检索结果-南通市图书馆

Structural and functional analysis of an asymmetric bidirectional promoter in Arabidopsis thaliana

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2015年第2期57卷 162-170页

作者： Shi-Juan Liu Qiu-Juan Yue Wei Zhang key laboratory of plant cell engineering and germplasm innovation Ministry of Education School of Life Sciences Shandong University College of Life Science Qufu Normal University

Bidirectional promoters are relatively abundant in eukaryotic genomes, suggesting that they have an important biological significance. As yet, few of these promoters have been characterized in detail. Here, using a promoter：：GUS transgene approach has revealed that the intergenic region of Arabidopsis thaliana divergent genes At1g71850 and At1g71860 is an asymmetric bidirectional promoter, which exhibits an orientation-dependent expression profile. The strength of the forward promoter was greater than that of the reverse promoter, and their tissue specificities were not identical. Deletion analyses revealed that this bidirectional promoter could be divided into three functional regions. The basal level and tissue specificity of the promoter in the reverse orientation were regulated positively by region II and negatively by region III, whereas promoter activity in the forward orientation was regulated negatively by region II and positively by region I. Thus the 52-bp stretch of region II had a dual function, enhancing expression in the reverse orientation and suppressing it in the forward orientation. These results demonstrated that the activity of the At1g71850-At1g71860 bidirectional promoter was modulated by complex interactions between both positive and negative cis-acting elements. These findings will enhance our understanding of the regulatory mechanisms of plant bidirectional promoters.

关键词： GUS (β-glucuronidase) positive and negative regulatoryelement promoter strength tissue-specific expression

The landscape of molecular mechanisms for salt tolerance in wheat

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2018年第1期6卷 42-47页

作者： Meng Wang Guangmin Xia The key laboratory of plant cell engineering and germplasm innovation Ministry of Education School of Life Sciences Shandong University State key laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences

Wheat is one of the most important food crops, and its yield is seriously restricted by high salinity and other abiotic stresses. Many attempts have been made to elucidate the major physiological processes associated with salt tolerance and to identify the genes controlling the processes. In this review, the major role of high-affinity potassium transporter(HKT)genes in enhancing the salt tolerance of wheat is summarized. The link between maintenance of reactive oxygen species(ROS) homeostasis and salt tolerance through a comprehensive study of a wheat introgression line is examined, and the contribution of a set of genes involved in this process is depicted. New research strategies to uncover the mechanisms underlying salt tolerance in wheat based on recent advances in omics will be discussed.

关键词： Ionic and ROS homeostasis Molecular breeding Salt tolerance Triticum aestivum

ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHE Lexpression

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2017年第10期59卷 747-758页

作者： Xuehuan Dai Zhenhua Liu Meng Qiao Juan Li Shuo Li Fengning Xiang The key laboratory of plant cell engineering and germplasm innovation School of Life Sciences Shandong University Jinan 250100 China Shandong Province Administration of Work Safety Jinan 250100 China

Auxin and cytokinin direct cell proliferation and differentiation during the in vitro culture of plant cells, but the molecular basis of these processes, especially de novo shoot regeneration, has not been fully elucidated. Here, we describe the regulatory control of shoot regeneration in Arabidopsis thaliana(L.) Heynh, based on the interaction of ARABIDOPSIS RESPONSE REGULATOR12(ARR12) and WUSCHEL(WUS). The major site of ARR12 expression coincided with the location where the shoot apical meristem(SAM) initiated. The oar12 mutants showed severely impaired shoot regeneration and reduced responsiveness to cytokinin; consistent with this, the overexpression of ARR12 enhanced shoot *** shoot meristem specification genes, notably WUSCHEL(WUS) and CLAVATA3, were significantly downregulated in the arr1z explants. Chromatin immunoprecipitation(ChIP) and transient activation assays demonstrated that ARR12 binds to the promoter of WUS. These observations indicate that during shoot regeneration, in vitro, ARR12 functions as a molecular link between cytokinin signaling and the expression of shoot meristem specification genes.

关键词： ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHEL expression Figure

EZH2-dependent myelination following sciatic nerve injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2382-2394页

作者： Hui Zhu Li Mu Xi Xu Tianyi Huang Ying Wang Siyuan Xu Yiting Wang Wencong Wang Zhiping Wang Hongkui Wang Chengbin Xue Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityKey Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Department of Neurology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Critical Care Medicine Nantong Fourth People’s HospitalNantongJiangsu ProvinceChina

Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve ***,the gene regulatory network of regenerated myelin differs from that of native *** of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in *** further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were *** results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain *** highlights the crucial role of Ezh2 in initiating Schwann cell ***,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type *** suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and *** conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve *** EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.

