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A Preliminary Study of Mapping Genes Underlying Complex Traits Based on Chromosome Segment Substitution Lines

分子植物育种 2007年第2期5卷 242-244页

作者： Z.X. Tang 　C.W. Xu jiangsu Provincial key laboratory of crop genetics and physiology Yangzhou University Yangzhou 225009 China

Complex traits are the features whose properties are determined by multiple factors, which can be genetic or environmental. Most of economically important characteristics of plants and animals belong to this special ... 详细信息

关键词：染色体复合体基因表达植物研究

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Current achievements and future prospects in the genetic breeding of chrysanthemum:a review

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Horticulture Research 2019年第1期6卷 378-396页

作者： Jiangshuo Su Jiafu Jiang Fei Zhang Ye Liu Lian Ding Sumei Chen Fadi Chen State key laboratory of crop genetics and Germplasm Enhancement Key Laboratory of LandscapingMinistry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural University210095 NanjingChina

Chrysanthemum(Chrysanthemum morifolium Ramat.)is a leading flower with applied value *** new chrysanthemum cultivars with novel characteristics such as new flower colors and shapes,plant architectures,flowering times,postharvest quality,and biotic and abiotic stress tolerance in a time-and cost-efficient manner is the ultimate goal for *** breeding strategies have been employed to improve the aforementioned traits,ranging from conventional techniques,including crossbreeding and mutation breeding,to a series of molecular breeding methods,including transgenic technology,genome editing,and marker-assisted selection(MAS).In addition,the recent extensive advances in high-throughput technologies,especially genomics,transcriptomics,proteomics,metabolomics,and microbiomics,which are collectively referred to as omics platforms,have led to the collection of substantial amounts of *** of these omics data with phenotypic information will enable the identification of genes/pathways responsible for important *** attempts have been made to use emerging molecular and omics methods with the aim of accelerating the breeding of ***,applying the findings of such studies to practical chrysanthemum breeding remains a considerable challenge,primarily due to the high heterozygosity and polyploidy of the *** review summarizes the recent achievements in conventional and modern molecular breeding methods and emerging omics technologies and discusses their future applications for improving the agronomic and horticultural characteristics of chrysanthemum.

关键词： breeding chrysanthemum cultivar

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An in vivo Transient Expression System Can Be Applied for Rapid and Effective Selection of Artificial MicroRNA Constructs for plant Stable Genetic Transformation

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Journal of genetics and genomics 2013年第5期40卷 261-270页

作者： Basdeo Bhagwat Ming Chi Li Su Haifeng Tang Guiliang Tang Yu Xiang Agriculture and Agri-Food Canada Pacific Agri-Food Research CenterSummerlandBritish Columbia VOH 1ZOCanada College of Forestry Northwest A & F University College of Horticulture Northwest A & F University key laboratory of physiology Ecologyand Genetic Improvement of Food Crop in Henan ProvinceCollege of AgricultureHenan Agricultural University Department of plant and Soil Sciences and KTRDC University of KentuckyLexingtonKY 40546 USA Department of plant and Soil Sciences and KTRDC University of KentuckyLexingtonKY 40546USA Department of Biological Sciences Michigan Technological UniversityHoughtonMI 49931USA

The utility of artificial microRNAs（amiRNAs） to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable,In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the *** genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of *** provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.

关键词： Artificial microRNA Agrobacterium-mediated transient expression plant genetic transformation

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高游离赖氨酸转基因水稻在SD大鼠中的亚慢毒性研究

高游离赖氨酸转基因水稻在SD大鼠中的亚慢毒性研究

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作者： Qing-Qing Yang Xiao-Yun He Hong-Yu Wu Chang-Quan Zhang Shi-Ying Zou Tian-Qi Lang Samuel Sai-Ming Sun Qiao-Quan Liu 胡贻椿 key laboratory of crop genetics and physiology of jiangsu province/key laboratory of plant functional genomics of the ministry of education College of AgricultureYangzhou University State key laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong Kong Beijing Advanced Innovation Center for Food Nutrition and Human Health College of Food Science and Nutritional EngineeringChina Agricultural University Co-Innovation Center for Modern Production Technology of Grain crops of jiangsu province/Joint International Research laboratory of Agriculture and Agri-Product Safety of the ministry of education Yangzhou University

赖氨酸被认为是水稻中的第一个必需氨基酸。前期研究中采用代谢工程法调控赖氨酸代谢培育了高游离赖氨酸转基因品系HFL1。在本研究中,我们采用不同的饲喂剂量对该品系进行了SD大鼠90天毒性试验,从而研究HFL1大米对SD大鼠的潜在健康效应... 详细信息

赖氨酸被认为是水稻中的第一个必需氨基酸。前期研究中采用代谢工程法调控赖氨酸代谢培育了高游离赖氨酸转基因品系HFL1。在本研究中,我们采用不同的饲喂剂量对该品系进行了SD大鼠90天毒性试验,从而研究HFL1大米对SD大鼠的潜在健康效应。试验过程中记录体重增加、进食量、食物利用率等。中期和末期测定血液学、血化学指标,试验结束时记录脏器重量和进行组织病理学检测。结果表明,与非转基因组(N)或对照组相比,转基因(T)大米饲料对大鼠无不良影响。饲喂T组与饲喂N组或对照饲料的大鼠之间,大多数血液学、血化学指标和脏器系数都没有显著差异。在T组与相应的N组和/或对照组之间有个别指标具有差异,但均没有观察到不良的组织病理效应。综上所述,本研究的结果表明在90天内给予含有70%高赖氨酸大米HFL1转基因饲料后,不会对SD大鼠产生不良影响。

