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Intracellular life of protozoan Toxoplasma gondii:Parasitophorous vacuole establishment and survival strategies

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BIOCeLL 2023年第4期47卷 929-950页

作者： JULIANA A.PORTeS ROSSIANe C.VOMMARO LUCIO AYReS CALDAS eRICA S.MARTINS-DUARTe laboratório de Ultraestrutura Celular Hertha Meyer Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil Núcleo Multidisciplinar de Pesquisa UFRJ–Xerém em biologia Campus Duque de CaxiasUniversidade Federal do Rio de JaneiroDuque de CaxiasBrazil laboratório de Quimioterapia de Protozoários egler Chiari Departamento de ParasitologiaUniversidade Federal de Minas GeraisBelo HorizonteBrazil

Toxoplasma gondii is a protozoan of worldwide distribution and the agent of *** is estimated that 30%–50%of the world population could be infected with this *** the infection in immunocompetent individuals is mostly asymptomatic,the disease in immunosuppressed and pregnant is a risk *** a member of the phylum Apicomplexa,*** has an obligatory intracellular lifestyle;therefore,invading a host cell and establishing it inside a parasitophorous vacuole(PV)are mandatories for the survival of this *** construction of a perfect intracellular niche for *** requires the secretion of an arsenal of proteins from unique secretory *** proteins will remodel the vacuolar environment and the host cell organization and functions,allowing the parasite to access essential nutrients and stay“invisible”inside a host *** the present review,we will discuss the main steps involved in the PV formation and its differentiation to tissue cyst,focusing mainly on the strategies employed in the acquisition of nutrients and proteins involved in host cell modification.

关键词： dense granule proteins Rhoptry proteins Nutrient acquisition Intravacuolar network Immune evasion Tissue cyst

Changes in cellular proliferation and plasma products are associated with liver failure

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World Journal of Hepatology 2016年第32期8卷 1370-1383页

作者： Juliana Gil Melgaco Frederico Marianetti Soriani Pedro Henrique Ferreira Sucupira Leonardo Assaf Pinheiro Yasmine Rangel Vieira Jaqueline Mendes de Oliveira Lia Laura Lewis-Ximenez Cristina Carvalho Vianna Araújo Lúcio Filgueiras Pacheco-Moreira Gustavo Batista Menezes Oswaldo Goncalves Cruz Claudia Lamarca Vitral Marcelo Alves Pinto departamento de Microbiologia e parasitologia Universidade Federal Fluminense Instituto Oswaldo Cruz/Fiocruz Laboratório de Desenvolvimento Tecnológico em Virologia departamento de biologia Geral Instituto de Ciencias BiologicasUniversidade Federal de Minas Gerais Instituto Oswaldo Cruz/Fiocruz Ambulatório de Hepatites Virais Hospital Federal de Bonsucesso laboratório de Imunofarmacologia Departamento de Bioquímica e ImunologiaInstituto de Ciencias BiológicasUniversidade Federal de Minas Gerais Fundacao Oswaldo Cruz Programa de Computacao Científica

AIM To study the differences in immune response and cytokine profile between acute liver failure and selflimited acute *** Forty-six patients with self-limited acute hepatitis(AH), sixteen patients with acute liver failure(ALF), and twenty-two healthy subjects were involved in this study. The inflammatory and anti-inflammatory products in plasma samples were quantified using commercial enzyme-linked immunoassays and quantitative real-time PCR. The cellular immune responses were measured by proliferation assay using flow cytometry. The groups were divided into viral- and non-viral-induced selflimited AH and ALF. Thus, we worked with five groups: Hepatitis A virus(HAV)-induced self-limited acute hepatitis(HAV-AH), HAV-induced ALF(HAV-ALF), nonviral-induced self-limited acute hepatitis(non-viral AH), non-viral-induced acute liver failure(non-viral ALF), and healthy subjects(HC). Comparisons among HAV and non-viral-induced AH and ALF were *** The levels of mitochondrial DNA(mt DNA) and the cytokines investigated [interleukin(IL)-6, IL-8, IL-10, interferon gamma, and tumor necrosis factor] were significantly increased in ALF patients, independently of etiology(P ere the best markers associated with ALF [mt DNA: OR = 320.5(95%CI: 14.42-7123.33), P death [mt DNA: OR = 12.1(95%CI: 2.57-57.07), P = 0.002; and IL-10: OR = 8.01(95%CI: 1.26-50.97), P = 0.027]. In the cellular proliferation assay, NK^(bright), NKT and regulatory T cells(TReg) predominated in virusspecific stimulation in HAV-induced ALF patients with an anergic behavior in the cellular response to mitotic stimulation. Therefore, in non-viral-induced ALF, anergic behavior of activated T cells was not observed after mitotic stimulation, as expected and as described by the literature. CONCLUSION mt DNA and IL-10 may be predictors of ALF and death. TReg cells are involved in immunologi