关键词： demyelination EZH2 myelination peripheral nerve injury PRC2 remyelination Schwann cells sciatic nerve crush sciatic nerve transection

rhe Mitochondrion-Targeted PENTATRICOPEPTIDE REPEAT78 Protein Is Required for nad5 Mature mRNA Stability and Development in Maize

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2017年第10期10卷 1321-1333页

作者： Ya-Feng Zhang Masaharu Suzuki Feng Sun Bao-Cai Tan key laboratory of plant cell engineering and germplasm innovation Ministry of Education School of Life Sciences Shandong University Jinan 250100 China Guangdong Provincial key laboratory of plant Molecular Breeding College of Agriculture South China Agricultural University Guangzhou 510642 China plant Molecular and cellular Biology Program University of Florida Gainesville Florida 32611 USA

Pentatricopepetide repeat （PPR） proteins are a large family of RNA-binding proteins involved in RNA meta- bolism in plant organelles. Although many PPR proteins have been functionally studied, few of them are identified with a function in mitochondrial RNA stability. By using a reverse genetic approach, we characterized the role of the mitochondrion-targeted PPR78 protein in nad5 mature mRNA stability and maize （Zea mays） seed development. Loss of PPR78 function leads to a dramatic reduction in the steady-state level of mitochondrial nad5 mature mRNA, blocks the assembly of complex I in the electron transport chain, and causes an arrest in embryogenesis and endosperm development. Characterization of a second strong allele confirms the function of PPR78 in nad5 mRNA accumulation and maize seed development. The generation of mature nad5 requires the assembly of three distinct precursor RNAs via transsplicing reactions, and the accumulation ofnad5T1 precursor is reduced in the ppr78 mutants. However, it is the instability of mature nad5 rather than nad5T1 causing loss of the full-length nad5 transcript, and degradation of nad5 losing both translation start and stop codons is enriched in the mutant. Our data imply the assembly of mature nad5 mRNA precedes the protection of PPR78.

关键词： PPR nad5 mitochondrial RNA stability complex I seed development maize

Genome-wide association analysis identified molecular markers and candidate genes for flower traits in Chinese orchid (Cymbidium sinense)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2023年第11期10卷 193-207页

作者： Fengxi Yang Yudi Guo Jie Li Chuqiao Lu Yonglu Wei Jie Gao Qi Xie Jianpeng Jin Genfa Zhu Guangdong key laboratory of Ornamental plant germplasm innovation and Utilization Environmental Horticulture Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China

The orchid,the champagne of f lowers,brings luxury,elegance,and novelty to *** sinense is a symbol of gigantic floral variability on account ofwavering shapes and sizes of f loral organs,althoughmarker-trait association(MTA)has not been studied for its f loral *** evaluated markers associated with 14 f loral traits of *** through a genome-wide association study(GWAS)of 195 accessions.A total of 65318522 single-nucleotide polymorphisms(SNPs)and 3906176 insertion/deletion(InDel)events were identified through *** these,4694 potential SNPs and 477 InDelswere identified as MTAs at−log10 P>*** genes related to these SNPs and InDels were largely associated with f loral regulators,hormonal pathways,cell division,and metabolism,playing essential roles in tailoring f loral ***,20 candidate SNPs/InDels linked to 11 genes were verified,8 of which were situated on exons,onewas located in the 5-UTR and twowere positioned in ***,themultitepal trait-related gene RABBIT EARS(RBE)was found to be the most crucial *** analyzed the role of CsRBE in the regulation of flower-related genes via efficient transient overexpression in *** protoplasts,and found that the floral homeotic genes CsAP3 and CsPI,as well as organ boundary regulators,including CsCUC and CsTCP genes,were regulated by ***,we obtained key gene loci for important ornamental traits of orchids using genome-wide association analysis of populations with natural *** findings of this study can do a great deal to expedite orchid breeding programs for shape variability.

