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Relationship between histone acetylation/deacetylation and gene transcription

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Chinese Science Bulletin 1998年第13期43卷 1057-1063页

作者： Zhen Lu Yongchao Wang Beijing Normal Univ Key Lab Cell Proliferat & Regulat Biol Beijing 100875 Peoples R China

In eukaryotes, nucleosome is the basic unit of chromatin. Nucleosome is composed of an octamer of histone proteins (two molecules each of histones H 2A , H 2B , H 3 and H 4) and DNA strand wound around the octamer. Some data show that core histone octamer can affect gene transcription both \%in vitro\% and \%in vivo.\% Recent results indicate that histone acetylation/deacetylation is a key step to regulate activity of genes. This article summarizes some coactivators, such as GCN5p, P300/CBP and TAF Ⅱ 250, which are recently found to have histone acetyltransferase activity. The relationship between these coactivators and gene activation is also described. Besides, this article concerns some corepressors which have histone deacetylase activity, such as Rpd3p, HDAC2. These corepressors combine with other protein complex and then repress transcription. Finally, some problems to be solved and the future direction in this active field are discussed.

关键词： Histone acetylation/deacetylation transcriptional regulation.

Imide-pillared covalent organic framework protective films as stable zinc ion-conducting interphases for dendrite-free Zn metal anodes

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Journal of Energy Chemistry 2024年第10期97卷 470-477页

作者： Xiaoman Ye Xuemei Xiao Zhijing Wu Xin Wu Lin Gu Sheng Liu School of Chemical Engineering and Technology Sun Yat-sen University The Key lab.of Low-Carbon Chemistry & Energy Conservation of Guangdong Province Sun Yat-sen University

The notorious growth of zinc dendrite and the water-induced corrosion of zinc metal anodes（ZMAs）restrict the practical development of aqueous zinc ion batteries（AZIBs）.In this work,a zinc metallized,imide-pillared covalent organic framework（ZPC） protective film has been engineered as a stable Zn2+ion-conducting interphase to modulate interfacial kinetics and suppress side reactions for *** to bare Zn,ZPC@Zn exhibits a higher Zn2+ionic conductivity,a larger Zn2+transference number,a lower electronic conductivity,a smaller desolvation activation energy and correspondingly a significant suppression of corrosion,hydrogen evolution and Zn ***,the ZPC@Zn||ZPC@Zn symmetric cell obtains a cycling lifespan over 3000 h under 5 mA cm-2for 1 mA h *** ZPC@Zn||NH4V4O10coin-type full battery delivers a specific capacity of 195.8 mA h g-1with a retention rate of78.5% at 2 A g-1after 1100 cycles,and the ZPC@Zn||NH4V4O10pouch full cell shows a retention of70.1% in reversible capacity at 3 A g-1after 1100 *** present incorporation of imide-linked covalent organic frameworks in the surface modification of ZMAs will offer fresh perspectives in the search for ideal protective films for the practicality of AZIBs.

关键词： Aqueous zinc ion batteries Covalent organic frameworks Imide linkage Surface modification Zinc metal anodes

Site-specific uv crosslinking of minihelix DNA and TrpRS from Bacillus subtilis

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Chinese Science Bulletin 2001年第16期46卷 1380-1383页

作者： Xu, F Jiang, G Zhang, Y Zhu, LF Jin, YX Wang, DB Chinese Acad Sci Shanghai Inst Biol Sci Inst Biochem & Cell Biol State Key Lab Mol Biol Shanghai 200031 Peoples R China

In order to investigate the recognition mechanism and the relationship between structure and function of minihelix DNA with Tryptophanyl-tRNA Synthetase (TrpRS), TrpRS from Bacillus Subtilis was purified. Four minihelix DNAs were chemically synthesized and the photoreactive reagent s4T was incorporated into three of them at the positions of G73, T72 and T55 corresponding to tRNATrp. The apparatus for uv crosslinking was devised and the parameters for uv crosslinking were optimized. The results indicated that the G73 and T72 base of minihelix DNA interacted with TrpRS directly. The uv crosslinking reaction was improved by the dose of uv irradiation and the concentration of both TrpRS and minihelix DNA.

