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Surface-confined persistent luminescence with amplified FRET efficiency for cell-cell communication monitoring

Science China Chemistry 2025年

作者： Ziyun Miao Wenjing Dai Yubin Jin Bing Qi Jiayi Chen Lingling Qiu Yurong Wei Jie Wang The Key lab of Health Chemistry&Molecular Diagnosis of Suzhou College of ChemistryChemical Engineering&Materials ScienceSoochow University

Microbial communication monitoring is vital for understanding the mechanisms beneath gut microbiota equilibrium, infections,and biomanufacturing. Herein, we reported monitoring bacterial communication by surface-confined persistent ***, we constructed core-shell structured Zn1.4Ga1.2Ge0.4O4@Zn1.4Ga1.2Ge0.4O4:Cr（ZGGO@ZGGO:Cr） persistent luminescence nanoparticles（PLNPs） with amplified FRET efficiency for the detection of azoreductase（AzoR） that participates in bacterial communication. ZGGO@ZGGO:Cr nanoparticles were functionalized with an azo black hole quencher（ZGGO@ZGGO:Cr-BHQ-3）. Due to the proximity of surface-confined persistent luminescence with the surface-bound BHQ-3,ZGGO@ZGGO:Cr-BHQ-3 exhibited a FRET efficiency up to 1.6 times that of traditional ZGGO:Cr-BHQ-3. Further, the specific catalytic reduction of azo bonds in BHQ-3 by bacterial extracellular AzoR recovers the persistent luminescence of ZGGO@ZGGO:Cr. ZGGO@ZGGO:Cr-BHQ-3 displayed superior sensitivity and speed in detecting extracellular AzoR over ZGGO:Cr-BHQ-3, attributed to its surface-confined persistent luminescence. We found that the peak secretion of AzoR occurs during the exponential growth period of bacteria including S. aureus and E. coli based on the persistent luminescence recovery rate of ZGGO@ZGGO:Cr-BHQ-3. Furthermore, ZGGO@ZGGO:Cr-BHQ-3 was used to probe the communication between different bacterial species. R. palustris is a kind of lycopene-production bacteria. We found that the cohabitation of R. palustris and S. putrefaciens boosted both AzoR secretion and lycopene production. Moreover, the secretion and activity of AzoR positively correlated with lycopene production, suggesting that AzoR may participate in the communication between R. palustris and S. putrefaciens to promote lycopene production. This study offers a robust method to enhance PLNP-based biosensing sensitivity and provides a promising strategy for microbial communication monitoring.

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第01期 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay laboratory State Key laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of chemical biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

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Dielectric-ion-conductive ZnNb2O6 layer enabling rapid desolvation and diffusion for dendrite-free Zn metal batteries

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Journal of Energy Chemistry 2025年第1期100卷 693-701页

作者： Haifeng Yang Jian Wang Panpan Zhang Xiaomin Cheng Qinghua Guan Jing Dong Bixian Chen Lujie Jia Jing Zhang Yongzheng Zhang Yunjian Liu Hongzhen Lin School of Materials Science and Engineering Jiangsu University i-lab & CAS Key laboratory of Nanophotonic Materials and Devices Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences Helmholtz Institute Ulm (HIU) Karlsruhe Institute of Technology (KIT) School of Materials Science and Engineering Xi'an University of Technology State Key laboratory of chemical Engineering East China University of Science and Technology

