检索结果-南通市图书馆

Highly efficient and automated extraction of DNA from human remains using a modified EZ1 protocol

Forensic Sciences Research 2021年第1期6卷 59-66页

作者： Anna Barbaro Sasha Samar Giacomo Falcone Angelo La Marca Department of Forensic Genetics Studio Indagini Mediche E Forensi(SIMEF)Reggio CalabriaItaly

Bones and teeth often represent the only sources of DNA available for identifying human *** in bones and teeth is generally better preserved than that in soft tissues because of the presence of hard connective tissue with a high level of *** of the extensive mineralisation,the choice of an efficient DNA extraction procedure is important to minimise the sampling of a high level of minerals and to remove polymerase chain reaction(PCR)*** protocols are available for DNA extraction from bones and teeth as part of the Qiagen EZ1 DNA Investigator Kit using the EZ1 Advanced XL automated purification *** improve the efficiency of DNA extraction from skeletal remains,the present study focuses on a modification to these already available *** this study,different bones and teeth collected between 1 and 50 years after death were subjected to DNA extraction using the standard EZ1 protocol,a supplementary protocol,and a modified *** modified approach included a decalcification step,whereas the Qiagen protocols worked directly on non-decalcified *** all three procedures,150 mg samples were used for DNA *** evaluated the quantity of DNA recovered from samples,the presence of any PCR inhibitors co-extracted,the level of DNA degradation,the quality of short tandem repeat(STR)profiles,and the reproducibility of the modified *** compared with the other protocols,the modified protocol resulted in the best recovery of DNA that was free of PCR ***,the STR profiles were reliable and of high *** our opinion,the decalcification step increases DNA recovery by softening tissues,which allows lysis solutions to act more ***,the use of two lysis solutions and the variation added to the EZ1 purification step allow for DNA recovery with quality and quantity superior to those of the previously available Qiagen-based *** findings may be helpful solutions to t

关键词： Forensic sciences forensic genetics DNA extraction bone tooth EZ1 automation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fertigation with Wastewater and Vermicompost: Soil Biochemical and Agronomic Implications

引用

Pedosphere 2014年第5期24卷 625-634页

作者： G.MASCIANDARO E.PERUZZI S.DONI C.MACCI Consiglio Nazionale delle Ricerche (CNR) Istituto per lo Studio degli Ecosistemi (ISE) Via Moruzzi 1 Pisa 56124 (Italy)

To study how wastewater(WW) and different organic sources(humic substances or vermicompost) affected soil chemical and biochemical fertility and agronomic productivity in field-grown melon, an experiment arranged in a randomized complete block design with three replications was conducted with the following treatments: three different mixtures of irrigation water(100% WW, 50% WW with 50% groundwater, and 100% groundwater) and, for each WW treatment, four different organic fertilization treatments of control without organic matter application(CK), vermicompost used as surface mulch(SM), vermicompost incorporated into the soil(VC),and humic substances extracted by vermicompost(HS). Chemical and biochemical results indicated an improvement in soil fertility,suggested by the increase in soil organic carbon and nutrient(nitrate and macro- and microelements) contents and in soil microbial activities(hydrolytic and oxidative enzymes), in particular in the VC treatments. In addition, even soil potential metabolism was stimulated by WW combined with organic treatments, as highlighted by the increase in the metabolic(dehydrogenase activity/watersoluble carbon) and nitrification indices(NO-3and NH+4). Melon productivity confirmed these results, with the highest yield and melon quality in the VC treatments irrigated with 100% WW. In conclusion, the combined use of WW and organic amendment,recovering both mineral and organic nutrients from these kinds of recycled materials, was effective in the improvement of soil quality and crop productivity.

