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Journal of Genetics and Genomics 2015年第9期42卷 507-510页

作者： Zhou Tan Zhongkai Hu Emily Y.Cai Cantas Alev Ting Yang Zhen Li Joyce Sung Yasser Yehia El-Sayed Gary M.Shaw David K.Stevenson Atul J.Butte Guojun Sheng Karl G.Sylvester Harvey J.Cohen Xuefeng B.Ling Institute of Developmental and Regenerative Biology Hangzhou Normal University Department of Surgery Stanford University lab for early embryogenesis RIKEN Center for Developmental Biology Department of Obstetrics and Gynecology Stanford University Department of Pediatrics Stanford University Stanford

Over 11% of all pregnancies in the US result in preterm birth, greatly contributing to perinatal morbidity and mortality （Goldenberg and Rouse, 1998）. Preterm birth etiologies remain largely unknown, and effective prevention methods have yet to be developed. The use of biofluid （e.g., serum or urine） for the analysis of the naturally occurring peptidome （MW 〈 4000） as a source of biomarkers has been reported for different diseases （Villanueva et al., 2006; Ling et al., 2010a, 2010b, 2010c, 2011）. Mass spectrometry-based profiling of naturally occurring peptides can provide an extensive in- ventory of serum peptides derived from either high-abundant endogenous circulating proteins or cell and tissue proteins （Liotta and Petricoin, 2006）.

关键词： Serological Targeted Analysis of an ITIH4 Peptide Isoform A Preterm Birth Biomarker and Its Associated SNP Implications SNP

Unveiling the morphogenetic code:A new path at the intersection of physical energies and chemical signaling

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World Journal of Stem Cells 2021年第10期13卷 1382-1393页

作者： Riccardo Tassinari Claudia Cavallini Elena Olivi Valentina Taglioli Chiara Zannini Carlo Ventura National laboratory of Molecular Biology and Stem Cell Engineering National Institute of Biostructures and Biosystems–ELDOR LABBologna 40129Italy

In this editorial,we discuss the remarkable role of physical energies in the control of cell signaling networks and in the specification of the architectural plan of both somatic and stem *** particular,we focus on the biological relevance of bioelectricity in the pattern control that orchestrates both developmental and regenerative *** this end,the narrative starts from the dawn of the first studies on animal electricity,reconsidering the pioneer work of Harold Saxton Burr in the light of the current *** finally discuss the most recent evidence showing that bioelectric signaling is an essential component of the informational processes that control pattern specification during embryogenesis,regeneration,or even malignant *** conclude that there is now mounting evidence for the existence of a Morphogenetic Code,and that deciphering this code may lead to unprecedented opportunities for the development of novel paradigms of cure in regenerative and precision medicine.

关键词： Physical energies Stem cells Bioelectricity Electromagnetic radiation Mechanical forces Morphogenetic code

Time course of foreign gene integration and expression in transgenic fish embryos

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Chinese Science Bulletin 2000年第8期45卷 734-739页

作者： Haobin Zhao Shangping Chen Yonghua Sun Yaping Wang Zuoyan Zhu Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Wuhan 430072 Peoples R China

Using a nuclear transplantation approach, the integration and expression of the green fluorescent protein (GFP) gene in the embryogenesis of transgenic loach (Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are co-injected into fertilized eggs, the behavior of their integration and expression is not identical.

关键词： gene integration and expression time course nuclear transplantation fish GFP.