Rechargeable aqueous zinc-metal batteries （AZMBs） are promising candidates for large-scale energy storage systems due to their low cost and high ***,their performance and sustainability are significantly hindered by the sluggish desolvation kinetics at the electrode/electrolyte interface and the corresponding hydrogen evolution reaction where active water molecules tightly participate in the Zn（H2O）62+solvation ***,learnt from self-generated solid electrolyte interphase （SEI） in anodes,the dielectric but ion-conductive zinc niobate nanoparticles artificial layer is constructed on metallic Zn surface （ZNB@Zn）,acting as a rapid desolvation *** zincophilic and dielectric-conductive properties of ZNB layer accelerate interfacial desolvation/diffusion and suppress surface corrosion or dendrite formation,achieving uniform Zn plating/stripping behavior,as confirmed by electronic/optical microscopies and interface spectroscopical measurements together with theoretical ***,the as-prepared ZNB@Zn electrode exhibits excellent cycling stability of over 2000 h and robust reversibility （99.54%） even under high current density and depth of discharge ***,the assembled ZNB@Zn-based full cell displays high capacity-retention rate of 80.21%after 3000 cycles at 5 A g-1and outstanding rate performance up to 10 A *** large-areal pouch cell is stabilized for hundreds of cycles,highlighting the bright prospects of the dielectric but ion-conductive layer in further application of AZMBs.

关键词： Zn metal battery Dielectric artificial layer Rapid ion diffusion Zincophilic diffusion Active water inhibition

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双激发驱动的全组分响应镧系金属-有机骨架用于多元疾病标志物的双模式检测（英文）

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Science China Materials 2025年

作者：陈国礼代斌郝继娜李永生 lab of Low-Dimensional Materials Chemistry Key Laboratory for Ultrafine Materials of Ministry of Education Frontier Science Center of the Materials Biology and Dynamic Chemistry Shanghai Engineering Research Center of Hierarchical Nanomaterials School of Materials Science and Engineering East China University of Science and Technology School of Chemistry and chemical Engineering State Key Laboratory Incubation Base for Green Processing of Chemical Engineering Shihezi University

检测多种小分子代谢标志物比生物大分子更能灵敏、实时地反馈生理或病理状态,但因其缺乏特定识别基团,使其精准检测更具挑战性.现有荧光探针大多仅在单一激发波长下对不同分析物产生类似的输出且含非功能组分,难以同时准确检测多个小分...

检测多种小分子代谢标志物比生物大分子更能灵敏、实时地反馈生理或病理状态,但因其缺乏特定识别基团,使其精准检测更具挑战性.现有荧光探针大多仅在单一激发波长下对不同分析物产生类似的输出且含非功能组分,难以同时准确检测多个小分子标志物.针对此,我们充分利用MOFs有机和无机构建单元,导向设计了一种双激发驱动、全组分响应的MOF荧光探针（CeTMA-TMA-Eu）,分别在262和296 nm激发下实现了对疾病标志物假尿苷（Ψ,与TMA配体作用过滤262 nm激发光）和N-乙酰天冬氨酸(NAA,诱导Ce4+节点价态变化)的特异荧光猝灭和增强响应,其检出限低至0.16和0.15μM,检测范围分别为0–180和0–100μM.该探针减少了与非目标分子的相互作用,显著提高了探针区分各目标物的选择性和分辨率.

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Effect of arginine on stability of GST-ZNF191(243-368)

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Chinese chemical Letters 2007年第3期18卷 355-356页

作者： Dong Xin Zhao Zhong Xian Huang chemical biology lab Department of Chemistry Fudan University Shanghai 200433 China

For better understanding the chemical or biological information of ZNF191 （243-368）, we expressed the fusion protein of GST and ZNF191 （243-368）, and used it to obtain the binding DNA sequence of this zinc finger protein. But in the process of expression and purification, we found this fusion protein slowly degradated. For resolving this problem, we simultaneously added charged amino acids L-Arg and L-Glu to the solution of fusion protein, and demonstrated that this method can dramatically increase the stability of this fusion protein. This method can make the fusion protein suitable for the continuous works, especially for situations where high protein concentration and long-term stability without precipitate and degradation of protein are required.