关键词： Cucumis melo enzyme activity humic substance organic matter soil quality

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Optimization Study about Fault Self-Healing Restoration of Power Distribution Network Based on Multi-Agent Technology

引用

Computers, Materials & Continua 2020年第10期65卷 865-878页

作者： Fuquan Huang Zijun Liu Tinghuang Wang Haitai Zhang Tony Yip Shenzhen Power Utility Co. Ltd.Shenzhen518000China Shandong Kehui Electric Power Automation Co. Ltd.Zibo255000China Kehui International Ltd. Studio 206Mill Studio Business CentreCrane MeadWareHertsSG129PYUK

In order to quickly and accurately locate the fault location of the distribution network and increase the stability of the distribution network,a fault recovery method based on multi-objective optimization algorithm is *** optimization of the power distribution network fault system based on multiagent technology realizes fast recovery of multi-objective fault,solve the problem of network learning and parameter adjustment in the later stage of particle swarm optimization algorithm falling into the local extreme value dilemma,and realize the multi-dimensional nonlinear optimization of the main grid and the auxiliary *** system proposed in this study takes power distribution network as the goal,applies fuzzy probability algorithm,simplifies the calculation process,avoids local extreme value,and finally realizes the energy balance between each power *** results show that the Multi-Agent Technology enjoys priority in restoring important load,shortening the recovery time of power grid balance,and reducing the overall line loss rate of power ***,the power grid fault self-healing system can improve the safety and stability of the important power grid,and reduce the economic loss rate of the whole power grid.

关键词： Multi agent technology power distribution network fault self-healing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

May be Player

引用

优品 2014年第5期 40-47页

作者：曾嵘黎晓亮 Alexvi studio

这场时装的夏日游戏谁是谁手中的Queen?绿色褶皱间探出一段婀娜柳腰足底踩一出水晶平跟用色彩反复洗牌Get it。

关键词：游戏私人物品褶皱时装绿色色彩足底白色夏日水晶

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

想“泰”多 Thinkk studio的泰式设计

引用

艺术与设计 2018年第4期7卷 80-87页

作者：甄建恒 Thinkk studio

从曼谷设计节和清迈设计周，到今年年初首次登场的曼谷设计周，看得出来，泰国也充斥着源源不绝的设计人才，其襄盛举地展现着设计本领。但这当中又有多少能突破重围，获得国际性的喝彩呢？答案是真的不多。而这当中能叫得出名的当属Thi... 详细信息

从曼谷设计节和清迈设计周，到今年年初首次登场的曼谷设计周，看得出来，泰国也充斥着源源不绝的设计人才，其襄盛举地展现着设计本领。但这当中又有多少能突破重围，获得国际性的喝彩呢？答案是真的不多。而这当中能叫得出名的当属Thinkk studio工作室。

关键词：设计人才国际性工作室曼谷

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

THINKK studio:功能的趣味表达

引用

设计 2012年第11期25卷 132-137页

作者： yxiang THIHKK studio

泰国设计师们一直在积极努力，为世界设计地图增添一抹东南亚风采，他们吸收西方设计思维，融合自身文化血统，打造出具有丰富文化层次的日常用品，来自泰国的设计师DECHA ARCHJANANUN和PLOYPAN THEERACHAI虽年纪轻轻，却将自己的建筑... 详细信息

泰国设计师们一直在积极努力，为世界设计地图增添一抹东南亚风采，他们吸收西方设计思维，融合自身文化血统，打造出具有丰富文化层次的日常用品，来自泰国的设计师DECHA ARCHJANANUN和PLOYPAN THEERACHAI虽年纪轻轻，却将自己的建筑专业背景与产品设计融合得恰到好处。

关键词：趣味功能文化层次设计师设计思维日常用品产品设计专业背景

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Aeromonas veronii biovar veronii and sepsis-infrequent complication of biliary drainage placement: A case report

引用

World Journal of Clinical Cases 2019年第6期7卷 759-764页

作者： Manlio Monti Arianna Torri Elena Amadori Alice Rossi Giulia Bartolini Chiara Casadei Giovanni Luca Frassineti Department of Medical Oncology Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS Unit of Microbiology Wide Catchment Area of Romagna Laboratory Radiology Unit Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

BACKGROUND Aeromonas species are uncommon pathogens in biliary sepsis and cause substantial mortality in patients with impaired hepatobiliary function. Asia has the highest incidence of infection from Aeromonas,whereas cases in the west are *** SUMMARY We report the case of a 64-year-old woman with advanced pancreatic cancer and jaundice who manifested fever,abdominal pain,severe thrombocytopenia,anemia and kidney failure following the insertion of a percutaneous transhepatic biliary drainage. Blood culture results revealed the presence of Aeromonas veronii biovar veronii(A. veronii biovar veronii). After antibiotic therapy and transfusions,the life-threatening clinical conditions of the patient improved and she was *** This was a rare case of infection,probably the first to be reported in West countries,caused by A. veronii biovar veronii following biliary drainage. A finding of Aeromonas must alert clinician to the possibility of severe sepsis.

