检索结果-南通市图书馆

WheatGmap:a comprehensive platform for wheat gene mapping and genomic studies

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2021年第2期14卷 187-190页

作者： Lichao Zhang Chunhao Dong Zhongxu Chen Lixuan Gui Cheng Chen Danping Li Zhencheng Xie Qiang Zhang Xueying Zhang Chuan Xia Xu Liu Xiuying Kong Jirui Wang key laboratory for crop gene resources and germplasm enhancement Ministry of Agriculture and Rural Affairs/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130China State key laboratory of crop gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China Department of Life Science TcuniInc.Chengdu 610041China

Bulked segregant analysis(BSA)is an efficient and low-cost strategy that is widely used to identify causal genes in segregating ***-based methods,such as BSA sequencing(Wenger et al.,2010),bulked segregant RNA sequencing(BSR-seq)(del Viso et al.,2012),and MutMap(Abe et al.,2012),are powerful tools that can be used for rapidly discovering genetic markers and gene *** BSA is increasingly being used in wheat(Triticum aestivum)gene mapping efforts,few user-friendly BSA tools have been developed for researchers lacking a strong bioinformatics ***,we developed the web-based BSA platform WheatGmap(https://***),which integrates multiple BSA mapping models and large amounts of public data to accelerate gene cloning and functional research and facilitate resource sharing.

关键词： aestivum Wheat comprehensive

Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2023年第3期65卷 755-771页

作者： Hang Yang Yunlong Wang Yunlu Tian Xuan Teng Zehui Lv Jie Lei Erchao Duan Hui Dong Xue Yang Yuanyan Zhang Yinglun Sun Xiaoli Chen Xiuhao Bao Rongbo Chen Chuanwei Gu Yipeng Zhang Xiaokang Jiang Wenyu Ma Pengcheng Zhang Yi Ji Yu Zhang Yihua Wang Jianmin Wan State key laboratory for crop genetics and germplasm enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National key Facility for crop resources and genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Most of the reported P-type pentatricopeptide repeat(PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22(flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR *** of FLO22 resulting in defective transsplicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function. RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly upregulated. In addition to involvement in splicing as a P-type PPR protein, we found that FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing. The present work indicated that FLO22 plays an important role in endosperm development and plant growth by participating in nad1 maturation and multi-site editing of mitochondrial messager RNA.

关键词： endosperm development Oryza sativa PPR proteins RNA editing RNA splicing

BRITTLE CULM16(BRITTLE NODE) is required for the formation of secondary cell walls in rice nodes

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2017年第6期16卷 1286-1293页

作者： WANG Ying REN Yu-long CHEN Sai-hua XU Yang ZHOU Kun-neng ZHANG Long MING Ming WU Fu-qing LIN Qi-bing WANG Jiu-lin GUO Xiu-ping ZHANG Xin LEI Cai-lin CHENG Zhi-jun WAN Jian-min National key Facility for crop gene resources and genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China National key laboratory for crop genetics and germplasm enhancement Nanjing Agricultural University Nanjing 210095 P. R. China

Plant cell walls constitute the skeletal structures of plant bodies,and thus confer lodging resistance for grain *** the basic cell wall synthesis machinery is relatively well established now,our understanding of how the process is regulated remains limited and *** this study,we report the identification and characterization of the novel rice（Oryza sativa L.）brittle culm16（brittle node;bc16）*** brittle node phenotype of the bc16 mutant appears exclusively at nodes,and resembles the previously reported bc5 *** histochemical staining and electron microscopy assays revealed that in the bc16 mutant,the secondary cell wall formation and thickening of node sclerenchyma tissues are seriously affected after ***,cell wall composition assays revealed that the bc16 mutation led to a significant reduction in cellulose and lignin *** a map-based cloning approach,the bc16 locus is mapped to an approximately 1.7-Mb region of chromosome ***,our findings strengthen evidence for discretely spatial differences in the secondary cell wall formation within plant bodies.

关键词： rice (Oryza sativa L.) brittle node sclerenchyma tissue secondary cell wall

Cloning and Expression of gene Responsible for High-Tillering Dwarf Phenotype in Indica Rice Mutant gsor23

在线全文

学校读者我要写书评

暂无评论

Rice science 2013年第5期20卷 320-328页

作者： YUAN Shou-jiang WANG Tao YIN Liang ZHAO Jin-feng WAN Jian-min LI Xue-yong National key Facility for crop gene resources and genetic Improvement Institute of Crop ScienceChinese Academy of Agriculture Sciences Shandong Rice Research Institute National key laboratory for crop genetics and germplasm enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural University