关键词： Acute liver failure Cytokines Mitochondrial DNA Cellular immune response Hepatitis A virus

A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

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Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 62-70页

作者： Natalia Souza de Godoy Manoel Sebastiao da Costa Lima-Junior Jose Angelo Lauletta Lindoso Vera Lucia Pereira-Chioccola Thelma Suely Okay Lucia Maria Almeida Braz laboratorio de parasitologia Médica Departamento de Moléstias Infecciosas e Parasitárias do Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil laboratorio de Imunopatologia e biologia Molecular-Instituto Aggeu Magalhaes/IAM-FIOCRUZ RecifeBrazil laboratorio de Soroepidemiologia e Imunobiologia-FMUSP Sao PauloBrazil laboratorio de biologia Molecular de Parasitas e Fungos-Instituto Adolfo Lutz Sao PauloBrazil laboratorio de parasitologia-Instituto de Medicina Tropical de Sao Paulo-USP Sao PauloBrazil

Objective:To determine an algorithm for molecular diagnosis of visceral leishmaniasis(VL)by kinetoplast DNA(kDNA)(RV1/RV2)and internal transcriber spacer(ITS1)(LITSR/L5.8 S)polymerase chain reaction(PCR),complemented by ITS 1 PCR restriction fragment length polymorphism(RFLP),using peripheral blood or bone marrow aspirate from patients with suspected ***:Biological samples were submitted to the gold standard for the diagnosis of VL and molecular diagnosis represented by ITS 1 PCR,kDNA PCR,and ITS 1 PCR *** samples were obtained from seven groups:groupⅠ,82 samples from patients with confirmed VL;groupⅡ,16 samples from patients under treatment for VL;groupⅢ,14 samples from dogs with canine visceral leishmaniasis(CVL);groupⅣ,a pool of six experimentally infected sandflies(Lutzomya longipalpis);group V,18 samples from patients with confirmed tegumentary leishmaniasis(TL)and groupsⅥandⅦwere from control groups without ***:The following gold standard and molecular examination results were obtained for each of the seven groups:groupⅠ:parasitologic and immunochromatographic tests showed a sensitivity of 76.3%(61 of 80)and 68.8%(55 of 80),respectively,and a sensitivity of 97.6%(80 of 82)and 92.7%(76 of 82)by ITS1 and kDNA PCR,*** ITS1 PCR RFLP(HaeⅢ)analysis of the 80 positive samples,52.5%(42 of 80)generated three fragments of 180,70,and 50 bp,corresponding to the pattern of Leishmania infantum infantum;groupⅡ:negative for the parasitologic methods and positive for IrK39(100%,16 of 16),presented 12.5%(2 of 16)of positivity by ITS 1 PCR and 25.0%(4 of 16)by kDNA PCR;groupⅢ:positive in the parasitologic and serologic tests(100%,14 of 14),presented 85.7%(12 of 14)of positivity by ITS1 PCR and kDNA ***1 PCR RFLP showed that 83.3%(10 of 12)of the canine samples contained parasites with profiles similar to ***;groupⅣpresented amplifications by ITS1 PCR and kDNA ***1 PCR products were analyzed by RFLP,generating a profile similar to

关键词： Leishmania infantum Molecular diagnosis Visceral leishmaniasis PCR RFLP

What a few hairs can tell us about the resource use of giant armadillos

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Integrative Zoology 2023年第1期18卷 129-142页

