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Electroacupuncture alleviates zymosan-induced colorectal hypersensitivity

Journal of Traditional Chinese Medicine 2025年第1期45卷 32-38页

作者： LI Siting WANG Shaojun YIN Yehui DE Gejing LI Caicai WANG Ziyan CAO Wenjie institute of Acupuncture and Moxibustion China Academy of Chinese Medical Sciences institute of Chinese materia medica China Academy of Chinese Medical Sciences

OBJECTIVE: this study to investigate the mechanism underlying the electroacupuncture(EA) alleviates colorectal hypersensitivity, a feature of irritable bowel syndrome(IBS). METHODS: The colorectal hypersensitivity model was established by treating mice with zymosan. Electrophysiological techniques, Western blotting and immunofluorescence staining were used to detect the changes of the sensitive state of the colorectum and the response in spinal ganglion and spinal cord after acupuncture intervention. RESULTS: colorectal distension studies revealed that repetitive applied electroacupuncture treatment on mice could significant alleviates colorectal intensity. Western blotting studies with nerve growth factor(NGF) in the colorectum, substance P(SP) in the spinal ganglion, protein kinase C gamma(PKCγ) in the spinal cord, and transient receptor potential vanilloid 1(TRPV1) showed that electroacupuncture suppressed zymosan-induced expression of TRPV1, NGF and SP in multiple tissues. Immunofluorescence labeling results showed that EA attenuated the expression of NGF in the colorectum, SP in the spinal ganglion, PKCγ in the spinal cord, and TRPV1 in all three tissues in zymosan-treated mice. Moreover, the number of neurons double-positive for TRPV1/Isolectin B4(IB4) and TRPV1/Neurofilament(NF) 200 was increased in the spinal ganglion. CONCLUSION: these results provide molecular-level evidence that EA alleviates zymosan-induced colorectal hypersensitivity by altering the expression of pain-associated proteins in the colorectum and spinal cord. EA has a potential to be therapeutic intervention option for IBS treatment.

关键词： colorectal hypersensitivity electroacupuncture nerve growth factor protein kinase C gamma substance P transient receptor potential channels

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Full biosynthetic pathway of pyrrolobenzoxazines

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Chinese Chemical Letters 2025年第1期36卷 433-436页

作者： Kunya Wang Bingyu Liu Daojiang Yan Jian Bai Haibo Yu Youcai Hu State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China

Pyrrolobenzoxazines are a rare terpene-amino acid family of natural products with potent biological ***,we reported the full biosynthetic pathway of paeciloxazine(1),a typical pyrrolobenzoxazine,with significant insecticidal *** on heterologous expression,chemical complement experiment,and in vitro biochemical assays,we demonstrated the sesquiterpene portion of 1 derived from discontinuously oxidations of amorphdiene,in which P450 monooxygenase PaxH catalyzed a cascade of hydroxylation and epoxidation,while two flavin dependent monooxygenases are involved in the transformation of the esterified tryptophan into a pyrrolobenzoxazine ***,a total of 15 compounds were generated through heterologous expression,of which 13,17 and 20 showed potential antiepileptic *** study fully elucidated the biosynthetic pathway of paeciloxazine(1)and showed the diversity and complexity of constructing natural products by organisms.

关键词： Pyrrolobenzoxazines Paeciloxazine Biosynthesis P450 monooxygenase Antiepileptic activity

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Three-step synthesis of flavanostilbenes with a 2-cyclohepten-1-one core by Cu-mediated [5+2] cycloaddition/decarboxylation cascade

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Chinese Chemical Letters 2025年第2期36卷 378-383页

作者： Gangsheng Li Xiang Yuan Fu Liu Zhihua Liu Xujie Wang Yuanyuan Liu Yanmin Chen Tingting Wang Yanan Yang Peicheng Zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College Beijing Wehand-Bio Pharmaceutical Co. Ltd.

