检索结果-南通市图书馆

Identification of a new isoform of the murine Sh2d1a gene and its functional implications

Science China(Life Sciences) 2014年第1期57卷 81-87页

作者： WU LongYan LU PeiWen MA WeiWei CHU CoCo XU HePing QI Hai Tsinghua-Peking Center for Life Sciences Laboratory of Dynamic Immunobiology School of Medicine School of Life SciencesTsinghua University

Signaling lymphocytic activation molecule （SLAM）-associated protein （SAP） is a Src homology （SH） domain 2-containing in- tracellular adaptor protein that is predominantly expressed in the hematopoietic system by T lymphocytes and NK cells. SAP protein is encoded by the SH2DIA gene located on the X chromosome. Loss-of-function mutations in SAP cause the X-linked lymphoproliferative disease （XLP）, a severe immunodeficiency characterized by heightened susceptibility to Epstein-Barr vi- rus and impaired humoral immunity. Normal individuals express several functional and non-functional isoforms of SAP as a result of alternative splicing. In this study, we identify a cryptic exon in the murine Sh2dla gene. At the mRNA level, the new isoform of SAP （SAP-2） that includes this new exon is widely expressed in lymphoid tissues by C57BL/6 and 129 strains of inbred mice. SAP-2 accounts for approximately 1%-3% of total SAP transcripts, and it is dynamically regulated during lym- phocyte activation. At the protein level, the SAP-2 isoform is a 144 amino-acid protein. Compared to the dominant 126 ami- no-acid SAP-I isoform, the additional 18 amino acids are inserted into a structural region that is critical for phosphotyrosine binding. Our functional analysis in vitro indicates that SAP-2 is a non-functional isoform due to decreased protein stability. Thus, both human and mouse have multiple SAP splice isoforms that may or may not function. Modulation of relative propor- tions of these isoforms is potentially a mechanism whereby cells can regulate SAP-mediated biological activities.

关键词： Sh2dla SAP X-linked lymphoproliferative disease (XLP) isoform

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phenotypic Tfh development promoted by CXCR5-controlled re-localization and IL-6 from radiation-resistant cells

引用

Protein & Cell 2015年第11期6卷 825-832页

作者： Xin Chen Weiwei Ma Tingxin Zhang LongyanWu Hai Qi Tsinghua-Peking Center for Life Sciences Tsinghua University Beijing 100084 China laboratory of dynamic immunobiology Institute for Immunology School of Medicine Tsinghua University Beijing 100084 China

How follicular T-helper （Tfh） cells develop is incom- pletely understood. We find that, upon antigen exposure in vivo, both naive and antigen-experienced T cells sequentially upregulate CXCR5 and Bc16 within the first 24 h, relocate to the T-B border, and give rise to phenotypic Bcl6＋CXCR5＋ Tfh cells before the first cell division. CXCR5 upregulation is more dependent on ICOS costimulation than that of Bcl6, and early Bcl6 induction requires T-cell expression of CXCR5 and, presumably, relocation toward the follicle. This early and rapid upregulation of CXCR5 and Bcl6 depends on IL-6 produced by radiation-resistant cells. These results suggest that a Bcl6hiCXCR5hi phenotype does not automatically define a Tfh lineage but might reflect a state of antigen exposure and non-commitment to terminal effector fates and that niches in the T-B border and/or the follicle are important for optimal Bcl6 induction and maintenance.

关键词： Tfh CXCR5 Bcl6 radiation-resistant cell,IL-6

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

T-B entanglement and ICOSL-controlled feed-forward regulation of germinal center reaction

T-B entanglement and ICOSL-controlled feed-forward regulatio...

