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Dynamic phase transition behavior of a LiMn_(0.5)Fe_(0.5)PO_(4) olivine cathode material for lithium-ion batteries revealed through in-situ X-ray techniques

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Journal of Energy Chemistry 2022年第8期31卷 452-459,I0012页

作者： Sujeera Pleuksachat Phongsit Krabao Sarawut Pongha Viyada Harnchana Pawinee Klangtakai Wanwisa Limphirat Siriwat Soontaranon Jeffrey Nash Nonglak Meethong Department of Physics Faculty of ScienceKhon Kaen UniversityKhon Kaen 40002Thailand Institute of Nanomaterials Research and Innovation for Energy(IN-RIE) Research Network of NANOTEC-KKU(RNN)Khon Kaen UniversityKhon Kaen 40002Thailand Synchrotron Light Research Institute(SLRI) Nakhon Ratchasima 30000Thailand

LiMn_(0.5)Fe_(0.5)PO_(4) has attracted great interest due to its good electrochemical performance and higher operating *** has led to a greater than 30 percent higher energy density than for commercial Li Fe PO4 olivine *** the phase transition behaviors and kinetics of this material will help researchers to design and develop next generation cathodes for Li-ion *** this study,we investigated non-equilibrium phase transition behaviors in a LiMn_(0.5)Fe_(0.5)PO_(4) cathode material during charge–discharge processes by varying current rates(C-rates)using synchrotron in-situ X-ray *** methods included wide angle X-ray scattering(in-situ WAXS)and X-ray absorption spectroscopy(in-situ XAS).The WAXS spectra indicate that the phase transition of LiMn_(0.5)Fe_(0.5)PO_(4) material at slow C-rates is induced by a two-phase *** contrast,at a high C-rate(5 C),the formation of an intermediate phase upon discharge is clearly ***,the oxidation numbers of the redox reactions of Fe^(2+)/Fe^(3+)and Mn^(2+)/Mn^(3+)were evaluated using in-situ *** combination of synchrotron in-situ X-ray techniques gives clear insights into the non-equilibrium phase transition behavior of a LiMn_(0.5)Fe_(0.5)PO_(4) cathode *** new understanding will be useful for further developments of this highly promising cathode material for practical commercialization.

关键词： LiMn_(0.5)Fe_(0.5)PO_(4) Phase transition In-situ X-ray techniques

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Can immediate postoperative radiographs predict outcomes in pediatric clubfoot?

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World Journal of Orthopedics 2022年第11期13卷 986-992页

作者： Duangjai Leeprakobboon Department of Orthopedic Khon Kaen HospitalMuang 40000Khon KaenThailand

BACKGROUND The goal of treatment for pediatric idiopathic clubfoot is to enable the patient to comfortably walk on his or her soles without ***,currently accepted treatment protocols are not always *** on the abnormal bone alignment reported in this disease,some studies have noted a correlation between radiographic characteristics and outcome,but this correlation remains *** To assess the correlation between immediately postoperative radiographic parameters and functional outcomes and to identify which best predicts functional *** To predict the outcome and prevent early failure of the Ponseti’s method,we used a simple radiographic method to predict *** study included newborns with idiopathic clubfoot treated with Ponseti’s protocol from November 2018 to August *** Achilles tenotomy and a long leg cast were applied,the surgeon obtained a single lateral *** parameters included the tibiocalcaneal angle(TiCal),talocalcaneal angle(TaCal),talofirst metatarsal angle(Ta1st)and tibiotalar angle(TiTa).During the follow-up period,the Dimeglio score and functional score were examined 1 year after ***,recurring events were *** correlation between functional score and radiographic characteristics was analyzed using sample and multiple logistic regression,and the optimal predictor was also *** In total,54 feet received approximately 8 manipulations of casting and Achilles tenotomy at a mean age of 149 *** average TiCal,TaCal,Ta1st,and TiTa angles were 75.24,28.96,7.61,and 107.31 degrees,*** 12 mo of follow up,we found 66%excellent-to-good and 33.3%fair-to-poor functional *** Dimeglio score significantly worsened in the poor outcome group(P value<0.001).Tical and TaCal showed significant differences between each functional outcome(P value<0.05),and the TiCal strongly correlated with outcome,with a smaller angle indicating a better outco

关键词： Idiopathic clubfoot Radiograph Functional outcome Tenotomy Prognostic Tibiocalcaneal angle

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MUC1 and MUC5AC mucin expression in liver fluke-associated intrahepatic cholangiocarcinoma