The first synthesis of flavanostilbenes with a 2-cyclohepten-1-one core was carried out by applying an effective strategy in three steps from abundant polymerized flavanol resources. A key regio-and stereoselective Cu-mediated [5+2] cycloaddition/decarboxylation cascade was explored and applied without the use of protecting groups, and water as an environmentally friendly solvent contributed to the cascade. The intramolecular [5+2] cycloaddition mechanism, involving oxidation and dearomatization of the flavanol unit as a diene, was proposed and supported by the synthesis of the intermediate. The regioselectivity of the cyclization was found to be dependent on the substitution effects of the stilbene units by the exploration of substrate scope.

关键词： Biomimetic synthesis Copper Natural products Substrate [5 + 2] Cycloaddition

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Stem-leaf saponins of Panax notoginseng attenuate experimental Parkinson's disease progression in mice by inhibiting microglia-mediated neuroinflammation via P2Y2R/PI3K/AKT/NFκB signaling pathway

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Chinese Journal of Natural Medicines 2025年第1期23卷 43-53页

作者： Hui Wu Chenyang Ni Yu Zhang Yingying Song Longchan Liu Fei Huang Hailian Shi Zhengtao Wang Xiaojun Wu Shanghai Key Laboratory of Compound Chinese Medicines The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica The MOE Innovation Centre for Basic Medicine Research on Qi-Blood TCM Theories Shanghai University of Traditional Chinese Medicine

Stem-leaf saponins from Panax notoginseng(SLSP) comprise numerous PPD-type saponins with diverse pharmacological properties; however, their role in Parkinson's disease(PD),characterized by microglia-mediated neuroinflammation, remains unclear. This study evaluated the effects of SLSP on suppressing microglia-driven neuroinflammation in experimental PD models, including the 1-methyl-4-phenylpyridinium(MPTP)-induced mouse model and lipopolysaccharide(LPS)-stimulated BV-2 microglia. Our findings revealed that SLSP mitigated behavioral impairments and excessive microglial activation in models of PD, including MPTP-treated mice. Additionally, SLSP inhibited the upregulation of inducible nitric oxide synthase(i NOS) and cyclooxygenase-2(COX2) and attenuated the phosphorylation of PI3K,protein kinase B(AKT), nuclear factor-κB(NFκB), and inhibitor of NFκB protein α(IκBα) both in vivo and in vitro. Moreover, SLSP suppressed the production of inflammatory markers such as interleukin(IL)-1β, IL-6, and tumor necrosis factor alpha(TNF-α) in LPS-stimulated BV-2cells. Notably, the P2Y2R agonist partially reversed the inhibitory effects of SLSP in LPStreated BV-2 cells. These results suggest that SLSP inhibit microglia-mediated neuroinflammation in experimental PD models, likely through the P2Y2R/PI3K/AKT/NFκB signaling pathway. These novel findings indicate that SLSP may offer therapeutic potential for PD by attenuating microglia-mediated neuroinflammation.

关键词： Microglia NFκB Neuroinflammation P2Y2 receptor Stem-leaf saponins of Panax Notoginseng

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Assembly and network of Rhei Radix et Rhizoma surface microbiome shaped by processing methods and sampling locations

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Chinese Herbal Medicines 2025年第1期17卷 189-199页

作者： Guangfei Wei Xiao Chen Guozhuang Zhang Conglian Liang Zhaoyu Zhang Bo Zhang Shilin Chen Linlin Dong State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences School of Biomedicine Beijing City University Shandong University of Traditional Chinese Medicine School of Pharmacy Linyi University institute of Herbgenomics Chengdu University of Traditional Chinese Medicine

