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Morphological changes and allometric growth in hatcheryreared Chinese loach Paramisgurnus dabryanus(Dabry de Thiersant, 1872)

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Chinese Journal of Oceanology and Limnology 2016年第4期34卷 757-762页

作者：张云龙吴巧婉胡伟华汪帆邵韦涵张诚明赵忠波何辉樊启学顾泽茂 College of Fishery Huazhong Agricultural University Key Laboratory of Freshwater Animal Breeding Ministry of Agriculture

The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power model was estimated for the length-weight relationship during the early life stages. Positive allometric growth for the head segment, trunk length, tail length and eye diameter was also found in the early life stages, while body depth, tail depth, tail fi n length, pectoral fi n length and barbel length displayed a negative coeffi cient. During the subsequent early developmental stage, the growth coeffi cients showed a clear and common tendency towards isometry for all measured body ratios. The allometric growth changes in Chinese loach during the early stage are possibly the result of selective organogenesis directed towards survival priorities.

关键词： Paramisgurnus dabryanus early life stages length-weight relationship allometric growth

Molecular mapping of QTLs for root response to phosphorus deficiency at seedling stage in wheat (Triticum aestivum L.)

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Progress in Natural Science:Materials International 2007年第10期17卷 1177-1184页

作者： Li Zhenxing, Ni Zhongfu, Peng Huiru, Liu Zhiyong, Nie Xiuling, Xu Shengbao, Liu Gang and Sun Qixin(key laboratory of Crop Heterosis and Utilization and State key laboratory for Agrobiotechnology, key laboratory of Crop Genomics and Genetic Improvement, Beijing key laboratory of Crop Genetic Improvement, Department of Plant Genetics & breeding, China Agricultural University, Beijing 100094, China) key laboratory of Crop Heterosis and Utilization and State key laboratory for Agrobiotechnology Key Laboratory of Crop Genomics and Genetic Improvement Beijing Key Laboratory of Crop Genetic Improvement Department of Plant Genetics & Breeding China Agricultural University Beijing 100094 China

Phosphorus (P) deficiency in the soil is one of the major abiotic stresses that limit plant growth and crop productivity throughout the world. Development of cultivars with improved P-deficiency tolerance is an efficient strategy for sustainable agriculture. Plant roots play an important role in crop growth and development, especially in nutrient uptake and improvement of P-efficiency. Mapping quantitative trait loci (QTLs) for root traits and their response to low P stress at seedling stage will facilitate the development of P-efficient wheat cultivars. In this study, 30 QTLs (LOD>2.0) were mapped for the three root traits, such as root length, root number and root dry matter under different P supply conditions and their response to P-stress. These QTLs were distributed on 14 chromosomes, with each of the 5 QTLs explaining more than 10% phenotype variance. Analyses showed that root traits and their response to P-deficiency were controlled by different QTLs. In addition, alleles with positive effects were separated on both parents, and wheat cultivars with improved P-efficiency could be developed by accumulating these positive effect alleles together.

关键词： QTLs roots wheat seedling RIL P-deficiency.

Dissolution behavior of calcium-magnesium-silicate glass fiber

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Journal of Central South University 2011年第6期18卷 1833-1837页

作者：刘浩王玺堂张保国王周福 key State laboratory breeding Base of Refractories and Ceramics Wuhan University of Science and Technology

The dissolution behavior of CaO-MgO-SiO2 glass fiber was investigated by scanning electron microscopy （SEM）, Fourier-transform infrared spectrometer （FTIR） and inductively coupled plasma atomic emission spectroscopy （ICP-AES） using in-vitro tests. The results show that the soaked fiber is surrounded by an outer calcium-magnesium silicate hydrated layer, and there exists a balancing fimction between the formation and abscission of the hydrated layer during the dissolution process. The concentrations of leached ions increase constantly, and the mass loss of the fibers and pH changes of the solution are found to rise rapidly during the initial dissolution process, then their increasing rates are controlled by the balancing function of the hydrated layer at the subsequent dissolution stages. The dissolution rate constant and time for complete dissolution are estimated to be 274 ng/（cm2.h） and 15.2 d, respectively, presenting preferable biosolubilities.