作者： Marcelo MAGIOLI Nina ATTIAS Gabriel MASSOCATO Danilo KLUYBeR Marcelo Zacharias MOReIRA Katia Maria Paschoaletto Micchi de Barros FeRRAZ Adriano Garcia CHIAReLLO Arnaud L.J.deSBIeZ Instituto Pró-Carnívoros AtibaiaSão PauloBrazil Centro Nacional de Pesquisa e Conservação de mamíferos Carnívoros(CeNAP) Instituto Chico Mendes de Conservação da Biodiversidade(ICMBio)AtibaiaSão PauloBrazil Instituto de Conservação de Animais silvestres(ICAS) Campo GrandeMato Grosso do SulBrazil Instituto de Pesquisas ecológicas Organização Não GovernamentalNazaréPaulistaSão PauloBrazil Houston Zoo HoustonTexasUSA Naples Zoo at Caribbean Gardens NaplesFloridaUSA laboratório de ecologia Isotópica Centro de Energia Nuclear na AgriculturaUniversidade de São PauloPiracicabaSão PauloBrazil laboratório de ecologia Manejo e Conservação de Fauna Silvestre(LEMaC)Departamento de Ciências FlorestaisEscola Superior de Agricultura“Luiz de Queiroz”(ESALQ)Universidade de São Paulo(USP)PiracicabaSão PauloBrazil departamento de biologia Faculdade de FilosofiaCiências e LetrasUniversidade de São Paulo(USP)Ribeirão PretoSão PauloBrazil RZSS-Royal Zoological Society of Scotland(RZSS) MurrayfieldEdinburghUK

Understanding of resource requirements of vulnerable species is key for conservation ***,we used sta-ble carbon(δ13C)and nitrogen isotopes(δ15N)of hair from giant armadillos(Priodontes maximus)to understand individual resource *** collected hair from 19 giant armadillos analyzing 34 samples from the Brazilian Pan-tanal and compared the resource use between sexes and age *** also assessed the relationship of isotopic values with individuals’body mass and the habitat type *** observed a wide variation in resource use by giant armadillos,showing that individuals consume prey that feeds on distinct resources and occupies different habitat types,indicating that there is individual variation in foraging *** giant armadillos presented a mixed diet(C3/C4 resources,forests/open areas),but a quarter of individuals had strictly C4 diets(open areas).Males are more prone to forage on C4 resources,presenting an isotopic niche 2.6-times larger than that of *** presented diets more associated with habitats with high arboreal cover(C3 resources,forests),while adults foraged more in open areas(C4 resources).This result is mirrored by the positive relationship betweenδ13C values and body mass,suggesting that larger giant armadillos tend to feed more in open *** observed thatδ13C values decreased as individuals increased the use of habitats with high arboreal *** stress the importance of con-serving the natural mosaic in the Pantanal landscape for the species persistence,given that giant armadillos require all habitat types to fulﬁll their dietary,spatial,and developmental needs.

关键词： diet ontogenetic changes resource partitioning stable isotopes telemetry

Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area

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Infectious Diseases of Poverty 2019年第3期8卷 99-100页

作者： Sergei Rodrigo Magalhaes de Sousa Isabelle Helena Lima Dias Alvaro Luan Santana Fonseca Bianca Rodrigues Contente Joyce Favacho Cardoso Nogueira Tatyellen Natasha da Costa Oliveira Stefan Michael Geiger Martin Johannes enk Programa de Pos-Graduacao Strictu Sensu em biologia Parasitaria na Amazonia da Universidade do estado do Para Tv.Perebebui2623-MarcoBelemPA 66087Brazil laboratorio de Parasitoses Intestinais Esquistossomose e Malacologia(LPIEM)Secgao de ParasitologiaInstituto Evandro Chagas/SVS/MSAnanindeuaParaBrazil

Background:The Kato-Katz technique is recommended worldwide for the diagnosis of intestinal schistosomiasis,detecting parasite eggs in feces of infected ***,new tests have been developed in order to facilitate diagnosis,*** detection of specific antigens secreted by schistosomes,such as the circulating cathodic antigen(CCA).The aim of this study was to evaluate the performance of the point-of-care circulating cathodic antigen test(POC-CCA)compared to the Kato-Katz technique in a low prevalence area in the Amazon Region,located in the municipality of Primavera,State of Para,***:Positivity rates of the POC-CCA test and the Kato-Katz technique were *** sensitivity,specificity,accuracy and kappa coefficient were determined by comparing both *** reference standard was established using 16 Kato-Katz slides,12 of the first fecal sample,two of the second and two of the third *** study also included the concordance between POC-CCA results and different numbers and combinations of Kato-Katz ***:The prevalence of schistosomiasis according to the reference standard or POC-CCA test reached a rate of 9.4%or 23.9%,respectively,among a total of 372 *** positivity rates by the Kato-Katz technique increased from 2.4 to 9.4%,according to the increase in the number of slides examined and fecal samples colleaed.A sensitivity of 55.6%,specificity 76.9%,accuracy 76%and k coefficient of 0.06 was observed by comparing one slide of the first sample and *** 6 slides from three different samples,two slides of each,with POC-CCA resulted in a sensitivity of 583%,specificity 78.4%,accuracy 77%and k coefficient of ***,the comparison of 16 slides from three different samples with POC-CCA revealed a sensitivity of 65.7%,specificity 80.4%,accuracy 79%,and k coefficient of ***:The immunochromatographic test has the potential to be an important tool to combat schistosomiasis because of its practi

