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Spatiotemporal oxygen dynamics in young leaves reveal cyclic hypoxia in plants

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Molecular Plant 2024年第3期17卷 377-394页

作者： Paolo M.Triozzi Luca Brunello Giacomo Novi Gianmarco Ferri Francesco Cardarelli Elena Loreti Mariano Perales Pierdomenico Perata PlantLab Center of Plant SciencesSant'Anna School of Advanced Studies56010 PisaItaly Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid(UPM)-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria(INIA-CSIC)Campus de Montegancedo UPMPozuelo de Alarcon28223 MadridSpain Fondazione Pisana per la Scienza 56017 PisaItaly laboratorio NEST Scuola Normale SuperioreIstituto Nanoscienze-CNRPiazza S.Silvestro1256127 PisaItaly institute of Agricultural Biology and Biotechnology National Research Council56124 PisaItaly Departamento de Biotecnologia-Biologia Vegetal Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de BiosistemasUniversidad Politecnica de Madrid(UPM)28040 Madrid.Spain

Oxygen is essential for plant growth and *** occurs in plants due to limited oxygen avail-abilityfollowing adverse environmental conditions as well inhypoxic niches in otherwisenormoxic ***,the existence and functional integration of spatiotemporal oxygen dynamics with plant development remains *** animal systems dynamic fluctuations in oxygen availability are known as cyclic *** this study,we demonstrate that cyclic fluctuations in internal oxygen levels occur in young emerging leaves of Arabidopsis *** hypoxia in plants is based on a mechanism requiring the ETHYLENE RESPONSE FACTORS type VII(ERFVII)that are central components of the oxygen-sensing machinery in *** ERFVll-dependent mechanism allows precise adjustment of leaf growth in response to carbon status and oxygen availability within plant *** study thus establishes a functional connection between internal spatiotemporal oxygen dynamics and developmental processes of plants.

关键词： Arabidopsis hypoxia cyclic hypoxia leaf development oxygen-sensing mechanism ERFVII

Analysis of fluoxetine-induced plasticity mechanisms as a strategy for understanding plasticity related neural disorders

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Neural Regeneration Research 2016年第4期11卷 547-548页

作者： Francesco Mattia Rossi laboratorio de Neurociencias "Neuroplasticity Unit" Facultad de CienciasUdelaR

Fluoxetine hydrochloride,better known for its commercial name Prozac,is one of the most widely prescribed antidepressant drugs all over the *** drug was considered a＂breakthrough drug＂for the treatment of depression because of its very high selectivity as a serotonin reuptake inhibitor and because it presented a lower side-effectprofile than previous drugs （Wong et al., 2005）.

关键词： Analysis of fluoxetine-induced plasticity mechanisms as a strategy for understanding plasticity related neural disorders

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Curcuma longa L. extract improves the cortical neural connectivity during the aging process

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Neural Regeneration Research 2017年第6期12卷 875-880页

作者： Gonzalo Flores laboratorio de Neuropsiquiatría Instituto de Fisiología Benemérita Universidad Autónoma de Puebla

Turmeric or Curcuma is a natural product that has anti-inflammatory, antioxidant and anti-apoptotic pharmacological properties. It can be used in the control of the aging process that involves oxidative stress, inflammation, and apoptosis. Aging is a physiological process that affects higher cortical and cognitive functions with a reduction in learning and memory, limited judgment and deficits in emotional control and social behavior. Moreover, aging is a major risk factor for the appearance of several disorders such as cerebrovascular disease, diabetes mellitus, and hypertension. At the brain level, the aging process alters the synaptic intercommunication by a reduction in the dendritic arbor as well as the number of the dendritic spine in the pyramidal neurons of the prefrontal cortex, hippocampus and basolateral amygdala, consequently reducing the size of these regions. The present review discusses the synaptic changes caused by the aging process and the neuroprotective role the Curcuma has through its anti-inflammatory, antioxidant and anti-apoptotic actions.

