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Neural Regeneration Research 2010年第18期5卷 1408-1412页

作者： Huanmin Gao Ling Wang Yunliang Guo department of Neurology Second Affiliated Hospital Qingdao University Medical College Qingdao 266042 Shandong Province China Shandong Province Key Laboratory of Brain Disease Prevention and Treatment Institute of Cerebral Vascular Diseases of Qingdao University Medical College Qingdao 266003 Shandong Province China

Acupuncture has recently gained popularity in many countries as an alternative and complementary therapeutic intervention. Previous studies have shown that changes in genes, proteins, and their metabolites were measureable during acupuncture for treatment of cerebral ischemia. Through the use of in situ hybridization and immunohistochemistry, the present study confirmed that electroacupuncture increased insulin-like growth factor-1 mRNA and protein expression in the corpus stfiatum following cerebral ischemia, reduced brain edema following middle cerebral artery occlusion reperfusion, and decreased infarct volume. Results suggested that electroacupuncture is effective in the relief of cerebral ischemia by increasing endogenous insulin-like growth factor-1 expression.

关键词： electroacupuncture cerebral ischemia focal insulin-like growth factor-1 brain injury neural regeneration

Creutzfeldt-Jakob disease presenting as Korsakoff syndrome caused by E196A mutation in PRNP gene:A case report

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世界临床病例杂志 2023年第25期11卷 5982-5987页

作者： Yong-Kang Zhang Jia-Rui Liu Kang-Li Yin Yuan Zong Yu-Zhen Wang Ye-Min Cao Diagnosis and Treatment Center of vascular Disease Shanghai TCM-Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China

BACKGROUND Prion diseases are a group of degenerative nerve diseases that are caused by infectious prion proteins or gene *** humans,prion diseases result from mutations in the prion protein gene(PRNP).Only a limited number of cases involving a specific PRNP mutation at codon 196(E196A)have been *** coexistence of Korsakoff syndrome in patients with Creutzfeldt-Jakob disease(CJD)caused by E196A mutation has not been documented in the existing *** SUMMARY A 61-year-old Chinese man initially presented with Korsakoff syndrome,followed by rapid-onset dementia,visual hallucinations,akinetic mutism,myoclonus,and *** patient had no significant personal or familial medical *** resonance imaging of the brain revealed extensive hyperintense signals in the cortex,while positron emission tomography/computed tomography showed a diffuse reduction in cerebral cortex *** biochemical and microorganism testing of the cerebrospinal fluid(CSF)yielded normal *** for thyroid function,human immunodeficiency virus,syphilis,vitamin B1 and B12 levels,and autoimmune rheumatic disorders were *** and CSF tests for autoimmune encephalitis and autoantibody-associated paraneoplastic syndrome yielded negative results.A test for 14-3-3 protein in the CSF yielded negative ***-genome sequencing revealed a diseasecausing mutation in *** patient succumbed to the illness 11 months after the initial symptom *** Korsakoff syndrome,typically associated with alcohol intoxication,also manifests in CJD *** with CJD along with PRNP E196A mutation may present with Korsakoff syndrome.

关键词： Prion disease Creutzfeldt-Jakob disease Korsakoff syndrome PRNP gene 14-3-3 proteins Case report

Melatoninergic Antidepressant in Post-Stroke depression Therapy

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Advances in Aging Research 2018年第2期7卷 29-38页

作者： Victor V. Kuznetsov department of cerebral vascular Pathology Chebotarev Institute of Gerontology National Academy of Medical Sciences Kiev Ukraine

This paper presents the results of the effects of Melitor (25 mg during two months) on the psychic-emotional state, cerebral hemodynamic and bioelectrical activity of the brain in 30 patients (average age 66.2 ± 3.2 years) with ischemic post-stroke depression. It has been found that this drug possesses a multimodal action which is characterized by the decrease of the level of depression, widening of the range of social-environmental activity, improvement of the cerebral blood circulation and harmonization of the brain electrogenesis. In the patients with right versus left hemisphere stroke, the effect of Melitor on the functional state of the brain was more pronounced. Based on the results obtained in our study, we can recommend Melitor to use it in complex scheme of rehabilitation care of patients of the given nosological category.

