Education College of Chemistry Central China Normal University Wuhan 430079 China

Formaldehyde, as one of the simplest reactive carbonyl species（RCS）, is regarded as a potential carcinogen and a sick house syndrome gas. Recent studies have shown that abnormally high levels of formaldehyde may result in cognitive decline and spatial memory deficits, asthmatic symptoms,Alzheimer＇s disease, and cancer. Due to the harmfulness of high levels of formaldehyde in nature and humans, it is of great significance to further elucidate the roles and functions of formaldehyde by a noninvasive detection approach. Fluorescence imaging has become a powerful and popular tool in monitoring bio-species owing to their high sensitivity and selectivity, excellent spatiotemporal resolution and non-invasion nature. Therefore, fluorescent probes are widely applied to track and detect formaldehyde in vitro and in vivo which have attracted more and more interest recently. This review focuses on various strategies to design the fluorescent probes for detecting formaldehyde based on different recognition groups.

关键词： Formaldehyde Fluorescent probes Bioimaging application Sensing mechanism

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A pathogenic fungus Pseudopestalotiopsis theae was firstly identified from tea leaf spot disease in Kaiyang,Guizhou Province,China

A pathogenic fungus Pseudopestalotiopsis theae was firstly i...

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中国植物病理学会2019年学术年会

作者： Ren Yafeng An Xiaoli Li Dongxue Bao Xingtao Jiang Shilong Wang Delu Song Baoan Chen Zhuo State Key laboratory breeding base of green pesticide and agricultural bioengineering Guizhou University College of Forestry Guizhou University College of Agriculture Guizhou University

Tea leaf spot disease mainly damaged young leaves and shoot of tea（Camellia sinensis）and led to a huge loss of the production of tea in Kaiyang county,Guizhou *** order to identify the causal agent of this disease,we isolated and identified the *** morphologies of the colony on PDA were undulating,with regular margin and the aerial mycelia represented woolly-cottony and pure *** conidiomata containing black conidial masses were formed on the aerial *** cells were in clusters,simple filiform,hyaline and *** represented fusiform,4-septate,measured 24.99±2.10 μm（20.25-28.52 μm） x 6.00±0.70 μm（4.83-8.56 μm）,with 3-4 apical appendages,and the three median cells were brown,whereas basal cells were *** genes of translation elongation factor 1-alpha（EFl）,the internal transcribed spacer（ITS）,28 S nrDNA（LSU） and beta-tubulin（TUB） were amplified and *** parsimony phylogenetic analysis based on concatenated sequences of combined EF1（1-356）,ITS（357-969）,LSU（970-1895） and TUB（1896-2426） indicated that the strain GZKY2018 AXL4 D was identical to reference strain Pesudopestalotiopsis theae MFLUCC12-0055,and the clade was supported by 97.3% bootstrap *** fulfill the Koch’s postulate,wounded tea leaves were inoculated with actively growing mycelia plugs on *** light brown spots were formed on inoculation site after 1-2 d of *** our knowledge,this is the first report of *** causing tea leaf spot in this region,and this work will help the management of disease in the field.

关键词： Pseudopestalotiopsis theae identification pathogenicity test

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green Plant Protection Innovation: Challenges and Perspectives

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Engineering 2020年第5期6卷 483-484页

作者： Baoan Song James NSeiber Stephen ODuke Qing XLi State Key laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China Department of Environmental Toxicology University of California at DavisDavisCA 95616USA National Center for Natural Products Research Thad Cochran Research CenterSchool of PharmacyUniversity of MississippiOxfordMS 38655USA Department of Molecular Biosciences and bioengineering University of Hawaii at ManoaHonoluluHI 96822USA

This issue of Engineering was compiled from papers presented at the First International Conference on green Plant Protection Innovation(1st ICGPPI),held in Haikou in May 2018,and the Second International Conference on green Plant Protection Innovation(2nd ICGPPI),held in Guiyang in October *** conferences were sponsored by the Chinese Academy of Engineering,and the Academic Committee of the Forum of the ICGPPI,chaired by Professor Baoan Song,organized the *** 1st ICGPPI was attended by more than 800 people from seven countries,while the 2nd ICGPPI had more than 200 attendees from nine *** wide range of topics related to more environmentally sound crop protection was of great interest to all,and the meetings provided provided an opportunity for scientists from many disciplines of crop protection to exchange and share ideas and advances.