关键词： Curcuma longa cortical curcumin hippocampus synaptic antioxidant spine connectivity cortex

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Implementation of a New Solution for the Preservation of Anatomical Specimens Made of Non-Toxic Substances

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Open Journal of Veterinary Medicine 2024年第3期14卷 56-67页

作者： Ma Reyes Pichardo-Molinero Samantha Jardon-Xicotencatl Misael R. Oliver-González Carlos G. García-Tovar laboratorio de Apoyo Técnico a la Anatomía Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico Unidad de Investigación Multidisciplinaria L4 (Morfología Veterinaria y Biología Celular) Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico

The preservation of anatomical pieces in Veterinary Anatomy is essential since it is not possible to dissect all domestic species. Most techniques use reagents with high levels of toxicity such as formaldehyde. The objective of this work was to develop a new preservation technique that uses reagents with zero toxicity and that allows obtaining preserved pieces suitable for anatomical studies. The alcohol propylene glycol technique was developed, the method of which uses a fixation step with alcohol, sodium chloride, commercial vinegar and subsequently the impregnation of the preservation solution made from propylene glycol and commercial vinegar, which are non-toxic. As a result of this work, adequately preserved sheep hearts were obtained that preserved their morphology with slight changes in size and weight, which did not affect their external and internal anatomical structure. Its coloration was not substantially affected, remaining a little lighter. The pieces obtained showed flexibility which allowed dissections to be carried out. The time to develop the technique was 20 days. A comparative study was carried out with the phenolated glycerin technique that uses toxic reagents (formaldehyde and phenol) and the pieces obtained with the alcohol propylene glycol technique were of better quality, observing that the pieces with phenolated glycerin tend to darken and are more rigid. And the time to develop the technique is 24 days. In conclusion, a preservation technique for anatomical pieces was developed that allowed the preservation of the organs under study, which allow their use for anatomical studies, and which have been preserved without changes until the time of this publication (8 months) and there are pieces preserved with this technique for 2 years.

关键词： Veterinary Anatomy Heart Preservation Techniques Alcohol Propylene Glycol Technique Fixation Necropsy Toxic Chemicals

A multi-shot target wheel assembly for high-repetition-rate, laser-driven proton acceleration

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High Power Laser Science and Engineering 2024年第2期12卷 87-94页

作者： J.Penas A.Bembibre D.Cortina-Gil L.Martin A.Reija C.Ruiz M.Seimetz A.Alejo J.Benlliure Instituto Galego de Fisica de Altas Enerxias Universidade de Santiago de CompostelaSantiago de CompostelaSpain laboratorio Laser de Aceleracion y Aplicaciones RIAIDTUniversidade de Santiago de CompostelaSantiago de CompostelaSpain Instituto Universitario de Física Fundamental y Matematicas y Dpto.de Didactica de la Matematica y de las Ciencias Experimentales Universidad de SalamancaSalamancaSpain Instituto de Instrumentación para Imagen Molecular(I3M) CSIC-Universitat Politecnica de ValenciaValenciaSpain

A multi-shot target assembly and automatic alignment procedure for laser–plasma proton acceleration at high repetition rate are *** assembly is based on a multi-target rotating wheel capable of hosting more than 5000 targets,mounted on a 3D motorized stage to allow rapid replenishment and alignment of the target material between laser *** automatic alignment procedure consists of a detailed mapping of the impact positions at the target surface prior to the irradiation that ensures stable operation of the target,which alongside the purpose-built design of the target wheel,enables operation at rates up to 10 *** and continuous laser-driven proton acceleration at 10 Hz is demonstrated,with observed cut-off energy stability about 15%.

关键词： high repetition rate laser-plasma acceleration multi-shot operation proton source target assembly

A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

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Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 62-70页

作者： Natalia Souza de Godoy Manoel Sebastiao da Costa Lima-Junior Jose Angelo Lauletta Lindoso Vera Lucia Pereira-Chioccola Thelma Suely Okay Lucia Maria Almeida Braz laboratorio de Parasitologia Médica Departamento de Moléstias Infecciosas e Parasitárias do Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil laboratorio de Imunopatologia e Biologia Molecular-Instituto Aggeu Magalhaes/IAM-FIOCRUZ RecifeBrazil laboratorio de Soroepidemiologia e Imunobiologia-FMUSP Sao PauloBrazil laboratorio de Biologia Molecular de Parasitas e Fungos-Instituto Adolfo Lutz Sao PauloBrazil laboratorio de Parasitologia-Instituto de Medicina Tropical de Sao Paulo-USP Sao PauloBrazil

