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Astrocyte reactivity in related brain regions in a mouse model of MPTP-induced Parkinson's disease

Neural Regeneration Research 2009年第2期4卷 129-134页

作者： Zhijun Zhang Chunlin Xia Yulin Dong Guangming Lü Juan Liu Lin Ding Hengjian Ni Department of Human Anatomy Institute of Neurobiology Jiangsu Province Key Laboratory of Neural Regeneration Medical School of Nantong University Nantong 226001 Jiangsu Province China lab of aging and nervous disease & cytoneurobiology unit Soochow University Suzhou 215123 Jiangsu Province China

BACKGROUND： Severe injury to dopaminergic neuronal cell bodies and their axon terminals in the substantia nigra pars compacta （SNC） has been observed in both Parkinson＇s disease （PD） patients or in 1-methy-4-phenyl-1,2,3,6-tetrahydropyrindine（MPTP）-induced PD animal models, but only slight injury occurs in the adjacent ventral tegmentat area （VTA）. The mechanisms underlying this selective injury remain poorly understood. OBJECTIVE： To comparatively observe astrocyte reactivity in the SNC, caudate putamen （CPu）, VTA, and frontal association cortex （FrA）. DESIGN, TIME AND SETTING： A cellular and molecular biology, randomized, controlled experiment was performed at the Institute of Neurobiology, Department of Human Anatomy, Medical School of Nantong University, between December 2006 and September 2008. MATERIALS： A total of 80 healthy adult male C57BL/6 mice were included in this study. MPTP was purchased from Sigma, USA. METHODS： Mice were randomly divided into a model group （n = 64） and a sham-operated group （n = 16）. PD was induced in the mice from the model group by intraperitoneal injection of 20 mg/kg MPTP, once every three hours, for a total of 4 times. MAIN OUTCOME MEASURES： Tyrosine hydroxylase （TH）-immunoreactive neurons and glial fibrillary acidic protein （GFAP）-immunoreactive astrocytes were examined by dual immunofluorescence labeling. GFAP-immunoreactive astrocytes in the CPu and FrA were determined by immunofluorescent staining. GFAP mRNA expression in the SNC, CPu, VTA, and FrA was detected using real-time polymerase chain reaction. TH protein levels in the TH-immunoreactive axon terminals of the CPu and FrA were detected by Western blotting. RESULTS： Numbers of TH-immunoreactive neurons in the SNC, and TH protein level in the CPu, markedly decreased （by approximately 68%） 1 day after MPTP injection, and gradually increased at 3 days. Simultaneously, astrocyte reactivity was strengthened, in particular at 7 days. However, after MPTP injection,

关键词： Parkinson's disease 1-methy-4-phenyl-1, 2, 3, 6-tetrahydropyrindine dopaminergic neuron astrocyte mouse

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Involvement of nitrergic neurons in colonic motility in a rat model of ulcerative colitis

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World Journal of Gastroenterology 2022年第29期28卷 3854-3868页

作者： Yan-Rong Li Yan Li Yuan Jin Mang Xu Hong-Wei Fan Qian Zhang Guo-He Tan Jing Chen Yun-Qing Li Department of Human Anatomy Basic Medical CollegeGuangxi Medical UniversityNanning 530000Guangxi Zhuang Autonomous RegionChina Department of Gastroenterology The First Affiliated Hospital of Jinzhou Medical UniversityJinzhou 121000Liaoning ProvinceChina Department of Human Anatomy Basic Medical CollegeZunyi Medical UniversityZunyi 563006Guizhou ProvinceChina Department of Anatomy Basic Medical CollegeDali UniversityDali 671000Yunnan ProvinceChina Department of Anatomy Histology and EmbryologyXuzhou Medical UniversityXuzhou 221000Jiangsu ProvinceChina Key lab of Longevity and aging-related diseases of Chinese Ministry of Education Guangxi Medical UniversityNanning 530000Guangxi Zhuang Autonomous RegionChina School of Basic Medical Sciences and Center for Translational Medicine Guangxi Medical UniversityNanning 530000Guangxi Zhuang Autonomous RegionChina Department of Anatomy Histology and Embryology and K.K.Leung Brain Research CentreThe Fourth Military Medical UniversityXi'an 710032Shaanxi ProvinceChina Department of Human Anatomy College of Preclinical Medical SciencesZhengzhou UniversityZhengzhou 450001Henan ProvinceChina Key laboratory of Brain Science Research and Transformation in Tropical Environment of Hainan Province Hainan Medical UniversityHaikou 571199Hainan ProvinceChina

BACKGROUND The mechanisms underlying gastrointestinal(GI)dysmotility with ulcerative colitis(UC)have not been fully *** enteric nervous system(ENS)plays an essential role in the GI *** a vital neurotransmitter in the ENS,the gas neurotransmitter nitric oxide(NO)may impact the colonic *** this study,dextran sulfate sodium(DSS)-induced UC rat model was used for investigating the effects of NO by examining the effects of rate-limiting enzyme nitric oxide synthase(NOS)changes on the colonic motility as well as the role of the ENS in the colonic motility during *** To reveal the relationship between the effects of NOS expression changes in NOS-containing nitrergic neurons and the colonic motility in a rat UC *** Male rats(n=8/each group)were randomly divided into a control(CG),a UC group(EG1),a UC+thrombin derived polypeptide 508 trifluoroacetic acid(TP508TFA;an NOS agonist)group(EG2),and a UC+NG-monomethyl-L-arginine monoacetate(L-NMMA;an NOS inhibitor)group(EG3).UC was induced by administering 5.5%DSS in drinking water without any other treatment(EG1),while the EG2 and EG3 were gavaged with TP508 TFA and L-NMMA,*** disease activity index(DAI)and histological assessment were recorded for each group,whereas the changes in the proportion of colonic nitrergic neurons were counted using immunofluorescence histochemical staining,Western blot,and enzyme linked immunosorbent assay,*** addition,the contractile tension changes in the circular and longitudinal muscles of the rat colon were investigated in vitro using an organ bath *** The proportion of NOS-positive neurons within the colonic myenteric plexus(MP),the relative expression of NOS,and the NOS concentration in serum and colonic tissues were significantly elevated in EG1,EG2,and EG3 compared with CG *** UC rats,stimulation with agonists and inhibitors led to variable degrees of increase or decrease for each indicator in the EG2 and ***

关键词： Nitrergic neurons Nitric oxide Nitric oxide synthase Ulcerative colitis Colonic motility Colonic myenteric plexus

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