关键词： Ischemic Stroke Post-Stroke depression cerebral Hemodynamic Brain Bioelectrical Activity Melitor

deep learning-enabled fast DNA-PAINT imaging in cells

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Biophysics Reports 2023年第4期9卷 177-187页

作者： Min Zhu Luhao Zhang Luhong Jin Yunyue Chen Haixu Yang Baohua Ji Yingke Xu department of Biomedical Engineering Key Laboratory of Biomedical Engineering of Ministry of Education State Key Laboratory of Extreme Photonics and Instrumentation Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine for Clinical Evaluation and Translational Research Zhejiang University Binjiang Institute of Zhejiang University department of Engineering Mechanics Biomechanics and Biomaterials Laboratory Zhejiang University department of Endocrinology Children's Hospital of Zhejiang University School of Medicine National Clinical Research Center for Children's Health

DNA-based point accumulation in nanoscale topography(DNA-PAINT) is a well-established technique for single-molecule localization microscopy(SMLM), enabling resolution of up to a few ***, DNA-PAINT involves the utilization of tens of thousands of single-molecule fluorescent images to generate a single super-resolution image. This process can be time-consuming, which makes it unfeasible for many researchers. Here, we propose a simplified DNA-PAINT labeling method and a deep learning-enabled fast DNA-PAINT imaging strategy for subcellular structures, such as microtubules. By employing our method, super-resolution reconstruction can be achieved with only onetenth of the raw data previously needed, along with the option of acquiring the widefield image. As a result, DNA-PAINT imaging is significantly accelerated, making it more accessible to a wider range of biological researchers.

关键词： DNA-PAINT U-Net SMLM Super-resolution imaging deep learning

Synthetic neurosteroids on brain protection

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Neural Regeneration Research 2015年第1期10卷 17-21页

作者： Mariana Rey Héctor Coirini laboratorio de Neurobiología Instituto de Biología y Medicina Experimental (IBYME-CONICET) Vuelta de Obligado 2490 (C1428ADN) Ciudad Autónoma de Buenos AiresArgentina departamento de Bioquímica Humana Facultad de MedicinaUniversidad de Buenos Aires Paraguay 2155 Ciudad Autónoma de Buenos AiresArgentina

Neurosteroids, like allopregnanolone and pregnanolone, are endogenous regulators of neuronal excitability. Inside the brain, they are highly selective and potent modulators of GABAA receptor activity. Their anticonvulsant, anesthetics and anxiolytic properties are useful for the treatments of several neurological and psychiatric disorders via reducing the risks of side effects obtained with the commercial drugs. The principal disadvantages of endogenous neurosteroids adminis- tration are their rapid metabolism and their low oral bioavailability. Synthetic steroids analogues with major stability or endogenous neurosteroids stimulation synthesis might constitute prom- ising novel strategies for the treatment of several disorders. Numerous studies indicate that the 3ct-hydroxyl configuration is the key for binding and activity, but modifications in the steroid nucleus may emphasize different pharmacophores. So far, several synthetic steroids have been developed with successful neurosteroid-like effects. In this work, we summarize the properties of various synthetic steroids probed in trials throughout the analysis of several neurosteroids-like actions.