关键词： Schistosoma mansoni Kato-Katz POC-CCA Accuracy Diagnosis Low prevalence area

Diversity and host assemblage of avian haemosporidians in different terrestrial ecoregions of Peru

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Current Zoology 2022年第1期68卷 27-40页

作者： Luz GARCIA-LONGORIA Jaime MURIeL Sergio MAGALLANeS Zaira Hellen VILLA-GALARCe Leonila RICOPA Wilson Giancarlo INGA-DIAZ esteban FONG Daniel VeCCO Cesar GUeRRA-SALDANA Teresa SALAS-ReNGIFO Wendy FLOReS-SAAVeDRA Kathya eSPINOZA Carlos MeNDOZA Blanca SALDANA Manuel GONZALeZ-BLAZQUeZ Henry GONZALeS-PINeDO Charlene LUJAN-VeGA Carlos Alberto del AGUILA Yessica VILCA-HeRReRA Carlos Alberto PINeDA Carmen ReATeGUI Jorge Manuel CARdeNAS-CALLIRGOS JoseAlberto IANNACONe Jorge Luis MeNDOZA Ravinder NMSeHGAL Alfonso MARZAL departamento de Anatomia Biologia Celular y ZoologiaUniversidad de ExtremaduraBadajoz E-506071Spain Molecular ecology and evolution Lab Department of BiologyLund UniversitySolvegatan 37SE-22362 LundSweden Instituto Pirenaico de ecologıa-IPe(CSIC) Avda.Nuestra Senora de la Victoria 16Jaca 22700Spain DIReSA Direccion Regional de SaludLoreto 16001Peru departamento Academico de Microbiologia y parasitologia.Facultad de Ciencias Biologicas Universidad Nacional de la Amazonia PeruanaIquitos 16001Peru Facultad de Ciencias Agropecuarias Universidad Alas PeruanasLima 15823Peru everGreen Institute-San Rafael Distrito de IndianaLoreto 16200Peru Observatorio de Aves Loreto(LBO) Distrito de San JuanLoreto 16008Peru Centro Urku de estudios Amazonicos Tarapoto 22200Peru Servicio Nacional de Areas Naturales Protegidas por el estado 15036Peru Sanidad Animal-Facultad de Zootecnia Universidad Nacional Agraria La MolinaLima 15012Peru laboratorio de Microbiologia Facultad de Medicina Veterinaria y ZootecniaUniversidad CientUfica del SurLima 15067Peru laboratorio de Analisis Clinico Moraleslab SAC MoralesSan Martin 22201Peru Asociacion ecosistemas Andinos(eCOAN) Cuzco 08002Peru Pharmacology and Toxicology Graduate Group University of CaliforniaDavisDA 95616USA Global Health Initiative Wabash CollegeCrawfordsvilleIN 47933USA Facultad de Ciencias Biologicas Universidad Nacional Mayor de San MarcosLima 15081Peru Facultad de Medicina Veterinaria Universidad Nacional Hermilio ValdizanHuanuco10160Peru Asociacion Peruana de Helmintologia e Invertebrados Afines Lima 15054Peru laboratorio de ecologia y Biodiversidad Animal Universidad Nacional Federico VillarrealEl AgustinoLima 15007Peru laboratorio de Invertebrados Universidad Ricardo Palma-Santiago de SurcoLima 15537Peru department of Biology San Francisco State UniversitySan FranciscoCA 94132USA

Characterizing the diversity and structure of host–parasite communities is crucial to understandingtheir eco-evolutionary *** and related haemosporidian parasites are responsible forfitness loss and mortality in bird species ***,despite exhibiting the greatest ornithologicalbiodiversity,avian haemosporidians from Neotropical regions are quite ***,we analyze the genetic diversity of bird haemosporidian parasites(Plasmodium andHaemoproteus)in 1,336 individuals belonging to 206 bird species to explore for differences in diversityof parasite lineages and bird species across 5 well-differentiated Peruvian *** 70 different haemosporidian lineages infecting 74 bird *** showed that 25 out ofthe 70 haplotypes had not been previously ***,we also identified 81 new host–parasite interactions representing new host records for these haemosporidian *** outcomesrevealed that the effective diversity(as well as the richness,abundance,and Shannon–Weaver index)for both birds and parasite lineages was higher in Amazon basin ***,we also showed that ecoregions with greater diversity of bird species also had highparasite richness,hence suggesting that host community is crucial in explaining parasite *** parasites were found in ecoregions with lower bird diversity,implying that the abundanceand richness of hosts may shape the exploitation strategy followed by haemosporidian *** outcomes reveal that Neotropical region is a major reservoir of unidentified *** studies analyzing host distribution and specificity of these parasites inthe tropics will provide important knowledge about phylogenetic relationships,phylogeography,and patterns of evolution and distribution of haemosporidian parasites.