High-tillering dwarf mutant gsor23 was generated from an indica rice variety Indica9 radiatied by y-ray. genetic analysis showed that this phenotype was controlled by one single recessive gene, which was mapped within a physical distance of 386 kb between two insertion-deletion （InDel） markers CI-WT2 and C1-WT4 on the long arm of chromosome 1. There is a known gene DIO within this region, the mutation of which causes high-tillering in rice. Sequence analysis of the DIO allele in gsor23 revealed that the base cytosine （C） at the 404th position in the coding region was deleted, which would cause frameshift mutation after the 134th amino acids. The mutation site and indica background of gsor23 were different from the previously reported japonica mutants d10-1 and d10-2. Therefore, gsor23 is a novel allelic mutant of D10 which encodes the carotenoid-cleaving dioxygenase 8 （CCD8）, a key enzyme involved in the biosynthesis of the new plant hormone strigolactones （SLs）. After treatment with GR24, a synthetic analogue of SLs, the high-tillering phenotype of gsor23 was restored to normal. Real-time RT-PCR analysis showed that D10 expression was high in roots, but low in leaves. Compared with the wild type Indica9, the expression of the SL biosynthesis gene DIO was upregulated, while genes likely involved in the SL signal transduction pathway such as D3 and D14 were down-regulated in the gsor23 mutant.

关键词： high-tillering dwarf mutant strigolactone carotenoid-cleaving dioxygenase feedback regulation positional cloning rice

OsPKpa_1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2018年第11期60卷 1097-1118页

作者： Yue Cai Wenwei Zhang Jie Jin Xiaoming Yang Xiaoman You Haigang Yan Liang Wang Jie Chen Jiahuan Xu Weiwei Chen Xingang Chen Jing Ma Xiaojie Tang Fei Kong Xiaopin Zhu Guoxiang Wang Ling Jiang William Terzaghi Chunming Wang Jianmin Wan State key laboratory for crop genetics and germplasm enhancement Jiangsu Plant Gene Engineering Research CenterNanjing AgriculturalUniversityNanjing 210095China National key Facility for crop gene resources and genetic Improvement Institute of Crop ScienceChinese Academy of AgriculturalSciencesBeijing 100081China Department of Biology Wilkes UniversityWilkes-BarrePA 18766USA

Pyruvate kinase （PK） is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice （Oryza sativa） mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpα1, which encodes a plastidic isoform of PK （PKp）. OsPKpα1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpα1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, at the early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein- protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts.

关键词： Figure OsPKpa1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm

Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat

在线全文

学校读者我要写书评

暂无评论

The crop Journal 2020年第6期8卷 943-952页

作者： Wei Zhang Huifang Li Liya Zhi Qiannan Su Jiajia Liu Xiaoli Ren Deyuan Meng Na Zhang Jun Ji Xueyong Zhang Junming Li Center for Agricultural resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022HebeiChina key laboratory of crop gene resources and germplasm enhancement Ministry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Hebei University of Economics and Business Shijiazhuang 050061HebeiChina State key laboratory of Plant Cell and Chromosome Engineering Chinese Academy of SciencesBeijing 100101China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

The TaGS3 homoeologous genes(homoeologs)located on chromosomes 7 A,4 A,and7 D in hexaploid wheat were *** expression analysis of the three Ta GS3 homoeologs revealed that the expression levels of TaGS3-4 A and TaGS3-7 D in developing grains were higher than that of TaGS3-7 *** evidence showed that Ta GS3 was a negative regulator of grain weight and grain *** polymorphic sites and five haplotypes were detected in TaGS3-4 *** molecular markers were developed to distinguish the five *** analysis using 260 accessions from Chinese wheat mini-core collection(MCC)indicated that TaGS3-4 A affected thousand grain weight(TGW)and grain length(GL).HAP-4 A-1 and HAP-4 A-2 were favorable haplotypes that increased TGW and GL and had undergone strong selection during domestication of *** addition,interaction of the TaGS3-4 A and TaGS3-7 D homoeologs had significant additive effects on the grain ***-4 A-1/Hap-7 D-2 was the best haplotype combination in increasing TGW and *** frequencies and geographic distributions of favorable TaGS3 haplotypes among 1388 wheat accessions from worldwide sources provided clues for selection of yield-related *** findings demonstrated that TaGS3-4 A had significant effects on TGW and ***-assisted selection of HAP-4 A-1/2 combined with HAP-7 D-2 has potential to increase wheat yields.

关键词： TaGS3 Grain yield Haplotype MAS Triticum aestivum

Asymmetric evolution and domestication in allotetraploid cotton (Gossypium hirsutum L.)

