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Journal of Integrative plant Biology 2015年第8期57卷 688-697页

作者： Liqing Gu Bo Wei Renchun Fan Xu Jia Xianping Wang Xiangqi Zhang State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences

chromosome segmental introgression lines（ILs）are an effective way to utilize germplasm resources in crops.To improve agronomic traits of wheat cultivar（Triticum aestivum） Shi 4185, four sets of ILs were developed. The donors were Chinese endemic subspecies accessions Yunnan wheat（T. aestivum ssp. yunnanense） YN3, Tibetan semiwild wheat（T. aestivum ssp. tibetanum） XZ-ZM19450, and Xinjiang wheat（T. aestivum ssp. petropavlovskyi） XJ5, and synthetic wheat HC-XM1620 derived from a cross between T. durum acc. D67.2/P66.270 with Aegilops tauschii acc. 218.Totals of 356, 366, 445 and 457 simple sequence repeat（SSR）markers were polymorphic between Shi 4185 and YN3, XZZM19450, XJ5 and HC-XM1620, respectively. In total, 991 ILs were identified, including 300 derived from YN3, covering 95%of the genome of Shi 4185, 218 from XZ-ZM19450（79%）, 279 from XJ5（97%）, and 194 from HC-ZX1620（84%）. The sizes and locations of each introgression were determined from a consensus SSR linkage map. Using the ILs, 11 putative Resear quantitative trait loci（QTLs） were identified for plant height（PH）, spike length（SL） and grain number per spike（GNS）.Comparative analyses of 24 elite ILs with the parents revealed that the four donor parents could be important resources to improve wheat SL and GNS. Our work offers a case for utilizing endemic landraces for QTL mapping and improvement of wheat cultivars using introgression lines.

关键词： chromosome segmental introgression lines commonwheat molecular breeding quantitative trait locus simple sequencerepeat marker

Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene

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Journal of Genetics and Genomics 2017年第9期44卷 465-468页

作者： Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling Chen Jinxing Liu Huabang Chen Caixia Gao State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences University of Chinese Academy of Sciences State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Maize（Zea mays L.）is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013（Baldaufa et al.,2016）.Heterosis is widely used to increase the productivity of maize,and the first ... 详细信息

关键词： PCR RE Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene

Genomics progress will facilitate molecular breeding in soybean

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中国科学：生命科学英文版 2015年第8期58卷 813-815页

作者： WANG Zheng TIAN ZhiXi State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences

It has been suggested that the soybean(Glycine max[L.]Merr.)currently cultivated in commercial agriculture was domesticated from the wild soybean(G.soja Sieb.&Zucc.)in China approximately 5,000 years ago.Because of its high protein and oil content,cultivated soybean has become a major economic crop by providing 69%and 30%of the world’s plant protein and oil.Soybean breeders have

关键词：野生大豆基因组学分子育种植物蛋白遗传基础农艺性状基因组序列栽培驯化

Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

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Journal of Genetics and Genomics 2014年第2期41卷 63-68页

作者： Zhen Liang Kang Zhang Kunling Chen Caixia Gao State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Transcription activator-like effector nucleases （TALENs） and clustered regularly interspaced short palindromic repeats （CRISPR）/ CRISPR-associated （Cas） systems have emerged as powerful tools for genome editing in a variety of species. Here, we report, for the first time, targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. We designed five TALENs targeting 4 genes, namely ZmPDS, ZmlPKIA, ZmlPK, ZmMRP4, and obtained targeting efficiencies of up to 23.1% in protoplasts, and about 13.3% to 39.1% of the transgenic plants were somatic mutations. Also, we constructed two gRNAs targeting the ZmlPK gene in maize protoplasts, at frequencies of 16.4% and 19.1%, respectively. In addition, the CRISPR/Cas system induced targeted mutations in Z. mays protoplasts with efficiencies （13.1%） similar to those obtained with TALENs （9.1%）. Our results show that both TALENs and the CRISPR/Cas system can be used for genome modification in maize.

