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Asian Herpetological Research 2014年第1期5卷 1-11页

作者： Daode YANG Jianping JIANG Youhui SHEN Dongbo FEI Institute of Wildlife Conservation Central South University of Forestry and Technology Chengdu Institute of biology Chinese Academy of Sciences College of Life Sciences Hunan Normal University

We describe a new species from the family Salamandridae from northeastern Hunan Province of China based on morphological and molecular genetic *** new species,Tylototriton liuyangensis,is a member of the *** group,and shares a number of similarities with *** and *** dorsal surface of the new Tylototriton species is completely black,with nodule-like warts distributed evenly along the lateral margin of dorsal body,and there is no transverse striae between the *** are several diagnostic characters of the new species that differ from those of *** and *** as follows：（1）the interorbital space（IOS）is comparatively wide;（2）there is a bony ridge present on the dorsal head surface,running along the upper eyelids;（3）the space between axilla and groin（AGS）is comparatively large,with the mean male AGS approximately equal to 50%of the snout-vent length（SVL）and the mean female AGS being 53%of SVL;and（4）there is a small genital armature on the anterior angle of labial folds present inside the anal fissure of adult males during the breeding *** result from the molecular phylogenetic analysis of the genus Tylototriton（including the type specimen of the new species）based on the complete DNA sequence of the mitochondrial ND2 gene（1035 bp）indicated that the new species was similar to ***,***,and ***,but formed an independent *** result was consistent with the morphological analysis above,supporting that the Liuyang Population represented a distinct species.

关键词： new species Tylototriton liuyangensis Salamandridae taxonomy Liuyang Daweishan Provincial Nature Reserve of Hunan Province in China

Retrotransposon-mediated DELLA transcriptional reprograming underlies semi-dominant dwarfism in foxtail millet

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The Crop Journal 2019年第4期7卷 458-468页

作者： Meicheng Zhao Hui Zhi Xue Zhang Guanqing Jia Xianmin Diao Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China Center for Agricultural Research Resources Institute of Genetics and Developmental Biology Chinese Academy of Sciences Shijiazhuang 050021 Hebei China

Retrotransposons account for a large proportion of the genome and genomic variation, and play key roles in creating novel genes and diversifying the genome in many eukaryotic species. Although retrotransposons are abundant in plants, their roles had been underestimated because of a lack of research. Here, we characterized a gibberellin Acid (GA)-insensitive dwarf mutant, 84133, in foxtail millet. Map-based cloning revealed a 5.5-kb Copia-like retrotransposon insertion in DWARF1 (D1), which encodes a DELLA protein. Transcriptional analysis showed that the Copia retrotransposon mediated the transcriptional reprogramming of D1 leading to a novel N-terminal-deleted truncated DELLA transcript that was putatively driven by Copia's LTR, namely D1-TT, and another chimeric transcript. The presence of D1-TT was confirmed by protein immunodetection analysis. Furthermore, D1-TT protein was resistant to GA3 treatment compared with the intact DELLA protein due to its inability to interact with the GA receptor, SiGID1. Overexpression of D1-TT in foxtail millet resulted in dwarf plants, confirming that it determines the dwarfism of 84133. Thus, our study documents a rare instance of long terminal repeat (LTR) retrotransposon-mediated transcriptional reprograming in the plant kingdom. These results shed light on the function of LTR retrotransposons in generating new gene functions and genetic diversity.

关键词： Retrotransposon Transcriptional reprogramming DELLA Dwarf breeding Foxtail millet (Setaria italica)

Wheat stripe rust resistance gene Yr24/Yr26:A retrospective review

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Identification and validation of major QTL for grain size and weight in bread wheat(Triticum aestivum L.)

The Crop Journal 2018年第4期 321-329页

作者： RobertMcIntosh JingmeiMu DejunHan ZhenshengKang Plant breeding Institute Cobbitty，University of Sydney State key laboratory of Crop Stress biology in Arid Areas，College of Agronomy，Northwest A&F University College of Agronomy，Northwest A&F University College of Plant Protection，Northwest A&F University

The objective of this review is to describe events in China and elsewhere that are related to the discovery, genetic identification, use, and ultimate break-down of a single wheat gene for resistance to stripe rust, namely Yr24/Yr26. In our retrospective analysis there was an early assumption of at least three genes at or near the locus, which caused an erroneous presumption of genetic diversity for resistance. It is an example of another boom and bust cycle in plant breeding with races virulent to Yr26(V26 races) now being the majority race group in the Chinese Pst population. We have attempted to present our story in a historical and personal context demonstrating research inputs from different national and international groups, as well as some significant contemporary side issues. It covers the period from the late 1980 s to 2017, during which significant rapid advances in the molecular biology of host: pathogen genetics occurred. We attempt to describe both successes and drawbacks in our work.