关键词： traits analysis breeding

SlGH9-15 regulates tomato fruit cracking with hormonal and abiotic stress responsiveness cis-elements

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第2期22卷 447-463页

作者： LIN Hao-wei WU Zhen ZHOU Rong CHEN Bin ZHONG Zhao-jiang JIANG Fang-ling College of Horticulture Nanjing Agricultural University/Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East ChinaMinistry of AgricultureNanjing 210095P.R.China

Fruit cracking occurs easily during the late period of fruit development when plants encounter an unsuitable environment,dramatically affecting fruit production and *** study conducted the bulked segregant RNA-Seq(BSR)to identify the key regulatory gene of fruit cracking in ***-Seq analysis illustrated that two regions associated with irregularly cracking were located on chromosomes 9 and 11,containing 127 candidate ***,through differentially expression analysis and qRT-PCR in cracking-susceptible and cracking-resistant genotypes,the candidate gene SlGH9-15(Solyc09g010210)with significantly differential expression levels was *** analysis of the GH9 gene family revealed that 20 SlGH9 genes were divided into three *** phylogenetic analysis showed that SlGH9-15 was closely related to cell wall construction-associated genes AtGH9B1,AtGH9B6,OsGH9B1,and *** cis-acting elements analysis revealed that Sl GH9-15 was activated by various hormones(ethylene and ABA)and abiotic *** expression pattern indicated that 13 SlGH9 genes,especially SlGH9-15,were highly expressed in the cracking-susceptible *** expression level gradually increased during fruit development and achieved maximum value at the red ripe ***,the cracking-susceptible tomato showed higher cellulase activity and lower cellulose content than the cracking-resistant tomato,particularly at the red ripe *** study identified SlGH9-15 as a key gene associated with fruit cracking in tomatoes for the first time and gives new insights for understanding the molecular mechanism and complex regulatory network of fruit cracking.

关键词： tomato irregular cracking BSR-Seq GH9-15

NRGA1, a Putative Mitochondrial Pyruvate Carrier, Mediates ABA Regulation of Guard cell Ion Channels and Drought Stress Responses in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2014年第10期7卷 1508-1521页

作者： Chun-Long Li Mei Wang Xiao-Yan Ma Wei Zhang key laboratory of plant cell engineering and germplasm innovation Ministry of Education School of Life Science Shandong UniversityJinan 250100 China

Abscisic acid （ABA） regulates ion channel activity and stomatal movements in response to drought and other stresses. Here, we show that the Arabidopsis thaliana gene NRGA1 is a putative mitochondrial pyruvate carrier which negatively regulates ABA-induced guard cell signaling. NRGA1 transcript was abundant in the A. thaliana leaf and par- ticularly in the guard cells, and its product was directed to the mitochondria. The heterologous co-expression of NRGA1 and AtMPC1 in yeast complemented a loss-of-function mitochondrial pyruvate carrier （MPC） mutant. The nrgal loss-of- function mutant was very sensitive to the presence of ABA in the context of stomatal movements, and exhibited a height- ened tolerance to drought stress. Disruption of NRGA1 gene resulted in increased ABA inhibition of inward K＋ currents and ABA activation of slow anion currents in guard cells. The nrgal/NRGA1 functional complementation lines restored the mutant＇s phenotypes. Furthermore, transgenic lines of constitutively overexpressing NRGA1 showed opposite stomatal responses, reduced drought tolerance, and ABA sensitivity of guard cell inward K＋ channel inhibition and anion channel activation. Our findings highlight a putative role for the mitochondrial pyruvate carrier in guard cell ABA signaling in response to drought.

关键词： NRGAI mitochondrial pyruvate carrier ABA guard cell stomata inward K+ channel slow anion channel drought stress.

Progress of chromosome engineering mediated by asymmetric somatic hybridization

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2009年第9期36卷 547-556页

作者： Guangmin Xia key laboratory of plant cell engineering and germplasm innovation of Education Ministry School of Life Sciences Shandong University Jinan 250100 China

plant somatic hybridization has progressed steadily over the past 35 years. Many hybrid plants have been generated from fusion combinations of different phylogenetic species, some of which have been utilized in crop breeding programs. Among them, asymmetric hybrid, which usually contains a fraction of alien genome, has received more attention because of its importance in crop improvement. However, few studies have dealt with the heredity of the genome of somatic hybrid for a long time, which has limited the progress of this approach. Over recent ten years, along with the development of an effective cytogenetical tool ＂in situ hybridization （ISH）＂, asymmetric fusion of common wheat （Triticum aestivum L.） with different grasses or cereals has been greatly developed. Genetics, genomes, functional genes and agricultt, ral traits of wheat asymmetric hybrids have been subject to systematic investigations using gene cloning, genomic in situ hybridization （GISH） and molecular makers. The future goal is to fully elucidate the functional relationships among improved agronomic traits, the genes and underlying molecular mechanisms, and the genome dynamics of somatic introgression lines. This will accelerate the development of elite germplasms via somatic hybridization and the application of these materials in the molecular improvement of crop plants.

关键词： asymmetric somatic hybrids GISH alien introgression wheat breading