关键词： calcium-magnesium-silicate glass glass fiber dissolution hydrated layer

The proteome of IVF-induced aberrant embryo-maternal crosstalk by implantation stage in ewes

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Journal of Animal Science and Biotechnology 2020年第2期11卷 393-409页

作者： Qianying Yang Wei Fu Yue Wang Kai Miao Haichao Zhao Rui Wang Min Guo Zhilong Wang Jianhui Tian Lei An key laboratory of Animal Genetics Breeding and Reproduction of the Ministry of AgricultureNational Engineering Laboratory for Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background:Implantation failure limits the success of in vitro fertilization and embryo transfer(IVF-ET).Wellorganized embryo-maternal crosstalk is essential for successful *** studies mainly focused on the aberrant development of in vitro fertilized(IVF)*** contrast,the mechanism of IVF-induced aberrant embryo-maternal crosstalk is not well ***:In the present study,using ewes as the model,we profiled the proteome that features aberrant IVF embryo-maternal crosstalk following *** comparing in vivo(IVO)and IVF conceptuses,as well as matched endometrial caruncular(C)and intercaruncular(IC)areas,we filtered out 207,295,and 403 differentially expressed proteins(DEPs)in each *** functional analysis showed that the IVF conceptuses were characterized by the increased abundance of energy metabolism and proliferation-related proteins,and the decreased abundance of methyl metabolism-related *** addition,IVF endometrial C areas showed the decreased abundance of endometrial remodeling and redox homeostasis-related proteins;while IC areas displayed the aberrant abundance of protein homeostasis and extracellular matrix(ECM)interaction-related *** on these observations,we propose a model depicting the disrupted embryo-maternal crosstalk following IVF-ET:Aberrant energy metabolism and redox homeostasis of IVF embryos,might lead to an aberrant endometrial response to conceptus-derived pregnancy signals,thus impairing maternal *** turn,the suboptimal uterine environment might stimulate a compensation effect of the IVF conceptuses,which was revealed as enhanced energy metabolism and ***:Systematic proteomic profiling provides insights to understand the mechanisms that underlie the aberrant IVF embryo-maternal *** might be helpful to develop practical strategies to prevent implantation failure following IVF-ET.

关键词： Conceptus Embryo-maternal crosstalk Endometrial C areas Endometrial IC areas Ewes HPLC-MS/MS IVF Proteome

Identification of an S5^n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

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Chinese Science Bulletin 2010年第13期55卷 1255-1262页

作者： SHAHID Muhammad Qasim Provincial key laboratory of Plant Molecular breeding South China Agricultural University

Oryza rufipogon exhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions of O. rufipogon were genetically examined using the functional marker S5n, revealing that 18 accessions carried the S5n allele with a heterozygous genotype (S5nS5i/j). Of these accessions, 14 were from Guangdong Province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan Province and 1 from Jiangxi Province. Further sequence analysis spanning both sides of the deletion in S5n allele of O. rufipogon confirmed that this allele is identical with that of 02428, a wide-compatibility variety of domesticated rice. The segregation ratio of the 3 genotypes (S5iS5i/S5jS5j:S5nS5i/j:S5nS5n) among 15 self-bred progenies of O. rufipogon significantly deviated from the Mendelian expectation (1:2:1) in that the proportion of plants exhibiting S5n heterozygous and homozygous genotypes was much lower in the progeny. Sequence analysis of full-length S5n alleles indicated that a few base pairs were different between O. rufipogon and O. sativa, and it was presumed that S5n may be an ancient allele. The fertility of the embryo sac in plants with an S5n heterozygous genotype was low, suggesting the existence of other genes controlling embryo sac fertility in the wild rice.