关键词：抗条锈病单基因植物学预防措施

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The Crop Journal 2023年第2期11卷 564-572页

作者： Guangsi Ji Zhibin Xu Xiaoli Fan Qiang Zhou Liangen Chen Qin Yu Simin Liao Cheng Jiang Bo Feng Tao Wang Chengdu Institute of biology Chinese Academy of SciencesChengdu 610041SichuanChina University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Grain size and weight are key components of wheat *** of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was constructed to detect QTL for thousand-grain weight(TGW),grain length(GL)and grain width(GW)across eight *** regions associated with grain size and grain weight were identified on chromosomes 4A and 6A using bulked segregant exome sequencing(BSE-Seq)*** constructing genetic maps,six major QTL detected in at least four individual environments and in best linear unbiased estimator(BLUE)datasets,explained 7.50%-23.45%of the phenotypic *** for ***-4A,the other five QTL were co-located in two regions,namely QTgw/***-4A and QTgw/Gw/*** of these QTL were *** QTgw/Gw/***-6A,QTgw/***-4A and ***-4A had no effect on grain number per spike(GNS).The QTL were validated in a second cross using Kompetitive Allele Specific PCR(KASP)*** QTgw/***-4A was probably a novel locus,it and the KASP markers reported here can be used in wheat ***4A03G0191200 was predicted to be potential candidate gene for QTgw/***-4A based on the sequence differences,spatiotemporal expression patterns,gene annotation and haplotype *** findings will be useful for fine mapping and for marker-assisted selection in wheat grain yield improvement.

关键词： BSE-Seq Thousand-grain weight Grain length Grain width QTL mapping

Identification of blast-resistance loci through genome-wide association analysis in foxtail millet(Setaria italica (L.)Beauv.)

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Journal of Integrative agriculture 2021年第8期20卷 2056-2064页

作者： LI Zhi-jiang JIA Guan-qing LI Xiang-yu LI Yi-chu ZHI Hui TANG Sha MA Jin-feng ZHANG Shuo LI Yan-dong SHANG Zhong-lin DIAO Xian-min College of Life Sciences Hebei Normal UniversityShijiazhuang 050024P.R.China Institute of Crop breeding Heilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Plant Protection Heilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China

Blast disease caused by the fungus Magnaporthe grisea results in significant yield losses of cereal crops across the *** date,very few regulatory genes contributing to blast resistance in grass species have been identified and the genetic basis of blast resistance in cereals remains ***,a core collection of foxtail millet(Setaria italica)containing 888 accessions was evaluated through inoculation with the blast strain HN-1 and a genome-wide association study(GWAS)was performed to detect regulators responsible for blast disease resistance in foxtail *** phenotypic variation of foxtail millet accessions inoculated with the blast strain HN-1 indicated that less than 1.60% of the samples were highly resistant,35.25% were moderately resistant,57.09% were moderately susceptible,and 6.08% were highly *** geographical pattern of blast-resistant samples revealed that a high proportion of resistant accessions were located in lower latitude regions where the foxtail millet growing season has higher rain *** 720000 SNP markers covering the Setaria genome,GWAS showed that two genomic loci from chromosomes 2 and 9 were significantly associated with blast disease resistance in foxtail ***,eight putative genes were identified using rice blast-related transcriptomic *** results of this work lay a foundation for the foxtail millet blast resistance biology and provide guidance for breeding practices in this promising crop species and other cereals.

关键词： foxtail millet blast resistance core collection GWAS

Development of EST-SSR primers and their practicability test for Laminaria

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Acta Oceanologica Sinica 2011年第3期30卷 112-117页

作者： WANG Guoliang TAN Xiaolong SHEN Junling LI Jian ZHANG Lei SUN Jianwei WANG Bin WENG Manli LIU Tao College of Marine Life Science Ocean University of China Qingdao 266003 China Institute of genetics and Developmental biology Chinese Academy of Sciences Beijing 100101 China College of Horticulture Qingdao Agricultural University Qingdao 266109 China

Laminaria （Saccharina） is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria （971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases） with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found. From the 254 SSRs, sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most （more than 80%） of the 12 Laminara DNA templates. genetic diversity study of 12 Laminaria lines, which were widely used in breeding and economic cultivation in China, was performed based on the obtained SSR data.