Objective:To determine an algorithm for molecular diagnosis of visceral leishmaniasis(VL)by kinetoplast DNA(kDNA)(RV1/RV2)and internal transcriber spacer(ITS1)(LITSR/L5.8 S)polymerase chain reaction(PCR),complemented by ITS 1 PCR restriction fragment length polymorphism(RFLP),using peripheral blood or bone marrow aspirate from patients with suspected ***:Biological samples were submitted to the gold standard for the diagnosis of VL and molecular diagnosis represented by ITS 1 PCR,kDNA PCR,and ITS 1 PCR *** samples were obtained from seven groups:groupⅠ,82 samples from patients with confirmed VL;groupⅡ,16 samples from patients under treatment for VL;groupⅢ,14 samples from dogs with canine visceral leishmaniasis(CVL);groupⅣ,a pool of six experimentally infected sandflies(Lutzomya longipalpis);group V,18 samples from patients with confirmed tegumentary leishmaniasis(TL)and groupsⅥandⅦwere from control groups without ***:The following gold standard and molecular examination results were obtained for each of the seven groups:groupⅠ:parasitologic and immunochromatographic tests showed a sensitivity of 76.3%(61 of 80)and 68.8%(55 of 80),respectively,and a sensitivity of 97.6%(80 of 82)and 92.7%(76 of 82)by ITS1 and kDNA PCR,*** ITS1 PCR RFLP(HaeⅢ)analysis of the 80 positive samples,52.5%(42 of 80)generated three fragments of 180,70,and 50 bp,corresponding to the pattern of Leishmania infantum infantum;groupⅡ:negative for the parasitologic methods and positive for IrK39(100%,16 of 16),presented 12.5%(2 of 16)of positivity by ITS 1 PCR and 25.0%(4 of 16)by kDNA PCR;groupⅢ:positive in the parasitologic and serologic tests(100%,14 of 14),presented 85.7%(12 of 14)of positivity by ITS1 PCR and kDNA ***1 PCR RFLP showed that 83.3%(10 of 12)of the canine samples contained parasites with profiles similar to ***;groupⅣpresented amplifications by ITS1 PCR and kDNA ***1 PCR products were analyzed by RFLP,generating a profile similar to

关键词： Leishmania infantum Molecular diagnosis Visceral leishmaniasis PCR RFLP

The short form of the SUR1 and its functional implications in the damaged brain

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Neural Regeneration Research 2022年第3期17卷 488-496页

作者： Iván Alquisiras-Burgos Javier Franco-Pérez Moisés Rubio-Osornio Penélope Aguilera laboratorio de Patología Vascular Cerebral Instituto Nacional de Neurología y Neurocirugía“Manuel Velasco Suárez”CDMXMexico laboratorio de Formación Reticular Instituto Nacional de Neurología y Neurocirugía“Manuel Velasco Suárez”CDMXMexico laboratorio Experimental de Enfermedades Neurodegenerativas Instituto Nacional de Neurología y Neurocirugía“Manuel Velasco Suárez”CDMXMexico.

Sulfonylurea receptor(SUR) belongs to the adenosine 5′-triphosphate(ATP)-binding cassette(ABC) transporter family;however,SUR is associated with ion channels and acts as a regulatory subunit determining the opening or closing of the ***8 and Abcc9 genes code for the proteins SUR1 and SUR2,*** SUR1 transcript encodes a protein of 1582 amino acids with a mass around 140–177 k Da expressed in the pancreas,brain,heart,and other *** is well known that SUR1 assembles with Kir6.2 and TRPM4 to establish K_(ATP) channels and non-selective cation channels,***8 and 9 are alternatively spliced,and the resulting transcripts encode different isoforms of SUR1 and SUR2,which have been detected by different experimental ***,the use of binding assays to sulfonylureas and Western blotting has allowed the detection of shorter forms of SUR(-65 k Da).Identity of the SUR1 variants has not been clarified,and some authors have suggested that the shorter forms are ***,immunoprecipitation assays have shown that SUR2 short forms are part of a functional channel even coexisting with the typical forms of the receptor in the *** evidence confirms that the structure of the short forms of the SURs is fully functional and does not lose the ability to interact with the *** structural changes in short forms of SUR modify its affinity to ATP,regulation of its expression might represent an advantage in pathologies where ATP concentrations decrease and a therapeutic target to induce ***,the expression of SUR1 variants might be induced by conditions associated to the decrease of energetic substrates in the brain(*** stroke and epilepsy).In this review,we want to contribute to the knowledge of SUR1 complexity by analyzing evidence that shows the existence of short SUR1 variants and its possible implications in brain function.