关键词：普通野生稻等位基因胚囊广西壮族自治区鉴定序列分析遗传多样性广亲和品种

Identification of QTLs for seed storability in rice under natural aging conditions using two RILs with the same parent Shennong 265

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Journal of Integrative Agriculture 2017年第5期16卷 1084-1092页

作者： DONG Xiao-yan FAN Shu-xiu LIU Jin WANG Qi LI Mei-rong JIANG Xin LIU Zhen-yu YIN Ye-chao WANG Jia-yu Rice Research Institute Shenyang Agricultural University/Key Laboratory of Northeast Rice Biology and BreedingMinistry of Agriculture/Key Laboratory of Northern japonica Rice Genetics and BreedingMinistry of EducationShenyang 110866P.R.China

Seed storability （SS） is an important trait for agronomic production and germplasm preservation in rice （Oryza sativa L.）. Quantitative trait locus （QTL） for seed storability in three storage periods was identified using two sets of recombinant inbred lines （RILs） derived from the crosses with a colnmon female parent Shennong 265 （SN265）. Ten QTLs for seed storability were detected on chromosomes 1, 2, 3, 4, 6, 8, and 12in SL-RILs （SN265/Lijiangxingtuanheigui （LTH））, and a total of 12 QTLs were identified on chromosomes 2, 3, 4, 6, 9, and 10 in SH-RILs （SN265/Luhui 99 （LH99））in different storage periods. Among these QTLs, five major QTLs were identified in more than one storage period. The qSS3-1, qSS3-2, qSS12-1, and qSS12-2 were detected in SL-RILs. Similarly, qSS2-2, qSS2-3, qSS6-2, qSS6-3, qSS6-4, qSS9-1, and qSS9-2 were detected in SH-RILs. In addition, the maximum phenotypic variation was derived from the qSS6-1 and qSS9-2, explaining 53.58 and 29.09%, respectively, while qSS6-1 was a new stable QTL for seed storability. These results provide an opportunity for pyramiding and map-based cloning major QTLs for seed storability in rice.

关键词： rice recombinant inbred lines natural aging seed storability quantitative trait locus

Mesoporous V-doped TiO_2 Nanoparticles as New Adsorbents for the Removal of Methylene Blue from Color Textile Wastewater

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Chinese Journal of Structural Chemistry 2015年第1期34卷 56-68页

作者：郑柳萍颜桂炀顾泉谢丽珊龙金林 College of Chemistry and Chemical Engineering Fujian Normal University Fujian key laboratory of Polymer Materials Department of Chemistry Ningde Normal University Fujian Provincial key laboratory of PhotocatalysisState key laboratory breeding Base

V-doped TiO2 nanoparticles（NPs） as dye adsorbents are synthesized by the co-precipitation method and characterized by X-ray powder diffraction, transmission electron microscope, N2 adsorption at 77 K, and X-ray photoelectron spectroscopy. The adsorption of methylene blue（MB） on the V-doped TiO2 NPs is studied in detail by varying the calcination temperature and V doping amount of the adsorbent, adsorbate concentration, adsorbent dosage, agitation rate, reaction temperature, and p H. The comparison of dye adsorption on V-doped TiO2 and parent TiO2 demonstrates that the adsorptive activity of TiO2 can be improved by V doping. The enhanced adsorptive performance can be attributed to the tremendous changes in texture, structure, and surface morphology of adsorbent. The adsorption kinetic analysis shows that the adsorption follows the pseudo-second order kinetics. The apparent activation energy for adsorption is calculated by Arrhenius formula to be 37.6 k J·mol-1, indicating that the adsorption is controlled by both of the diffusion and interfacial adsorption steps. The adsorption data are analyzed using Langmuir and Freundlich isotherms and the results indicate that the Langmuir model provides better correlation of the experimental data. The results conclusively show that the adsorption of MB is a spontaneous behavior and endothermic reaction with the ΔH value of 17.60 k J·mol-1.