关键词： Laminaria EST-SSRs germplasm identification polymorphism analysis

Phased,chromosome-scale genome assemblies of tetraploid potato reveal a complex genome,transcriptome,and predicted proteome landscape underpinning genetic diversity

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Molecular Plant 2022年第3期15卷 520-536页

作者： Genevieve Hoopes Xiaoxi Meng John P.Hamilton Sai Reddy Achakkagari Fernanda de Alves Freitas Guesdes Marie E.Bolger Joseph J.Coombs Danny Esselink Natalie R.Kaiser Linda Kodde Maria Kyriakidou Brian Lavrijssen Natascha van Lieshout Rachel Shereda Heather K.Tuttle Brieanne Vaillancourt Joshua C.Wood Jan Mde Boer Nolan Bornowski Peter Bourke David Douches Herman Jvan Eck Dave Ellis Max J.Feldman Kyle M.Gardner Johannes C.P.Hopman Jiming Jiang Walter S.De Jong Joseph C.Kuhl Richard G.Novy Stan Oome Vidyasagar Sathuvalli Ek Han Tan Remco A.Ursum M.Isabel Vales Kelly Vining Richard G.F.Visser Jack Vossen G.Craig Yencho Noelle L.Anglin Christian W.B.Bachem Jeffrey B.Endelman Laura M.Shannon Martina V.Stromvik Helen H.Tai Bjorn Usadel C.Robin Buell Richard Finkers Department of Plant biology Michigan State UniversityEast LansingMI 48824USA Department of Horticultural Science University of MinnesotaSt.PaulMN 55108USA Department of Plant Science McGill UniversitySainte-Anne-de-BellevueQC H9X 3V9Canada Plant breeding Wageningen University&ResearchPlant Breeding6708 PB Wageningenthe Netherlands IBG-4 Bioinformatics Forschungszentrum JülichWilhelm Johnen Str52428 JülichGermany Department of Plant Soiland Microbial SciencesMichigan State UniversityEast LansingMI 48824USA Bayer Crop Science WoodlandCA 95695USA Averis Seeds B.V. 9640 AA Veendamthe Netherlands International Potato Center 1895 Avenida La MolinaLimaPeru USDA-ARS ProsserWA 99350USA agriculture and Agri-Food Canada Fredericton Research and Development Centre FrederictonNB E3B 4Z7Canada School of Integrative Plant Science Cornell UniversityIthacaNY 14853-1901USA Department of Plant Sciences University of IdahoMoscowID 83844USA USDA-ARS Small Grains and Potato Germplasm ResearchAberdeenID 83210USA HZPC Research B.V. Edisonweg 58501 XG Jourethe Netherlands Department of Crop and Soil Science Oregon State UniversityHermistonOR 97838USA School of biology and Ecology University of Maine5735 Hitchner Hall OronoME 04469USA Department of Horticultural Sciences Texas A&M UniversityCollege StationTX 77843-2133USA Department of Horticulture Oregon State UniversityCorvallisOR 97331USA Department of Horticultural Science North Carolina State UniversityRaleighNC 27695-7609USA Department of Horticulture University of Wisconsin-MadisonMadisonWI 53706USA Department of Horticulture Michigan State UniversityEast LansingMI 48824USA Institute for Biological Data Science Heinrich Heine UniversityDusseldorf40225 DusseldorfGermany Plant Resilience Institute Michigan State UniversityEast LansingMI 48824USA Present address:GenNovation B.V. Wageningenthe Netherlands

