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Diagnostische Bedeutung von pentavalenter ^(99m)Tc-Dimercaptobern-steinsure ([V]-DMSA) bei Kopf-Hals-Tumoren

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2000年第4期20卷 303-307页

作者：安锐 Abteilung für nuklearmedizin Xiehe Hospital Tongji Medizinische Universität Wuhan 430022 China klinik und poliklinik für nuklearmedizin Universität Bonn Bonn 53127 Germany

The purpose of this investigation was to assess the biodistribution of 99mTc-[V]-DMSA in human being, and its di- agnostic value in patients with head and neck tumours. 20 patients with histologically confirmed head and neck tumours were ex- amined with planar as well as SPECT scintigraphy. Whole body scintigraphy in different time after injection of 99mTc-[V]-DM- SA was performed to assess the major sequential organ biodistribution. Our results showed that the blood clearance of 99mTc- [V]-DMSA was bi-exponential. All organs except kidneys showed a relatively rapid elimination of 99mTc-[V]-DMSA. The kid- neys showed a increasing accumulation in the first 2 h, which is probably due to the tubular reabsorption of 99mTc-[V]-DMSA. In 15 of 20 patients, 19 lesions could be proven by means of planar scintigraphy (corresponding sensitivity of 75%). 29 lesions in 18 patients could however be detected by the application of SPECT (corresponding sensitivity of 90%). Except primary tumours and local lymphadenmetastases in 5 patients distant metastases (3 thorax wall, 1 liver and 1 inguinal/paravesicle) were found. Altogether a sensitivity of 76. 9% and a specificity of 71. 4% were calculated for the detection of primary tumour. The sensitivi- ty and specificity for the exploration of lymphadenmetastases were 75% and 100%. In conclusion, this study shows that 99m Tc-[V]-DMSA, particularly with SPECT imaging, is useful in localising the primary tumours and lymphadenmetastases as well as distant metastases of head and neck tumours. The possible therapeutic application of 188/186re-[V]-DMSA is also evaluated.

关键词： mTc-[V]-DMSA head and neck tumour: diagnosis

Dietary copper triggers onset of fulminant hepatitis in the Long-Evans cinnamon rat model

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World Journal of Gastroenterology 2012年第39期18卷 5542-5550页

作者： ramsi Siaj Vanessa Sauer Sandra Stppeler Hans-Ullrich Spiegel Gabriele Khler Andree Zibert Hartmut HJ Schmidt klinik und poliklinik für Transplantationsmedizin Universittsklinikum MünsterD-48149 MünsterGermany Abteilung Chirurgische forschung Klinik und Poliklinik für Allgemein-und ViszeralchirurgieUniversittsklinikum MünsterD-48149 MünsterGermany Gerhard-Domagk-Institut für Pathologie Universittsklinikum MünsterD-48149 MünsterGermany

AIM： To investigate the impact of dietary copper given at different time points on the onset of fulminant hepatitis. METHODS： The Long-Evans cinnamon （LEC） rat mod- el of Wilson＇s disease （WD） was used to study the im- pact of high dietary copper （hCu） on the induction of fulminant hepatitis at early or late time points of life. High Cu diet was started in rat pups or in adults （month 5） for three months. Animals that received reduced di- etary copper （rCu） throughout their lifetime served as a control. Hepatitis-associated serum markers （alanine aminotransferase, aspartate transaminase, bilirubin） were analyzed in animal groups receiving hCu or rCu. Liver copper content and liver histology were revealed at sacrifice. A set of 5 marker genes previously found to be affected in injured liver and which are related to angiogenesis （Vegfa）, fat metabolism （Srebf1）, ex- tracellular matrix （Timp1）, oxidative stress （Hmox1）, and the cell cycle （Cdknla） were analyzed by real-time polymerase chain reaction. rESULTS： regardless of the time point when hCu was started, LEC rats （35/36） developed fulminant hepati- tis and died. Animals receiving rCu （36/36） remained healthy, did not develop hepatitis, and survived long term without symptoms of overt disease, although liver copper accumulated in adult animals （477 ± 75 μg/g）. With regard to start of hCu, onset of fulminant hepatitis was significantly （P 〈 0.001） earlier in adults （35±9 d） that showed pre-accumulation of liver copper as com- pared to the pup group （77±15 d）. Hepatitis-associ- ated serum markers, liver copper and liver histology, as well as gene expression, were affected in LEC rats re- ceiving hCu. However, except for early and rapid onset of hepatitis, biochemical and molecular markers were similar at the early and late time points of disease. CONCLUSION： rapid onset of fulminant hepatitis in asymptomatic LEC rats with elevated liver copper sug- gests that there is a critical threshold of liver copper which is imp