关键词： V-doped TiO2, methylene blue, adsorption, kinetics, thermodynamics

Dietary iron regulates intestinal goblet cell function and alleviates Salmonella typhimurium invasion in mice

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Science China(Life Sciences) 2023年第9期66卷 2006-2019页

作者： Shuan Liu Zhenlin Dong Wenjie Tang Jian Zhou Liu Guo Chengyan Gong Guang Liu Dan Wan Yulong Yin laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of Sciences Beijing 101408China Animal breeding and Genetics key laboratory of Sichuan Province Livestock and Poultry Biological Products Key Laboratory of Sichuan ProvinceSichuan Animal Science AcademyChengdu 610066China

Iron is an important micronutrient that plays a vital role in host defenses and bacterial pathogenicity. As iron treatments increase the risk of infection by stimulating the growth and virulence of bacterial pathogens, their roles in anti-infection immunity have frequently been underestimated. To estimate whether adequate dietary iron intake would help defend against pathogenic bacterial infection, mice were fed iron-deficient(2 mg kg-1feed), iron-sufficient(35 mg kg-1feed), or iron-enriched diet(350 mg kg-1feed) for 12 weeks, followed by oral infection with Salmonella typhimurium. Our results revealed that dietary iron intake improved mucus layer function and decelerated the invasion of the pathogenic bacteria, Salmonella typhimurium. Positive correlations between serum iron and the number of goblet cells and mucin2 were found in response to total iron intake in *** iron in the intestinal tract affected the gut microbiota composition, and the abundance of Bacteroidales, family Muribaculaceae, was positively correlated with their mucin2 expression. However, the results from antibiotic-treated mice showed that the dietary iron-regulated mucin layer function was not microbial-dependent. Furthermore, in vitro studies revealed that ferric citrate directly induced mucin2 expression and promoted the proliferation of goblet cells in both ileal and colonic organoids. Thus, dietary iron intake improves serum iron levels, regulates goblet cell regeneration and mucin layer function, and plays a positive role in the prevention of pathogenic bacteria.

关键词： nutritional iron goblet cell gut microbiota mucin2

Advances and prospects of super rice breeding in China

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Journal of Integrative Agriculture 2017年第5期16卷 984-991页

作者： TANG Liang XU Zheng-jin CHEN Wen-fu Rice Research Institute Shenyang Agricultural University/Key Laboratory of Northeast Rice Biology and BreedingMinistry of Agriculture/Key Laboratory of Northern japonica Super Rice BreedingMinistry of EducationShenyang 110866P.R.China

Super rice breeding in China has been very successful over the past 3 decades, and the Chinese government has made great efforts to support breeding and cultivation of both conventional and hybrid super rice. In this review, we focus on the progress in and potential of super rice breeding. After the establishment of the breeding theory and strategy of ＂generating an ideotype with strong heterosis through inter-subspecies hybridization, by using gene pyramiding to combine elite traits through composite-crossing to breed super rice varieties with both ideotype and strong hybrid vigor＂, a series of major breakthroughs have been achieved in both conventional and super hybrid rice breeding. A number of new genetic materials with ideotype have been created successfully, and the Ministry of Agriculture of China has approved 156 novel super rice varieties and combinations for commercialization. During the Developing the Super Rice Varieties Program, great attention has also been paid to the integration and demonstration of the rice production technology. Collaboration between industry and university researchers has led to technological innovations and initiation of a demonstration system for super hybrid rice. With widespread cultivation of super rice with higher quality and yield, as well as resistance or tolerance to abiotic or biotic stresses, the yield of rice production per unit has reached a new level. In addition to increased quality and yield, hybrid rice breeding has also led to improvements in many other agronomic traits, such as resistance to pests and diseases, resistance to lodging, and optimized light distribution in population. Achievements in super rice breeding and innovation in rice production have made major contributions to the progress in rice sciences and worldwide food security.

关键词： super rice super hybrid rice ideotype heterosis breeding