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Assessment of genetic diversity in the forest musk deer (Moschus berezovskii) using microsatellite and AFLP markers

Chinese Science Bulletin 2011年第24期56卷 2565-2569页

作者： ZHAO ShaSha CHEN Xuan WAN QiuHong key laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and state Conservation Center for Gene Re-sources of Endangered Wildlife College of Life Sciences Zhejiang University Hangzhou 310058 China Sichuan Provincial laboratory for Natural resources Protection and Sustainable utilization Chengdu 610015 China

Microsatellite genotyping and amplified fragment length polymorphism (AFLP) techniques are often utilized in studies of conservation genetics of endangered animals. To select a more effective marker system for conserving the endangered forest musk deer, we used microsatellite and AFLP markers to estimate levels of genetic diversity of two populations, the pure mother Jinfengshan (JFS) group and the offspring Baisha (BS) group with introduction of new blood. It was expected that JFS would pos- sess significantly higher genetic variability than BS if it is assumed that no new deer were introduced into BS. The results revealed that the 15 microsatellite markers produced 141 alleles and the 22 AFLP primer combinations yielded 908 polymorphic bands, showing that AFLPs had higher resolving power than microsatellites. Nonetheless, compared with other deer, both marker systems indicated that the forest musk deer contains a relatively high level of genetic variation. The 15 microsatellite loci indicated a similar level of genetic diversity was present in the JFS and BS populations, seemingly suggesting that the new blood obviously elevated the level of genetic diversity in BS. However, 22 suites of AFLP markers yielded a significantly higher level of genetic variation in JFS than that in BS, in contrast to the diversity pattern of microsatellites. In view of the difference in resolving power of these two marker systems, we conclude that gene introgression was limited in the BS population, as revealed by the AFLP markers. Furthermore, from the perspective of whole genome variability, we recommend that the AFLP marker system is more suitable for conservation genetics studies on the forest musk deer.

关键词： AFLP标记遗传多样性微卫星标记林麝扩增片段长度多态性保护遗传学评估遗传变异

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Diurnal and seasonal variations in carbon fluxes in bamboo forests during the growing season in Zhejiang province, China

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Journal of forestry Research 2019年第2期30卷 657-668页

作者： Liang Chen Yuli Liu Guomo Zhou Fangjie Mao Huaqiang Du Xiaojun Xu Pingheng Li Xuejian Li state key laboratory of Subtropical Silviculture Zhejiang A & F University key laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration of Zhejiang Province Zhejiang A & F University School of Environmental and resources Science Zhejiang A & F University

bamboo forest is an important forest type in subtropical China and is characterized by fast growth and high carbon sequestration capacity. However, the dynamics of carbon fluxes during the fast growing period of bamboo shoots and their correlation with environment factors are poorly understood. We measured carbon dioxide exchange and climate variables using open-path eddy covariance methods during the 2011 growing season in a Moso bamboo forest(MB, Phyllostchys edulis) and a Lei bamboo forest(LB, Phyllostachys violascens) in Zhejiang province,China. The bamboo forests were carbon sinks during the growing season. The minimum diurnal net ecosystem exchange(NEE) at MB and LB sites were-0.64 and-0.66 mg C m^(-2) s^(-1), respectively. The minimum monthly NEE, ecosystem respiration(RE), and gross ecosystem exchange(GEE) were-99.3 ± 4.03, 76.2 ±2.46, and^(-1)91.5 ± 4.98 g C m^(-2) month^(-1), respectively,at MB site, compared with-31.8 ± 3.44, 70.4 ± 1.41,and^(-1)57.9 ± 4.86 g C m^(-2) month^(-1), respectively, at LB site. Maximum RE was 92.1 ± 1.32 g C m^(-2) month^(-1) at MB site and 151.0 ± 2.38 g C m^(-2) month^(-1) at LB *** control factors varied by month during the growing season, but across the whole growing season, NEE and GEE at both sites showed similar trends in sensitivities to photosynthetic active radiation and vapor pressure deficit,and air temperature had the strongest correlation with RE at both sites. Carbon fluxes at LB site were more sensitive to soil water content compared to those at MB site. Both onyear(years when many new shoots are produced) and offyear(years when none or few new shoots are produced)should be studied in bamboo forests to better understand their role in global carbon cycling.