关键词： Wilson's disease fulminant hepatitis Acuteliver failure Dietary copper Long-Evans cinnamon rat A TP7B

Open label trial of granulocyte apheresis suggests therapeutic efficacy in chronically active steroid refractory ulcerative colitis

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World Journal of Gastroenterology 2005年第44期11卷 7001-7006页

作者： Wolfgang Kruis Axel Dignass Elisabeth Steinhagen-Thiessen Julia Morgenstern Joachim M(o|¨)ssner Stephan Schreiber Maurizio Vecchi Alberto Malesci Max reinshagen robert L(o|¨)fberg Evangelisches Krankenhaus Kalk Medizinische klinik für Hepatologie und Gastroenterologie Medizinische klinik und poliklinik Ⅱ Medizinische klinik Dep of Int Medicine IrCCS Ospedale Policlinico Istituto Clinico Humanitas Innere Medizin Karolinska Institute at the IBD unit

AIM： To study the efficacy, safety, and feasibility of a granulocyte adsorptive type apheresis system for the treatment of patients with chronically active ulcerative colitis despite standard therapy. METHODS： An open label multicenter study was carried out in 39 patients with active ulcerative colitis （CAI 6-8） despite continuous use of steroids （a minimum total dose of 400 mg prednisone within the last 4 wk）. Patients received a total of five aphereses using a granulocyte adsorptive technique （Adacolumn, Otsuka Pharmaceutical Europe, UK）. Assessments at wk 6 and during follow-up until 4 mo comprised clinical （CAI） and endoscopic （EI） activity index, histology, quality of life （IBDQ）, and laboratory tests. rESULTS： Thirty-five out of thirty-nine patients were qualified for intent-to-treat analysis. After the apheresis treatment at wk 6, 13/35 （37.1%） patients achieved clinical remission and 10/35 （28.6%） patients had endoscopic remission （CAI〈4, EI〈4）. Quality of life （IBDQ） increased significantly （24 points, P〈0.01） at wk 6. Apheresis could be performed in all but one patient. Aphereses were well tolerated, only one patient experienced anemia. CONCLUSION： In patients with steroid refractory ulcerative colitis, five aphereses with a granulocyte/ monocyte depleting filter show potential short-term efficacy. Tolerability and technical feasibility of the procedure are excellent.

关键词： Steroid refractory colitis Ulcerative colitis Granulocyte Apheresis

Positive predictors for gastroesophageal reflux disease and the therapeutic response to proton-pump inhibitors

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World Journal of Gastroenterology 2014年第14期20卷 4017-4024页

作者： Valentin Becker Stefan Grotz Christoph Schlag Simon Nennstiel Analena Beitz Bernhard Haller roland M Schmid Alexander Meining Monther Bajbouj Ⅱ. Medizinische klinik und poliklinik Technische Universit?t München Institut für Medizinische Statistik und Epidemiologie Technische Universit?t München

AIM: To identify objective and subjective predictors for the reliable diagnosis of gastroesophageal reflux disease (GErD) and the response to proton pump inhibitor (PPI) therapy.

关键词： Gastroesophageal reflux Gastroesophageal reflux disease Non-erosive reflux disease Impedance pH measurement follow-up Therapy Proton pump inhibitor

Epigastric hernias in children and the use of ultrasound in its diagnosis

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World Journal of Pediatric Surgery 2023年第2期6卷 92-96页

作者： Tatjana T König Laura S Oerters Ljuba Spiller Martin Schwind Mark Born Christina Oetzmann von Sochaczewski Andreas C Heydweiller klinik und poliklinik für Kinderchirurgie Universitätsmedizin der Johannes Gutenberg-Universität MainzMainzGermany Sektion Kinderchirurgie der klinik und poliklinik für Allgemein- Viszeral-Thorax-und GefächirurgieUniversität sklinikum BonnBonnGermany Sektion Kinderradiologie der klinik für Diagnostische und Interventionelle radiologie Universitätsklinikum BonnBonnGermany

