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deciphering the role of monosaccharides during phage infection of Staphylococcus aureus

Nano Research 2022年第10期15卷 9234-9242页

作者： Baptiste Arbez Marion Gardette Christophe Gantzer Neus Vilà Isabelle Bertrand Sofiane El-Kirat-Chatel laboratoire de chimie physique et microbiologie pour les Matériaux et l'environnement(lCPME) CNRSUniversité de Lorraine405 rue de Vandoeuvre54602 Villers-lès-NancyFrance

As phages are extensively investigated as novel therapy tools but also as transfer agents for antibiotic resistance genes,thorough understanding of phage–host interactions becomes *** for phage infection is its adhesion to the host ***,we used atomic force microscopy-based single-particle force spectroscopy with phage-decorated tips to decipher the adhesion of phage 187 on living Staphylococcus aureus *** found that addition of free N-acetyl-Dglucosamine was able to decrease phage adhesion,suggesting that this monosaccharide plays major role in phage 187 infection of ***,phage 187 adhesion on monosaccharide-coated model surfaces combined with plaque forming unit counts suggested that a direct link can be established between the propensity to bind to a saccharide and the capability of the latter to inhibit phage *** a nanoscale level,single-particle force spectroscopy was successfully used to identify a major receptor required for phage 187 infection of *** but also evidenced that this receptor was responsible for phage adhesion on *** work demonstrates that single-particle force spectroscopy is a powerful platform to screen and predict the molecular target of phages on their host surfaces.

关键词： atomic force microscopy single-particle force spectroscopy phage 187 Staphylococcus aureus phage-bacteria interactions

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Phosphorus and Nitrogen Removal by Saturated Vertical-Flow Constructed Wetlands (SVCWs) Type Micro-Pilot Using Shale from Ivory Coast as a Substrate

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Journal of Geoscience and Environment Protection 2022年第8期10卷 24-38页

作者： Dan Eude Kpannieu Norbert Kouakou Kouadio N’Dri Séraphin Konan Coulibaly Nagnonta Hippolyte Martine Mallet lacina Coulibaly Christian Ruby laboratoire de chimie physique et microbiologie pour les materiaux et l’environnement LCPME UMR 7564 CNRS Université de Lorraine Villers-lès-Nancy France UFR Ingénierie Agronomique Forestière et environnement Université de Man BP 20 Man C&#244 te d’Ivoire UFR Sciences et Technologies Université de Man BP 20 Man C&#244 te d’Ivoire laboratoire D’environnement et Biologie Aquatique Université Nangui-Abrogoua Abidjan C&#244 te d’Ivoire

This study evaluates the performance of shale from Ivory Coast used as substrate in vertical-flow constructed wetlands in removal of phosphates and nitrogen. The pilot-scale artificial wetland has been duplicated: filter planted with Panicum maximun and unplanted. They were set up outdoors, and fed with a municipal wastewater. The wetlands have been fed with three batches per week (intermittent) over a period of 3 months. During the operation period, the hydraulic residence time (HRT) 52 h was used, while wastewater temperatures varied from about ~33°C. The removal performance of the constructed wetland units was very good, since it reached on an average 98%, 89.4%, 89.4%, 84%, 80%, 84.8% and 92% for TSS, DOC, BOD5, , TKN, TP and respectively. In addition, the vegetation did not demonstrate superior performance to unplanted controls. Therefore, this study focuses on the role of shale in the phosphorus and nitrogen removal from wastewater by constructed wetland.

关键词： Constructed Wetlands Vertical Flow Shale Panicum maximun Phosphorus Removal Nitrogen

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Physico-Chemical Pollution and Trophic State of Biétry Bay (Ebriélagoon, Abidjan, Ivory Coast)

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Journal of Materials Science and Chemical Engineering 2022年第5期10卷 87-99页

作者： Dan Eude Kpannieu Kouakou Norbert Kouadio Ignace Christian M’Bra Coulibaly Nagnonta Hippolyte lacina Coulibaly S. Ouffouet laboratoire de chimie physique et microbiologie pour les Matériaux et l’environnement Université de Lorraine Villers-lès-Nancy France UFR Ingénierie Agronomique Forestière et environnement Université de Man Man C&#244te d’Ivoire laboratoire Thermodynamique et Physico-Chimique du Milieu (lTPCM) Université Nangui Abrogoua Abidjan Abidjan C&#244te d’Ivoire laboratoire d’environnement et Biologie Aquatique Université Nangui-Abrogoua Abidjan Cote d’Ivoire Centre Ivoirien Anti-Pollution (CIAPOl) Ministère de l’Environnement De la Salubrité et du Développement Durable (C&#244te d’Ivoire) Abidjan Cote d’Ivoire

The analysis of data from the National Observation Network (RNO) of the water quality of the Ivorian Anti-Pollution Center (CIAPOl) relating to the physico-chemical quality and the trophic state of the lagoon bays of Côte d’Ivoire has allowed apprehending the degree of pollution of the bays of Biétry. This bay is heavily polluted, overall, there is a high salinity at depth between 22.5‰ and 27.5‰, a slightly basic character with a pH oscillating between 7.8 and 8.1 for surface waters and between 7, 6 and 7.8 for bottom waters. This is a very marked surface oxygenation between 100.94% and 114.72%;a slightly elevated temperature oscillating between 28.6°C and 29°C for surface waters and between 26.6°C and 27.1°C for bottom waters. This is high turbidity due to suspended solids and high nitrogen and phosphorus concentrations as well. As a result, these waters evolve towards a “mesotrophic” character according to the OECD (1982) and they are “eutrophic” according to Carlson (1977). This water is therefore impure according to the WHO concerning water intended for bathing activities.

关键词： Bietry Bays Pollution Ebrié lagoon Eutrophic

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