Cultivated potato is a clonally propagated autotetraploid species with a highly heterogeneous *** assemblies of six cultivars including two chromosome-scale phased genome assemblies revealed extensive allelic diversity,including altered coding and transcript sequences,preferential allele expression,and structural variation that collectively result in a highly complex transcriptome and predicted proteome,which are distributed across the homologous *** species contribute to the extensive allelic diversity in tetraploid cultivars,demonstrating ancestral introgressions predating modern breeding *** a clonally propagated autotetraploid that undergoes limited meiosis,dysfunctional and deleterious alleles are not purged in tetraploid *** a quarter of the loci bore mutations are predicted to have a high negative impact on protein function,complicating breeder’s efforts to reduce genetic *** StCDF1 locus controls maturity,and analysis of six tetraploid genomes revealed that 12 allelic variants of StCDF1 are correlated with maturity in a dosage-dependent *** of the complexity of the tetraploid potato genome with its rampant structural variation and embedded deleterious and dysfunctional alleles will be key not only to implementing precision breeding of tetraploid cultivars but also to the construction of homozygous,diploid potato germplasm containing favorable alleles to capitalize on heterosis in F1 hybrids.

关键词： potato allele diversity phased assembly polyploid pan-genome wild introgressions

Phenotypic diversity of rapeseed accessions from different geographic locations

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Oil Crop Science 2016年第1期1卷 9-20页

作者： Hongyun TIAN Wei LI Vratislav KUCERAC Michael J.STAMM Shengwu HU State key laboratory of Crop Stress biology in Arid Areas and College of Agronomy Northwest A&F UniversityYangling 112100 P. R. China Crop Designing Centre Henan Academy of Agricultural Sciences Zhengzhou 450002 P. R. China Crop Research Institute Drnovska 507 Prague 6 16106 Czech Republic Department of Agronomy Kansas State University Manhattan KS 66506 USA

It is essential to evaluate agronomic and quality traits when using elite lines in rapeseed （Brassica napus L. ） breeding. 92 rapeseed accessions （44 Chinese, 22 European, 16 USA and 10 Ca-nadian） were evaluated by 16 agronomic and quality traits in Northern China. Analysis of variance among the 92 accessions revealed significant differences on 13 traits. The 13 traits were plant height, setting po-sition of the first primary branch, silique number （ on terminal raceme） , silique density, siliques per plant, seeds per silique, thousand seed weight, seed yield per plant, flowering time, final flowering, ma-turity time, cold mortality, and oil content. Other 3 traits were not significant （primary branches per plant, terminal raceme length and seed protein） . Cluster analysis revealed that 92 accessions were clus-tered into 3 major groups. The 1st group had 10 Canadian and 5 Chinese. The 2nd group had 33 acces-sions from China and 5 from Europe. The 3rd group had 39 accessions from Europe and the USA. For ag-ronomy and quality traits, 45. 73% genetic variation attributed to differences among populations of geo-graphic regions, and 54. 27% attributed to differences within geographic regions. In summary, the a-broad accessions were distinguished from domestic ones, and could be used as important germplasm re-sources for enriching the genetic background of Chinese rapeseed, and vice versa.

关键词： Brassica napus L. agronomic traits quality traits genetic diversity

Shortwave Radiation Calculation for Forest Plots Using Airborne LiDAR Data and Computer Graphics

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Plant Phenomics 2022年第1期4卷 175-195页

作者： Xinbo Xue Shichao Jin Feng An Huaiqing Zhang Jiangchuan Fan Markus P.Eichhorn Chengye Jin Bangqian Chen Ling Jiang Ting Yun School of Information Science and Technology Nanjing Forestry UniversityNanjing 210037China Forestry College Nanjing Forestry UniversityNanjing 210037China Plant Phenomics Research Centre Academy for Advanced Interdisciplinary StudiesCollaborative Innovation Centre for Modern Crop Production Cosponsored by Province and MinistryNanjing Agricultural UniversityNanjing 210095China Chinese Academy of Tropical Agricultural Sciences Ministry of AgricultureRubber Research InstituteDanzhou Investigation and Experiment Station of Tropical CropsDanzhouChina Research Institute of Forestry Resource Information Techniques Chinese Academy of ForestryBeijing 100091China National Engineering Research Center for Information Technology in agriculture Beijing 100097China School of Biological Earth and Environmental SciencesUniversity College CorkDistillery FieldsNorth MallCork T23 N73KIreland Environmental Research Institute University College CorkLee RoadCork T23 XE10Ireland

Forested environments feature a highly complex radiation regime,and solar radiation is hindered from penetrating into the forest by the 3D canopy structure;hence,canopy shortwave radiation varies spatiotemporally,seas... 详细信息

关键词： Forest forest hence