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A novel secreted protein FgHrip1 from Fusarium graminearum triggers immune responses in plants

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Journal of Integrative Agriculture 2024年第11期 3774-3787页

作者： Zhenchao Fu Huiqian Zhuang Vincent Ninkuu Jianpei Yan Guangyue Li Xiufen Yang Hongmei Zeng State Key Laboratory for Biology of plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Fusarium graminearum, the primary pathogenic fungus responsible for Fusarium head blight(FHB) in wheat,secretes abundant chemical compounds that interact with host plants. In this study, a secreted protein FgHrip1,isolated from the culture filtrate of F. graminearum, was found to induce typical cell death in tobacco. The FgHrip1 gene was then cloned and expressed in Escherichia coli. Further bioassay analysis showed that the recombinant FgHrip1 induced early defense induction events, such as reactive oxygen species(ROS) production,callose deposition, and up-regulation of defense-related genes in tobacco. Furthermore, FgHrip1 significantly enhanced immunity in tobacco seedlings against Pseudomonas syringae pv. tabaci 6605(Pst. 6605) and tobacco mosaic virus(TMV). FgHrip1-treated wheat spikes also exhibited defense-related transcript accumulation and developed immunity against FHB infection. Whereas the expression of FgHrip1 was induced during the infection process, the deletion of the gene impaired the virulence of F. graminearum. Our results suggest that FgHrip1triggers immunity and induces disease resistance in tobacco and wheat, thereby providing new insight into strategy for biocontrol of FHB.

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Assessment of phytochemicals, antioxidant, anti-hemolytic, anti-inflammatory and anti-cancer potential of flowers, leaves and stem extracts of Rosa arvensis

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Journal of Traditional Chinese Medicine 2024年第4期44卷 804-812页

作者： Zubaria Tul Ain Iram Fatima Sana Naseer Sobia Kanwal Tariq Mahmood Department of plant Sciences Quaid-i-Azam UniversityIslamabad 45320Pakistan Department of Biology Allama Iqbal Open UniversityIslamabad 04403Pakistan Department of plant Sciences Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabad 45320Pakistan Pakistan Academy of Sciences Islamabad 04403Pakistan

OBJECTIVE:To evaluate phytochemicals and in vitro biological potential of flowers,leaves and stem extracts of Rosa ***:Presence of twenty secondary metabolites was confirmed and then phenolic and flavonoid contents were quantified *** Transform Infrared spectroscopy was conducted to ascertain functional groups and antioxidant potential was examined using 2,2-diphenyl-1-picrylhydrazyl scavenging activity,total antioxidant capacity and total reducing power *** erythrocytes were used to assess anti-hemolytic activity and five bacterial strains were examined to determine antibacterial potential of plant *** seeds were used to perform phytotoxic activity and cytotoxic potential was evaluated via brine shrimps and PC3 cell ***:Highest phenolic contents were detected in the methanolic extract of Rosa arvensis flower(RAFM)[(151.635±0.005)gallic acid equivalent mg/g]and highest flavonoid contents in the chloroform leaf extract(RALC)[(108.228±0.004)quercetin equivalent mg/g].Fourier-transform infrared spectroscopy analysis showed the presence of wide range of functional *** antioxidant assays indicated highest DPPH scavenging activity[IC50(23.5±0.6)μg/mL]in the methanolic stem extract(RASM),highest total antioxidant capacity[(265.1±0.9)μg/mL]in RAFM and highest reducing potential[(209.9±0.6)μg/mL]in leaf extract(RALM).Highest antihemolytic activity[(90.0±0.5)μg/mL]was recorded in RAFM and brine shrimp cytotoxicity potential[(52.3±0.3)μg/mL]in *** antimicrobial activity was detected highest[(21.1±0.5)mm inhibition zones]in RALM against Streptococcus *** the end,antiinflammatory and anti-cancer activity results depicted less than 50%inhibition in the methanolic ***:Our findings will be helpful in designing pharmaceutical regimens and therefore,more studies can be recommended to isolate and characterize compounds associated with the biological activities of Rosa arvensis.

关键词： anti-inflammatory agents spectroscopy,fourier transform infrared hemolysis oxidative stress PC-3 cells

Wood of trees:Cellular structure,molecular formation,and genetic engineering

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Journal of Integrative plant Biology 2024年第3期66卷 443-467页

作者： Yingying Zhu Laigeng Li State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems and College of Ecology Lanzhou UniversityLanzhou 730000China National Key Laboratory of plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China

Wood is an invaluable asset to human society due to its renewable nature,making it suitable for both sustainable energy production and material ***,wood derived from forest trees plays a crucial role in sequestering a significant portion of the carbon dioxide fixed during photosynthesis by terrestrial ***,with the expansion of the global population and ongoing industrialization,forest coverage has been substantially decreased,resulting in significant challenges for wood production and *** production practices have changed away from natural forests toward plantation ***,understanding the underlying genetic mechanisms of wood formation is the foundation for developing high-quality,fast-growing plantation *** ideal forest trees for wood production using genetic technologies has attracted the interest of *** studies have been carried out in recent years on the molecular,genetic,and cell-biological mechanisms of wood formation,and considerable progress and findings have been *** studies and findings indicate enormous possibilities and prospects for tree *** review will outline and assess the cellular and molecular mechanisms of wood formation,as well as studies on genetically improving forest trees,and address future development prospects.

