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Reagent-free determination of amikacin content in amikacin sulfate injections by FTIR derivative spectroscopy in a continuous flow system

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Journal of Pharmaceutical Analysis 2014年第2期4卷 125-131页

作者： José F.Ovalles Máximo Gallignani María R.Brunetto Rebeca A.Rondón Carlos Ayala departamento de Análisis y Control Facultad de Farmacia y BioanálisisUniversidad de Los AndesMérida 5101AVenezuela laboratorio de Espectroscopia Molecular Departamento de QuímicaFacultad de CienciasUniversidad de Los AndesMérida 5101AVenezuela

The quantitative estimation of amikacin （AMK） in AMK sulfate injection samples is reported using FTIR-derivative spectrometric method in a continuous flow system. Fourier transform of mid-IR spectra were recorded without any sample pretreatment. A good linear calibration （r40.999, %RSdo 2.0） in the range of 7.7-77.0 mg/mL was found. The results showed a good correlation with the manufacturer＇s and overall they all fell within acceptable limits of most pharmacopoeial monographs on AMK sulfate.

关键词： Amikacin FlrIR derivative spectro-metry Continuous flow system Pharmaceuticalpreparation Injection Sulfate

Morphological and biochemical effects of weekend alcohol consumption in rats: Role of concentration and gender

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World Journal of Hepatology 2018年第2期10卷 297-307页

作者： José A Morales-González María de Lourdes Sernas-Morales ángel Morales-González Laura Ligía González-López Eduardo Osiris Madrigal-Santillán Nancy Vargas-Mendoza Tomás Alejandro Fregoso-Aguilar Liliana Anguiano-Robledo Eduardo Madrigal-Bujaidar Isela álvarez-González Germán Chamorro-Cevallos laboratorio de Medicina de Conservación Escuela Superior de Medicina Instituto Politécnico Nacional Escuela Superior de Cómputo Instituto Politécnico Nacional área Académica de Nutrición ICSa Universidad Autónoma del Estado de Hidalgo depto. de Fisiología Laboratorio de Hormonas y Conducta ENCB campus Zacatenco Instituto Politécnico Nacional laboratorio de Farmacología Molecular Sección de Estudios de Posgrado e Investigación Escuela Superior de Medicina-Instituto Politécnico Nacional laboratorio de Genética Escuela Nacional de Ciencias Biológicas IPN departamento de farmacia Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional

AIM To examine the association between weekend alcohol consumption and the biochemical and histological alterations at two different concentrations of alcohol in both genders in *** Wistar rats weighing 170-200 g were divided into groups as follows:(1) Control groups; and(2) weekend alcohol-consumption group: 2 d/weekly per 12 wk, at two different concentrations:(1) Group of males or females with a consumption of a solution of alcohol at 40%; and(2) group of males or females with a consumption of a solution of alcohol at 5%. At the end of the experiment, serum and liver samples were obtained. The following enzymes and metabolites were determined in serum: Alanine Aminotransferase(ALT), Aspartate Aminotransferase(AST), Lactate dehydrogenase, and Gamma-Glutamyltransferase, and glucose, triglycerides, cholesterol, bilirubin, and albumin. Liver samples from each group were employed to analyze morphological abnormalities by light *** In all of the weekend alcohol-consumption groups, AST activity presented a significant, 10-fold rise. Regarding ALT activity, the groups with weekend alcohol consumption presented a significant increase that was six times greater. Bilirubin levels increased significantly in both groups of females. We observed a significant increase in the parameters of fatty change and inflammation due to weekend alcohol consumption. Only the group of females that consumed alcohol at 40% presented slight hepatocel ular disorganization CONCLUSION The results obtained herein provide solid evidence that weekend alcohol consumption gives rise to liver damage, demonstrated by biochemical and histological alterations, first manifested acutely, and prolonged weekend alcohol consumption can cause greater, irreversible damage.