关键词： uv crosslinking minihelix DNA TrpRS discriminator.

Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase

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Chinese Science Bulletin 2001年第8期46卷 683-685页

作者： Gaoxiang Ge Jing Wu Qishui Lin Chinese Acad Sci Shanghai Inst Biol Sci Inst Biochem & Cell Biol State Key Lab Mol Biol Shanghai 200031 Peoples R China

A non-radioisotopic method was developed for the assay of epidermal growth factor receptor (EGFR). A peptide with twenty amino acid residues around Tyr 1173, the major phosphorylation site of EGFR, was cloned as a GST fusion protein and used as substrate. Anti-phosphoty-rosine monoclonal antibody PY99 was used for the determination of the extent of phosphorylation. Both the specificity and the sensitivity were substantially higher than that of the existing method. Km value of the fusion protein is much lower (10 μmol/L) than that of the synthetic peptide (110 μmol/L). The method can be applied to the measurement of the tyrosine kinase activity of c-erb B2 (Neu/HER2).

关键词： EGF receptor tyrosine kinase nonradioisotopic GST in vitro.

P15——a new tumor suppressor gene

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Chinese Science Bulletin 1999年第13期44卷 1157-1163页

作者： Yingkai Tong Huitu Liu Beijing Normal Univ State Key Lab Cell Proliferat & Regulat Biol Beijing 100875 Peoples R China

In addition to the tumor suppressor genes such as Rb and p53, it has been found that some molecules of the same class named CKI (cyclin-dependent kinase inhibitor) also play an important role in the inhibition of tumorigenesis and the tumor progression. In the KIP and INK4 families of CKIs, p15 shares extensive homology with p16. Findings in many tumors and their cell lines show that the inactivation of p15 (deletion, mutation, rearrangement, etc.) is very frequent, and inactive p15 is involved in the progress of some tumors. These studies provide evidence that the p15 is a new tumor suppressor gene. Furthermore, the research on the molecular mechanism of p15 in regulation of cell proliferation shows that p15 can inhibit the growth of some kinds of tumor cells, and p15 is the mediator of TGF-β-induced cell arrest. Investigations on p15 in cell differentiation suggest that increased p15 is related to the change of malignant phenotype. These results supply clues for further interpretation about the

关键词： pI5 CKI tumor suppressor gene cell cycle induction of tumor cell differentiation.

Biodegradation of Leather Waste by Enzymatic Treatment

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过程工程学报 2006年第3期6卷 462-465页

作者： Muhammad Nauman Aftab Abdul Hameed Ikram-ul-Haq 陈润生 Dept. biol. Sci. Quaid-i-Azam Univ.IslamabadPakistan Bioinformatics LabInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate University of Chinese Academy of SciencesBeijing 100049China Dept. biol. Sci. Quaid-i-Azam Univ.IslamabadPakistan Dept. Botany Government College Univ.LahorePakistan Bioinformatics lab Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

The treatment of shavings,trimmings and splits of leather waste from tanneries has a potential to generate value-added products. In this study enzymatic treatment of leather waste was performed. This method utilizes alkaline protease produced by Bacillus subtilis in our lab.ratory by submerged fermentation. Optimum conditions of pH,time duration,temperature and concentration of enzyme were determined for maximum degradation of leather waste. The amount of degradation was measured by the release of amino acid hydroxyproline. Amino acid composition in the hydrolysate obtained by the enzyme hydrolysis was determined. This relative simple biotreatment of leather waste may provide a practical and economical solution.