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World Journal of Gastroenterology 2005年第32期11卷 4939-4946页

作者： Chanchai Boonla Banchob Sripa Peti Thuwajit Ubon Cha-On Anucha Puapairoj Masanao Miwa Sopit Wongkham Department of BiochemistryLiver Fluke and Cholangiocarcinoma Research Center Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of PathologyLiver Fluke and Cholangiocarcinoma Research Center Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of PathologyLiver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen UniversityKhon Kaen 40002Thailand Institute of Basic Medical SciencesUniversity of Tskuba TsukubaIbaraki 305-8575Japan

AIM： To investigate the expressions of MUC1 and MUC5AC in intrahepatic cholangiocarcinoma （ICC）. Association of expressions of mucins MUC1 and MUC5AC with clinical findings, metastasis, and survival of the liver fluke-associated ICC patients was determined. METHODS： The expressions of MUC1 and MUC5AC mucins were examined by immunohistochemical staining in 87 cases of histologically-proven ICC. The expressions of mucins in relationship between clinicopathological significance and prognosis of the patients were evaluated. RESULTS： Fifty-two patients （60%） exhibited both MUC1 and MUCSAC expressions, whereas 31% expressed either MUC1 or MUC5AC, and 9% expressed neither. High MUC1 immunoreactivity displayed a significant correlation wibh tumor progression as reflected by vascular invasion （P〈0.001）, whereas high expression of MUC5AC significantly correlated with neural invasion （P = 0.022） and advanced ICC stage （P = 0.008）. Patients with high expression of MUC1 had a significantly shorter survival （P = 0.0002）. According to multivariate analyses, MUC1 reactivity （P = 0.026）, histological grading and stage of tumor represented the least probability of survival. CONCLUSION： MUC1 is overexpressed in liver flukeassociated cholangiocarcinoma and relates to vascular invasion and poor prognosis, whereas MUC5AC mucin is neoexpressed and relates to neural invasion and advanced ICC stage. High MUC1 expression in tumor may be useful for predicting the poor outcome of ICC patients.

关键词： Cholangiocarcinoma Mucin MUC1 MUC5AC Invasion Survival Immunohistochemistry

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Amplification of chromosome 21q22.3 harboring trefoil factor family genes in liver fluke related cholangiocarcinoma is associated with poor prognosis

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World Journal of Gastroenterology 2006年第26期12卷 4143-4148页

作者： Kanuengnuch Muenphon Temduang Limpaiboon Patcharee Jearanaikoon Chawalit Pairojkul Banchob Sripa Vajarabhongsa Bhudhisawasdi Department of Clinical Chemistry Center for Research and Development of Medical Diagnostic Laboratories Faculty of Associated Medical SciencesKhon Kaen UniversityKhon Kaen 40002Thailand Department of Pathology Liver Fluke and Cholangiocarcinoma Research Center Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of Surgery Liver Fluke and Cholangiocarcinoma Research Center Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand

AIM： To determine allelic imbalance on chromosomal region 21q22-qter including trefoil factor family genes （TFF） in cholangiocarcinoma （CCA） patients and analyze the correlation between allelic imbalances and clinicopathological parameters. METHODS： Quantitative PCR amplification was performed on four microsatellite markers and trefoil factor family genes （TFF1, TFF2, and TFF3） using a standard curve and SYBR Green I dye method. The relative copy number was determined by DNA copy number of tested locus to reference locus. The relative copy number was interpreted as deletion or amplification by comparison with normal reference range. Associations between allelic imbalance and clinicopathological parameters of CCA patients were evaluated by χ^2-tests. Kaplan-Meier method was used to analyze survival. RESULTS： The frequencies of amplification at D21S1890, D21S1893, and TFF3 were 32.5%, 30.0%, and 28.7%, respectively. Patients who had amplification at regions covering D21S1893, D21S1890, and TFF showed poor prognosis, whereas patients who had deletion showed favorable prognosis （mean： 51.7 wk vs 124.82 wk, P = 0.012）. Multivariate Cox regression analysis revealed that amplification of D21S1893, D21S1890 and TFF, blood vessel invasion, and staging were associated with poor prognosis. CONCLUSION： D21S1893-D21S1890 region may harbor candidate genes especially TFF and serine protease family, which might be involved in tumor invasion and metastasis contributing to poor survival. The amplification in this region may be used as a prognostic marker in the treatment of CCA patients.