引用

第九届全国免疫学学术大会

作者： Dan Liu Heping Xu Changming Shih Zurong Wan Xiaopeng Ma Weiwei Ma Dan Luo Hai Qi laboratory of dynamic immunobiology School of MedicineTsinghua University

The germinal center(GC)reaction supports affinity-based B cell competition and generates high-affinity bone-marrow plasmacells(BMPC).How follicular T-helper(T)cells regulate GC selection is not *** competitive mixed chimera,we show that,beyond the role in promoting Tdevelopment,ICOSL is important for individual B cells to competitively participate in the GC reaction and to develop into *** imaging aided by a calcium reporter reveals that ICOSL promotes an"entangled"mode of T-B cell interactions,characterized by brief but extensive surface engagement,productive Tcell calcium spikes,and B-cell acquisition of CD40 *** entanglement promotes outer-zone co-localization of outcompeting GC B cells together with Tcells,affording the former with increased access to T cell *** on GC B cells is upregulated by CD40 *** an intercellular positive feedback between contact-dependent help and ICOSL-controlled entanglement promotes the positive selection and BMPC development,as evidenced by observations that higher-affinity BCR variants are preferentially rendered ICOSL,that numerically disadvantaged ICOSL-deficient GC B cells or BMPCs exhibit strong affinity compensation in competitive chimera,and that when GC competition proceeds without ICOSL,selection of high-affinity variants in otherwise normal GC reaction is *** demonstrating entanglement as the basic form of GC T-B cell interactions,by identifying ICOSL as a molecular linkage between T-B interactional dynamics and positive selection for high-affinity BMPC formation,our study reveals a pathway by which Tcells control the quality of humoral memory.

关键词： Germinal center ICOSL T-B entanglement Affinity

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Transmembrane domain-mediated Lck association underlies bystander and costimulatory ICOS signaling

引用

Cellular & Molecular Immunology 2020年第2期17卷 143-152页

作者： Zurong Wan Xingxing Shao Xingyu Ji Lihui Dong Jiacheng Wei Zhuqing Xiong Wanli Liu Hai Qi Tsinghua-Peking Center for Life Sciences Tsinghua University100084 BeijingChina laboratory of dynamic immunobiology Institute for Immunology100084 BeijingChina Department of Basic Medical Sciences School of Medicine100084 BeijingChina School of Life Sciences 100084 BeijingChina Beijing Key Lab for Immunological Research on Chronic Diseases 100084 BeijingChina MOE Key laboratory of Protein Sciences Tsinghua University100084 BeijingChina

The B7-family inducible costimulator(ICOS)activates phosphoinositide-3 kinase(PI3K)and augments calcium mobilization triggered by the T-cell receptor(TCR).We surprisingly found that the entire cytoplasmic domain of ICOS is dispensable for its costimulation of calcium *** costimulatory function relies on the unique transmembrane domain(TMD)of ICOS,which promotes association with the tyrosine kinase ***-enabled Lck association is also required for p85 recruitment to ICOS and subsequent PI3K activation,and Lck underlies both the bystander and costimulatory signaling activity of ***-replaced ICOS,even with an intact cytoplasmic domain,fails to support T FH development or GC formation in *** transplanted onto a chimeric antigen receptor(CAR),the ICOS TMD enhances interactions between T cells and antigen-presenting target ***,by revealing an unexpected function of the ICOS TMD,our study offers a new perspective for the understanding and potential application of costimulation biology.

关键词： ICOS stimulation stimulator

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroimmunology:reviews and perspectives on recent advances

引用

Cellular & Molecular Immunology 2023年第11期20卷 1257-1258页

作者： Hai Qi Tsinghua-Peking Center for Life Sciences Beijing100084China laboratory of dynamic immunobiology Institute for ImmunologyTsinghua UniversityBeijing100084China Department of Basic Medical Sciences School of MedicineTsinghua UniversityBeijing100084China Changping laboratory Yard 28Science Park Rd.Changping DistrictBeijing102206China New Cornerstone Science laboratory School of MedicineTsinghua UniversityBeijing100084China Beijing Frontier Research Center for Biological Structure Tsinghua UniversityBeijing100084China Beijing Key laboratory for Immunological Research on Chronic Diseases Tsinghua UniversityBeijing100084China SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine School of MedicineTsinghua UniversityBeijing100084China

In an abstract sense,the immune system is tasked to monitor and interpret insults and potential threats from the external world and mount appropriate defensive actions *** the same time,it also monitors states of inte... 详细信息

关键词： perturbation mount system

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microenvironmental influences on T cell immunity in cancer and inflammation