关键词： TAL-effector nucleases CRISPR/Cas system Knock-out Zea mays

TALENs:Customizable Molecular DNA Scissors for Genome engineering of plants

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Journal of Genetics and Genomics 2013年第6期40卷 271-279页

作者： Kunling Chen Caixia Gao State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Precise genome modification with engineered nucleases is a powerful tool for studying basic biology and applied biotechnology. Transcription activator-like effector nucleases（TALENs）,consisting of an engineered specific（TALE） DNA binding domain and a Fok I cleavage domain,are newly developed versatile reagents for genome engineering in different organisms.Because of the simplicity of the DNA recognition code and their modular assembly,TALENs can act as customizable molecular DNA scissors inducing double-strand breaks（DSBs） at given genomic location.Thus,they provide a valuable approach to targeted genome modifications such as mutations, insertions,replacements or chromosome rearrangements.In this article,we review the development of TALENs,and summarize the principles and tools for TALEN-mediated gene targeting in plant cells,as well as current and potential strategies for use in plant research and crop improvement.

关键词： TALENs Genome engineering Targeted gene modification plant

Sequence Diversity and Enzyme Activity of Ferric-Chelate Reductase LeFRO1 in Tomato

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Journal of Genetics and Genomics 2013年第11期40卷 565-573页

作者： Danyu Kong Chunlin Chen Huilan Wu Ye Li Junming Li Hong-Qing Ling State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Ferric-chelate reductase which functions in the reduction of ferric to ferrous iron on root surface is a critical protein for iron ho- meostasis in strategy I plants. LeFROI is a major ferric-chelate reductase involved in iron uptake in tomato. To identify the natural variations of LeFRO1 and to assess their effect on the ferric-chelate reductase activity, we cloned the coding sequences of LeFRO1 from 16 tomato varieties collected from different regions, and detected three types of LeFRO1 （LeFRO1MM, LeFRO1Ailsa and LeFRO1Monita） with five amino acid variations at the positions 21, 24, 112, 195 and 582. Enzyme activity assay revealed that the three types of LeFRO1 possessed different ferric-chelate reductase activity （LeFRO1AiISa 〉 LeFRO1MM 〉 LeFRO1M^nita）. The 112th amino acid residue Ala of LeFRO1 is critical for maintaining the high activity of ferric-chelate reductase, because modification of this amino acid resulted in a significant reduction of enzyme activity. Further, we showed that the combination of the amino acid residue lie at the site 24 with Lys at the site 582 played a positive role in the enzyme activity of LeFRO1. In conclusion, the findings are helpful to understand the natural adaptation mechanisms of plants to iron-limiting stress, and may provide new knowledge to select and manipulate LeFRO1 for improving the iron deficiency tolerance in tomato.

关键词： Tomato Iron homeostasis Natural variation LeFRO1 Ferric-chelate reductase activity

New insights into plant nutrient signaling and adaptation to fluctuating environments

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Journal of Genetics and Genomics 2016年第11期43卷 621-622页

作者： Xiangdong Fu The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Over the past 50 years, the Green Revolution and exploitation of heterosis have allowed cereal grain yield to keep pace with world- wide population growth. Unfortunately, plant growth and crop productivity are heavily... 详细信息

关键词： AMT New insights into plant nutrient signaling and adaptation to fluctuating environments PUE Pi

Heterotrimeric G proteinγsubunit DEP1 synergistically regulates grain quality and yield by modulating the TTP(TON1-TRM-PP2A)complex in rice

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Journal of Genetics and Genomics 2023年第7期50卷 528-531页

作者： Yunzhe Wu Ying Zhao Jianping Yu Chenchen Wu Qi Wang Xueying Liu Xiuhua Gao Kun Wu Xiangdong Fu Qian Liu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Shandong University of Traditional Chinese Medicine JinanShandong 250355China College of plant Science and Technology Key Laboratory of New Technology in Agricultural ApplicationBeijing University of AgricultureBeijing 102206China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesCollege of Life SciencesUniversity of Chinese Academy of SciencesBeijing 100101China