关键词： Weekend alcohol consumption Liver morphology Transaminases Damage

Nanoencapsulation of antioxidant peptides from Lupinus mutabilis in chitosan nanoparticles obtained by ionic gelling and spray freeze drying intended for colonic delivery

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Food Bioscience 2022年第6期50卷 603-613页

作者： Arturo Intiquilla Karim Jiménez-Aliaga Amparo Iris Zavaleta Alexander Gamboa Nelson Caro Mario Diaz Martin Gotteland Lilian Abugoch Cristian Tapia laboratorio de Biología Molecular Facultad de Farmacia y BioquímicaUniversidad Nacional Mayor de San MarcosJirón Puno1002Lima 1Peru departamento de Ciencia de los Alimentos y Tecnología Química Facultad de Ciencias Químicas y FarmacéuticasUniversidad de ChileSantos Dumont 964SantiagoChile Centro de investigación Austral Biotech Universidad de Santo TomasSantiago de ChileChile Facultad de Odontología Universidad de ChileSantiagoChile departamento de Nutrición Facultad de MedicinaUniversidad de ChileChile.y Laboratorio de Microbiología y ProbióticosInstituto de Nutrición y Tecnología de Alimentos (INTA)Universidad de ChileChile y Núcleo Milenio en la Biología de la Microbiota IntestinalSantiagoChile

Oxidative stress has long been associated with the pathogenesis of inflammatory bowel disease(IBD),and it has been suggested that the combined administration of antioxidants and anti-inflammatory agents may be helpful for its *** from plant proteins,such as soybean glycinin tripeptide VPY,effectively inhibit pro-inflammatory mediators in intestinal epithelial and immune cells,which are both involved in the pathogenesis of *** study aimed to produce antioxidant biopeptides from proteins of Lupinus mutabilis seeds and then encapsulate them in chitosan nanoparticles(NPs)for colonic *** antioxidant peptide fraction of less than three kDa(UF3)was obtained and added at different concentrations(0.1–0.4 mg/ml)in chitosan *** NPs were prepared by gelation with tripolyphosphate(CTPP-UF3)or spray freeze-drying of the chitosan solution(SFDC-UF3).Sizes of 332±13 and 465±58 nm and maximum encapsulation efficiencies of 63.80 and 71.75%,respectively,were ***3 maintained its antioxidant capacity(>80%)and showed different release profiles in 1X PBS buffer at pH 7.4,depending on the encapsulation ***-IR showed hydrogen bonding and electrostatic interactions between the peptide and *** nanosystems maintained cell viability greater than 70%in colonic cell lines *** results show that both methods are appropriate for the nanoencapsulation of UF3 and can be used to design nanoparticles for colonic delivery.

关键词： Antioxidant Peptidic fraction Lupinus mutabilis,chitosan nanoparticles

Rationally designed synthesis of bright AgInS2/ZnS quantum dots with emission control

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Nano Research 2020年第9期13卷 2438-2450页

作者： Jose X.Soares K.David Wegner David S.M.Ribeiro Armindo Melo Ines Hausler Joao L.M.Santos Ute Resch-Genger LAQV-REQUIMTE Department of Chemical SciencesLaboratory of Applied ChemistryFaculty of PharmacyUniversity of PortoRua de Jorge Viterboferreira228Porto4050-313Portugal Federal Institute for Materials Research and Testing(BAM) Division BiophotonicsRichard-Willstaetter-Strasse 1112489 BerlinGermany LAQV-REQUIMTE Laboratorio de Bromatologia e HidrologiaDepartamento de Ciencias QutmicasFaculdade de Farmacia da Universidade do PortoRua de Jorge Viterboferreira228Porto4050-313Portugal Technische Universitat Berlin Institutfiir Optik und Atomare PhysikStrafie des 17.Juni 13510623 BerlinGermany