关键词： cambium cell wall Populus wood formation xylem

The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

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The Crop Journal 2024年第4期12卷 1064-1080页

作者： Anna Nowicka Martin Kovacik Anna Maksylewicz Przemysław Kopec Ewa Dubas Monika Krzewska Agnieszka Springer Robert E.Hoffie Diaaeldin S.Daghma Zbynek Milec Ales Pecinka Jochen Kumlehn Iwona Zur The Franciszek Górski Institute of plant Physiology Polish Academy of Sciences Niezapominajek 2130-239 KrakówPoland Institute of Experimental Botany of the Czech Academy of Sciences Centre of Plant Structural and Functional GenomicsŠlechtitelu3177900 OlomoucCzech Republic Department of Cell Biology and Genetics Faculty of SciencePalacky UniversityŠlechtitelu°2777900 OlomoucCzech Republic Malopolska Centre of Biotechnology Jagiellonian University30-387 KrakówPoland Leibniz Institute of plant Genetics and Crop plant Research(IPK)Gatersleben Corrensstraße 306466 Stadt SeelandGermany Institute for Breeding Research on Horticultural Crops Julius Kühn Institute(JKI)-Federal Research Centre for Cultivated PlantsErwin-Baur Straße 2706484 QuedlinburgGermany Global Change Research Institute Czech Academy of Sciences Beˇlidla 986/4a60300 BrnoCzech Republic

plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental ***/doubled haploids(DHs)derived from in vitro-cultured microspores are widely used in plant breeding and genetic ***,the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly ***,RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis(ME)in barley cultivars Golden Promise and Igri,which exhibit contrasting responsiveness to microspore-derived plant *** experiments revealed fundamental regulatory networks,specific groups of genes,and transcription factor(TF)families potentially regulating the developmental *** identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to *** high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.

关键词： Hordeum vulgare Marker gene Microspore embryogenesis Stress response Transcriptome analysis

Silencing transformer and transformer-2 in Zeugodacus cucurbitae causes defective sex determination with inviability of most pseudomales

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Journal of Integrative Agriculture 2024年第3期23卷 938-947页

作者： Qin Ma Zizhen Fan Ping Wang Siya Ma Jian Wen Fengqin Cao Xianwu Lin Rihui Yan Key Laboratory of Green Prevention and Control of Tropical plant Diseases and Pests of Ministry of Education School of Plant ProtectionHainan UniversityHaikou 570228China School of Life Sciences HainanUniversityHaikou 570228China Hainan Yazhou Bay Seed Lab Sanya 572000China

transformer is a switch gene for sex determination in many insects, which cooperates with transformer-2 that is expressed in both sexes to regulate female differentiation, particularly in dipterans. Zeugodacus cucurbitae(Coquillett) is a very destructive pest worldwide, however, its sex determination pathway remains largely uncharacterized. Here, we show that the female sex ratio is sharply reduced with knockdown of either transformer or transformer-2 by RNA interference in early embryos of Z. cucurbitae. Most of the males grown from the embryos with transient transformer and transformer-2 suppression mated with wild-type females and produced mixed sex progeny, with one exception that produced only female progeny, and all of the few remaining males failed to mate with wild-type females and produced no progeny. The exceptional male and those males with mating failure were XX pseudomales as determined by the detection of Y chromosome-linked Maleness-on-the-Y, indicating that most XX pseudomales are not viable. The phenotypes of transformer and transformer-2 suggest that they play a key role in regulating sex determination and are required for female sexual development of Z. cucurbitae. Our results will be beneficial to the understanding of sex determination in Z. cucurbitae and can facilitate the development of genetic sexing strains for its biological control.

关键词： Zeugodacus cucurbitae transformer transformer-2 sex determination RNA interference biological control

FKF1b controls reproductive transition associated with adaptation to geographical distribution in maize

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Journal of Integrative plant Biology 2024年第5期66卷 943-955页

作者： Suhui Chen Shan Gao Dongyang Wang Jie Liu Yingying Ren Zhihan Wang Xin Wei Qin Wang Xuehui Huang Shanghai Key Laboratory of plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China

Maize(Zea mays subspecies mays)is an important commercial crop across the world,and its flowering time is closely related to grain yield,plant cycle and latitude ***1 is an essential clock-regulated blue-light receptor with distinct functions on flowering time in plants,and its function in maize remains *** this study,we identified two FKF1 homologs in the maize genome,named ZmFKF1a and ZmFKF1b,and indicated that ZmFKF1a and ZmFKF1b independently regulate reproductive transition through interacting with ZmCONZ1 and ZmGI1 to increase the transcription levels of ZmCONZ1 and *** demonstrated that ZmFKF1b underwent artificial selection during modern breeding in China probably due to its role in geographical ***,our data suggested that ZmFKF1bHap_C7 may be an elite allele,which increases the abundance of ZmCONZ1 mRNA more efficiently and adapt to a wider range of temperature zone than that of ZmFKF1bHap_Z58 to promote maize floral *** extends our understanding of the genetic diversity of maize *** allele is expected to be introduced into tropical maize germplasm to enrich breeding resources and may improve the adaptability of maize at different climate zones,especially at temperate region.