关键词： bamboo forest Eddy covariance Carbon fluxes Carbon sequestration

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Neottia wuyishanensis(Orchidaceae:Neottieae),a new species from Fujian,China

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Plant Diversity 2021年第5期43卷 426-431页

作者： Bing-Hua Chen Xiao-Hua Jin College of Life Sciences Fujian Normal UniversityFuzhou 350117China state key laboratory of Systematic and Evolutionary Botany and Herbarium Institute of BotanyChinese Academy of SciencesBeijing 100093China The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the state Oceanic administration Fujian Key Laboratory of Special Marine Bioresource Sustainable UtilizationSouthern Institute of OceanographyCollege of Life SciencesFujian Normal UniversityFuzhou 350117China

A new species,Neottia wuyishanensis(Neottieae,Orchidaceae),is described and illustrated from Wuyishan,Fujian,eastern China,based on morphological characters and molecular *** phylogenetics indicate that *** is nested within the clade formed by Neottia fugongensis and Neottia ***,*** is similar to *** and ***,but differs from them by having indistinct auricles at the base,and in the shape and size of lobelets.

关键词： China Neottia wuyishanensis New species Orchidaceae

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Ceratopteris chunii and Ceratopteris chingii(Pteridaceae),two new diploid species from China,based on morphological,cytological,and molecular data

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Plant Diversity 2022年第3期44卷 300-307页

作者： Jun-Hao Yu Rui Zhang Qiao-Ling Liu Fa-Guo Wang Xun-Lin Yu Xi-Ling Dai Yong-Bo Liu Yue-Hong Yan Eastern China Conservation Centre for Wild Endangered Plant resources Shanghai Chenshan Botanical GardenShanghai 201602China College of Life Science Shanghai Normal UniversityShanghai200234China Shenzhen key laboratory for Orchid Conservation and utilization National Orchid Conservation Center of China and the Orchid Conservation&Research Center of ShenzhenShenzhen518114China Guangdong Provincial key laboratory of Applied Botany South China Botanical GardenChinese Academy of SciencesGuangzhou510650China College of forestry Central South University of Forestry&TechnologyChangshaHunan410004China state Environmental Protection key laboratory of Regional Eco-process and Function Assessment Chinese Research Academy of Environmental SciencesBeijing100012China

Understanding how natural hybridization and polyploidizations originate in plants requires identifying potential diploid ***,cryptic plant species are widespread,particularly in Ceratopteris(Pteridaceae).Identifying Ceratopteris cryptic species with different polyploidy levels is a challenge because Ceratopteris *** high degrees of phenotypic ***,two new cryptic species of Ceratopteris,Ceratopteris chunii and Ceratopteris chingii,are described and *** analyses reveal that each of the new species form a well-supported *** and *** are similar to Ceratopteris gaudichaudii *** and ***,respectively,but distinct from their relatives in the stipe,basal pinna of the sterile leaf or subelliptic shape of the fertile leaf,as well as the spore *** addition,chromosome studies indicate that *** and *** are both *** findings will help us further understand the origin of Ceratopteris polyploids in Asia.

关键词： Ceratopteris Phylogeny Chromosome Taxonomy Cryptic species

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Sequencing of Cultivated Peanut, Arachis hypogaea, Yields Insights into Genome Evolution and Oil Improvement

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Molecular Plant 2019年第7期12卷 920-934页

