检索结果-南通市图书馆

Chinese Journal of Structural Chemistry 2004年第11期23卷 1222-1225页

作者：朱松磊屠树江李团结邹翔章晓镜纪顺俊张勇 Department of Chemistry Xuzhou Normal UniversityKey Laboratory of Biotechnology on Medical Plant Xuzhou Jiangsu 221116 ChinaDepartment of Chemistry Xuzhou Medical College Xuzhou Jiangsu 221002 China Department of Chemistry Xuzhou Normal UniversityKey Laboratory of Biotechnology on Medical Plant Xuzhou Jiangsu 221116 China College of Chemistry and Chemical Engineering Key Lab. of Org. Synth of Jiangsu Province Suzhou University Suzhou Jiangsu 215006 China

The title compound (C20H15ClN4O, Mr = 362.81) was synthesized by the reaction of ethyl 2-cyano-3-(2-chlorophenyl)-1-acylate with 5-amino-3-methyl-1-phenylpyrazole under microwave irradiation and its structure was determined by single-crystal X-ray diffraction. The crystal is of monoclinic, space group P21/n with a = 8.8192(19), b = 15.976(3), c = 13.094(3) ? = 103.074(4), V = 1797.1(7) ?, Z = 4, Dc = 1.341 g/cm3, = 0.229 mm-1, F(000) = 752, R = 0.0632 and wR = 0.1278. X-ray analysis reveals that the pyridine ring adopts an envelope conformation. The dihedral angle between the pyrazole and pyridine planes is 2.19, and inter- molecular hydrogen bonds are also observed in the molecule.

关键词： aminopyrazole pyrazolopyridine crystal structure microwave irradiation

A virus-derived siRNA activates plant immunity by interfering with ROS scavenging

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2021年第7期14卷 1088-1103页

作者： Peng Liu Xiaoxiang Zhang Fan Zhang Miaoze Xu Zhuangxin Ye Ke Wang Shuang Liu Xiaolei Han Ye Cheng Kaili Zhong Tianye Zhang Linzhi Li Youzhi Ma Ming Chen Jianping Chen Jian Yang State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant VirologyNingbo UniversityNingbo 315211China lnstitute of Agricultural Sciences in Lixiahe District of Jiangsu province YangzhouJiangsu 225007China Yantai Academy of Agricultural Science Shandong ProvinceNo.26 Gangcheng West StreetFushan DistrictYantai CityShandong 265500P.R.China National key Facility for Crop Genetic Resources and Genetic Improvement Key Laboratory of Crop Genetics and BreedingMinistry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina

Virus-derived small interference RNAs(vsiRNAs)not only suppress virus infection in plants via induction of RNA silencing but also enhance virus infection by regulating host defensive gene ***,the underlying mechanisms that control vsiRNA-mediated host immunity or susceptibility remain largely *** this study,we generated several transgenic wheat lines using four artificial microRNA expression vectors carrying vsiRNAs from Wheat yellow mosaic virus(WYMV)*** and field tests showed that two transgenic wheat lines expressing amiRNAI were highly resistant to WYMV *** analyses showed that vsiRNAI could modulate the expression of a wheat thioredoxin-like gene(TaAAEDI),which encodes a negative regulator of reactive oxygen species(ROS)production in the *** function of TaAAEDI in ROS scavenging could be suppressed by vsiRNAI in a dose-dependent ***,transgenic expression of amiRNAI in wheat resulted in broad-spectrum disease resistance to Chinese wheat mosaic virus,Barley stripe mosaic virus,and Puccinia striiformis *** infection,suggesting that vsiRNAI is involved in wheat immunity via ROS ***,these findings reveal a previously unidentified mechanism underlying the arms race between viruses and plants.

关键词： vsiRNAI Wheat yellow mosaic virus TaAAEDI ROS host resistance

Identification of Polymorphic Markers by High-Resolution Melting(HRM)Assay for High-Throughput SNP Genotyping in Maize

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2021年第6期90卷 1711-1725页

作者： Zhigang Shang Yongzhe Zhu Xinmei Guo Meiai Zhao College of Agronomy Qingdao Agricultural UniversityQingdaoChina key laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application QingdaoChina College of Chemistry and Pharmacy Qingdao Agricultural UniversityQingdaoChina key laboratory of plant biotechnology in Universities of shandong province College of Life SciencesQingdao Agricultural UniversityQingdaoChina

The development of next generation sequencing(NGS)and high throughput genotyping are important techniques for the QTL mapping and genetic analysis of different ***-resolution melting(HRM)is an emerging technology used for detecting single-nucleotide polymorphisms(SNPs)in various ***,its use is still limited in *** HRM analysis was integrated with SNPs to identify three types of populations(NIL population,RIL population and natural population),and the useful tags were *** patterns of temperatureshifted melting curves were investigated from the HRM analysis,and compared these with the *** all 48 pairs of primers,10 pairs of them were selected:six pairs of primers for the NIL population,three pairs of primers for the RIL population,and one pair of primer for the natural *** marker for the natural population was developed with a matching rate of 80%for the plant height trait,based on the data of the phenotypic characteristics measured in the *** study provides an effective method for maize genotyping in the classification of maize germplasm resources,which can be applied to other plants for high-throughput SNP genotyping or further mapping.