In the blossoming field of Cd-free semiconductor quantum dots(QDs),ternary Ⅰ-Ⅲ-VI QDs have received increasing attention due to the ease of the environmentally friendly synthesis of high-quality materials in water,their high photoluminescence(PL)quantum yields(QYs)in the red and near infrared(NIR)region,and their inherently low ***,their oxygen-insensitive long PL lifetimes of up to several hundreds of nanoseconds close a gap for applications exploiting the compound-specific parameter PL *** overcome the lack of reproducible synthetic methodologies and to enable a design-based control of their PL properties,we assessed and modelled the synthesis of high-quality MPA-capped AglnS2/ZnS(AlS/ZnS)*** refined parameters included reaction time,temperature,Ag:In ratio,S:In ratio,Zn:In ratio,MPA:ln ratio,and pH using a design-of-experiment *** for the optimization was provided by mathematical models developed for the application-relevant PL parameters,maximum PL wavelength,QY,and PL lifetime as well as the elemental composition in terms of Ag:ln:Zn *** these experimental data-based models,MPA:ln and Ag:ln ratios and pH values were identified as the most important synthesis parameters for PL control and an insight into the connection of these parameters could be ***,the experimental conditions to synthetize QDs with tunable emission and high QY were *** excellent agreement between the predicted and experimentally found PL features confirmed the reliability of our methodology for the rational design of high quality AlS/ZnS QDs with defined PL *** approach can be straightforwardly extended to other ternary and quaternary QDs and to doped QDs.

关键词： quantum dot core/shell nanoparticle silver indium sulfide microw ave-assisted synthesis design of experim ent photoluminescence

A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata

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BIOCELL 2012年第2期36卷 47-55页

作者： ISRAEL A.VEGA FEdeRICO A.deLLAGNOLA JORGE A.HURST MARTÍN S.GOdoY ALFREdo CASTRO-VAZQUEZ laboratorio de Fisiologia(IHEM-CONICET) and Departamento de Morfología y Fisiología(Facultad de Ciencias MédicasUniversidad Nacional de Cuyo)Casilla de Correo 335500 MendozaArgentina Instituto de Ciencias Básicas-Universidad Nacional de Cuyo.Padre Jorge Contreras 1300.Parque Gral.San Martín 5500 Mendoza departamento de Química Biológica.Facultad de farmacia y Bioquímica Universidad de Buenos Aires.Present address:Instituto de Botánica Darwinion.Labardén 200B1642HYD Buenos Aires

Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were *** extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of *** fractions obtained from silica gel column chromatography of the acetone extracts(CI,CII and CIII),were studied by positive ion fast atom bombardment-mass spectrometry(FAB–MS)and hydrogen-nuclear magnetic resonance(H-NMR).Results indicated the presence of(1)a sterol in the yellow colored CI fraction;(2)a mixture of pheophorbides a and b in the major green fraction,CII;and(3)a modified pheophorbide a in the smaller green fraction,*** extracts of the C endosymbiont did not show evidence of the occurrence of C-phycocyanin,allophycocyanin or phycoerithrin(light absorption,fluorescence emission,and electrophoresis of the protein moieties)while cyanobacterial cells(Nostoc sp.)showed evidence of C-phycocyanin and *** possible phylogenetic and functional significance of the pigments present in the C endosymbiont is discussed.

关键词： symbiosis Cyanobacteria chloroplast photosynthetic pigments phylogeny

Structural effects induced by dialysis-based purification of carbon nanomaterials

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Nano Materials Science 2024年第4期6卷 475-483页

作者： A.D.Veloso A.M.Ferraria A.M.Botelho do Rego A.S.Viana A.J.S.Fernandes A.J.Fielding R.A.Videira M.C.Oliveira CQ-VR and Chemistry department University of Tras-os-Montes e Alto Douro5000-801Vila RealPortugal BSIRG iBB-Institute for Bioengineering and BiosciencesDepartment of Chemical Engineeringand Associate Laboratory i4HB-Institute for Health and BioeconomyInstituto Superior TecnicoUniversidade de Lisboa1049-001LisbonPortugal Centro de Química Estrutural Institute of Molecular SciencesDepartamento de Química e BioquímicaFaculdade de Ci^enciasUniversidade de LisboaCampo Grande1749-016LisboaPortugal I3N and Physics department University of AveiroCampus de Santiago3810-193AveiroPortugal Faculty of Science Pharmacy and Biomolecular SchoolLiverpool John Moores UniversityUnited Kingdom REQUIMTE/LAQV Laboratorio de FarmacognosiaDepartamento de QuímicaFaculdade de FarmaciaUniversidade do Porto4050-313PortoPortugal

