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Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

Asian Pacific Journal of Tropical Medicine 2022年第2期15卷 71-78页

作者： Guilherme S.Machado Rosane L.Lopes Simone S.C.Oliveira Simone P.C.Freitas Jacenir R.Santos-Mallet AndréL.S.Santos denise Feder Suzete A.O.Gomes Programa de Pós-Graduação em Ciências e Biotecnologia Instituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil laboratório de Biodiversidade de Insetos e Patógenos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil laboratório de estudos Avançados de Microrganismos emergentes e Resistentes Departamento de Microbiologia GeralInstituto de Microbiologia Paulo de GóesUniversidade Federal do Rio de JaneiroRJBrazil Fundação Oswaldo Cruz Piauí TeresinaPIBrazil laboratório Interdisciplinar de Vigilância entomológica em Díptera e Hemíptera Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroRJBrazil Universidade Iguaçu Nova IguaçuRJBrazil laboratório de biologia de Insetos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil

Objective:To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies(sites of vitellogenesis)from Rhodnius(R.)prolixus infected with Trypanosoma(T.)***:Adult virgin females of *** were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of *** epimastigotes(Macias strain).Females from the control group and all the males received parasite-free *** electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected *** profile,proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected ***:According to the ultrastructural data,*** infection elicited a degeneration process in the ovarian follicular cells of *** assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group,while a significant increase in the cysteine peptidase activity was measured in both insect ***,immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected ***:*** infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of ***.

关键词： Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease Cysteine protease

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LC–MS characterization, anti-kinetoplastide and cytotoxic activities of natural products from eugenia jambolana Lam. and eugenia uniflora

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Asian Pacific Journal of Tropical Biomedicine 2017年第9期7卷 836-841页

作者： Celestina elba Sobral de Souza Ana Raquel Pereira da Silva Janaina esmeraldo Rocha Maria Celeste Vega Gomez Míriam Rolóm Cathia Coronel José Galberto Martins da Costa Manoel L.C.Netto Larissa A.Rolim Henrique Douglas Melo Coutinho laboratório de microbiologia e biologia molecular Universidade Regional do Cariri Center for the development of Scientific Research Moisés Bertoni Foundation/Díaz Gill Laboratories laboratório de Pesquisa de Produtos Naturais Universidade Regional do Cariri Centro Universitário Dr.Leao Sampaio–UNILeAO Universidade Federal do Vale do Sao Francisco–UNIVASF

Objective: To evaluate the trypanocidal, leishmanicidal and cytotoxic activity of eugenia jambolana(e. jambolana) and eugenia uniflora(e. uniflora) extracts and ***: The products were characterized by LC–MS. Antiparasitic assays were performed and cytotoxicity was evaluated in fibroblastos. In vitro assays were performed using spectrophotometric evaluation. All assays were performed in ***: The results showed that the extracts and the tannic fraction from e. jambolana inhibited 100% of the epimastigote lines. The ethanolic extract was the most efficient in all concentrations tested against the three parasite strains. In the cytotoxicity assay the flavonoid fraction showed low toxicity. All e. uniflora samples showed cytotoxicity at the highest concentration tested, but the extract showed no toxic effect on the fibroblasts at the lowest concentration. The flavonoid and tannic fractions were more efficient against Leishmania braziliensis promastigotes compared to the extract. However, the extracts and the tannic fraction were more effective against Leishmania infantum strains. The effect on epimastigote cells was observed at all concentrations tested, with all e. uniflora samples. However, the samples were more effective at the highest concentration, where there was inhibition in 100% of the Trypanosoma cruzi ***: The species e. jambolana and e. uniflora presented antiparasitic activity against all tested parasite strains, indicating that these species can serve as an alternative therapy as they were efficient in the tests performed. The e. uniflora extract and the e. jambolana flavonoid fraction presented a low cytotoxicity, opening the floor for new biological studies.