Objective:Rhei Radix et Rhizoma has five types of products,namely,raw rhubarb(RR),wine rhubarb(WR),vinegar rhubarb(VR),cooked rhubarb(CR),and rhubarb charcoal(RC).However,Rhei Radix et Rhizoma is easily contaminated with fungi and mycotoxins if not harvested or processed ***,we intend to analyze how microbiome assemblies and co-occurrence patterns are influenced by sampling locations and processing ***:High-throughput sequencing and internal transcribed spacer 2(ITS2) were carried out to study the diversities(α-and β-diversity),composition(dominant taxa and potential biomarkers),and network complexitity of surface fungi on RR, WR,VR,CR,and RC collected from Gansu and Sichuan provinces,***:The phyla Ascomycota and Basidiomycota;the genera Kazachstania,Malassezia,and Asterotremella;and the species Kazachstania exigua,Asterotremella pseudolonga,and Malassezia restricta were the dominant fungi and exhibited differences in the two provinces and the five processed *** α-diversity and network complexity were strongly dependent on processing *** 1,the Shannon index,and network complexity and connectivity were highest in the CR *** α-diversity and network complexity were influenced by sampling *** 1 and network complexity and connectivity were highest in the Gansu ***:The assembly and network of the surface microbiome on Rhei Radix et Rhizoma were shaped by processing methods and sampling *** paper offers a comprehensive understanding of microorganisms,which can provide early warning for potential mycotoxins and ensure the safety of drugs and consumers.

关键词： community assembly fungi processing methods Rhei Radix et Rhizoma sampling locations

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Shenlian Extract Protects against Ultrafine Particulate Matter-Aggravated Myocardial Ischemic Injury by Inhibiting Inflammation and Cell Apoptosis

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Biomedical and Environmental Sciences 2025年第2期38卷 206-218页

作者： Shuiqing Qu Yan Liang Shuoqiu Deng Yu Li Yue Dai Chengcheng Liu Tuo Liu Luqi Wang Lina Chen Yujie Li institute of Chinese materia medica China Academy of Chinese Medical SciencesBeijing 100700China Artemisinin Research Center China Academy of Chinese Medical SciencesBeijing 100700China

Objective Emerging evidence suggests that exposure to ultrafine particulate matter(UPM,aerodynamic diameter<0.1μm)is associated with adverse cardiovascular *** studies have found that Shenlian(SL)extract possesses anti-inflammatory and antiapoptotic properties and has a promising protective effect at all stages of the atherosclerotic disease *** this study,we aimed to investigated whether SL improves UPM-aggravated myocardial ischemic injury by inhibiting inflammation and cell *** We established a mouse model of MI+*** measurement,measurement of myocardialinfarct size,biochemical analysis,enzyme-linked immunosorbent assay(ELISA),histopathological analysis,Transferase dUTP Nick End Labeling(TUNEL),Western blotting(WB),Polymerase Chain Reaction(PCR)and so on were used to explore the anti-inflammatory and antiapoptotic effects of SL in vivo and in *** SL treatment can attenuate UPM-induced cardiac dysfunction by improving left ventricular ejection fraction,fractional shortening,and decreasing cardiac infarction *** significantly reduced the levels of myocardial enzymes and attenuated UPM-induced morphological ***,SL significantly reduced expression levels of the inflammatory cytokines IL-6,TNF-α,and *** further increased the infiltration of macrophages in myocardial tissue,whereas SL intervention reversed this *** also triggered myocardial apoptosis,which was markedly attenuated by SL *** results of in vitro experiments revealed that SL prevented cell damage caused by exposure to UPM combined with hypoxia by reducing the expression of the inflammatory factor NF-κB and inhibiting apoptosis in H9c2 *** Overall,both in vivo and in vitro experiments demonstrated that SL attenuated UPMaggravated myocardial ischemic injury by inhibiting inflammation and cell *** mechanisms were related to the downregulation of macrophages infiltrating heart tiss

关键词： Ultrafine particulate matter Shenlian extract Inflammation Apoptosis Macrophage

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Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening

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Science China(Life Sciences) 2025年第1期68卷 300-302页