关键词： allopregnanolone synthetic steroids GABAA receptor neuroprotection cerebral cortex hippocampus

Impacts of fire severity and cattle grazing on early plant dynamics in old-growth Araucaria-Nothofagus forests

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Forest Ecosystems 2019年第4期6卷 298-311页

作者： Paola Arroyo-Vargas Andres Fuentes-Ramírez Bart Muys Aníbal Pauchard laboratorio de Biometría Departamento de Ciencias ForestalesUniversidad de La FronteraCasilla 54-DTemucoChile Programa de Magíster en Manejo de Recursos Naturales Facultad de Ciencias Agropecuarias y ForestalesUniversidad de La FronteraCasilla 54-DTemucoChile Butamallin Research Center for Global Change Universidad de La FronteraTemucoChile Instituto de Ecología y Biodiversidad(IEB) SantiagoChile department of Earth and Environmental Sciences University of LeuvenCelestijnenlaan 200EBox 24113001 LeuvenBelgium laboratorio de Invasiones Biológicas(LIB) Facultad de Ciencias ForestalesUniversidad de ConcepciónCasilla 160-CConcepciónChile.

Background: Fire has historically shaped the dynamics of Araucaria araucana-Nothofagus pumilio forests in the Andean region of South America. Nevertheless, human disturbances and stronger drought events have resulted in increased occurrence and severity of wildfires. Regardless of their conservation relevance, the initial response to fire of Araucaria-Nothofagus forests has not been well documented. Through this research we tested the hypothesis that plant composition of Araucaria-Nothofagus forest after fire will initially differ in its recovery depending on fire severity, but over time, plant similarity will increase among areas with different levels of fire severity. The study was carried out in old-growth Araucaria-Nothofagus forests in south-central Chile(38°S) that were burned in 2015. We studied vegetation response to three levels of fire severity(low(LS), moderate(MS) and high(HS) severity), also including an adjacent unburned forest(UN), which were assessed one, two and three years after fire. We also evaluated the impact of cattle on plant recovery after fire. We measured species richness, abundance, plant diversity and origin of all vascular plant ***: Species richness and plant abundance responded differently to fire severity. Time-since-fire had a significant effect on plant richness and abundance. Plant composition within LS areas was similar to UN areas, but MS and HS areas were significantly less diverse compared to UN and LS. In absence of other major disturbances, similarity in plant composition tended to increase over time across the different levels of fire severity, becoming more similar to UN. We also found a synergetic effect of cattle and fire on species richness. This interaction promotes the establishment of nonnative species and limits the recovery of native species following ***: Fire severity had a significant impact on the plant community of old-growth Araucaria-Nothofagus forests,especially in areas of high fir

关键词： Fire severity gradient Wildfire Post-fire plant recovery Cattle exclusion Synergistic effects of disturbances Araucaria araucana Nothofagus pumilio

The adjustment of γ-aminobutyric acid_A tonic subunits in Huntington＇s disease：from transcription to translation to synaptic levels into the neostriatum

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Neural Regeneration Research 2018年第4期13卷 584-590页

作者： Abraham Rosas-Arellano Argel Estrada-Mondragón Carola A.Mantellero Carlos Tejeda-Guzmán Maite A.Castro Instituto de Bioquimica y Microbiologia Facultad de Ciencias Universidad Austral de Chile Valdivia Chile Center for Interdisciplinary Studies on the Nervous System (CISNe) Universidad Austral de Chile Valdivia Chile departamento de Fisiologla Biofisica y Neurociencias Cinvestav del IPN Ciudad de Mexico Mexico Queensland Brain Institute The University of Queensland Brisbane Australia laboratorio de Neurociencias Departamento de Biologia Facultad de Quimica y Biologla Universidad de Santiago de Chile Santiago de Chile Chile