关键词： Plant green PPI

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Polycephalomycetaceae,a new family of clavicipitoid fungi segregates from Ophiocordycipitaceae

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Fungal Diversity 2023年第3期120卷 1-76页

作者： Yuan‑Pin Xiao Yuan Bing Wang Kevin D.Hyde Gentekaki Eleni Jing‑zu Sun Yu Yang Juan Meng Hong Yu Ting‑Chi Wen State Key laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China The Engineering Research Center of Southwest Bio‑Pharmaceutical Resources Ministry of EducationGuizhou UniversityGuiyang 550025GuizhouChina Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand CAS Key laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Yunnan Key laboratory for Fungal Diversity and green Development Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Yunnan Herbal laboratory School of Ecology and Environmental ScienceYunnan UniversityKunming 650091China Innovative Institute of Plant Health Zhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225China State Key laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China The Mushroom Research Centre Guizhou UniversityGuiyang 550025China School of Pharmaceutical Engineering Guizhou Institute of TechnologyGuiyang 550003GuizhouChina

Clavicipitoid fungi comprise three families,namely Clavicipitaceae,Cordycipitaceae,and *** are found worldwide and are specialized pathogens of invertebrate,plant and fungal *** the last decade,morphology-and phylogeny-based studies on clavicipitoid fungi have *** latter have revealed that Polycephalomyces,Perennicordyceps and Pleurocordyceps consistently cluster *** genera are currently considered as members of ***,information with regard to their diversity and ecology remains *** fill this gap,we collected 29 fresh specimens from insect and fungal substrates from tropical and subtropical evergreen forests in Thailand and southwestern *** performed detailed morphological analyses and constructed photoplates for all isolated *** used extensive taxon sampling and a dataset comprising internal transcribed spacer gene region(ITS),small subunit ribosomal RNA gene region(SSU),large subunit rRNA gene region(LSU),translation elongation factor 1-alpha gene region(TEF-1α),RNA polymerase II largest subunit gene region(RPB1)and RNA polymerase II second largest subunit(RPB2)to infer order-,family and genus-level phylogenetic *** on these biphasic analyses,we segregate Polycephalomyces,Perennicordyceps,and Pleurocordyceps from Ophiocordycipitaceae and introduce the new family Polycephalomycetaceae to accomodate these three *** majority of species in this family have a vast range of insect and fungal *** sexual morph of Polycephalomycetaceae has stromatic ascomata,long stipes,thick peridium,and cylindrical secondary *** asexual morph is characterized by colonies on the host surface or synnemata with stipes on the host,one or two types of phialides,and cylindrical to fusiform *** expand the number of taxa in the new family by introducing seven new species(Polycephalomyces albiramus,Perennicordyceps lutea,Pleurocordyceps parvicapitata,Pleurocordyceps lan

关键词： 8 new taxa Ophiocordyceps Polycephalomyces Hypocreales Clavicipitoid fungi Taxonomy New family Morphology Phylogeny

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Taxonomic studies of some often over-looked Diaporthomycetidae and Sordariomycetidae

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Fungal Diversity 2021年第6期111卷 443-572页

作者： Shi-Ke Huang Kevin D.Hyde Ausana Mapook Sajeewa S.N.Maharachchikumbura Jayarama D.Bhat Eric H.C.McKenzie Rajesh Jeewon Ting-Chi Wen State Key laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China The Engineering Research Center of Southwest Bio-Pharmaceutical Resources Ministry of EducationGuizhou UniversityGuiyang 550025Guizhou ProvinceChina Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand The Mushroom Research Centre Guizhou UniversityGuiyang 550025China Institute of Plant Health Zhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225China School of Life Science and Technology University of Electronic Science and Technology of ChinaChengdu 611731China Department of Botany Goa UniversityGoaIndia Present Address:Goa Velha India Manaaki Whenua-Landcare Research Private Mail Bag 92170AucklandNew Zealand Department of Health Sciences Faculty of Medicine and Health SciencesUniversity of MauritiusReduitMauritius

Sordariomycetes is an earlier-introduced and one of the widely distributed class of *** class was initially clas-sified based on morphology in having inoperculate and unitunicate *** the development of DNA based phylogenetic analysis,several undetermined or polyphyletic members of Sordariomycetes were ***,not all species belonging to this class have been sequenced and *** are a number of species,especially those old and poorly studied ones which have never been sequenced before and not even recollected again for further taxonomic *** of the main objective in this study is to revise and update the taxonomy of several well-known early and poorly studied spe-cies whose classification are still ***,we re-examined the type materials and/or authentic specimens together to explore 74 relatively poorly-studied genera,which mainly belong to Boliniales,Calosphaeriales,Chaetosphaeriales,Jobellisiales,and Sordariales classified under Diaporthomycetidae and *** provide descriptions,notes,figures and/or drawings and discussed their phylogenetic *** a result,the monotypic Jobellisiales is transferred from Hypocreomycetidae to *** on phylogenetic analysis,the polyphyletic Lasiosphaeriaceae is divided into five families,Bombardiaceae(Apodospora,Bombardia,Bombardioidea,Fimetariella and Ramophialophora),Lasiosphaeriaceae(Anopodium,Bellojisia,Corylomyces,Lasiosphaeria,Mammaria and Zopfiella),Lasiosphaeridaceae(Lasiosphaeris),Strattoniaceae(Strattonia)and Zygospermellaceae(Episternus and Zygospermella).In addition,a new family Neoschizotheciaceae is established based on *** of the type species of Boothiella,Stel-latospora,Sulcatistroma and Tengiomyces placed them in Sordariaceae,Chaetomiaceae,Hypocreales and Coronophorales,*** classify the genera lacking molecular data based on their morphology and expect them to be recollected;that is

关键词： 43 new taxa Boliniales Calosphaeriales Helminthosphaeriaceae Jobellisiales Sordariales Sordariomycetes

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