关键词： Cholangiocarcinoma Amplification on chromosome 21 Trefoil factor family Quantitative PCR Liver fluke

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Selection of proper combine harvesters to field conditions by an effective field capacity prediction model

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International Journal of Agricultural and Biological Engineering 2020年第4期13卷 125-134,I0013页

作者： Khunnithi Doungpueng Khwantri Saengprachatanarug Jetsada Posom Somchai Chuan-Udom Department of Agricultural Engineering Faculty of EngineeringKhon Kaen UniversityKhon Kaen 40002Thailand Applied Engineering for Important Crops of the North East Research Group Khon Kaen UniversityKhon Kaen 40002Thailand

Farmers have to finish their harvesting with high efficiency,because of time and ***,farmers are lacking knowledge and information required for selecting suitable combine harvesters and giving the conditions of their rice fields,because both information factors(combine harvester and field condition)impact the field *** field capacity model was generated from combine harvesters with the Thai Hom Mali rice variety(KDML-105).Therefore,this study aimed to determine the prediction model for effective field capacity to combine harvesters when harvesting the Thai Hom Mali rice variety(KDML-105).The methods began by collecting data of 15 combine harvesters,such as field,crop,and machine conditions and operating times;to generate the prediction model for the KDML-105 *** prediction model was then validated using 12 combine harvesters that were collected similarly to the model *** results showed a root mean square error(RMSE)of 0.24 m^(2)/s for the *** prediction model can be applied for farmers to select the proper combine harvesters and give their field conditions.

关键词： rice harvesting combine harvester prediction model effective field capacity selection of combine harvester

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Inflammatory cytokines suppress arylamine N-acetyltransferase 1 in cholangiocarcinoma cells

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World Journal of Gastroenterology 2007年第46期13卷 6219-6225页

作者： Benjaporn Buranrat Auemduan Prawan Banchob Sripa Veerapol Kukongviriyapan Department of Pharmacology Faculty of MedicineLiver Fluke and Cholangiocarcinoma Research CenterKhon Kaen UniversityKhon Kaen 40002Thailand Department of Pathology Faculty of MedicineLiver Fluke and Cholangiocarcinoma Research CenterKhon Kaen UniversityKhon Kaen 40002Thailand

AIM： To evaluate the effect of inflammatory cytokines on arylamine N-acetyltransferase 1 （NAT1）, which is a phase-U enzyme involved in the biotransformation of aromatic and heterocyclic amines found in food, drugs and the environment. METHODS： Human cholangiocarcinoma KKU-100 cells were treated with a mixture of proinflammatory cytokines （interferon-7, interleukin-l and tumor necrosis factor-m） for 48 h, and the effect on NAT1 activity was assessed by high performance liquid chromatography, while NAT1 expression was determined by reverse-transcription polymerase chain reaction. The oxidative stress on the cells was examined by the formation of nitric oxide, superoxide anion and glutathione （GSH） levels. The cells were also treated with S-nitroso-glutathione （GSNO）, a nitric oxide donor, to see if the responses were similar to those obtained with the inflammatory cytokines. RESULTS： Cytokines suppressed NAT1 activity, reducing the Vmax without affecting the Am. Cytokines also had a significant impact on the induction of nitric oxide production and in reducing the redox ratios of glutathione （GSH） and GSH disulfide. Treatment with GSNO for 2-48 h reduced NAT1 activity without affecting the GSH ratio. Moreover, inflammatory cytokines and GSNO suppressed NAT1 mRNA expression. CONCLUSION： These findings indicate an association between inflammation and suppression of NAT1, which perhaps contributes to chemical-mediated toxicity and carcinogenesis,

关键词： Arylamine N-acetyltransferase 1 Phase lldrug-metabolizing enzyme Inflammatory cytokine Oxidative stress Cholangiocarcinoma

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Peanut testa extracts enhance anticancer effect of cisplatin against human cholangiocarcinoma cells via modulation of histone deacetylase inhibitory activity

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Asian Pacific Journal of Tropical Biomedicine 2020年第8期10卷 369-378页

作者： Somprasong Saenglee Gulsiri Senawong Jarckrit Jeeunngoi Sanun Jogloy Albert JKetterman Banchob Sripa Thanaset Senawong Department of Biochemistry Faculty of ScienceKhon Kaen UniversityKhon Kaen 40002Thailand Department of Plant Science and Agricultural Resources Faculty of AgricultureKhon Kaen UniversityKhon Kaen 40002Thailand Institute of Molecular Biosciences Mahidol UniversitySalaya Campus73170 Thailand Department of Pathology Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Natural Product Research Unit Faculty of ScienceKhon Kaen UniversityKhon Kaen 40002Thailand

