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Helicobacter pylori-induced inflammation and epigenetic changes during gastric carcinogenesis

World Journal of Gastroenterology 2015年第45期21卷 12742-12756页

作者： Manuel A Valenzuela Jimena Canales Alejandro H Corvalán Andrew FG Quest laboratorio de comunicaciones celulares Centro de Estudios Moleculares de la Célula Programa de Biología Celular y Molecular ICBM Facultad de Medicina Universidad de Chile Cellular Communication Laboratory Center for Molecular Studies of the Cell Advanced Center for Chronic Diseases School of Medicine Universidad de Chile UC-Centro de Investigación en Oncología Pontificia Universidad Católica de Chile departamento de Hematología-Oncología Facultad de Medicine Pontificia Universidad Católica de Chile

The sequence of events associated with the development of gastric cancer has been described as "the gastric precancerous cascade". This cascade is a dynamic process that includes lesions, such as atrophic gastritis, intestinal metaplasia and dysplasia. According to this model, Helicobacter pylori(H. pylori) infection targets the normal gastric mucosa causing non-atrophic gastritis, an initiating lesion that can be cured by clearing H. pylori with antibiotics or that may then linger in the case of chronic infection and progress to atrophic gastritis. The presence of virulence factors in the infecting H. pylori drives the carcinogenesis process. Independent epidemiological and animal studies have confirmed the sequential progression of these precancerous lesions. Particularly long-term follow-up studies estimated a risk of 0.1% for atrophic gastritis/intestinal metaplasia and 6% in case of dysplasia for the long-term development of gastric cancer. With this in mind, a better understanding of the genetic and epigenetic changes associated with progression of the cascade is critical in determining the risk of gastric cancer associated with H. pylori infection. In this review, we will summarize some of the mostrelevant mechanisms and focus predominantly but not exclusively on the discussion of gene promoter methylation and mi RNAs in this context.

关键词： Helicobacter pylori Methylation Gastric cancer Epi

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Adipose-derived mesenchymal stem/stromal cells:from the lab bench to the basic concepts for clinical translation

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BIOCELL 2018年第3期42卷 67-77页

作者： Yesica Romina FRONTINI-LóPEZ Aldana Daniela GOJANOVICH Diego MASONE Diego Martín BUSTOS Marina UHART laboratorio de Integración de Señles celulares.IHEM-CONICET Universidad Nacional de Cuyo MendozaArgentina Facultad de Ingeniería Universidad Nacional de CuyoMendozaArgentina Facultad de Ciencias Exactas y Naturales Universidad Nacional de CuyoMendozaArgentina

In the last years,much work has shown that the most effective repair system of the body is represented by stem cells,which are defined as undifferentiated precursors that own unlimited or prolonged self-renewal ability,which also have the potential to transform themselves into various cell types through *** tissues that form the body contain many different types of somatic cells,along with stem cells that are called‘mesenchymal stem(or stromal)cells’(MSC).In certain circumstances,some of these MSC migrate to injured tissues to replace dead cells or to undergo differentiation to repair *** discovery of MSC has been an important step in regenerative medicine because of their high ***,the finding of a method to isolate MSC from adipose tissue,so called‘adipose-derived mesenchymal stem cells’(ASC),which share similar differentiation capabilities and isolation yield that is greater than other MSC,and less bioethical concerns compared to embryonic stem cells,have created self-praised publicity to procure almost any treatment with ***,we review the current techniques for isolation,culture and differentiation of human ASC(hASC),and describe them in *** also compile some advantages of the hASC over other stem cells,and provide some concepts that could help finding strategies to promote their therapeutic efficiency.

关键词： hASC Tissue regeneration Cell therapy

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Freely Available Tool(FAT)for automated quantification of lipid droplets in stained cells

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BIOCELL 2017年第2期41卷 55-58页

作者： DIEGO MASONE ALDANA DGOJANOVICH YESICA R.FRONTINI-LOPEZ SAMANTA deL VELIZ MARINA UHART DIEGO M.BUSTOS laboratorio de Integración de Senales celulares.IHEM-CONICET Universidad Nacional de Cuyo MendozaArgentina Facultad de Ingeniería Universidad Nacional de CuyoMendozaArgentina Facultad de Ciencias Exactas y Naturales Universidad Nacional de CuyoMendozaArgentina

In this study,we propose an automatic procedure for digital image *** describe a method that can efficiently quantify and characterize lipid droplets distributions in different cell types in ***,the lipid droplets detection method described in this work could be applied to static or time-lapse data,collected with a simple visible light or fluorescence microscopy *** automated algorithms were implemented in Octave,a freely available scientific package.