作者： Xiaoping Chen Qing Lu Hao Liu Jianan Zhang Yanbin Hong Haofa Lan Haifen Li Jinpeng Wang Haiyan Liu Shaoxiong Li Manish K.Pandey Zhikang Zhang Guiyuan Zhou Jigao Yu Guoqiang Zhang Jiaqing Yuan Xingyu Li Shijie Wen Fanbo Meng Shanlin Yu Xiyin Wang Kadambot H.M.Siddique Zhong-Jian Liu Andrew H.Paterson Rajeev K.Varshney Xuanqiang Liang South China Peanut Sub-center of National Center of Oilseed Crops Improvement Guangdong Key Laboratory for Crops Genetic Improvement Crops Research Institute Guangdong Academy of Agricultural Sciences (GAAS) Guangzhou China Fujian Colleges and Universities Engineering Research Institute of Conservation and utilization of Natural Bioresources College of Forestry Fujian Agriculture and Forestry University Fuzhou 350002 China Plant Genome Mapping laboratory University of Georgia Athens USA National Foxtail Millet Improvement Center Minor Cereal Crops Laboratory of Hebei Province Institute of Millet Crops Hebei Academy of Agriculture and Forestry Sciences Shijiazhuang China MolBreeding Biotechnology Co. Ltd. Shijiazhuang China School of Life Sciences and Center for Genomics and Computational Biology North China University of Science and Technology Tangshan China Center of Excellence in Genomics & Systems Biology International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Hyderabad India Shenzhen key laboratory for Orchid Conservation and utilization National Orchid Conservation Center of China and Orchid Conservation and Research Center of Shenzhen Shenzhen China Shandong Peanut Research Institute Shandong Academy of Agricultural Sciences Qingdao China UWA Institute of Agriculture The University of Western Australia Crawley Australia key laboratory of National forestry and Grassland administration for Orchid Conservation and utilization at College of Landscape Architecture Fujian Agriculture and Forestry University Fuzhou 350002 China

Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil and protein. To explore the origins and consequences of tetraploidy, we sequenced the allotetraploid A. hypogaea genome and compared it with the related diploid Arachis duranensis and Arachis ipaensis genomes. We annotated 39 888 A-subgenome genes and 41 526 B-subgenome genes in allotetraploid peanut. The A. hypogaea subgenomes have evolved asymmetrically, with the B subgenome resembling the ancestral state and the A subgenome undergoing more gene disruption, loss, conversion, and transposable element proliferation, and having reduced gene expression during seed development despite lacking genome-wide expression dominance. Genomic and transcriptomic analyses identified more than 2 500 oil metabolism-related genes and revealed that most of them show altered expression early in seed development while their expression ceases during desiccation, presenting a comprehensive map of peanut lipid biosynthesis. The availability of these genomic resources will facilitate a better understanding of the complex genome architecture, agronomically and economically important genes, and genetic improvement of peanut.

关键词： cultivated peanut denovo sequencing comparative genomics genome evolution oil metabolism

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Water Absorption Capacity and Coating Adhesion on Thermally Modified and Not-Modified Spruce Wood(Blue Stained or Free of Blue Stained)

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Journal of Renewable Materials 2023年第12期11卷 4061-4078页

作者： Demiao Chu Redžo Hasanagić Leila Fathi Mohsen Bahmani Miha Humar key Lab of state Forest and Grassland administration on“Wood Quality Improvement&High Efficient utilization” School of Forestry&Landscape ArchitectureAnhui Agricultural UniversityHefei230036China Faculty of Technical Engineering Department of Wood Science and TechnologyUniversity of BihaćBihać7700Bosnia and Herzegovina Department of Natural resources and Earth Science Shahrekord UniversityShahrekord64165478Iran Biotechnical Faculty Department of Wood ScienceUniversity of LjubljanaLjubljana1501Slovenia

This study aimed to investigate the water absorption capacity of thermally modified and non-modified spruce and blue-stained spruce *** wettability of wood depends on various factors,including its type,density,porosity,and surface *** can swell and become distorted when exposed to water or humidity,impacting its structural ***,it is crucial to consider the water and water vapour uptake in the wood when choosing materials for applications that are likely to be exposed to *** moisture absorption tests were conducted to assess water absorption capacity,including short-term and long-term water absorption and water vapour *** results showed a significant difference in the long-term exposure to water,which was related to the density of the *** study examined the influence of thermal treatment on the physical properties of wood and observed significant variations in mass change due to coating,indicating differences in adhesion among different wood ***-treated blue-stained Norway spruce demonstrated higher adhesion(5%–15%)compared to air-treated ***,cohesion tests revealed lower cohesion force in blue-stained Norway spruce(approximately 20%–30%)compared to Norway *** study also used industry-standard tests to investigate the adhesion and cohesion of nano-coatings on wood *** results provided valuable information on the properties of coatings applied to wood,which is vital in protecting and decorating wood while also providing preventive protection against wood pests,weathering,and mechanical *** modification in vacuum involves subjecting the wood to a low-pressure environment to remove air and moisture,allowing for deeper and more uniform penetration of treatment *** contrast,wood modification in air relies on the natural circulation of air to facilitate the absorption of chemical treatments,without the need for a vacuum chamber.