关键词： ZNF191(243-368) GST Stability L-Arg L-Glu

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Spin-modulated near-infrared-Ⅰ emission in a xanthene-decorated Fe(Ⅱ) spin-crossover complex

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Science China Chemistry 2025年

作者： Cheng Yi Duyong Chen Shihui Zhang Rui Cai Chunying Duan Yinshan Meng Tao Liu State Key laboratory of Fine chemicals Frontier Science Center for Smart Materials and Chemical Engineering School of Chemical Engineering Dalian University of Technology Instrumental Analysis Center Dalian University of Technology School of Chemistry Dalian University of Technology Liaoning Binhai laboratory

Molecular materials showing synergetic coupling of near-infrared （NIR） fluorescence and spin-crossover （SCO） are promisingly applicable in in-vivo bioimaging,temperature sensing,and spintronic *** the related study faces formidable *** this work we graft the NIR fluorophore xanthene onto the dipyridyl substituted triazole ligand and construct a new Febased SCO material,{FeⅡ（ddxc-abpt）2[N（CN）2]2}(1,ddxc-abpt:（E）-4-(((3,5-di（pyridin-2-yl）-4H-1,2,4-triazol-4-yl)imino)methyl)-N,N-diethyl-2,3-dihydro-1H-xanthen-6-amine).Magnetic studies indicate that compound 1 exhibits thermally induced SCO property in both the solid and solution ***-temperature fluorescence emission spectra reveal a remarkable 7-fold increase in photoluminescence intensity upon the transition from the low-spin state at 110 K to the high-spin state at 280 K,which is the record of all reported coupled fluorescence-SCO ***-temperature ultraviolet-visible （UV-Vis）absorption spectra show a significant change in absorption intensity within the fluorescence emission window,consistent with the F?rster resonance energy transfer （FRET） ***-dependent density functional theory （TD-DFT） calculations demonstrate that the strong coupling is attributed to disrupted energy transfer between the xanthene fluorophore and high-spin *** work provides a feasible approach for expanding the synergetic SCO-fluorescence materials to the NIR-Iwavelength region,and hopefully contributes to the applications of tunable NIR-SCO molecule-based sensors and devices.

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Anti-proliferative potential of sodium thiosulfate against HT 29 human colon cancer cells with augmented effect in the presence of mitochondrial electron transport chain inhibitors

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Asian Pacific Journal of Tropical Biomedicine 2020年第7期10卷 333-340页

作者： Bhavana Sivakumar Sri Rahavi Boovarahan Gino A Kurian Vascular biology lab School of Chemical and BiotechnologySASTRA Deemed UniversityThanjavurIndia

Objective:To compare the anti-proliferative effect of sodium thiosulfate on human colorectal cancer cells(HT-29)and normal small intestine cells(IEC6).Methods:Cells(HT-29 and IEC6)were treated with different concentrations of sodium thiosulfate ranging from 0.5 m M to 80 m M for 24 *** viability was measured via crystal violet and MTT ***-29 cells were further treated in the presence and absence of mitochondrial electron transport chain(ETC)inhibitors,KATP channel opener and closer and H2S inhibitors for 24 h followed by sodium thiosulfate in order to study their respective roles in the anti-proliferative activity of sodium ***:The IC50 values of sodium thiosulfate on HT-29 cells were 40.93 m M and 42.45 m M by crystal violet and MTT assay whereas,in the case of IEC6 cells,the values were 45.17 m M and 47.22 m *** inhibition of endogenous H2S enzymes and KATP channel induced no change in the anti-proliferative capacity of sodium ***,the anti-proliferative activity of sodium thiosulfate was enhanced in the presence of mitochondrial ETC ***:HT-29 cell growth is effectively attenuated by sodium thiosulfate and the anti-proliferative activity of sodium thiosulfate is enhanced in the presence of mitochondrial ETC inhibitors.

关键词： Colorectal cancer Sodium thiosulfate HT-29 IEC6 Mitochondria Rotenone Clonogenic assay

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New progress in active immunotherapy targeting to amyloid beta

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Science China Chemistry 2015年第3期58卷 383-389页

作者： Gao Li Yong-Xiang Chen Yan-Mei Li Key lab of Bioorganic Phosphorus Chemistry and chemical biology Ministry of Education Department of Chemistry Tsinghua University Beijing Institute for Brain Disorders