作者： Ze-Min Lin Mai Xiang Wen-Hui Wei Shu-Hui Fan Li Chen Jie Wang Xiao-Qian Yang Chun-Hao Yang Shi-Jun He Jian-Ping Zuo Laboratory of Immunopharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China School of Pharmacy University of Chinese Academy of SciencesBeijing 100049China School of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China State Key Laboratory of Drug Research Department of Medicinal ChemistryShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Innovation Research institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese MedicineShanghai 201203China Experiment Center for Science and Technology Shanghai University of Traditional Chinese MedicineShanghai 201203China

Dear Editor,Phenotypic drug discovery,which observes compound effects on physiology,has given way to targeting specific molecules,but has revived by using modern methods to find drugs based on therapeutic outcomes in disease models(Vincent et al.,2022).Osteoarthritis(OA)is a prevalent chronic joint disorder,primarily affecting knee joints,with osteoclast activation due to low-grade inflammation resulting in increased bone resorption and structural changes in the joint(Katz et al.,2021).This imbalance and the discharge of inflammatory mediators perpetuate the cycle of inflammation and tissue ***,controlling inflammation and modulating osteoclastogenesis are critical therapeutic targets for managing OA.

关键词： inflammation drugs

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Atractylenolide Ⅰ ameliorates post-infectious irritable bowel syndrome by inhibiting the polymerase Ⅰ and transcript release factor and c-Jun N-terminal kinase/inducible nitric oxide synthase pathway

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Journal of Traditional Chinese Medicine 2025年第1期45卷 57-65页

作者： YUAN Jianan CHENG kunming LI Chao ZHANG Xiang DING Zeyu LI Bing ZHENG Yongqiu Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui Teaching and Research Section of Traditional Chinese MedicineSchool of PharmacyWannan Medical College institute of Chinese materia medica China Academy of Chinese Medical Sciences

OBJECTIVE: To explore the therapeutic effect and target of atractylenolide I(AT-I) on post-infectious irritable bowel syndrome(PI-IBS) rats. METHODS: Therefore, the preliminarily mechanism of AT-I in anti-PI-IBS were first predicted by network pharmacology and molecular docking, then the possible signaling pathways were systematically analyzed. Finally, the potential therapeutic targets and possible signaling pathways of AT-I on PI-IBS in Sprague-Dawley(SD) rat model were verified by experiments. RESULTS: AT-I could alleviate PI-IBS symptoms and reduce the expression of tumor necrosis factor α, interleukin-6 and Interferon-gamma in PI-IBS SD rat model and inhibit the c-Jun N-terminal kinase/inducible nitric oxide synthase(JNK/iNOS) pathway. Notably, AT-I treatment could inhibit the overexpression of polymerase Ⅰ and transcript release factor(PTRF). CONCLUSION: AT-I could alleviate PI-IBS symptoms through downregulation of PTRF and inhibiting the JNK/iNOS pathway. This study not only provides a scientific basis to clarify the anti-PI-IBS effect of AT-I and its mechanism but also suggests a novel promising therapeutic strategy to treat the PI-IBS.

关键词： MAP kinase signaling system atractylenolide Ⅰ network pharmacology nitric oxide synthase type Ⅱ polymerase Ⅰ and transcript release factor post-infectious irritable bowel syndrome

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TPMGD:A genomic database for the traditional medicines in Pakistan

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Chinese Herbal Medicines 2025年第1期17卷 87-93页

作者： Rushuang Xiang Huihua Wan Wei Sun Baozhong Duan Weiqian Chen Xue Cao Sifan Wang Chi Song Shilin Chen Yan Wang Atia-tul Wahab M.Iqbal Choudhary Xiangxiao Meng State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences College of Pharmaceutical Science Dali University H.E.J.Research institute of Chemistry International Center for Chemical and Biological Sciences University of Karachi Dr.Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological Sciences University of Karachi