γ-Aminobutyric acid（GABA）,plays a key role in all stages of life,also is considered the main inhibitory *** activates two kind of membrane receptors known as GABAA and GABAB,the first one is responsible to render tonic inhibition by pentameric receptors containing α4-6,β3,δ,or ρ1-3 subunits,they are located at perisynaptic and/or in extrasynaptic *** biophysical properties of GABAA tonic inhibition have been related with cellular protection against excitotoxic injury and cell death in presence of excessive *** this basis,GABAA tonic inhibition has been proposed as a potential target for therapeutic intervention of Huntington＇s ***＇s disease is a neurodegenerative disorder caused by a genetic mutation of the huntingtin *** experimental studies of Huntington＇s disease mouse models have been developed,such as R6/1,R6/2,Hdh Q92,Hdh Q150,as well as *** all of them,some key experimental reports are focused on *** neostriatum is considered as the most important connection between cerebral cortex and basal ganglia structures,its cytology display two pathways called direct and indirect constituted by medium sized spiny neurons expressing dopamine D1 and D2 receptors respectively,they display strong expression of many types of GABAA receptors,including tonic *** studies about of GABAA tonic subunits and Huntington＇s disease into the neostriatum are rising in recent years,suggesting interesting changes in their expression and localization which can be used as a strategy to delay the cellular damage caused by the imbalance between excitation and inhibition,a hallmark of Huntington＇s disease.

关键词： GABAA extrasynaptic and perisynaptic y-aminobutyric acidA receptors striatum R6/1, R6/2,HdhQ92, HdhQ111, HdhQ150, N171-82Q and YAC128 HD transgenics mice models chorea mutanthuntingtin inhibitory neurotransmission D1 medium sized spiny neurons D2 medium sized spiny neurons

Comparison of low-dose sequences of dual-source CT and echocardiography for preoperative evaluation of aortic valve disease

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Chinese Medical Journal 2013年第23期126卷 4423-4429页

作者： FENG Juan WANG Xi-ming JI Xiao-peng LI Hai-ou LI Qiao GUO Wen-bin WANG Zheng-jun department of Ultrasound Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong 250021 China department of Cardiovascular Surgery Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong 250021 China Shandong Provincial Key Laboratory of Diagnosis and Treatment of Cardio-cerebral vascular Diseases Shandong Medical Imaging Research Institute Jinan Shandong 250021 China

Background Accurate evaluation of coronary artery,aortic valve annulus diameter （AVAD）,and cardiac function in patients with aortic valve disease is of great significance for surgical *** this study,we explored the preoperative evaluation of low-dose sequence （MinDose sequence） scan of dual-source CT （DSCT） for those *** Forty patients suspected for aortic valve disease （the experimental group） underwent MinDose sequence of DSCT to observe coronary artery,AVAD,and left ventricular ejection fraction （LVEF）.Another 33 subjects suspected for coronary artery disease （the control group） underwent conventional retrospective electrocardiographically-gated sequence of ***-dimensional transthoracic echocardiography （2D-TTE） and four-dimensional transthoracic echocardiography （4D-TTE） were applied in the experimental group to measure AVAD and LVEF and compared with *** There was a strong correlation between LVEFs measured by 2D-TTE and MinDose-DSCT （r=0.87,P ＜0.01）,as well as between 4D-TTE and MinDose-DSCT （r=0.90,P ＜0.01）.AVAD measured by MinDose-DSCT was in good agreement with corresponding measurements by 2D-TTE （r=0.90,P ＜0.01）.The effective dose in the experimental group was 63.54％ lower than that in the control *** MinDose sequence of DSCT with a low radiation dose serving as a one-stop preoperative evaluation makes effective assessment of the coronary artery,AVAD,and LVEF for patients with aortic valve disease.

关键词： radiation dose coronary CT angiography dual-source CT cardiac function aortic valve annulus diameters

Immunometabolic bases of type 2 diabetes in the severity of COVID- 19

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World Journal of Diabetes 2021年第7期12卷 1026-1041页

作者： Rebeca Viurcos-Sanabria Galileo Escobedo Research Division Hospital General de MéxicoMexico City 06720Mexico laboratorio de Proteómica Dirección de InvestigaciónHospital General de Mexico“Dr.Eduardo Liceaga”Mexico City 06720Mexico