Reproductive polyphenism and its advantages in aphids:Switching between sexual and asexual reproduction

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Journal of Integrative Agriculture 2020年第6期19卷 1447-1457页

作者： YAN Shuo WANG Wan-xing SHEN Jie Department of Entomology/MOA Key lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijing 100193P.R.China Key lab of Biology and Genetic Improvement of Root and Tuber Crops Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Reproductive polyphenism,which allows one genotype to produce sexual and asexual morphs,is an extreme case of phenotypic plasticity and is commonly observed in *** are typical species that switch these reproductive modes,and the pathway orientation is triggered by the environmental conditions(mainly photoperiod and temperature).The typical arm ual life of aphids in eludes a successi on of parthenogenetic gen erations duri ng the spri ng and summer and a single sexual generation in *** this review,we describe how the environmental cues orientate the reproductive mode of aphids from photoperiodic perception to endocrine regulation,and how juvenile hormones may act on the target cells(oocytes)to initiate the gametogenesis and embryogenesis in sexual and asexual *** also discuss the paradox of sex,especially the advantages of sexual reproduction in *** the recent development of genomic resources in aphids,many potential genes involved in the reproductive polyphenism will enter the public's *** particular,we describe a novel RNAi method in aphids,which may provide a molecular technique for determining the developmental fate and multiple reproductive strategies.

关键词： aphids parthenogenesis embryogenesis gametogenesis photoperiodism

Characterization of SoxB2 and SoxC genes in Amphi-oxus (Branchiostoma belcheri):Implications for their evolutionary conservation

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Science China(Life Sciences) 2009年第9期52卷 813-822页

作者： LIN YuShuang,CHEN DongYan,FAN QiuSheng & ZHANG HongWei Institute of Developmental Biology,Life Science College,Key lab of Experimental Teratology of Ministry of Education,Shandong University,Jinan 250100,China Institute of Developmental Biology Life Science College Key Lab of Experimental Teratology of Ministry of Education Shandong University Jinan China

Most Sox genes directly affect cell fate determination and differentiation. In this study,we isolated two Sox genes:SoxB2 and SoxC from amphioxus (Branchiostoma belcheri),the closest living invertebrate relative of the vertebrates. Alignments of SoxB2 and SoxC protein sequences and their vertebrate homologs show high conservation of their HMG domains. Phylogenic analysis shows that amphioxus SoxB2 and SoxC fall out of the vertebrate branches,suggesting that vertebrate homologs might arise from gene duplications during evolution. The two genes possess similar spatial and temporal expression patterns during embryogenesis and in adults. They are both maternally inherited. During neurulation,they are expressed in the neural ectoderm and archenterons. In adults,they are expressed not only in the nerve cord,but also in the gut,midgut diverticulum,gill and oocytes. These results suggest that amphioxus SoxB2 and SoxC might co-function and have conserved functions in the nervous system and gonads as their vertebrate homologs.

关键词： amphioxus SoxB2 SoxC neurulation gonads immunity evolution

Angiogenesis in female reproductive system

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Chinese Science Bulletin 2001年第14期46卷 1145-1148页

作者： Longjiang Shao Cheng Zhu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

Neovascularization, i.e. new blood vessels formation, can be divided into two different processes: vasculogene-sis, whereby a primitive vascular network is established during embryogenesis from multipotential mesenchymal progenitors; and angiogenesis, which refers to the new blood vessels formation from pre-existing vessels[1,2].Angiogenesis contributes to the most process throughout the whole life span from embryonic development to adult growth’21. In this meaning, neovascularization is usually used to imply angiogenesis. Under physiological conditions, angiogenesis is a strictly regulated event and rarely happens in most adult tissues except for fracture or healing of wounds[2,3]. However, a notable phenomenon is that the tissues of ovary and uterine endometnum are unique in the cycle-specific changes in vascularity that occur in each estrous/menstrual cycle. Active angiogenesis occurs in placenta to satisfy the needs of embryonic implantation and development. Defects in angiogenesis are associated

关键词： Angiogenesis in female reproductive system

Expression of vascular endo-thelial growth factor in rat uterus during peri-implantation

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Chinese Science Bulletin 2001年第14期46卷 1178-1182,1234页

作者： Longjiang Shao Hongmei Wang Enkui Duan Cheng Zhu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