关键词： Amazonia avian malaria generalist parasite habitat specificity Haemoproteus Plasmodium specialist parasite

evaluation of the Anti-Trypanosoma cruzi effects of the Antipsychotic Drug Levomepromazine

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International Journal of Clinical Medicine 2012年第5期3卷 344-351页

作者： Inga eva Stik Lange elisabeth Mieko Furusho Pral Anahi Magdaleno Ariel Mariano Silber departamento de parasitologia Instituto de CienciasUniversidade de Sao PauloSao PauloBrazil

Chagas disease, caused by the protozoan Trypanosoma cruzi, is a relevant parasitic disease in the Americas. Current chemotherapy relies on Nifurtimox and Benznidazole, which present serious drawbacks, including high toxicity, low efficiency and the emergence of resistant strains. In the present work, the perspectives of levomepromazine, a tri-cyclic compound belonging to the family of phenotiazines with well-known properties as antipsychotics were evaluated as a potential anti-T. cruzi drug. We show that this drug is able to inhibit the proliferation of epimastigotes (IC50 = 0.41 ± 0.01 mM) and to interfere with the infection of the host cells (IC50 = 0.34 ± 0.01 mM). Interestingly, the treatment with levomepromazine affected the ability of metabolites such as glucose, proline and glutamate to fuel the recovery of epi-mastigotes after being submitted to metabolic stress. These findings prompt levomepromazine as a promising leader drug to obtain new trypanocidal activities.

关键词： Chagas Disease Phenothiazines Trypanothione Reductase Amino Acid Metabolism Chemotherapy

MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults

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World Journal of Gastroenterology 2013年第20期19卷 3043-3051页

作者： Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro Unidade Integrada de Farmacologia e Gastroenterologia Universidade So FranciscoBragan a Paulista 12916-900SPBrazil Programa de Pós Gradua o em Genética e biologia Molecular State University of CampinasCampinas 13083-970SPBrazil departamento de Clínica Médica UNESPBotucatu 18618-970SPBrazil departamento de Genética Universidade Federal do ParáBelém 68400-000PABrazil departamento de Microbiologia Imunologia e ParasitologiaUniversidade Federal de So PauloSo Paulo 04021-001SPBrazil

AIM:To evaluate the association between Helicobacter pylori(***) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).MeTHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or ***-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver *** analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's ***:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of *** infection *** MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with *** infection(P egion was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from ***-infected chronic gastritis patients was statistically significant(P ethylation frequencies among ***-infected and non-infected chronic gastritis adult patients were 13% and 7%,*** observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from ***-infected adult chronic gastritis patients(P espectively).The frequency of promoter methylation for both genes was higher in gastric cancer sam

关键词： Helicobacter pylori Microsatellite instability Promoter methylation MLH1 MGMT Gastric cancer

Purinergic signaling and human immunodeficiency virus/acquired immune deficiency syndrome: From viral entry to therapy

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World Journal of Virology 2015年第3期4卷 285-294页

作者： Daniela F Passos Maria Rosa C Schetinger Daniela BR Leal departamento de Microbiologia e parasitologia Centro de Ciências da SaúdeUniversidade Federal de Santa Maria departamento de Bioquímica e biologia Molecular Centro de Ciências Naturais e ExatasUniversidade Federal de Santa Maria

Human immunodeficiency virus(HIV) infection is a serious condition associated to severe immune dysfunction and immunodeficiency. Mechanisms involved in HIV-associated immune activation, inflammation and loss of CD4+ T cells have been extensively studied, including those concerning purinergic signaling pathways. Purinergic signaling components are involved in viral entry and replication and disease progression. Research involving the participation of purinergic signaling in HIV infection has been not only important to elucidate disease mechanisms but also to introduce new approaches to therapy. The involvement of purinergic signaling in the pathogenesis of HIV infection and its implications in the control of the HIV infection are reviewed in this paper.

关键词： Human immunodeficiency virus Purinergic signaling Immune activation Inflammation