关键词： Wood durability long-term exposure moisture absorption wettability absorption

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Taxonomy and the evolutionary history of Micropeltidaceae

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Fungal Diversity 2019年第4期97卷 393-436页

作者： Xiang-Yu Zeng Hai-Xia Wu Sinang Hongsanan Rajesh Jeewon Ting-Chi Wen Sajeewa S.N.Maharachchikumbura Putarak Chomnunti Kevin D.Hyde International Fungal Research and Development Centre Key Laboratory of Resource Insect Cultivation&Utilization State Forestry AdministrationThe Research Institute of Resource InsectsChinese Academy of ForestryKunming 650224China The Engineering Research Center of the utilization for Characteristic Bio-Pharmaceutical resources in Southwest Guizhou UniversityGuiyang 550025China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Shenzhen key laboratory of Microbial Genetic Engineering College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518000China Shenzhen key laboratory of Laser Engineering College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518000China Department of Health Sciences Faculty of ScienceUniversity of MauritiusReduit 80837Mauritius Department of Crop Sciences College of Agricultural and Marine SciencesSultan Qaboos UniverstyPO Box 8123 Al KhoudOman School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand key laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of Sciences132 Lanhei RoadKunming 650201Chin

Micropeltidaceae species are flyspeck fungi which have been subjected to few systematic *** re-examined 27 genera which were accepted in the Micropeltidaceae and re-described them based on herbaria materials and *** on morphology and phylogenetic investigations,we transfer Micropeltidaceae to a new order,Micropeltidales(Lecanoromycetes).Genera with bluish or greenish upper walls(Dictyopeltella,Dictyothyriella,Dictyothyrina,Dictyothyrium,Haplopeltheca,Micropeltis,Scolecopeltidium and Stomiopeltopsis)are accepted in the new taxonomic concept for Micropeltidaceae.A molecular clock approach estimated the divergence time of the Micropeltidaceae crown group at 130(165–104)Mya,which also supports its rank as an order(diverging from 220–100 Mya).The evolutionary histories between Micropeltidaceae species and host plants are interpreted by cophylogenetic analyses calibrated by their divergence *** result indicates that the diversification of Angiospermae(130–80 Mya)fosters the formation of genera of Micropeltidaceae mainly via cospeciation events,and this codivergent period would be an important reference when establishing generic boundaries of epifoliar fungi.

关键词： Codivergence Cophylogeny Dating Lecanoromycetes Micropeltidales

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Research on Ecological Restoration Technology in Arid or Semi-arid Areas from the Perspective of the Belt and Road Initiative

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Journal of resources and Ecology 2022年第6期13卷 964-976页

作者： LI Yue QI Shi LI Jingjing College of Public administration Guizhou University of Finance and EconomicsGuiyang 550025China key laboratory of state forestry administration on Soil and Water Conservation Beijing Forestry UniversityBeijing 100083China