Dialysis plays a crucial role in the purification of nanomaterials but its impact on the structural properties of carbon nanomaterials was never ***,a carbon-based nanomaterial generated electrochemically in potassium phosphate buffer,was characterized before and after dialysis against pure *** is shown that dialysis affects the size of the carbon domains,structural organization,surface functionalization,oxidation degree of carbon,and grade of ***,dialysis drives the nanomaterial organization from discrete roundish carbon domains,with sizes ranging from 70 to 160 nm,towards linear stacking structures of small nanoparticles(de)surface groups evolve into more oxidized carbon groups(e.g.,ketone and ester groups).Investigation of the as-prepared nanomaterial by electron paramagnetic resonance(EPR)revealed a resonance signal consistent with carbon-oxygen centred ***,this study brings to light the selective affinity of the carbon nanomaterial under study to capture Na^(+)ions,a property greatly enhanced by the dialysis process,and its high ability to trap oxygen,particularly before *** findings open new perspectives for the application of carbon-based nanomaterials and raise awareness of the importance of structural changes that can occur during the purification of carbon-based nanomaterials.

关键词： Nanocarbon Surface functionalization NMR XPS Hydrophilic carbon Paramagnetic

Nanoencapsulation of Human Adipose Mesenchymal Stem Cells: Experimental Factors Role to Successfully Preserve Viability and Functionality of Cells

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Journal of Encapsulation and Adsorption Sciences 2013年第1期3卷 1-12页

作者： Daniel Hachim Jaime Melendez Roberto Ebensperger laboratorio de Terapia Celular y Medicina Regenerativa Departamento de Farmacia Facultad de Química Pontificia Universidad Católica de Chile Santiago Chile

Cell nanoencapsulation is a novel delivery system based on a self-assembly technique mediated by electrostatic interactions called Layer-by-Layer (LbL) deposition, without an increase in volume implant because of the nanometric thickness of its layers. LbL coats the entire surface of individual cells, providing mechanical resistance to cells against manipulation and storage conditions prior to implantation in the patient. In this work, single-cell nanocapsule formation using human adipose-derived mesenchymal stem cells (ADSC) given their potentiality in regenerative medicine was assessed by fluorescence microscopy and Zeta potential assays. Both methodologies were conclusive in showing layer-by-layer nanocapsule formation of every single ADSC. Significant differences in terms of viability and cell functionality preservation were observed depending on the polycation used. Using a combination of fluorescence microscopy and fluorimetric assays, we found that cell survival after nanocapsulation was only efficient when chitosan was added to cells. These results were consistent with other cell types used in this study. Other polycations such as poly(allylamine hydrochloride) (PAH), poly(diallyldimethylammonium chloride) (PDADMAC) and poly-L-lysine (PLL) markedly decreased cell viability (22%, 11% and 15%, respectively). In addition, the use of potassium-enriched saline solutions, such as Hanks and Ringer’s solution, during the nanoencapsulation process on ADSCs was harmful on cell viability compared to standard media (36% vs 79%, respectively). The addition of a mixture of polyanions such as hyaluronic acid and chondroitin sulfate did not affect cell viability (79% and 81%). The combination of chitosan/hyaluronic acid and chondroitin sulfate was also effective in preserving the cell functionality of ADSCs, including the proliferation and differentiation of these cells as assessed by MTT assay and microscopy, respectively. Taken together, these results indicate that ADSCs

关键词： Mesenchymal Stem Cells Nanoencapsulation Polyelectrolytes Chitosan

Evaluation of Antinociceptive and Anti-Inflammatory Effects of Synthetic O-Prenylated Phenolic derivatives

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Pharmacology & Pharmacy 2012年第3期3卷 348-357页

作者： Niraldo Paulino Amarilis Scremin Paulino Priscila Vautier Laura Pisco Carolina Passarelli Jose Matheus de Freitas Costa Dirk Michalik Paulo Celso Pardi Jose Agustín Quincoces Suarez Grupo de pesquisa e desenvolvimento de Biomedicamentos(BIOMED) Programa de Mestrado Profissional em FarmaciaUniversidade Bandeirante de Sao PauloSao PauloBrasil Programa de Pos-Graduacao em farmacia Universidade Federal de Santa CatarinaFlorianopolisBrasil laboratorio de Síntese Organica Universidade Bandeirante de Sao PauloSao PauloBrasil Leibniz-Institut für Katalyse an der Universitat Rostock RostockGermany

