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Acaricidal activity of derris floribunda essential oil and its main constituent

Asian Pacific Journal of Tropical Biomedicine 2017年第9期7卷 791-796页

作者： Ana Claudia Fernandes Amaral Aline de SRamos Marcia Reis Pena Jose Luiz Pinto Ferreira Jean Michel SMenezes Geraldo JNVasconcelos Neliton Marques da Silva Jefferson Rocha de Andrade Silva lab de Plantas Medicinais e derivados Depto de Quimica de Produtos NaturaisFarmanguinhosFundacao Oswaldo CruzRio de JaneiroRJBrazil lab de entomologia e acarologia agricola Faculdade de Ciencias AgrariasUniversidade Federal do AmazonasManausAMBrazil lab de Cromatografia Depto de QuímicaUniversidade Federal do AmazonasManausAMBrazil 4Instituto de Ciencias Exatas e TecnologiaUniversidade Federal do AmazonasItacoatiaraAMBrazil

Objective: To evaluate the acaricidal activity of the essential oil obtained from roots of derris floribunda(D. floribunda)(Miq.) Benth, and its main constituent nerolidol against the Mexican mite Tetranychus mexicanus(T. mexicanus)(Mc Gregor).Methods: The essential oil from the roots of D. floribunda collected in the Amazon region(Brazil) was obtained by hydrodistillation. Its chemical composition was determined by GC–MS analysis. The acaricidal activities of this essential oil and nerolidol,were evaluated by recording the number of dead females(mortality) and eggs(fertility).Results: The essential oil showed sesquiterpenes as major volatile components. Nerolidol, the main component, represented 68.5% of the total composition of the essential oil. D. floribunda essential oil and nerolidol showed acaricidal activity, with LC_(50) of9.61 mg/m L air and 9.2 mg/m L air, respectively, over a 72 h period. In addition, both the essential oil and nerolidol significantly reduced the fecundity of T. ***: Due to the economic importance of T. mexicanus and the lack of new pesticides, our data are very promising in the search for efficient and safer acaricidal products. Furthermore, this is the first report about the chemical composition and bioactivity of the essential oil of the Amazon plant species D. floribunda.

关键词： Acaricidal activity essential oil Nerolidol Organic agriculture Sesquiterpenes Timb

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Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs

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Insect Science 2020年第3期27卷 519-530页

作者： Silvia de Oliveira Dorta Juliana Balbinotte Rose Monnerat Joao Roberto Spotti Lopes Tatiane da Cunha Odimar Zanuzo Zanardi Marcelo Pedreira de Miranda Marcos Antonio Machado Juliana de Freitas-Astua Programs de Pos-Graduagao em Microbiologia agricola Escola Superior de Agricultura Luiz de QueirozUniversidade de Sao PauloPiracicabaSPBrazil laboratorio de Biotecnologia Centro de Citricultura Sylvio MoreiraInstituto Agronomico de CampinasCordeiropolisSPBrazil laboratorio de Insetos Vetores de Fitopatdgenos Departamento de Entomologia e AcarologiaEscola Superior de Agricultura Luiz de QueirozUniversidade de Sao PauloPiracicabaSPBrazil embrapa Recursos Geneticos e Biotecnologia BrasiliaDFBrazil Fundo de defesa da Citricultura(Fundecitrus) AraraquaraSPBrazil embrapa Mandioca e Fruticultura Cruz das AlmasBABrazil laboratdrio de Bioquimica Fitopatoldgica Instituto BioldgicoSao PauloSPBrazil

Bacillus thuringiensis(Bt)toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic *** discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap‐sucking insects,such as the Asian citrus psyllid Diaphorina citri Kuwayama(Hemiptera:Liviidae),a vector of“Candidatus Liberibacter spp.,”associated with citrus huanglongbing(HLB).In this study,translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil‐drench,and their systemic pathogenicity to *** nymphs were *** pathogenicity of three wild‐type Bt strains against *** third‐instar nymphs was *** the 10 recombinant strains tested(each of them harboring a single cry or cyt gene),3 can be highlighted,causing 42%–77%and 66%–90%nymphal mortality at 2 and 5 d after inoculation,*** isolation of Bt cells from young citrus shoots and dead nymphs,and PCR performed with specific primers,confirmed the involvement of the Bt strains in the psyllid *** is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control *** nymphs that fed on these soil‐drench inoculated *** Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control *** and the identified genes can be used for the production of transgenic Bt citrus.

关键词： Asian citrus psyllid Citrus sinensis Cry toxins endophytic Bt huanglongbing young shoots

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Spatial Statistics and Age Structure of Leaf Cutting Ant Nests

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Open Journal of Statistics 2019年第2期9卷 196-217页

作者： debora Maria Ferreira Canuto Sergio Furtado dos Reis Wesley Augusto Conde Godoy departamento de entomologia e acarologia Escola Superior de Agricultura Luiz de Queiroz Universidade de Sao Paulo Piracicaba Brazil departamento de Biologia Animal Universidade Estadual de Campinas Campinas Brazil

In this study, we investigated the spatial aggregation of old and incipient nests of Atta sexdens rubropilosa by fitting Poisson and Negative binomial models to nest abundance data. Our aim is to analyse the distribution of ant nests in eucalypt regrowth, Cerrado and native forest fragment. We also investigated the correlation between nest abundance and climatic factors, as well as different nest ages. When comparing nests of different ages we observed an aggregated pattern for both old and incipient nests. On the other hand, analysing the distribution of nests separately, only taking into account the different areas and respective borders, old nests exhibited an aggregated pattern and incipient nests showed a random pattern, except for native forest with ants exhibiting only an aggregated pattern. The levels of aggregation changed in response to different areas and border gradients, with more external borders showing higher aggregation than more internal borders. Temperature was the variable showing the highest correlation with nest abundance and the correlation between nests of different ages was totally depending on the different areas.

关键词： Spatial Statistics Leaf Cutting Ant Nest Aggregation Nest Age Forest Landscapes

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determining the Levels of Trace elements Cd, Cu, Pb and Zn in Honey of Stingless Bee (Hymenoptera: Apidae) Using Voltammetry

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Food and Nutrition Sciences 2015年第7期6卷 591-596页

作者： Andreia Santos do Nascimento Luis Carlos Marchini Carlos Alfredo Lopes de Carvalho Diogo Feliciano Dias Araújo Talita Antonia da Silveira Ricardo Alves de Olinda departamento de entomologia e acarologia Escola Superior de Agricultura “Luiz de Queiroz” Universidade de S&atilde o Paulo Piracicaba Brazil Centro de Ciências Agrárias Ambientais e Biológicas Universidade Federal do Rec&ocirc ncavo da Bahia Cruz das Almas Brazil departamento de estatística Centro de Ciência e Tecnologia Universidade Estadual da Paraíba Bodocongó Brazil

determining the levels of heavy metals in honey is a measure to control its quality. The objective of this study was to determine the contents of Cd, Cu, Pb and Zn in honey of stingless bee. The differential pulse anodic stripping voltammetry (DPASV) was used to determine the trace elements in honey samples. Pb was detected in 100% of the samples, Cu and Zn in 98.15%, and Cd only in 33.33%. The trace elements analyzed detected in the samples remained within tolerable levels in foods for human consumption.

关键词： Heavy Metals Stingless Bees Voltammetry Honey

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