Introduction There is a paucity of clinical data on pediatric epigastric hernias despite them accounting for up to 6%of all hernia repairs in *** aimed to provide additional data to supplement those 117 cases of a recent systematic review and to further clarify the role of ultrasound in diagnosing pediatric epigastric *** We retrospectively included all 60 patients treated for epigastric hernias in children in two tertiary pediatric surgical departments within 12 *** were tested via point-biserial correlation *** Epigastric hernias primarily affected preschool children with a median age of 39 *** vast majority of patients(88%)presented with swelling that was occasionally(30%)accompanied by *** defects could be found during clinical examination in 45%of patients with a median size of 5 mm(95%CI 3 to 10).Smaller defects were less likely to be palpable(r=-0.44,95%CI-0.08 to-0.7,p=0.021).Likewise,ultrasound was used more frequently with smaller fascial defect sizes(r=-0.51,95%Cl-0.16 to-0.74,p=0.007).Laparoscopic repair was used in 11 patients(19%)and more often(4/11)in combination with another simultaneous procedure than open repair(11/48).Conclusions Epigastric hernias are primarily a condition of the preschool *** can be beneficial if the diagnosis cannot be made clinically;otherwise,it is abdicable if it does not change the management of the patient's epigastric *** repairs might be beneficial for children with multiple defects or simultaneous procedures.

关键词： diagnosis primarily otherwise

reduced expression of Ca^(2+)-regulating proteins in the upper gastrointestinal tract of patients with achalasia

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World Journal of Gastroenterology 2006年第37期12卷 6002-6007页

作者： Harald fischer Judith fischer Peter Boknik Ulrich Gergs Wilhelm Schmitz Wolfram Domschke Jan W Konturek Joachim Neumann Medizinische klinik und poliklinik B Universittsklinikum Mnster 48129 Mnster Germany Institut fr Pharmakologie und Toxikologie Universittsklinikum Mnster 48149 Mnster Germany Institut fr Pharmakologie und Toxikologie Martin-Luther-Universitt Halle-Wittenberg 06112 Halle (Saale) Germany klinik fr Innere Medizin/Gastroenterologie Elbe Klinikum Stade 21682 Stade Germany

AIM: To compare expression of Ca2+-regulating proteins in upper gastrointestinal (GI) tract of achalasia patients and healthy volunteers and to elucidate their role in achalasia. METHODS: Sarcoplasmic reticulum Ca2+ ATPase (SErCA) isoforms 2a and 2b, phospholamban (PLB), calsequestrin (CSQ), and calreticulin (CrT) were assessed by quantitative Western blotting in esophagus and heart of rats, rabbits, and humans. furthermore, expression profi les of these proteins in biopsies of lower esophageal sphincter and esophagus from patients with achalasia and healthy volunteers were analyzed. rESULTS: SErCA 2a protein expression was much higher in human heart (cardiac ventricle) compared to esophagus. However, SErCA 2b was expressed predominantly in the esophagus. The highest CrT expression was noted in the human esophagus, while PLB, although highly expressed in the heart, was below our detection limit in upper GI tissue. Compared to healthy controls, CSQ and CrT expression in lower esophageal sphincter and distal esophageal body were signif icantly reduced in patients with achalasia (P f lesser importance for regulation of SErCA than in heart. Lower expression of Ca2+ storage proteins (CSQ and CrT) might contribute to increased lower esophageal sphincter pressure in achalasia, possibly by increasing free intracellular Ca2+.

关键词： Esophageal and gastric motility Esophagus Calsequestrin Calreticulin

Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

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World Journal of Gastroenterology 2005年第14期11卷 2080-2087页

作者： Marcus K.H. Auth Kim A. Boost Kerstin Leckel Wolf-Dietrich Beecken Tobias Engl Dietger Jonas Elsie Oppermann Philip Hilgard Bernd H. Markus Wolf-Otto Bechstein roman A. Blaheta Universit(a|¨)ts-Kinderklinik Essen Abteilung für Allgemeine P(a|¨)diatrie Germany Zentrum der An(a|¨)sthesiologie und Wiederbelebung Johann Wolfgang Goethe-Universit(a|¨)t Frankfurt am MainGermany klinik für Allgemein-und Gef(a|¨)βchirurgie Germany klinik für Urologie und Kinderurologie Zentrum der Chirurgie Johann Wolfgang Goethe-Universit(a|¨)tFrankfurt am MainGermany klinik für Allgemein- und Gef(a|¨)βchirurgie Germany Universit(a|¨)tsklinikum Essen Abteilung für Gastroenterologie und Hepatologie Germany klinik für Allgemein- und Viszeralchirurgie St(a|¨)dt. klinikum Kemperhof KoblenzGermany