This work describes the synthesis, antinociceptive and anti-inflammatory effect of O-prenyl derivatives: O-prenyl derivatives 2-(3-methylbut-2-enyloxy)acetophenone (L1) and 3-methoxy-4-(3-methylbut-2-enyloxy)benzaldehyde (V1). Treatment with L1- or V1-produced antinociceptive effect on classical pain models: acetic acid abdominal contortions and formalin test (first and second phases), and in hot plate or tale flick models in mice without changing the locomotors performance, and anti-oedematogenic or anti-inflammatory effect in vivo: carrageenan-induced paw oedema and peritonitis in mice. In addition, L1 and V1 derivatives reduced nitric oxide production on RAW 264.7 cells stimulated with lipopolysaccharide without changing the cell viability. Taken together, our results show for the first time that L1 and V1 can produce antinociception and modulate inflammatory response when administered in mice.

关键词： Anti-Inflammatory Antinociception Synthesis O-Prenyl Compounds

Review of natural products with hepatoprotective effects

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World Journal of Gastroenterology 2014年第40期20卷 14787-14804页

作者： Eduardo Madrigal-Santillán Eduardo Madrigal-Bujaidar Isela álvarez-González María Teresa Sumaya-Martínez José Gutiérrez-Salinas Mirandeli Bautista ángel Morales-González Manuel García-Luna y González-Rubio J Leopoldo Aguilar-Faisal José A Morales-González laboratorio Medicina de Conservación Escuela Superior de Medicina Instituto Politécnico Nacional Plan de San Luis y Díaz Mirón Col. Casco de Santo Tomás Del. Miguel Hidalgo México DF 11340 México laboratorio de Genética Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional Unidad profesional Adolfo López Mateos Col. Lindavista Delegación Gustavo A. Madero México DF 07738 México Secretaria de Investigación y Estudios de Posgrado Universidad Autónoma de Nayarit "Ciu-dad de la Cultura Amado Nervo" Nayarit 28000 México laboratorio de Bioquímica y Medicina Experimental División de Investigación Biomédica Centro Médico Nacional "20 de Noviembre" ISSSTE México DF 03100 México área Académica de farmacia Instituto de Ciencias de la Salud UAEH Pachuca Hidalgo 42000 México Escuela Superior de Cómputo Instituto Politécnico Nacional Av. Juan de Dios Bátiz s/n esquina Miguel Othón de Mendizabal Unidad Profesional Adolfo López Mateos México DF 07738 Mexico Facultad de Odontología UNAM México DF 04510 Mexico

The liver is one of the most important organs in the body,performing a fundamental role in the regulationof diverse processes,among which the metabolism,secretion,storage,and detoxification of endogenous and exogenous substances are *** to these functions,hepatic diseases continue to be among the main threats to public health,and they remain problems throughout the *** enormous advances in modern medicine,there are no completely effective drugs that stimulate hepatic function,that offer complete protection of the organ,or that help to regenerate hepatic ***,it is necessary to identify pharmaceutical alternatives for the treatment of liver diseases,with the aim of these alternatives being more effective and less *** use of some plants and the consumption of different fruits have played basic roles in human health care,and diverse scientific investigations have indicated that,in those plants and fruits so identified,their beneficial effects can be attributed to the presence of chemical compounds that are called *** present review had as its objective the collecting of data based on research conducted into some fruits(grapefruit,cranberries,and grapes)and plants[cactus pear(nopal)and cactus pear fruit,chamomile,silymarin,and spirulina],which are consumed frequently by humans and which have demonstrated hepatoprotective capacity,as well as an analysis of a resin(propolis)and some phytochemicals extracted from fruits,plants,yeasts,and algae,which have been evaluated in different models of hepatotoxicity.

关键词： Hepatoprotection Grapefruit Vaccinium spp Vitis vinifera L Opuntia ficus-indica Spirulina Propolis Glucans Alcoholic liver disease