关键词： Antiparasitic effect Cytotoxicity eugenia jambolana eugenia uniflora

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Tritrichomonas foetus:New structures by high-resolution scanning helium ion microscopy

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BIOCeLL 2021年第2期45卷 259-266页

作者： MARLeNe BeNCHIMOL ABIGAIL MIRANDA-MAGALHÃeS ANTONIO PeReIRA-NeVeS WANdeRLeY de SOUZA Universidade do Grande rio Duque de Caxias25071-202Rio de Janeiro departamento de microbiologia Laboratório de Biologia Celular de PatógenosFiocruzInstituto Aggeu MagalhãesRecife52060-269Brazil Universidade Federal do rio de Janeiro Instituto de Biofísica Carlos Chagas FilhoLaboratório de Ultraestrutura Celular Hertha Meyer Instituto Nacional de Ciência e Tecnologia(INBEB)and Centro Nacional de Biologia Estrutural e Bioimagens(CENABIO)Rio de Janeiro21941-902Brazil

Helium ion scanning microscopy(HIM)is a novel high-resolution scanning microscopy technique that uses helium ions instead of electrons to form images of the highest quality and resolution,providing a sub-nanometer resolution sputter uncoated biological ***,we took advantage of HIM to explore the cell surface of Tritrichomonas foetus,a protist parasite of cattle that provokes hard infection and abortion in *** describe thin protrusions,like nanotubes described in other cells,with different sizes(27 nm to 81 nm in thickness)and various lengths(from 73 nm to 2μm),as well bulbous structures either budding from the cell surface or present in the extremities of some *** flagella also presented these thin protrusions and different protein decoration,similar to those previously described using freeze-fracture *** between two cells were also seen,and their role in infection is *** cell surface was also examined and showed several pits indicative of endocytic activity and other types of arrays of *** observations were confirmed using Scanning electron Microscopy(SeM),negative staining,and conventional thin sectioning for observation by transmission electron *** findings provide new and relevant information that may contribute to a better understanding of protozoan biology and its interaction with mammalian cells.

关键词： Nanotubes Microvesicles Surface specializations

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Synaptic pathology in multiple sclerosis:a role for Nogo-A signaling in astrocytes?

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Neural Regeneration Research 2023年第1期18卷 127-128页

作者： Sheila espírito-Santo Vinícius Gabriel Coutinho Flávia Carvalho Alcantara Gomes laboratório de biologia Celular e Tecidual Universidade Estadual do Norte Fluminense Darcy RibeiroCampos dos GoytacazesRJBrazil laboratório de Neurobiologia Celular Universidade Federal do Rio de JaneiroInstituto de Ciências BiomédicasRio de JaneiroRJBrazil

Multiple sclerosis(MS)is characterized as an inflammatory demyelinating disease that affects the central nervous system(CNS),leading to sensory,motor and cognitive ***,axonal denudation culminates in axonal lesions and *** demyelinating lesions in MS are associated with infiltration of immune cells combined with activation of the resident CNS inflammatory cells,astro-and microglia.

关键词： sclerosis impairment lesions

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Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients

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World Journal of Gastroenterology 2014年第12期20卷 3327-3334页

作者： Ana Teresa P Carvalho Barbara C esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza Disciplina de Gastroenterologia e endoscopia Digestiva Universidade do Estado do Rio de Janeiro Programa de Carcinogênese molecular Instituto Nacional de Cancer laboratório de Toxicologia e biologia molecular Universidade do Estado do Rio de Janeiro Servi?o de Gastroenterologia Departamento de Clínica MédicaUniversidade Federal do Rio de Janeiro

AIM: To analyze the prevalence of thiopurine-methyltransferase (TPMT) genotypes and their association with drug toxicity in inflammatory bowel disease (IBD) patients from southeastern Brazil.

关键词： Inflammatory bowel disease Thiopurine-methyl-transferase Azathioprine Drug toxicity Pancreatitis

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Does cypermethrin affect enzyme activity, respiration rate and walking behavior of the maize weevil （Sitophilus zeamais）？

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Insect Science 2013年第3期20卷 358-366页

作者： Ronnie Von Santos Veloso eliseu Jose G. Pereira Raul Narciso C. Guedes Maria Goreti A. Oliveira departamento de entomologia departamento de Bioquimica e biologia molecular Universidade Federal de Vicosa Brazil