Alzheimer's disease(AD) is one of the most common types of dementia whose hallmarks include neurofibrillary tangles and senile plaques. The latter are mainly composed of amyloid-β proteins(Aβ), and it's suggested that Aβ may be the causative factor in AD pathogenesis. Immunotherapy targeting Aβ for preventing aggregation of Aβ and mildly clearing amyloid plaques has been a hot topic since 1999. Although the first clinical trial of Aβ vaccine, AN-1792, failed in phase II, its results suggested some key points in the design of Aβ vaccines. Avoiding the possible toxic Aβ specific T cell response and inducing a Th2 type cellular immune response may be beneficial for Aβ immunotherapy. Many associations and research groups are working on Aβ vaccine and some progress has been made in recent years. In this review, we have provided a detailed summary of past Aβ vaccines, which have been sorted by the immunogen, and we also discuss some recent progress and future perspectives.

关键词： Alzheimer's disease amyloid beta active immunotherapy peptide vaccine DNA vaccine

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Syntheses, Crystal Structures and Antifungal Activities of Two Compounds Containing Pyrazole and Pyrethroid Units

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Chinese Journal of Structural Chemistry 2015年第8期34卷 1231-1237页

作者： ZHOU Bao-Han HUA Yun-Tao XU Bao-Ming MA Li HU Jia-Wen LIU Zhao-Jie School of Chemistry and chemical Engineering Light Industry Division Hubei University of Technology Key lab of Pesticide and chemical biology Ministry of EducationCentral China Normal University

Compounds 1 （C15H17C1F3N303, Mr = 379） and 2 （C14H14C1F3N4OS, Mr = 378） have been synthesized and their crystal structures were determined by single-crystal X-ray diffraction. Crystal 1 belongs to the triclinic system, space group P1 with a = 6.0223（19）, b = 9.324（3）, c = 15.936（5） A, a = 80.687（5）, β= 87.289（5）, ）, = 86.097（5）°, V= 880.4（5） A3, Z = 2, Dc = 1.433 g/cm3,μ（MoKa） = 0.266 mm^-1, F（000） = 392, R = 0.0861 and wR = 0.1999 for 2022 observed reflections with I 〉 2o（/）. Crystal 2 belongs to the triclinic system, space group PI with a = 7.7029（15）, b = 8.3371（16）, c = 14.410（3） A, a = 100.672（3）, β= 103.168（3）, ？ = 98.726（3）°, V = 876.1（3） A3, Z = 2, Dc= 1.451 g/cm^3,μ（MoKa） = 0.379 mm^-1, F（000） = 388, R = 0.0672 and wR = 0.2105 for 2725 observed reflections with I 〉 2σ（I）. Although the two compounds are similar with the same pyrazole and pyrethroid units, X-ray analysis reveals that their structures are completely different.

关键词： pyrazole, crystal structures, synthesis, antifungal activities

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Penta-coordinated phosphorus structure analysis on kinases

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Science China Chemistry 2004年第5期47卷 420-427页

作者： LI Wu MA Yuan ZHAO Yufen Key lab of Bioorganic Phosphorus Chemistry and chemical biology of Ministry of Education Department of Chemistry School of Life Sciences Tsinghua University Beijing 100084 China Key Lab of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education Department of Chemistry School of Life Sciences Tsinghua University Beijing 100084 China Key Lab of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education Department of Chemistry School of Life Sciences Tsinghua University Beijing 100084 China

In this paper, based on the known crystal structures of Square Pyramid (SP) and Trigonal Bipyramid (TBP) penta-coordinated phosphorus compounds containing amino acid side chains, such as amino, carboxyl, hydroxyl or thiol, a software designed to survey the P(5)-Structure of phosphorylated proteins. By this software, it was found that 382 of 398 phos- phorus-related kinases (96%) from current PDB could go through the penta-coordinated phos- phorus transition state or intermediate. For example, in protein 1HE8, amino group in Lysine16 was within 3.58 ?from the phosphorus atom, and a potential TBP structure consisting GNP2 and NZ could be overlapped with an authentic TBP with an RMSD value of 0.71 ?.

关键词： penta-coordinated phosphorus structures, kinases.

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