关键词：转基因水稻高游离赖氨酸饲喂研究毒理学大鼠

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Proximal and remote sensing in plant phenomics: 20 years of progress, challenges, and perspectives

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plant Communications 2022年第6期3卷 135-173页

作者： Haiyu Tao Shan Xu Yongchao Tian Zhaofeng Li Yan Ge Jiaoping Zhang Yu Wang Guodong Zhou Xiong Deng Ze Zhang Yanfeng Ding Dong Jiang Qinghua Guo Shichao Jin plant Phenomics Research Centre Academy for Advanced Interdisciplinary StudiesNational Engineering and Technology Center for Information AgricultureCollaborative Innovation Centre for Modern Crop Production co-sponsored by Province and MinistryNanjing Agricultural UniversityAddress:No.1 WeigangXuanwu DistrictNanjing 210095China The key laboratory of Oasis Eco-agriculture Xinjiang Production and Construction CorpsAgriculture CollegeShihezi UniversityShihezi 832003China Hainan Yazhou Bay Seed laboratory Sanya 572025China State key laboratory of crop genetics and Germplasm Enhancement National Center for Soybean ImprovementKey Laboratory for Biology and Genetic Improvement of Soybean(GeneralMinistry of Agriculture)Nanjing Agricultural UniversityNanjing 210095China Sanya Research Institute of Nanjing Agriculture University Sanya 572024China key laboratory of plant Molecular physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Institute of Ecology College of Urban and Environmental SciencePeking UniversityBeijing 100871China jiangsu Provincial key laboratory of Geographic Information Science and Technology International Institute for Earth System SciencesNanjing UniversityNanjingJiangsu 210023China

plant phenomics(PP)has been recognized as a bottleneck in studying the interactions of genomics and environment on plants,limiting the progress of smart breeding and precise ***-throughput plant phenotyping is challenging owing to the spatio-temporal dynamics of *** and remote sensing(PRS)techniques are increasingly used for plant phenotyping because of their advantages in multi-dimensional data acquisition and *** progress of PRS applications in PP has been observed over the last two decades and is analyzed here from an interdisciplinary perspective based on 2972 *** progress covers most aspects of PRS application in PP,including patterns of global spatial distribution and temporal dynamics,specific PRS technologies,phenotypic research fields,working environments,species,and ***,we demonstrate how to link PRS to multi-omics studies,including how to achieve multi-dimensional PRS data acquisition and processing,how to systematically integrate all kinds of phenotypic information and derive phenotypic knowledge with biological significance,and how to link PP to multi-omics association ***,we identify three future perspectives for PRS-based PP:(1)strengthening the spatial and temporal consistency of PRS data,(2)exploring novel phenotypic traits,and(3)facilitating multi-omics communication.

关键词： plant phenomics remote sensing phenotyping phenotypic traits multi-omics breeding precision cultivation

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Calcium Addition Affects Germination and Early Seedling Growth of Sweet Sorghum under Saline Conditions

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Agricultural Science & Technology 2012年第12期13卷 2538-2543页

作者： Guisheng ZHOU B.L.MA key Lab of crop genetics & physiology of jiangsu province Yangzhou University Eastern Cereal and Oilseed Research Center(ECORC) Agriculture and Agri-Food CanadaCentral Experimental Farm960 Carling Ave.OttawaONCanadaK1A 0C6

[Objective] This study aimed to determine the interactive effects of supple- mental Ca amendment and salinity on germination of sweet sorghum seeds in saline solution culture medium, and investigate the effects of different combinations of Na/Ca ratio in saline soils on the early growth of sweet sorghum plants. [Method] A germi- nation test and a greenhouse pot experiment were conducted to assess the interac- tive effects of calcium addition to culture medium on the germination and seedling growth of sweet sorghum （Sorghum saccharatum Moench） in saline soils with a range of NaYCa ratios. In the germination test, seeds were treated with different combinations of five calcium levels [0, 5, 10, 15, and 20 mmol/L Ca（NO3）2] and five salinity levels （0, 50, 100, 150, and 200 mmol/L NaCI）. In the greenhouse experi- ment, seeds were sown in potting soils containing 3 salinity levels （2.3, 4.7 and 7.0 dS/m） and three Na：Ca ratios （10：0, 10：1, and 5：1）. [Result] In the germination test, Ca addition at 5 mmol/L promoted germination by 5.5%, 9.9%, and 17.0% at the 3.4, 6.7 and 10.1 dS/m salinity levels. The higher Ca level （10 mmol/L） also in- creased germination by 9.1% and 7.8% at the 3.4 and 6.7 dS/m salinity levels. Then even higher Ca addition at 15 and 20 mmol/L appeared to promote germina- tion when culture media had high salinity （10.1 and 13.4 dS/m）. In the greenhouse pot experiment, saline soil amended with supplemental Ca at the 2.3 and 4.7 dS/m salinity levels significantly promoted early seedling growth, with an increase of 6.8% to 28.2% in plant height and 14.3% to 67.9% in whole plant weight. From 28 to 42 d after seeding, the relative growth of seedling was increased by Ca addition, with a reduction of 49.5% to 66.0% in plant height and 4.8% to 61.9% in whole plant weight. From 42 to 56 d after seeding, however, the relative growth of seedling was significantly inhibited by Ca amendment. [Conclusion] Results of this study indicate that appropriate supplemental C

关键词： Sweet sorghum Salinity Calcium

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