The first distinct mark of rodent implantation is the increased vascular permeability and significant angio-genesis at the sites of blastocyst implantation, but its mechanism is not clearly defined. Vascular endothelial growth factor (VEGF) is the key mediator for angiogenesis during em-bryogenesis and adult span and also serves as a vascular permeability factor. The aim of this study is to explore VEGF regulation mechanism and the possible role that VEGF plays in implantation by studying the VEGF expression and angiogenesis in the rat uterus during estrous cycle, ovarioec-tomized and peri-implantation stages using in situ message RNA hybridization and confocal laser scanning techniques. The results indicated that VEGF was regulated by ovarian steroid hormones. VEGF expression before implantation was localized at luminal epithelium, shifted to stroma as implantation initiated and extensively located at the decidualizing stroma region after implantation. Bandeiraea simplicifolia-1 (BS-1) agglutinin and

关键词： rat vascular endothelial growth factor embryo implantation angiogenesis.

A Study on the Sensibility of Embryogenic Suspension Cells of Anthurium andraeanum to Two Antibiotics

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Agricultural Biotechnology 2015年第2期4卷 8-11页

作者： Bo YU Genfa ZHU Yingbo SUN Environmental Horticulture Institute Guangdong Academy of Agricultural Sciences / Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization

In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates,somatic embryogenesis and plantlet regeneration of *** to the results,at the embryonic cell propagation stage,lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of *** were 200 and 60 mg/L,respectively;at the differentiation stage,either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis;either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet *** results provided important reference for further studies of transgenic ***.

关键词： Anthurium embryogenesis somatic transgenic regeneration embryonic suspension Araceae callus breeding

PPR17, PPR20 and CRS1 mediate intron splicing by forming a complex in maize chloroplasts

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PPR17, PPR20 and CRS1 mediate intron splicing by forming a c...

Functional genomics in maize seed development

2016年全国植物生物学大会

作者： Rui Liu Xiaoyan Zhang Shikai Cao Bao-Cai Tan Key lab of Plant Cell Engineering and Germplasm Innovation Ministry of EducationSchool of Life SciencesShandong University

In mitochondria and chloroplasts of higher plants,the loss of self-splicing activity of degenerate group II introns results in recruitment of intron splicing factors encoded in the nucleus. One family of such factors is the pentatricopeptide repeat proteins(PPR) which are implicated in editing,splicing,end maturation and regulation of translation in organellar transcripts. PPR proteins belong to a large family with more than 500 members in flowering plants. Because of its large size and frequent embryo lethality in KO mutants,functions of many PPR proteins remain unknown. Here we report the function identification of PPR17 and PPR20 and their physical interactions with Chloroplast RNA Spl icing 1(CRS1) in maize. Null mutation of PPR17 and PPR20 showed similar arrest in embryogenesis at the transition stage. Both PPR17 and PPR20 are P-type PPR proteins with 11 and 17 PPR motifs respectively. Subcellular localization revealed that both proteins are localized in the chloroplast. PPR17 and PPR20 are expressed in all the tissues examined. Analysis of chloroplast transcripts revealed that loss of either the PPR17 or the PPR20 function abolishes the splicing of the atp F and rpl2 introns,suggesting that PPR17 and PPR20 are required for atp F and rpl2 intron splicing. Previous studies have identified that CRM protein CRS1 is also required for atp F intron splicing(Jenkins et al.,1997;Till et al.,2001). Yeast two hybridization,Pull-down and Bi FC analyses indicated that PPR17,PPR20 and CRS1 interact physically. Domain deletion analysis showed that the first four PPR motifs of PPR17 interact with the first two CRM domains of CRS1 and PPR20,and the C-terminal region of CRS1 interacts with PPR20. These results demonstrate that PPR17,PPR20 and CRS1 mediate the atp F intron splicing by forming a complex in maize chloroplasts.

关键词： PPR intron splicing chloroplast embryo and endosperm maize

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Functional genomics in maize seed development