The outbreak of coronavirus disease 2019(COVID-19)is caused by the novel severe acute respiratory syndrome coronavirus-2(SARS-CoV-2).COVID-19 and type 2 diabetes(T2D)have now merged into an ongoing global syndemic that is threatening the lives of millions of people around the *** this reason,there is a deep need to understand the immunometabolic bases of the main etiological factors of T2D that affect the severity of ***,we discuss how hyperglycemia contributes to the cytokine storm commonly associated with COVID-19 by stimulating monocytes and macrophages to produce interleukin IL-1β,IL-6,and TNF-αin the airway *** main mechanisms through which hyperglycemia promotes reactive oxygen species release,inhibition of T cell activation,and neutrophil extracellular traps in the lungs of patients with severe SARS-CoV-2 infection are also *** further examine the molecular mechanisms by which proinflammatory cytokines induce insulin resistance,and their deleterious effects on pancreaticβ-cell exhaustion in T2D patients critically ill with *** address the effect of excess glucose on advanced glycation end product(AGE)formation and the role of AGEs in perpetuating pneumonia and acute respiratory distress ***,we discuss the contribution of preexisting endothelial dysfunction secondary to diabetes in the development of neutrophil trafficking,vascular leaking,and thrombotic events in patients with severe SARS-CoV-2 *** we outline here,T2D acts in synergy with SARSCoV-2 infection to increase the progression,severity,and mortality of *** think a better understanding of the T2D-related immunometabolic factors that contribute to exacerbate the severity of COVID-19 will improve our ability to identify patients with high mortality risk and prevent adverse outcomes.

关键词： COVID-19 SARS-CoV-2 Type 2 diabetes Inflammation Hyperglycemia Prothrombotic state

Effects of Danhong Injection(丹红注射液) and Its Main Components on Anticoagulation and Fibrinolysis in Cultured Vein Endothelial Cells

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Chinese Journal of Integrative Medicine 2016年第4期22卷 276-283页

作者：张宇燕周惠芬杨洁红何昱陈小强 Katsuyoshi Nishinari 万浩芳万海同 Research Institution of Cardio-cerebral-vascular Disease Zhejiang University of Traditional Chinese Medicine Pharmaceutical College Zhejiang University of Traditional Chinese Medicine School of Food and Pharmaceutical Engineering Hubei University of Technology

Objective： To observe the effects of Danhong Injection （丹红注射液） and its main components, including daiclzein and hydroxysafflor yellow A （HSYA）, on the anticoagulation, fibrinolysis, anti-apoptosis in hypoxia model of vein endothelial cells （VECs）. Methods： VECs were prepared and were put in a hypoxia environment, which consisted of mixed gas of 95% N2 and 5% CO2 mixed gas, when reached confluent culture. Five groups used different treatments, including normal control group, hypoxia group, daiclzein group, HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection, daiclzein and HSYA on 6 keto prostaglandin F1α （6-keto-PGF1 α） level was measured by the method of radioimmunoassay （RIA）. Superoxide dismutase （SOD） activity was tested by water soluble tetrazolium salt. The content of malondialdehyde （MDA） was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator （t-PA） and plasminogen activator inhibitor （PAl） were measured by the method of chromogenic substrate. The contents of endothelin （ET） and nitric oxide （NO） were detected by non-equilibrium RIA and enzyme- linked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry. Results： Compared with the normal control group, the floating cells number, PAl activity, ET and MDA contents, and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased, whereas the 6-keto-PGF1 α and NO contents, and t-PA and SOD activities were decreased significantly （P〈0.01）. Compared with the hypoxia group, Danhong Injection markedly increased the 6-keto-PGF1 α content and SOD activity, regulated PAl and t-PA activities, ET and NO contents, and decreased MDA content and cells apoptosis rate （P〈0.05 or P〈0.01）. Conclusions： Danhong Injection and its main components played an important role in protecting primary VECs

关键词： vein endothelial cells Danhong Injection daiclzein hydroxysafflor yellow A anticoagulation,flbrinolysis Chinese medicine