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NPR1 as a transgenic crop protection strategy in horticultural species

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Horticulture Research 2018年第1期5卷 751-761页

作者： Katchen Julliany P.Silva Nasser Mahna Zhonglin Mou Kevin M.Folta Horticultural Sciences Department University of FloridaGainesvilleFL32611USA Department of Horticultural Sciences University of TabrizTabrizIran Department of Microbiology and Cell Science University of FloridaGainesvilleFL32611USA Graduate Program in Plant Molecular and Cellular biology University of FloridaGainesvilleFL32611USA

The NPR1(NONEXPRESSOR OF PATHOGENESIS RELATED GENES1)gene has a central role in the long-lasting,broad-spectrum defense response known as systemic acquired resistance(SAR).When overexpressed in a transgenic context in Arabidopsis thaliana,this gene enhances resistance to a number of biotic and abiotic *** position as a key regulator of defense across diverse plant species makes NPR1 a strong candidate gene for genetic engineering disease and stress tolerance into other ***-value horticultural crops face many new challenges from pests and pathogens,and their emergence exceeds the pace of traditional breeding,making the application of NPR1-based strategies potentially useful in fruit and vegetable ***,plants overexpressing NPR1 occasionally present detrimental morphological traits that make its application less *** practical utility of NPR-based approaches will be a balance of resistance gains versus other *** this review,we summarize the progress on the understanding of NPR1-centered applications in horticultural and other crop *** also discuss the effect of the ectopic expression of the *** NPR1 gene and its orthologs in crop plants and outline the future challenges of using NPR1 in agricultural applications.

关键词： NPR1 breeding attractive

Regularized quantile regression for SNP marker estimation of pig growth curves

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Journal of Animal Science and Biotechnology 2017年第4期8卷 824-832页

作者： L.M.A.Barroso M.Nascimento A.C.C.Nascimento1 F.F.Silva N.V.L.Serao C.D.Cruz M.D.V.Resende F.L.Silva C.F.Azevedo P.S.Lopes S.E.F.Guimaraes Department of Statistics Federal University of Vicosa Av. P H Rolfs s/nUniversity Campus Vicosa MG 36570-000 Brazil Department of Animal Science Federal University of Vicosa Av. P H Rolfs s/n University CampusVicosa MG 36570-000 Brazil Department of Animal Science Iowa State University Kildee Hall 50011 Ames Iowa USA Department of General biology Federal University of Vicosa Av. P H Rolfs s/n University CampusVicosa MG 36570-000 Brazil Embrapa Forestry Estrada da Ribeira km 111Colombo PR Brazil. Department of Plant Science Federal University of Vicosa Av. P H Rolfs s/n University Campus Vicosa MG 36570-000 Brazil

Background: Genomic growth curves are general y defined only in terms of population mean; an alternative approach that has not yet been exploited in genomic analyses of growth curves is the Quantile Regression(QR). This methodology allows for the estimation of marker effects at different levels of the variable of interest. We aimed to propose and evaluate a regularized quantile regression for SNP marker effect estimation of pig growth curves, as well as to identify the chromosome regions of the most relevant markers and to estimate the genetic individual weight trajectory over time(genomic growth curve) under different quantiles(levels).Results: The regularized quantile regression(RQR) enabled the discovery, at different levels of interest(quantiles), of the most relevant markers al owing for the identification of QTL regions. We found the same relevant markers simultaneously affecting different growth curve parameters(mature weight and maturity rate): two(ALGA0096701 and ALGA0029483)for RQR(0.2), one(ALGA0096701) for RQR(0.5), and one(ALGA0003761) for RQR(0.8). Three average genomic growth curves were obtained and the behavior was explained by the curve in quantile 0.2, which differed from the ***: RQR allowed for the construction of genomic growth curves, which is the key to identifying and selecting the most desirable animals for breeding purposes. Furthermore, the proposed model enabled us to find, at different levels of interest(quantiles), the most relevant markers for each trait(growth curve parameter estimates) and their respective chromosomal positions(identification of new QTL regions for growth curves in pigs). These markers can be exploited under the context of marker assisted selection while aiming to change the shape of pig growth curves.

关键词： Genome association Growth curve Pig QTL Regularized quantile regression

马铃薯StPEBP基因家族成员鉴定及其在块茎中的表达分析

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中国马铃薯 2022年第2期36卷 97-110页

作者：张文哲荐红举尚丽娜张梅花王季春 Ahmed Mahmoud 吕典秋西南大学农学与生物科技学院重庆400715 薯类生物学与遗传育种重庆市重点实验室重庆400715 Department of Vegetable Crops Faculty of AgricultureCairo UniversityGiza 12613Egypt

马铃薯作为世界上主要的粮食作物之一,其营养价值高,抗逆性强,在全球各地广泛种植。磷脂酰乙醇胺结合蛋白(Phosphatidyl ethanolamine-binding protein,PEBP)家族成员StSP6A和StSP5G在块茎形成中起着重要作用,但其他StPEBP家族基因成员... 详细信息