关键词： brain edema epilepsy Parkinson's disease stroke sulfonylurea receptor 1 SUR1 traumatic brain injury TRPM4

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Tribological behaviour of sintered iron based self-lubricating composites

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Friction 2017年第3期5卷 285-307页

作者： Jose Daniel Biasoli DE MELLO Cristiano BINDER Gisele HAMMES Roberto BINDER Aloisio Nelmo KLEIN laboratorio de Materiais(LabMat) Universidade Federal de Santa Catarina Florianópolis 88.040-900 SC BrazilLaboratorio de Tribologia e Materiais Universidade Federal de Uberl(a)ndia Uberl(a)ndia 38.400-902 MG Brazil laboratorio de Materiais(LabMat) Universidade Federal de Santa Catarina Florianópolis 88.040-900 SC Brazil Whirlpool Embraco Unit Joinville 89.219-900 SC Brazil

This work is a review of previous works,presenting and discussing the most important results obtained by an ongoing research program towards the development of innovative,low-cost,self-lubricating composites with a low friction coefficient and high mechanical strength and wear *** emphasis is given to uniaxial die pressing of solid lubricant particles mixed with matrix powders and to metal injection moulding associated with in situ generation of solid lubricant ***,a microstructural model/processing route (powder injection moulding followed by plasma-assisted debinding and sintering) produced a homogeneous dispersion of in situ generated solid lubricant *** nodules of graphite with diameter smaller than 20 μm were formed,constituting a nanostructured stacking of graphite foils with nanometric *** Raman analysis indicated that the graphite nodules were composed of turbostratic 2D graphite having highly misaligned graphene planes separated by large interlamellae *** interplanar distance between the graphene foils and misalignment of these foils were confirmed by transmission electron microscopy and were,probably,the origin of the outstandingly low dry friction coefficient (0.04).The effect of sintering temperature,precursor content,metallic matrix composition and surface finish is also ***,the influence of a double-pressing/double-sintering (DPDS) technique on the tribological performance of self-lubricating uniaxially die-pressed hBN + graphite-Fe-Si-C-Mo composite is also ***,the tribological behaviour of die-pressed Fe-Si-C matrix composites containing 5,7.5 and 10 wt％ solid lubricants (hBN and graphite) added during the mixing step is analysed in terms of mechanical properties and wear ***,the synergy between solid lubricant particles dispersed in a metallic matrix and fluid lubricants in a cooperative mixed lubrication regime is presented.

关键词： tribological behaviour powder metallurgy iron based self-lubricating composites turbostratic graphite

Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro

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Asian Pacific Journal of Tropical Biomedicine 2019年第2期9卷 66-72页

作者： Jean Carlos Vencioneck Dutra Juda Ben-Hur de Oliveira Vanessa Silva dos Santos Paula Roberta Costalonga Pereira Jean Moises Ferreira Maria do Carmo Pimentel Batitucci laboratorio de Genetica Vegetal e Toxicologica Departamento de Ciencias BiologicasUniversidade Federal do Espirito Santo laboratorio de Biologia Molecular e Expressao Genica Departamento de Ciências BiologicasUniversidade Federal de Alagoas

Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe^(2+) ion chelating activity, and in vitro antiproliferative effects(sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe^(2+) ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.

关键词： Mandacaru Flavonoid content DPPH ABTS Fe^(2+) ion chelating activity MTT assay Sarcoma 180 cells