Insecticides cause a range of sub-lethal effects on targeted insects, which are frequently detrimental to them. However, targeted insects are able to cope with insecti- cides within sub-lethal ranges, which vary with their susceptibility. Here we assessed the response of three strains of the maize weevil Sitophilus zeamais Motschulsky （Coleoptera.＂ Curculionidae） to sub-lethal exposure to the pyrethoid insecticide cypermethrin. We ex- pected enzyme induction associated with cypermethrin resistance since it would aid the resistant insects in surviving such exposure. Lower respiration rate and lower activity were also expected in insecticide-resistant insects since these traits are also likely to favor survivorship under insecticide exposure. Curiously though, cypermethrin did not affect ac- tivity of digestive and energy metabolism enzymes, and even reduced the activity of some enzymes （particularly for cellulase and cysteine-proteinase activity in this case）. There was strain variation in response, which may be （partially） related to insecticide resistance in some strains. Sub-lethal exposure to cypermethrin depressed proteolytic and mainly cellulolytic activity in the exposed insects, which is likely to impair their fitness. However, such exposure did not affect respiration rate and walking behavior of the insects （except for the susceptible strain where walking activity was reduced）. Walking activity varies with strain and may minimize insecticide exposure, which should be a concern, particularly if associated with （physiological） insecticide resistance.

关键词： insecticides insecticide resistance pyrethroids stored grain insect sub-lethal effects

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Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 363-370页

作者： Daniel Barroso de Alencar Fátima Cristiane Teles de Carvalho Rosa Helena Rebouas Daniel Rodrigues dos Santos Kelma Maria dos Santos Pires-Cavalcante Rebeca Larangeira de Lima Bárbara Mendes Baracho Rayssa Mendes Bezerra Francisco Arnaldo Viana Regine Helena Silva dos Fernandes Vieira Alexandre Holanda Sampaio Oscarina Viana de Sousa Silvana Saker-Sampaio laboratrio de Produtos Naturais Marinhos.departamento de engenharia de Pesca Universidade Federal do Ceará.Campus do Pici.Ar.Mister Hull.s/nCaixa Postal 6043 laboratrio de microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará.Av.da Abolicao 3207 laboratrio de microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará laboratrio de Produtos Naturais Marinhos.departamento de engenharia de Pesca Universidade Federal do Ceará laboratrio de Cromatografia Departamento de QuimicaUniversidade do Estado do Rio Grande do NorteCampus Universitário Central

Objective:To evaluate the antioxidant,antibacterial and bacterial cell agglutination activities of the hexane(Hex) and 70%ethanol(70%etOH) extracts of two species of red seaweeds Pterocladiella capillacea(***) and Osmundaria ***:In vitro antioxidant activity was determined by DPPH radical scavenging assay,ferric-reducing antioxidant power assay,ferrous ion chelating assay,β-carotene bleaching assay and total phenolic content *** activity was tested using the method of disc diffusion on Mueller-Hinton *** ability of algal extracts to agglutinate bacterial cells was also ***:The 70%etOH extract of the two algae showed the highest values of total phenolic content compared to the Hex *** results of DPPH for both extracts(Hex,70%etOH) of Osmundaria obtusiloba(43.46%and 99.47%) were higher than those of ***(33.04%and 40.81%) at a concentration of 1 000 μg/*** for the ferrous ion chelating,there was an opposite behavior,extracts of *** had a higher *** extracts showed a low ferric-reducing antioxidant power,with optical density ranging from0.054 to *** activities of all extracts evaluated for β-carotene bleaching were above 40%.There was no antibacterial activity against bacterial strains ***,the extracts of both species were able to agglutinate bacterial Gram positive cells of Staphylococcus aureus and Gram negative cells of escherichia coli,multidrug-resistant Salmonella and Vibrio *** This is the first report of the interaction between these algal extracts,rich in natural compounds with antioxidant potential,and Gram positive and Gram negative bacterial cells.

关键词： Bioactive compounds Antioxidant Bacterial agglutination Rhodophyta

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Mayaro fever:A brief review on the immune profile

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Asian Pacific Journal of Tropical Biomedicine 2020年第3期10卷 95-100页

作者： Jean Moisés Ferreira Danielly Santos Campos-Ferreira elaine Virgínia Martins de Sousa Figueiredo Jose Luizde Lima Filho laboratório de Imunopatologia Keizo Asami-LIKA Centro de BiociênciasUniversidade Federal de Pernambuco(UFPE)50670-901RecifePernambucoBrazil laboratório de biologia molecular e expressao Genica-LABMeG Departamento de Ciências BiológicasUniversidade Federal de Alagoas(UFAL)Campus Arapiraca57300-970ArapiracaAlagoasBrazil