Objective:To investigate the effect of combination treatments of cisplatin and KK4 and ICG15042 peanut testa extracts against cholangiocarcinoma cells in ***:The growth inhibition,cell cycle arrest and apoptosis of cholangiocarcinoma cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry analysis,*** levels of proteins involved in apoptosis were assessed using Western blotting *** caspase activity was assessed using a colorimetric caspase activity ***:Cisplatin and peanut(KK4 and ICG15042)testa extracts inhibited the growth of cholangiocarcinoma cell lines(KKUM214 and KKU-100 cells)in a dose-and time-dependent *** combination treatments reduced cell viability and induced apoptosis of cholangiocarcinoma cells more efficiently than singledrug *** cell death synergistically mediated by cisplatin and peanut testa extracts was observed in KKU-M214 cells(combination index1.0).The combination treatments also increased the subG1 population and caused KKU-M214 cell cycle arrest at S and G2/M phases,which were the combined effects of cisplatin(S phase arrest)and peanut testa extracts(G2/M phase arrest).In addition,p ERK1/2,Ac-H3,Bcl-2 and proteins related to apoptosis,including Bax and caspases 3,8,9,exhibited enhanced expression in KKUM214 *** combination treatments caused down-regulation of p53,whereas the expression of p21 was fairly constant when compared with cisplatin single drug ***:Peanut testa extracts in combination with cisplatin synergistically reduce cell viability and induce apoptosis through stimulation of caspases 3,8 and 9 in KKU-M214 cells.

关键词： Apoptosis Caspases Cholangiocarcinoma Cisplatin Natural histone deacetylase inhibitor Peanut testa extracts

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Prognostic significance of microsatellite alterations at 1p36 in cholangiocarcinoma

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World Journal of Gastroenterology 2006年第27期12卷 4377-4382页

作者： Temduang Limpaiboon Sumonta Tapdara Patcharee Jearanaikoon Banchob Sripa Vajarabhongsa Bhudhisawasdi Department of Clinical Chemistry Center for Research and Development of Medical Diagnostic LaboratoriesFaculty of Associated Medical SciencesKhon Kaen UniversityKhon Kaen 40002Thailand Department of Pathology Liver Fluke and Cholangiocarcinoma Research CenterFaculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of Surgery Liver Fluke and Cholangiocarcinoma Research CenterFaculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand

AIM： To investigate loss of heterozygosity （LOH） and microsatellite instability （MSI） on the chromosomal region 1p36-pter in cholangiocarcinoma （CCA） patients and determine the association between microsatellite alterations and clinicopathological parameters. METHODS： Ten polymorphic microsatellite markers were determined for LOH and MSI using GS-3000 gel scan fragment autoanalyzer. RESULTS： Sixty-eight out of 90 cases （75.6%） showed LOH in one or more loci. LOH was found most frequently at DIS199 （40.0%）, DIS507 （34.6%）, DIS2845 （30.5%）, and DIS2734 （30.1%）. MSI was found in 34 of 90 cases （37.8%） at one or more loci. Fine mapping at lp36 showed two distinctive regions of common loss, which were D1S2845 and the 25.5-cM region between D1S507 and D1S2734, indicating the existence of putative tumor suppressor genes that is likely to play important roles in the development of CCA. Patients with LOH at D1S234 showed less lymphatic invasion （P = 0.017）, whereas patients with LOH at D1S2676 exhibited more lymphatic invasion than those without （P = 0.031）. LOH at D1S2845 showed a significant correlation with nerve invasion （P = 0.029）. Moreover, patients who demonstrated MSI at D1S228 showed a poor prognosis （P = 0.0026）. CONCLUSION： Allelic loss plays a major role in microsatellite alterations at chromosome lp36, which may contribute to carcinogenesis and pathogenesis of liver fluke related CCA and these alterations can be used as molecular prognostic indicators for CCA patients.