关键词： leather waste biodegadation Bacillus subtilis alkaline protease

Effects of all-trans retinoic acid on proliferation of human aorta smooth muscle cells

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Chinese Science Bulletin 1999年第5期44卷 448-451页

作者： Guoxia Liu Cunxi Li Lingfang Zeng Yongchao Wang Beijing Normal Univ Key Lab Cell Proliferat & Regulat Biol Beijing 100875 Peoples R China

The primary cultured human aorta smooth muscle cells(SMCs)were characterized and theeffects of all—trans retinoic acid(atRA)on these cells were *** is found that atRA could slowdown the growth rate of SMCs,and decrease the expression Ievels of cyclinDl,CDK4,c-myc at39％。38％and 43.6％respectively,while atRA had no effects on the expression of c^-actin which isspecific for *** demonstrated that atRA inhibited cell growth of SMCs but did not induce *** effects may be clue to the decreased translation Ievels of ***4 andtranscription Ievel of *** fact that ati tA inhibited SMCs proIiferation may become one of thetheoretic basis for the cIinicaI use of atRA in the trea㈣t of SMCs proliferative diseases such asatherosclerosis.

关键词： SMC RA proliferation inhibition.

Interaction between DREF and E2F in transcriptional regulation of Drosophila genes for DNA replication

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Interaction between DREF and E2F in transcriptional regulati...

Rapid fabrication of modular 3D paper-based microfluidic chips using projection-based 3D printing

Third Congress of the Asian-Pacific Organization for cell biol.gy

作者： Akio Matsukage Fumiko Hirose Tomoyuki Sawado Masamitsu Yamaguchi lab. cell biol. Aichi Cancer Center Res. Inst.KanokodenChikusa-ku Nagoya 464-8681Japan

One of the most important endopoints of growth signal transduction cascades and cell cycle regulation mechanisms is transcriptional activation of the genes essential for cell proliferation. In studies on the regulation of Drosophila DNA replication-related genes, we found that a novel regulatory element consisting

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BIO-DESIGN AND MANUFACTURING 2024年第5期7卷 611-623页

作者： Xie, Mingjun Fu, Zexin Lu, Chunfei Wu, Sufan Pan, Lei He, Yong Sun, Yi Wang, Ji Hangzhou Med Coll Zhejiang Prov Peoples Hosp Affiliated Peoples Hosp Plast & Reconstruct Surg CtrDept Plast & Reconstr Hangzhou 310014 Peoples R China Zhejiang Univ Sch Mech Engn State Key Lab Fluid Power & Mechatron Syst Hangzhou 310058 Peoples R China Zhengzhou Univ Key Lab Mat Proc & Mold Zhengzhou 450002 Peoples R China Zhejiang Univ Coll Mech Engn Key Lab 3D Printing Proc & Equipment Zhejiang Prov Hangzhou 310058 Peoples R China Zhejiang Univ Canc Ctr Hangzhou 310058 Peoples R China

Paper-based microchips have different advantages, such as better biocompatibility, simple production, and easy handling, making them promising candidates for clinical diagnosis and other fields. This study describes a method developed to fabricate modular three-dimensional (3D) paper-based microfluidic chips based on projection-based 3D printing (PBP) technology. A series of two-dimensional (2D) paper-based microfluidic modules was designed and fabricated. After evaluating the effect of exposure time on the accuracy of the flow channel, the resolution of this channel was experimentally analyzed. Furthermore, several 3D paper-based microfluidic chips were assembled based on the 2D ones using different methods, with good channel connectivity. Scaffold-based 2D and hydrogel-based 3D cell culture systems based on 3D paper-based microfluidic chips were verified to be feasible. Furthermore, by combining extrusion 3D bioprinting technology and the proposed 3D paper-based microfluidic chips, multiorgan microfluidic chips were established by directly printing 3D hydrogel structures on 3D paper-based microfluidic chips, confirming that the prepared modular 3D paper-based microfluidic chip is potentially applicable in various biomedical applications. [GRAPHICS] .

关键词： Paper-based microfluidic chip Projection-based 3D printing (PBP) Modularization cell culture LOW-COST DEVICES WAX