关键词： Aeromonas veronii biovar veronii Aeromonas Pancreatic cancer Cholangitis Sepsis Biliary tract infection Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Complete pathological response in locally advanced non-small-cell lung cancer patient: A case report

引用

World Journal of Clinical Cases 2021年第20期9卷 5540-5546页

作者： Elisabetta Parisi Donatella Arpa Giuglia Ghigi Simona Micheletti Elisa Neri Luca Tontini Martina Pieri Antonino Romeo Radiotherapy Unit IRCCS Istituto Scientifico Romagnolo per lo Studio dei Tumori(IRST)"Dino Amadori"Meldola 47014Italy

BACKGROUND Chemotherapy and radiotherapy followed by durvalumab is currently the standard treatment for locally advanced node-positive non-small-cell lung cancer(NSCLC).We describe the case of a patient with locally advanced node-positive NSCLC(LA-NSCLC)treated in a phase II prospective protocol with chemotherapy,accelerated hypofractionated radiotherapy(AHRT)and surgery in the preimmunotherapy *** SUMMARY A 69-year-old male,ex-smoker(20 PY),with a Karnofsky performance status of 90,was diagnosed with locally advanced squamous cell lung *** was staged by total body computed tomography(CT)scanning,and integrated 18Ffluorodeoxyglucose positron emission tomography/CT scan[cT4 cN3 cM0,stage IIIC according to TNM(tumor-node-metastasis)8th edition]and received AHRT between chemotherapy cycles,in accordance with the study protocol(EudractCT registration 2008-006525-14).At the end of the study the patient underwent surgery,which was not part of the protocol,and showed a complete pathological *** This case report confirms that AHRT can be used successfully to treat primary LA-NSCLC with bilateral mediastinal lymph node *** case is of particular interest because of the pathological response after AHRT and the lack of surgical *** hypothesize that this radiotherapeutic approach,with its proven efficacy,could be delivered as a short course reducing treatment costs,increasing patient compliance and reducing *** are currently investigating the possibility of combining hypofractionation,chemotherapy and immunotherapy for patients with LA-NSCLC.

关键词： Locally advanced non-small-cell lung cancer Hypofractionated radiotherapy Chemoradiotherapy Complete pathological response Immunotherapy Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

以纯粹的设计,探索空间可能性——CJ studio创始人陆希杰演讲实录

引用

设计家 2017年第2期 50-53页

作者：许晓东陆希杰 CJ studio

＂建筑、设计不只是单纯的艺术创造,更是社会实践,是一个看世界的入口。＂CJ studio创始人陆希杰,攻读东海大学建筑系和英国AA建筑联盟硕士期间,深受中西方文化、传统与现代、国际化与地域性等文化或思潮碰撞的影响。学生时代对建筑建... 详细信息

＂建筑、设计不只是单纯的艺术创造,更是社会实践,是一个看世界的入口。＂CJ studio创始人陆希杰,攻读东海大学建筑系和英国AA建筑联盟硕士期间,深受中西方文化、传统与现代、国际化与地域性等文化或思潮碰撞的影响。学生时代对建筑建构理念的讨论、研究,在真正从事建筑、室内设计之后,转化为对空间设计要义的思考与探索,无论是几何的折叠、展开、分类、漂移,还是灌铸、造型、数位.

关键词：室内设计空间设计创始人中西方文化实录建筑系艺术创造社会实践

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

伍凯宏:AimSound studio联合创始人蛮荒大地上的音乐行者

引用

中国商人 2011年第12期 112-113页

作者：马原 AimSound studio

一切从音乐开始中国商人：您是从什么时候开始走上了音乐道路的？

关键词：音乐行者大地中国商人

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：