关键词： Cholangiocarcinoma Liver fluke Chromosome 1p36 Loss of heterozygosity Microsatellite instability

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Compliance with enhanced recovery after surgery predicts long-term outcome after hepatectomy for cholangiocarcinoma

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World Journal of Gastrointestinal Surgery 2023年第3期15卷 362-373页

作者： Chaowasaporn Jongkatkorn Vor Luvira Chalisa Suwanprinya Kantaruthai Piampatipan Natwutpong Leeratanakachorn Theerawee Tipwaratorn Attapol Titapun Tharatip Srisuk Suapa Theeragul Apiwat Jarearnrat Vasin Thanasukarn Ake Pugkhem Narong Khuntikeo Chawalit Pairojkul Supot Kamsa-Ard Vajarabhongsa Bhudhisawasdi Department of Surgery Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of Surgery Saraburi HospitalSaraburi 18000Thailand Department of Pathology Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of Epidemiology and Biostatistics Faculty of Public HealthKhon Kaen UniversityKhon Kaen 40002Thailand

BACKGROUND Enhanced recovery after surgery(ERAS)program has been proved to improve postoperative outcome for many surgical procedures,including liver *** was limited evidence regarding the feasibility and benefit of ERAS in patients who underwent liver resection for *** To evaluate the feasibility of ERAS in patients who underwent liver resection for cholangiocarcinoma and its association with patient *** We retrospectively analyzed 116 cholangiocarcinoma patients who underwent hepatectomy at Srinagarind hospital,khon kaen University between January 2015 and December *** primary outcome was the compliance with *** determine the association between ERAS compliance and patient *** patients were categorized into those adhering more than and equal to 50%(ERAS≥50),and below 50%(ERAShospital stay was significantly shorter in the ERAS≥50 group[8.9 d,95%confidence interval(CI):7.3-10.4 d]than in the ERAShospital mortality occurred in the ERAS≥50 *** survival was significantly higher in the ERAS≥50 *** median survival of the patients in the ERAShospital,short-term,an

关键词： Enhanced recovery program after surgery Cholangiocarcinoma Hepatectomy Survival Enhanced recovery after surgery Outcome

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Inhibition of two stages of melanin synthesis by sesamol,sesamin and sesamolin

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Asian Pacific Journal of Tropical Biomedicine 2017年第10期7卷 886-895页

作者： Montra Srisayam Natthida Weerapreeyakul Kwanjai Kanokmedhakul Faculty of Science and Technology Pibulsongkram Rajabhat UniversityPhitsanulok65000Thailand Faculty of Pharmaceutical Sciences Khon Kaen UniversityKhon Kaen40002Thailand Faculty of Sciences Khon Kaen UniversityKhon Kaen40002Thailand

Objective: To investigate the antimelanogenesis properties of three sesame compoundssesamol, sesamin and sesamolin via two stages of melanin synthesis vis-à-vis sunscreen function and enzyme inhibition in melanoma cell line in order to search for alternative depigmenting ***: Antimelanogenic effects of sesame lignans were assessed in SK-MEL2 compared with the reference depigmenting agents, kojic acid and b-arbutin, in order to evaluate:(a) the sunscreen function of sesamol, sesamin and sesamolin by measurement of UV absorbtion property;(b) the inhibition of tyrosinase activity through mushroom and cellular tyrosinase; and(c) the effect on melanin content and melanogenic protein expression(tyrosinase, TRP-1 and TRP-2) by Western blot analysis; and(d) the toxicity of sesamol, sesamin and sesamolin to cells using cell cytotoxicity ***: The results showed that sesamin, sesamolin and sesamol exerted satisfiable sunscreen function by absorbed UVB at 290 nm. Sesamol exhibited the highest inhibition of mushroom tyrosinase activity, but lipophilic sesamolin exhibited the highest cellular tyrosinase inhibition(IC_(50) of 1.6 μM) followed by sesamin, sesamol, and kojic acid,respectively. The order from high to low inhibition of melanin pigment was detected in the SK-MEL2 treated with sesamolin, sesamin, sesamol, kojic acid, and b-arbutin,respectively. Sesamolin and sesamin successfully inhibited cellular tyrosinase activity and respectively decreased TRP-1/TRP-2(36%/15%) and TRP-1 levels(16%), thereby inhibiting melanogenesis via antityrosinase activity. No cytotoxicity to SK-MEL2 or Vero(normal) cell lines was observed at the lignan concentrations that exerted an antimelanogenic ***: Three sesame lignans prevent melanin synthesis through 2 stages:(a) by blocking melanin-induction and(b) by interrupting melanogenic enzyme production. This study provides evidence that sesamol, sesamin and sesamolin are potential for antimelanogenesis agents.

关键词： Sesamol Sesamin Sesamolin Tyrosinase TRP-1/TRP-2 Antimelanogenesis

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