关键词： flowering geographical distribution maize ZmFKF1

SCAB1 coordinates sequential Ca^(2+) and ABA signals during osmotic stress induced stomatal closure in Arabidopsis

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Science China(Life Sciences) 2024年第1期67卷 1-18页

作者： Tianren Zhang Li Bai Yan Guo State Key Laboratory of plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Hyperosmotic stress caused by drought is a detrimental threat to plant growth and agricultural productivity due to limited water *** are gateways of transpiration and gas exchange,the swift adjustment of stomatal aperture has a strong influence on plant drought *** intensive investigations of stomatal closure during drought stress in past decades,little is known about how sequential signals are integrated during complete ***,we discovered that the rapid Ca^(2+) signaling and subsequent abscisic acid(ABA)signaling contribute to the kinetics of both F-actin reorganizations and stomatal closure in Arabidopsis thaliana,while STOMATAL CLOSURE-RELATED ACTIN BINDING PROTEIN1(SCAB1)is the molecular switch for this entire *** the early stage of osmotic shock responses,swift elevated calcium signaling promotes SCAB1 phosphorylation through calcium sensors CALCIUM DEPENDENT PROTEIN KINASE3(CPK3)and *** phosphorylation restrained the microfilament binding affinity of SCAB1,which bring about the Factin disassembly and stomatal closure *** the osmotic stress signal continued,both the kinase activity of CPK3 and the phosphorylation level of SCAB1 attenuated *** further found that ABA signaling is indispensable for these attenuations,which presumably contributed to the actin filament reassembly process as well as completion of stomatal ***,the dynamic changes of SCAB1 phosphorylation status are crucial for the kinetics of stomatal *** together,our results support a model in which SCAB1 works as a molecular switch,and directs the microfilament rearrangement through integrating the sequentially generated Ca^(2+) and ABA signals during osmotic stress induced stomatal closure.

关键词： calcium signal ABA stomatal movement microfilaments kinase

Gibberellin promotes cambium reestablishment during secondary vascular tissue regeneration after girdling in an auxin-dependent manner in Populus

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Journal of Integrative plant Biology 2024年第1期66卷 86-102页

作者： Yufei Zhang Lingyan Wang Yuexin Wu Donghui Wang Xin‐Qiang He State Key Laboratory of Protein and plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China

Secondary vascular tissue(SVT)development and regeneration are regulated by *** this study,we used an in vitro SVT regeneration system to demonstrate that gibberellin(GA)treatment significantly promotes auxin-induced cambium *** GA content by overexpressing or knocking down ent-kaurene synthase(KS)affected secondary growth and SVT regeneration in *** poplar DELLA gene GIBBERELLIC ACID INSENSITIVE(PtoGAI)is expressed in a specific pattern during secondary growth and cambium regeneration after *** of PtoGAI disrupted poplar growth and inhibited cambium regeneration,and the inhibition of cambium regeneration could be partially restored by GA *** analysis of the PtaDR5:GUS transgenic plants,the localization of PIN-FORMED 1(PIN1)and the expression of auxin-related genes found that an additional GA treatment could enhance the auxin response as well as the expression of PIN1,which mediates auxin transport during SVT *** together,these findings suggest that GA promotes cambium regeneration by stimulating auxin signal transduction.

关键词： auxin cambium gibberellin Populus secondary vascular tissue regeneration

A preliminary study on life history of marine red alga Stylonema alsidii(Zandardini)Drew *** Zheng et Li

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Journal of Oceanology and Limnology 2024年第3期42卷 915-922页

作者： Bingxin HUANG Yao ZHANG Yue CHU Youxuan GUO Junxia LIANG Jianan QIN Lanping DING Tianjin Key Laboratory of Animal and plant Resistance College of Life SciencesTianjin Normal UniversityTianjin 300387China

Stylonema alsidii(Zandardini)Drew *** Zheng et Li is a common species in the genus Stylonema,but there are limited reports on morphological taxonomy and a lack of studies on life history and the regulation of early *** early development,discoid formation,and erect thallus growth of Stylonema alsidii *** were observed under laboratory *** addition,the influence of main environmental factors such as temperature and light intensity on its development was briefly studied,and various stages of its life history were *** result shows:(1)there are two reproductive modes:asexual reproduction and sexual reproduction;(2)the morphology of this species varies in different degrees under laboratory culture conditions;(3)sexual reproduction and asexual reproduction can coexist under suitable *** pathways of life history were inferred from experimental *** sporophytes form mature gametophytes by producing initially isolated or initially attached monospore,and mature gametophytes form mature sporophytes by fertilization of eggs and *** sexual and asexual reproduction processes of Stylonema alsidii *** were reported for the first time,and this is also the first report on sexual reproduction in the genus Stylonema.

关键词： Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li life history environmental factor asexual reproduction sexual reproduction