Rice is the staple food for nearly half of the world’s population.The negative correlation between rice yield and quality confers breeders a major challenge in how to improve these two traits synergistically.The grain shape,including grain length,grain width,and the ratio of grain length to width,plays an important role in determining rice yield potential and end-use quality.Thus far,some genes determining grain shape and appearance quality,such as GRAIN SIZE 3(GS3)(Fan et al.,2006),GRAIN WIDTH AND WEIGHT 5(GW5)(Liu et al.,2017),GRAIN WIDTH 8(GW8)(Wang et al.,2012),GRAIN SIZE 5(GS5)(Li et al.,2011),GRAIN LENGTH 7/GRAIN WIDTH 7(GL7/GW7)(Wang et al.,2015a,2015b),GRAIN LENGTH AND WEIGHT(GLW7)(Si et al.,2016),GRAIN LENGTH 4(GL4)(Wu et al.,2017),LONG GRAIN AND HIGH YIELD 3(LGY3)(Liu et al.,2018),GRAIN SIZE 9(GS9)(Zhao et al.,2018),GRAIN LENGTH 10(GL10)(Zhan et al.,2022),and BASIC LEUCINE ZIPPER 60(Osb-ZIP60)(Yang et al.,2022),have been cloned and characterized.However,only a few genes have been demonstrated to simultaneously improve grain yield and quality,and the underlying genetic interaction and network also remain largely unknown.

关键词： grain synergistic quality

Alteration of Terminal Heterochromatin and chromosome Rearrangements in Derivatives of Wheat-Rye Hybrids

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Journal of Genetics and Genomics 2013年第8期40卷 413-420页

作者： Shulan Fu Zhenling Lv Xiang Guo Xiangqi Zhang Fangpu Han State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Geneticsand Developmental BiologyChinese Academy of Sciences key laboratory of plant Breeding and Genetics Sichuan Agriculture University

Wheat-rye addition and substitution lines and their self progenies revealed variations in telomeric heterochromatin and centromeres, Furthermore, a mitotically unstable dicentric chromosome and stable multicentric chromosomes were observed in the progeny of a Chinese Spring-lmperial rye 3R addition line. An unstable multicentric chromosome was found in the progeny of a 6R/6D substitution line. Drastic variation of terminal heterochromatin including movement and disappearance of terminal heterochromatin occurred in the progeny of wheat- rye addition line 3R, and the 5RS ditelosomic addition line. Highly stable minichromosomes were observed in the progeny ofa monosomic 4R addition line, a ditelosomic 5RS addition line and a 6R/6D substitution line. Minichromosomes, with and without the FISH signals for telomeric DNA （TTTAGGG）n, derived from a monosomic 4R addition line are stable and transmissible to the next generation. The results indicated that centromeres and terminal heterochromatin can be profoundly altered in wheat-rye hybrid derivatives.

关键词： Wheat-Rye addition lines chromosome rearrangements Multiple centromeres Minichromosomes Heterochromatin

Super graph-based pan-genome: Bringing rice functional genomic study into a new dawn

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Molecular plant 2022年第9期15卷 1409-1411页

作者： Yucheng Liu Zhixi Tian State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100039China

Rice is one of the earliest domesticated crops that has been cultivated for thousands of years.Interestingly,unlike other crops that are predominantly domesticated by a singleto neventin one place,the domestication of rice is quite complicated;it independently occurred in Asia and Africa toform the Asian cultivated rice Oryza sativa and the African cultivated rice O.glaberrima,respectively.O.sativa was mainly domesticated from the Asian wild rice O.rufipogon in East/South Asia(Huang et al.,2012).Furthermore,O.sativa can be classified into two main types:Geng(O.sativa ssp.japonica)and Xian(O.sativa ssp.indica).Geng was domesticated as early as 9000 years ago,while Xian was domesticated lateralong with continuousgene flow from Geng(Choi etal.,2017).O.glaberrimawas domesticated from African wild rice O.barthi approximately 3500 years ago.O.barthii and O.rufipogon diverged approximately 600000 years ago.

关键词： cultivated crops japonica