引用

Cellular & Molecular Immunology 2022年第3期19卷 316-326页

作者： Darren R.Heintzman Emilie L.Fisher Jeffrey C.Rathmell Department of Pathology Microbiologyand ImmunologyVanderbilt University Medical CenterNashvilleTN37205USA Vanderbilt Center for immunobiology Vanderbilt University Medical CenterNashvilleTN37205USA

T cell metabolism is dynamic and highly *** the intrinsic metabolic programs of T cell subsets are integral to their distinct differentiation and functional patterns,the ability of cells to acquire nutrients and cope with hostile microenvironments can limit these pathways.T cells must function in a wide variety of tissue settings,and how T cells interpret these signals to maintain an appropriate metabolic program for their demands or if metabolic mechanisms of immune suppression restrain immunity is an area of growing *** in inflamed and cancer tissues,a wide range of changes in physical conditions and nutrient availability are now acknowledged to shape *** include fever and increased temperatures,depletion of critical micro and macro-nutrients,and accumulation of inhibitory waste *** we review several of these factors and how the tissue microenvironment both shapes and constrains immunity.

关键词： immunometabolism T cell microenvironment cancer inflammation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

m6A mRNA methylation controls T cell homeostasis by targeting IL-7/STAT5/SOCS pathway

m6A mRNA methylation controls T cell homeostasis by targetin...

引用

第十二届全国免疫学学术大会

作者： Li Huabing Tong Jiyu Shu Zhu Yuval Kluger Matthew D.Simon Howard Y.Chang Zhinan Yin Richard A.Flavell Department of immunobiology Yale University School of Medicine Shanghai Institute of Immunology Department of Microbiology and Immunology The First Affiliated Hospital Biomedical Translational Research Institute and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering Department of Pathology Yale University School of Medicine Department of Molecular Biophysics & Biochemistry Yale University dynamic Regulomes Stanford University Howard Hughes Medical Institute

N6-methyladenosine(m6A)is the most common and abundant messenger RNA modification,modulated by‘writers’‘erasers’and‘readers’of this *** vitro data have shown that m6A influences all fundamental aspects of mRNA m... 详细信息

关键词： m6A methylation T cells STAT signaling Inflammation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Revisiting the role of mesenchymal stem cells in tuberculosis and other infectious diseases

引用

Cellular & Molecular Immunology 2023年第6期20卷 600-612页

作者： Annu Devi Isha Pahuja Shashi Prakash Singh Akanksha Verma Debapriya Bhattacharya Ashima Bhaskar Ved Prakash Dwivedi Gobardhan Das Special Centre for Molecular Medicine Jawaharlal Nehru UniversityNew DelhiIndia immunobiology Group International Centre for Genetic Engineering and Biotechnology New DelhiIndia Department of Molecular Medicine Jamia Hamdard UniversityNew DelhiIndia Centre for Biotechnology Siksha O Anusandhan UniversityBhubneshwarOdishaIndia

Mesenchymal stem cells(MsCs)play diverse roles ranging from regeneration and wound healing to immune *** investigations have indicated the crucial role of these multipotent stem cells in regulating various aspects of the immune *** express unique signaling molecules and secrete various soluble factors that play critical roles in modulating and shaping immune responses,and in some other cases,MSCs can also exert direct antimicrobial effects,thereby helping with the eradication of invading ***,it has been demonstrated that MSCs are recruited at the periphery of the granuloma containing Mycobacterium tuberculosis and exert"Janus"-like functions by harboring pathogens and mediating host protective immune *** leads to the establishment of a dynamic balance between the host and the *** function through various immunomodulatory factors such as nitric oxide(NO),IDO,and immunosuppressive ***,our group has shown that *** uses MSCs as a niche to evade host protective immune surveillance mechanisms and establish *** also express a large number of ABC efflux pumps;therefore,dormant *** residing in MSCs are exposed to a suboptimal dose of ***,it is highly likely that drug resistance is coupled with dormancy and originates within *** this review,we discussed various immunomodulatory properties of MsCs,their interactions with important immune cells,and soluble *** also discussed the possible roles of MsCs in the outcome of multiple infections and in shaping the immune system,which may provide insight into therapeutic approaches using these cells in different infection models.

关键词： Mesenchymal Stem Cells(MsCs) Macrophages Tuberculosis Infectious Diseases T cells Immunomodulation Immunotherapy.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：