在线全文

学校读者我要写书评

暂无评论

The crop Journal 2017年第2期5卷 159-165页

作者： Lei Fang Xueying Guan Tianzhen Zhang State key laboratory of crop genetics and germplasm enhancement Cotton Hybrid R&D Engineering Center (the Ministry of Education)Nanjing Agricultural University Nanjing 210095 China

Polyploidy plays a major role in genome evolution,which corresponds to environmental changes over millions of *** mechanisms of genome evolution,particularly during the process of domestication,are of broad interest in the fields of plant science and crop *** cotton is derived from the hybridization and polyploidization of its ancient A and D diploid *** a result,cotton is a model for polyploid genome evolution and crop *** explore the genomic mysteries of allopolyploid cotton,we investigated asymmetric evolution and domestication in the A and D ***,more structural rearrangements have been characterized in the A subgenome than in the D ***,more transposable elements,a greater number of lost and disrupted genes,and faster evolution have been identified in the A *** contrast,the centromeric retroelement(RT-domain related) sequence of tetraploid cotton derived from the D subgenome progenitor was found to have invaded the A subgenome centromeres after allotetrapolyploid *** there is no genome-wide expression bias between the subgenomes,as with expression-level alterations,gene expression bias of homoeologous gene pairs is widespread and varies from tissue to ***,there are more positively selected genes for fiber yield and quality in the A subgenome and more for stress tolerance in the D subgenome,indicating asymmetric *** review highlights the asymmetric subgenomic evolution and domestication of allotetraploid cotton,providing valuable genomic resources for cotton research and enhancing our understanding of the basis of many other allopolyploids.

关键词： Polyploidy Allotetraploid cotton Asymmetric evolution Asymmetric domestication

Identification and fine mapping of two blast resistance genes in rice cultivar 93-11

在线全文

学校读者我要写书评

暂无评论

The crop Journal 2013年第1期1卷 2-14页

作者： Cailin Lei Kun Hao Yilong Yang Jian Ma Shuai Wang Jiulin Wang Zhijun Cheng Shasha Zhao Xin Zhang Xiuping Guo Chunming Wang Jianmin Wan Institute of crop Science Chinese Academy of Agricultural SciencesThe National Key Facility for Crop Gene Resources and Genetic Improvement key laboratory of crop genetics and germplasm enhancement Jiangsu Provincial Center of Plant Gene Engineering Nanjing Agricultural University

Rice blast, caused by Magnaporthe oryzae, is a major disease of rice almost worldwide. The Chinese indica cultivar 93-11 is resistant to numerous isolates of the blast fungus in China, and can be used as broad-spectrum resistance resource, particularly in japonica rice breeding programs. In this study, we identified and mapped two blast resistance genes, Pi60(t) and Pi61(t), in cv. 93-11 using F2 and F3 populations derived from a cross between the susceptible cv. Lijiangxintuanheigu(LTH) and resistant cv. 93-11 and inoculated with M. oryzae isolates from different geographic origins. Pi60(t) was delimited to a 274 kb region on the short arm of chromosome 11, flanked by InDel markers K1-4 and E12 and cosegregated with InDel markers B1 and Y10. Pi61(t) was mapped to a 200 kb region on the short arm(near the centromere) of chromosome 12, flanked by InDel markers M2 and S29 and cosegregating with InDel marker M9. In the 274 kb region of Pi60(t), 93-11 contains six NBS-LRR genes including the two Pia/ PiCO39 alleles(BGIOSGA034263 and BGIOSGA035032) which are quite close to the two Pia/ PiCO39 alleles(SasRGA4 and SasRGA5) in Sasanishiki and CO39, with only nine amino acids differing in the protein sequences of BGIOSGA035032 and SasRGA5. In the 200 kb region of Pi61(t), 93-11 contains four NBS-LRR genes, all of which show high identities in protein sequence with their corresponding NBS-LRR alleles in susceptible cv. Nipponbare. Comparison of the response spectra and physical positions between the target genes and other R genes in the same chromosome regions indicated that Pi60(t) could be Pia/PiCO39 or its allele, whereas Pi61(t) appears to be different from Pita, Pita-2, Pi19(t), Pi39(t) and Pi42(t) in the same R gene cluster. DNA markers tightly linked to Pi60(t) and Pi61(t) will enable marker-assisted breeding and map-based cloning.

关键词： Oryza sativa Rice blast Magnaporthe oryzae Resistance gene gene mapping

Nuclear encoded elongation factor EF-Tu is required for chloroplast development in rice grown under low-temperature conditions

在线全文

学校读者我要写书评

暂无评论

Journal of genetics and Genomics 2022年第5期49卷 502-505页

作者： Liang Cai Zongkai Liu Long Cai Xiaofeng Yan Yuan Hu Benyuan Hao Zhuang Xu Yunlu Tian Xi Liu Linglong Liu Ling Jiang Shirong Zhou Jianmin Wan State key laboratory for crop genetics and germplasm enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjingJiangsu 210095China National key Facility for crop gene resources and genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

As a thermophilic crop that originated in tropical and subtropical areas, rice is sensitive to low temperatures, particularly during the early seedling stage. Cold stress at the seedling stage inhibits chlorophyll biosynthesis and causes oxidative damage, resulting in death(Zhao et al., 2020). Investigation of the mechanism of chloroplast development in rice seedlings under low-temperature conditions is important for breeding varieties with increased cold resistance.

关键词： seedling breeding originated