In the new era of the rapid development of economic globalization and the community of human destiny,the implementation of the “One Belt and One Road”(OBOR) construction model is designed to coordinate environmental protection and economic development. Most of the countries along the Silk Road in the 21st century are developing countries, and the majority of them are facing the same ecological and developmental difficulties as China. In this paper, under the background of the “OBOR” strategy and on the basis of the distribution of global climate types, we selected Central Asia and Northwest China, which have temperate continental climates, as the research objects. We sorted out and summarized the main ecological problems faced by Western China and Central Asia during the development of the “Belt and Road” initiative. At the same time, in combination with the major ecological governance projects implemented in recent years, we proposed key ecological governance technologies that have a certain degree of scalability, such as key technologies for water resource utilization and protection, sand prevention and control, and saline-alkali land governance. The aim was to offer the experiences and a reference for providing technological models for the “one belt along the road” region and the country to build an effective ecological governance system. Two suggestions are then proposed for improving the feasibility and rationality of ecological governance technology in the construction of the “One Belt, One Road”. 1) With the implementation of the strategy of “OBOR” construction, the ecological threats the OBOR countries are facing cannot be ignored. Every country needs to jointly act to build an “OBOR” ecological civilization. 2) The participants must pay attention to the spatial heterogeneity and temporal dynamics of ecological carrying capacity, and provide data reference and support for the reasonable allocation of ecological governance technology.

关键词： the“Belt and Road”initiative ecological civilization ecological governance ecological restoration technology

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我国小径桉树高值化利用研究进展

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林产工业 2013年第1期40卷 5-8页

作者：余养伦于文吉 Research Institute of Wood Industry Chinese Academy of Forestry key laboratory of Wood Science and Technology of state forestry administration

总结了我国桉树资源发展状况和工业化利用现状,介绍了高性能重组木、单板层积材、竹桉复合材料、厚芯实木复合板材和无醛胶合板制造技术最新研究进展,指出了小径级桉树木材高值化综合利用有效方法,并将桉树的综合利用率提高至90%以上,... 详细信息

总结了我国桉树资源发展状况和工业化利用现状,介绍了高性能重组木、单板层积材、竹桉复合材料、厚芯实木复合板材和无醛胶合板制造技术最新研究进展,指出了小径级桉树木材高值化综合利用有效方法,并将桉树的综合利用率提高至90%以上,生产过程耗能低,二氧化碳排放少,符合我国目前低碳经济发展的需要。

关键词：小径桉树高值化利用高性能重组木厚芯实木复合板材

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Two Zinc(Ⅱ) Complexes Based on 7rany-(1R,2R)-cyclohexanediamine: Molecular Structure Analyses and Preparation of Composite Membrane

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Chemical Research in Chinese Universities 2019年第5期35卷 749-754页

作者： WANG Dawei WANG Ying ZHUANG Changfu ZHAO Ping YANG Jing SHI Zhengjun key laboratory for Forest resources Conservation and utilization in the Southwest Mountains of China Ministry of EducationSouthwest Forestry UniversityKunming 650224P.R.China key L aboratory of state forestry administration on Highly-efficient utilization of forestry Biomass resources in Southwest China Southwest Forestry UniversityKunming 650224P.R.China

Two novel complexes Zn2(chda)2(TPA)Cl2(1) and [Zn(chda)2(H2O)2](TPAXH2O)2(2)[chda is a chiral ligand of trans-(1R,2R)-cyclohexanediamine, H2TPA is terephthalic acid] were synthesized at room temperature. The molecular structures of complexes 1 and 2 were identified by single-crystal X-ray diffraction measurement. The results showed that complex 1 had a binuclear structure, each Zn(Ⅱ) ion was four coordinated by two N atoms of chda, one O atom of TPA and one Cl atom, to construct a distorted tetrahedral geometry. For complex 2, the Zn(Ⅱ) ion was six coordinated by two chda molecules and two water molecules to form a ZnN4O2 octahedral geometry. Complexes 1 and 2 further extended into three-dimensional supramolecular frameworks by hydrogen-bonding interactions. Hirshfeld surface analysis and fingerprint plots were used to further analyze the contribution of diflerent intermolecular interactions. Further, a composite membrane [Zn(chda)2(H2O)2](TPA)(H2O)2/CA was prepared by adding 10%(mass fraction) of complex 2 into cellulose acetate using acetone/chloroform( V:V-1:1) as solvent.

关键词： Zinc(Ⅱ) complex Trans-(1R,2R)-cyclohexanediamine Hirshfeld surface analysis Composite membrane

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