Mayaro virus is an emergent alphavirus that infects humans,leading to Mayaro *** fifty percent of infected patients develop arthritis symptoms in the recovery phase,a phase that can last up to a *** literature about Mayaro virus infection and its immune response is scarce,which may hamper the development of treatment *** summarize changes in cytokines and chemokines in the acute and recovery phase in Mayaro virus infected patients,and relate this molecular characterization with the immune *** and IL-12/p70 show pronounced changes in patients in the acute phase,suggesting the development of cellular immunity and Th1 ***-6,IL-7,CXCL8/IL-8,IL-13,IL-17,and IFN-γare elevated in patients with arthritis symptoms in the long-term recovery phase,which may be related to the continuous inflammatory process,a possible Th2 inhibiting and promoting Th17 *** few studies discuss the issue,with a small number of patients and different backgrounds,inflammatory and immune response and manifestations seem to be closely *** information may help to develop the appropriate treatment strategies in Mayaro virus ***,we analyzed and summarized data available in literature.

关键词： emergent virus Mayaro virus Cytokines Chemokine molecular characterization

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Tuning mesenchymal stem cell secretome therapeutic potential through mechanotransduction

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BIOCeLL 2022年第6期46卷 1375-1381页

作者： GIORDANO WOSGRAU CALLONI MARCO AUGUSTO STIMAMIGLIO laboratório de Plasticidade e Diferenciação de Células da Crista Neural Departamento de Biologia CelularEmbriologia e GenéticaCentro de Ciências BiológicasUniversidade Federal de Santa CatarinaCampus Universitário–TrindadeCEPFlorianópolis88040-900Brazil laboratório de biologia Básica de Células-Tronco Instituto Carlos ChagasFiocruz/PRCEPCuritiba81350-010Brazil

Mesenchymal stem cells(MSCs)and their byproducts have been widely validated as potential therapeutic products for regenerative *** therapeutic effects result mainly from the paracrine activity of MSCs,which consists of the secretion of bioactive molecules,whether dispersed in medium conditioned by cell culture or encapsulated in extracellular *** composition of the MSC secretome,which represents the set of these secreted cellular products,is crucial for the performance of the desired therapeutic *** cell culture strategies have been employed to adjust the secretome composition of MSCs to obtain the best therapeutic responses for different clinical ***,the manipulation of culture conditions has focused mainly on the use of different biochemical elements for the preconditioning of MSCs and less on the physical conditions of the cell culture ***,we offer our point of view regarding the importance of the physical properties of cell culture substrates and their mechanotransduction responses in preconditioning the MSCs *** highlight the relevance of studying mechanotransduction events associating cell morphology and the modulation of gene expression to customize and expand the use of MSCs secretomes.

关键词： Mesenchymal stem cells Mechanotransduction Scaffolds Secretome Bioengineering

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Autophagy, apoptosis and organelle features during cell exposure to cadmiumč

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BIOCeLL 2013年第2期37卷 45-54页

作者： CRISTIANe DOS SANTOS VeRGILIO eDÉSIO JOSÉTeNÓrio de MeLO Universidade estadual do Norte Fluminense Centro de Biociências e BiotecnologiaLaboratório de Biologia Celular e TecidualCampos dos GoytacazesRJBrasil28013-602.

Cadmium(Cd)induces several effects in different tissues,but our knowledge of the toxic effects on organelles is insuffi *** observe the progression of Cd effects on organelle structure and function,HuH-7 cells(human hepatic carcinoma cell line)were exposed to CdCl2 in increasing concentrations(1μM–20μM)and exposure times(2 h–24 h).During Cd treatment,the cells exhibited a progressive decrease in viability that was both time-and *** treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell *** with 10μM for 12 h led to irreversible *** these drastic and irreparable damages,treated cells(5μM for 12 h)presented a progressive loss of mitochondrial function and cytoplasm acidifi cation as well as dysfunction and disorganization of microfi laments and endoplasmic *** damages led to the induction of apoptotic events and an increase in autophagic bodies in the *** results revealed that Cd affects multiple intra-cellular targets that induce alterations in the mitochondria,cytoskeleton,endoplasmic reticulum and acidic compartments,ultimately culminating in cell death via apoptotic and autophagic pathways.

关键词： Cd cell death hepatocyte HuH-7 cells mitochondria

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