马铃薯作为世界上主要的粮食作物之一,其营养价值高,抗逆性强,在全球各地广泛种植。磷脂酰乙醇胺结合蛋白(Phosphatidyl ethanolamine-binding protein,PEBP)家族成员StSP6A和StSP5G在块茎形成中起着重要作用,但其他StPEBP家族基因成员在块茎形成中的作用尚未解析。研究利用拟南芥PEBP基因家族蛋白序列在马铃薯全基因组内进行Blastp比对,并对马铃薯StPEBP基因家族的保守基序、基因结构、顺式作用元件、染色体位置、共线性关系以及该家族在不同组织中特别是块茎发育中的表达模式进行分析。马铃薯StPEBP基因家族成员共有10个基因,该家族大部分成员含有4个外显子和3个内含子以及保守基序motif 5。系统进化分析表明StPEBP家族基因分为4个亚家族。顺式作用元件预测发现StPEBP家族基因含有大量光响应、激素响应和防御及应激反应元件。染色体定位分析发现StPEBP家族基因定位在6条染色体上,共线性分析检测到马铃薯中2对StPEBP基因间为重复基因。非同义/同义置换率的比率说明马铃薯StPEBP基因家族受到了纯化选择。qRT-PCR结果表明,StPEBP家族基因是诱导马铃薯块茎形成的潜在调控分子。研究结果可为进一步开展马铃薯结薯性状基因的筛选提供理论参考。

关键词：马铃薯块茎形成 StPEBP 基因家族表达

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杂交枫香胚性愈伤组织遗传转化体系研究

北京林业大学学报 2021年第8期43卷 9-17页

作者：江帅菲崔莹赵瑞瑞齐帅征 Kong Lisheng 赵健李珊珊张金凤林木育种国家工程实验室林木花卉遗传育种教育部重点实验室国家林业局树木花卉育种与生物工程重点实验室北京林业大学生物科学与技术学院北京100083 Department of biology University of VictoriaCanada Victoria BC V8P5C2 神舟绿鹏农业科技有限公司北京101105

【目的】杂交枫香是我国重要的用材和观赏树种资源,但其遗传转化体系尚未建立。建立杂交枫香遗传转化体系为杂交枫香性状改良和基因功能研究提供了方法。【方法】本研究基于杂交枫香高效的体细胞胚胎发生技术,用根癌农杆菌介导的遗传转... 详细信息

【目的】杂交枫香是我国重要的用材和观赏树种资源,但其遗传转化体系尚未建立。建立杂交枫香遗传转化体系为杂交枫香性状改良和基因功能研究提供了方法。【方法】本研究基于杂交枫香高效的体细胞胚胎发生技术,用根癌农杆菌介导的遗传转化法对其胚性愈伤组织进行遗传转化,对潮霉素选择压、菌液浓度、侵染时间、共培养时间、以及共培养温度等影响因素采用正交试验等设计进行了研究。【结果】潮霉素对胚性愈伤组织的最小致死质量浓度为10 mg/L;获得最多Gus阳性斑点数的组合的菌液OD600为0.8,共培养时间为3 d,侵染时间为10 min,共培养温度为25℃;获得最多转基因阳性愈伤组织的组合的菌液OD600为0.2,共培养时间为2 d,侵染时间为10 min,共培养温度为23℃;且通过极差分析,最优处理组合的共培养时间为2 d,菌液OD600为0.2,侵染时间为20 min,共培养温度为23℃。【结论】经分子鉴定,共获得210个转基因阳性愈伤组织,初步建立了农杆菌介导的杂交枫香遗传转化体系,为阔叶树种愈伤组织的遗传转化提供了更多的可行性依据。

关键词：杂交枫香胚性愈伤组织根癌农杆菌遗传转化

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苹果种质资源火疫病抗性鉴定评价与筛选

植物遗传资源学报 2022年第6期23卷 1682-1695页

作者：王大江高源张玉刚张校立孙思邈路翔郭绍霞 Khurshid STurakulov 李连文王昆中国农业科学院果树研究所/农业农村部园艺作物种质资源利用重点实验室辽宁兴城125100 青岛农业大学山东青岛266109 新疆农业科学院园艺作物研究所乌鲁木齐830091 石河子大学农学院/特色果树栽培生理与种质资源利用兵团重点实验室新疆石河子832000 Institute of genetics and Plant Experimental biology Academy Sciences of UzbekistanUzbekistan Tashkent

以488份苹果种质资源为试材,利用嫩叶和嫩枝离体接种方法鉴定评价苹果属栽培品种、野生种和古老栽培品种的火疫病抗性。结果表明,嫩叶和嫩枝接种结果存在较大差异,嫩枝接种的抗性资源明显多于嫩叶接种。不同种、不同来源、不同系谱的苹... 详细信息

以488份苹果种质资源为试材,利用嫩叶和嫩枝离体接种方法鉴定评价苹果属栽培品种、野生种和古老栽培品种的火疫病抗性。结果表明,嫩叶和嫩枝接种结果存在较大差异,嫩枝接种的抗性资源明显多于嫩叶接种。不同种、不同来源、不同系谱的苹果资源火疫病抗性均存在较大差异。嫩叶接种显示苹果栽培品种和野生种的抗病资源比例均高于古老栽培品种;旭系和金冠系抗性资源比例较高;不同来源的189份塞威士苹果评价结果显示,高抗资源主要来自乌兹别克斯坦和中国新疆新源。嫩枝接种显示栽培品种的抗性表现与嫩叶接种相似;塞威士苹果则存在差异,来自乌兹别克斯坦和中国新疆巩留的抗性类型比例较高。嫩叶和嫩枝接种结果一致的187份资源分析,发现苹果栽培品种抗性资源比例较高;旭系品种的抗性较强;抗病的塞威士苹果主要来自乌兹别克斯坦和中国新疆新源。筛选高抗资源8份,苹果栽培品种6份和塞威士苹果2份。6份苹果栽培品种可作为鲜食种质创制和品种选育亲本,2份塞威士苹果既可作为鲜食也可用作砧木品种选育的基础材料。

关键词：苹果种质资源火疫病离体接种评价筛选