关键词： Adipogenesis 3T3-L1 cells human stem cells automatic image processing

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E-Cadherin Dysfunction and Cancer

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Journal of Biosciences and Medicines 2019年第8期7卷 42-67页

作者： María Fernanda Izaguirre Carolina Daniela Galetto Lautaro Baró Víctor Hugo Casco laboratorio de Microscopía Aplicada a Estudios Moleculares y celulares Facultad de Ingeniería Universidad Nacional de Entre Ríos Oro Verde Entre Ríos República Argentina Instituto de Investigación y desarrollo en Bioingeniería y Bioinformática (IBB) Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET) CABA República Argentina

E-cadherin molecules are cell-cell molecular connectors, but also act attaching cell surface to the cytoskeleton through catenin’s and additional partner proteins. Its main function is to regulate cellular adhesion and motility, and therefore acts as an invasion suppressor system. Its role is crucial in the induction and maintenance of cell polarity and differentiation, and in the organization and maintenance of tissue architecture. Downregulation or loss of its function is associated with an invasive and aggressive phenotype in many types of human cancers. In alterations of animal development, E-cadherin dysfunction influence in premature lethality or epidermal barrier and immunity defects. However, new hypothesis over its promoter role in tissue invasion is on focus. In addition to its tumor suppressor role, E-cadherin is a guiding molecule in collective cell migration increasing the metastasis risk during in vivo tumorigenesis. This E-cadherin function explains the retention of the functional E-cadherin expression, and that the epithelial-mesenchymal transition (EMT) is not required for the metastasis occurrence. However, like a switch, in some tumoral environments E-cadherin post-translational modifications may cause cell cluster migration. Like in development, in certain in vivo tumoral contexts, E-cadherin apparently involves signaling rather than cell contact formation. In this report, we explore the possibility of a novel role of thyroid hormones (THs) in the signal via E-cadherin-catenins, this not only should be involved in development and homeostasis, but also in cancer susceptibility of gastrointestinal tract.

关键词： Cell Adhesion Molecules Complexes E-Cadherin Cancer Thyroid Hormones

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New Amphiphilic Amino Acid derivatives for Efficient DNA Transfection in Vitro

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Advances in Chemical Engineering and Science 2017年第2期7卷 191-205页

作者： Lucía C.Pena María F.Argaraná María M.de Zan Antonella Giorello Sebastián Antuna Claudio C.Prieto Carolina M.I.Veaute Diana M.Müller LAQUIMAP Dto.Química OrgánicaUniversidad Nacional del LitoralSanta FeArgentina Cát.Microbiología General Universidad Nacional del LitoralSanta FeArgentina Laoratorio de Control de Medicamentos Universidad Nacional del LitoralSanta FeArgentina Instituto de Investigaciones en Catálisis y Petroquímica Universidad Nacional del LitoralSanta FeArgentina laboratorio de Cultivos celulares Universidad Nacional del LitoralSanta FeArgentina laboratorio de Inmunología Básica Universidad Nacional del LitoralSanta FeArgentina

Nucleic acids-based therapies have recently developed as next-generation agents for treating and preventing viral infection, cancer, and genetic disorders, but their use is still limited due to its relatively poor delivery into targeted cells. We designed and synthesized new amphiphilic amino acid derivatives (cysteine-based) of low molecular weight, formed by the same pentapeptide (AG2: WWCOO) N-acylated, with different hydrophobic chains containing from 12 to 18 carbons, named AG2-Cn (N), which dimerize by oxidation in the presence of pLenti-CMV-GFP Puro plasmid (P) in the respective gemini. We determined transfection efficiency, critical micelle concentration, particle size, ζ-potential and cytotoxicity for the derivatives obtained. We found that all the synthesized compounds were active for DNA delivery and had greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is a promising carrier for gene delivery because it showed no cytotoxicity and its activity was greater than or equal to the commercial actives currently used.

关键词： Amphiphile N-Acylated Cysteine Gemini Ornithine Transfection

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