Objective:In Pakistan,traditional medicines are an important component of the medical system,with numerous varieties and great ***,due to the scattered resources and the lack of systematic collection and collation,adulteration of traditional Pakistani medicine(TPM) is common,which severely affects the safety of their medicinal use and the import and export ***,it is urgent to systematically organize and unify the management of TPM and establish a set of standards and operable methods for the identification of ***:We collected and organized the information on 128 TPMs with regard to their medicinal parts,efficacy,usage,and genetic material,based on Pakistan Hamdard Pharmacopoeia of Eastern Medicine:Pharmaceutical *** genetic information of TPM is summarized from national center for biotechnology information(NCBI) and global pharmacopoeia genome database(GPGD).Furthermore,we utilized bioinformatics technology to supplement the chloroplast genome(cp-genome) data of 12 *** build the web server,we used the Linux+Apache+MySQL+PHP(LAMP) system and constructed the webpage on a PHP:Hypertext Preprocessor(PHP) model view controller(MVC) ***:We constructed a new genomic database,the traditional Pakistani medicine genomic database(TPMGD).This database comprises five entries,namely homepage,medicinal species,species identification,basic local alignment search tool(BLAST),and ***,TPMGD contains basic profiles of 128 TPMs and genetic information of 102 TPMs,including 140 cytochrome c oxidase subunit I(COI)sequences and 119 mitochondrial genome sequences from Bombyx mori,1 396 internal transcribed spacer 2(ITS2) sequences and 1 074 intergenic region(psbA-trnH) sequences specific to 92 and 83 plant species,***,TPMGD includes 199 cp-genome sequences of 82 ***:TPMGD is a multifunctional database that integrates species description,functional information inquiry,genetic information

关键词： chloroplast genome database DNA barcodes Pakistan traditional medicine

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Metabolome and transcriptome association study reveals biosynthesis of specialized benzylisoquinoline alkaloids in Phellodendron amurense

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Chinese Herbal Medicines 2025年第1期17卷 178-188页

作者： Tingxia Liu Wanran Zhang Sijia Wang Ya Tian Yifan Wang Ranran Gao Shilin Chen Wei Sun Wei Ma Zhichao Xu College of Pharmaceutical Sciences Heilongjiang University of Chinese Medicine Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences College of Life Sciences Northeast Forestry University

Objective:Benzylisoquinoline alkaloids(BIAs) have pharmacological functions and clinical *** are mainly distributed in plant species across the order Ranunculales and the genus Phellodendron from *** BIA biosynthesis has been intensively investigated in Ranunculales ***,the accumulation mechanism of BIAs in Phellodendron is largely *** aim of this study is to unravel the biosynthetic pathways of BIAs in Phellodendron ***:The transcriptome and metabolome data from 18 different tissues of *** were meticulously sequenced and subsequently subjected to a thorough *** gene co-expression network analysis(WGCNA),a powerful systems biology approach that facilitates the construction and subsequent analysis of co-expression networks,was utilized to identify candidate genes involved in BIAs *** this,recombinant plasmids containing candidate genes were expressed in Escherichia coli,a widely used prokaryotic expression *** purpose of this genetic engineering endeavor was to express the candidate genes within the bacteria,thereby enabling the assessment of the resultant enzyme ***:The synonymous substitutions per synonymous site for paralogs indicated that at least one whole genome duplication event has *** potential BIA biosynthetic pathway of *** was proposed,and two PR10/Bet v1 members,14 CYP450s,and 33 methyltransferases were selected as related to BIA *** PR10/Bet v1 was identified as norcoclaurine synthase,which could catalyze dopamine and 4-hydroxyphenylacetaldehyde into(S)-***:Our studies provide important insights into the biosynthesis and evolution of BIAs in nonRanunculales species.

关键词： benzylisoquinoline alkaloids biosynthesis evolution norcoclaurine synthase Phellodendron amurense Rupr

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