AIM:Clinical application of human hepatocytes (HC) is hampered by the progressive loss of growth and differentiation in vitro. The object of the study was to evaluate the effect of a biphasic culture technique on expression and activation of growth factor receptors and differentiation of human adult HC. METHODS: Isolated HC were sequentially cultured in a hormone enriched differentiation medium (DM) containing nicotinamide, insulin, transferrin, selenium, and dexame-thasone or activation medium (AM) containing hepatocyte growth factor (HGf), epidermal growth factor (EGf), and granulocyte-macrophage colony-stimulating factor (GMCSf). Expression, distribution and activation of the HC receptors (MET and EGfr) and the pattern of characteristic cytokeratin (CK) filaments were measured by fluorometry, confocal microscopy and Western blotting. rESULTS: In the biphasic culture system, HC underwent repeated cycles of activation (characterized by expression and activation of growth factor receptors) and re-differentiation (illustrated by distribution of typical filaments CK-18 but low or absent expression of CK-19). In AM increased expression of MET and EGfr was associated with receptor translocation into the cytoplasm and induction of atypical CK-19. In DM low expression of MET and EGfr was localized on the cell membrane and CK-19 was reduced. receptor phosphorylation required embedding of HC in collagen type I gel. CONCLUSION: Control and reversible modulation of growth factor receptor activation of mature human HC can be accomplished in vitro, when defined signals from the extracellular matrix and sequential growth stimuli are provided. The biphasic technique helps overcome dedifferentiation, which occurs during continuous stimulation by means of growth factors.

关键词： Human hepatocytes Differentiation Hepatocyte growth factor Epidermal growth factor HGf receptor EGf receptor Cytokeratin Collagen gel

Treatment of eosinophlic esophagitis with swallowed topical corticosteroids

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World Journal of Gastroenterology 2020年第36期26卷 5395-5407页

作者： Simon Nennstiel Christoph Schlag klinikum rechts der Isar der Technischen Universität München Klinik und Poliklinik für Innere Medizin IIMunich 81675Germany

Eosinophilic esophagitis(EoE)is an emerging chronic local immune-mediated disease of the *** proton pump inhibitors and food-restrictiondiets swallowed topical corticosteroids(STC)can be offered as a first line therapy according to current *** review describes the background and practical management of STCs in *** far,mainly asthma inhalers containing either budesonide or fluticasone have been administered to the esophagus by swallowing these medications“off label”.recently esophagus-targeted formulations of topical steroids have been developed showing clinicopathological response rates up to 85%-an orodispersible tablet of budesonide has been approved as the first“in label”medication for EoE in Europe in June *** it was shown that disease remission induction of EoE by STCs is highly effective,there is still a lack of data regarding long-term and maintenance ***,current studies on STC maintenance therapy add some movement into the game.

关键词： Eosinophilic esophagitis Swallowed topical corticosteroids Esophagustargeted formulations of topical steroids fluticasone Budesonide Budesonide orodispersible tablet Budesonide oral suspension

Targeting metabolic rewiring might decrease spread of tumor cells: Mitochondrial trNA modifications promote cancer metastasis

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Signal Transduction and Targeted Therapy 2022年第11期7卷 3987-3988页

作者： Maik Luu Alexander Visekruna Lehrstuhl für Zelluläre Immuntherapie Medizinische Klinik und Poliklinik IIUniversitätsklinikum WürzburgWürzburgGermany Institute for Medical Microbiology and Hygiene Philipps-UniversityMarburgGermany

In a recent study published in Nature,Delaunay and colleagues demonstrate that trNA modifications in mitochondria have the potential to affect the synthesis of mitochondrial proteins involved in oxidative phosphorylation(OXPHOS),which drives the invasive spread of cancer *** inhibition of one particular rNA-modifying enzyme was sufficient to prevent the cancer cell invasion and dissemination.

关键词： invasion metastasis cancer