关键词： Maize high-throughput genotyping HRM SNP

Increased salinity and groundwater levels lead to degradation of the Robinia pseudoacacia forest in the Yellow River Delta

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry Research 2022年第4期33卷 1233-1245页

作者： Chaoxia Lu Chen Zhao Jing Liu Kailun Li Baoshan Wang Min Chen shandong Provincial key laboratory of plant Stress Research College of Life ScienceShandong Normal UniversityJi’nan 250014People’s Republic of China shandong Provincial key laboratory of Crop Genetic Improvement Ecology and PhysiologyShandong Academy of Agricultural SciencesJi’nan 250100People’s Republic of China

Forest degradation is a worldwide problem,although its causes vary due to geographical and climatic differences and man-made *** recent years,the Robinia pseudoacacia forest in the Yellow River Delta has suffered severe *** causative mechanisms were investigated in the field over two years,and the results show that increased forest degradation was reflected by increased tree mortality,high leaf and soil sodium salt levels and groundwater *** tree diameters decreased,and leaf chlorophyll and soil microbial contents *** discriminate analysis(RDA)showed that degradation of the forest was correlated positively with soil salt content,but negatively with groundwater *** analysis showed that 0.79%–0.95%soil salt content and above 1.20 m groundwater depth caused the death of *** trees due to localized anthropogenic economic activities,such as rice farming,that disrupted the original water–salt *** are recommended to prevent further degradation and restore degraded forests.

关键词： Forest degradation Groundwater depth Robinia pseudoacacia forest Soil salt content The Yellow River Delta

Co-planted barnyardgrass reduces rice yield by inhibiting plant above-and belowground-growth during post-heading stages

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2021年第5期9卷 1198-1207页

作者： Zichang Zhang Jingjing Cao Tao Gu Xia Yang Qiong Peng Lianyang Bai Yongfeng Li Institute of plant Protection Jiangsu Academy of Agricultural SciencesKey Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding BaseNanjing 210014JiangsuChina biotechnology Research Center Hunan Academy of Agricultural SciencesChangsha 410125HunanChina

Barnyardgrass (Echinochloa spp.) is the most common noxious weed in rice paddies as it inhibits rice growth and reduces grain *** date,little information is available on above-and belowgroundgrowth changes in rice due to neighboring *** study aimed to investigate the changes in root traits and shoot growth of rice when it is grown with different kinds of *** rice plants (*** 9108) were co-cultured with two varieties of Echinochloa crusgalli (L.) Beauv.(EP,*** (pursh) Petern;EH,*** (H.B.K.) Hitchc),and *** (L.) Link (EL) in the field in 2017 and *** treatments included control (i.e.,weed free rice plants) and co-cultures with each of three barnyardgrasses (EP,EH,and EL).The results revealed that EP,EH,and EL treatments significantly reduced rice grain yields by 30.6%–36.2%,42.5%–46.5%,and 10.6%–14.3%,*** growth including shoot dry weight,leaf photosynthetic rate,zeatin (Z) and zeatin riboside (ZR) in grains,and activities of key enzymes involved in sucrose-to-starch conversion in grains and root traits,such as length density,root dry weight,total absorbing surface area,active absorption surface area,oxidation activity,and Z+ZR contents in roots were dramatically reduced during post-heading stages of rice when grown with the three kinds of ***,above-mentioned rice shoot growth indices were strongly and positively correlated with root *** results suggested the decrease in rice shoot growth and root traits during post-heading stages contributes to the reduction in the rice yield when it grows with barnyardgrass neighbors.

关键词： Rice Barnyardgrass Grain yield Root traits Shoot traits

Cloning and expression of the nuclear transcription factor SIβFTZ-F1 cDNA from Spodoptera fitura

在线全文

学校读者我要写书评

暂无评论

Insect Science 2011年第6期18卷 635-644页

作者： Lin Tang Chun Zhang Lin Liu Qili Feng Sichun Zheng Guangdong Provincial key laboratory of biotechnology for plant Development School of Life Sciences South China Normal University Guangzhou College of Life Sciences Hunan University of Arts and Sciences Changde Hunan Province China

Insect molting and metamorphosis are regulated by 20-hydroxyecdysone, which activates the ecdysone receptors, followed by expression of a series of transcription factors. Beta Fushi Tarazu-Factor 1 （βFTZ-F1） is one of these ecdysis-related earlyexpressed transcription factors. A βFTZ-F1 complementary DNA （cDNA） was cloned from Spodoptera litura. The full-length cDNA was 2 194 bp in length and the open reading frame ofS1βFTZ-F1 cDNA consisted of 1 659 bp and encoded a 552-amino acid protein with a molecular mass of 61.4 kDa. Homology analysis revealed that S1βFTZ-F 1 protein shared high identities to its homologues from other species. The A/B domains and the D domains were diverse among the insectβFTZ-F 1 proteins, but DNA binding domains （C domain） and the ligand binding domains （E domain） were highly conserved. Northern blot analysis detected a transcript of 2.2 kb in the epidermis of fifth to sixth molting larvae. Reverse transcription polymerase chain reaction revealed that SIβFTZ-F1 gene started to increasingly and periodically express before each molt, followed by a decrease during the intermolt stages.

关键词： ecdysis metamorphosis molt Spodoptera litura transcription factor

Spindle-E is essential for gametogenesis in the silkworm, Bombyx mori

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2023年第2期30卷 293-304页

作者： Kai Chen Xu Yang Dehong Yang Yongping Huang Jiangsu key laboratory of Sericultural Biology and biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina key laboratory of Insect Developmental and Evolutionary Biology Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina CAS Center for Excellence in Biotic Interactions University of the Chinese Academy of SciencesBejingChina

As a defense mechanism against transposable elements,the PIWI-interacting RNA(piRNA)pathway maintains genomic integrity and ensures proper gametogenesis in *** factors are orchestrated to ensure normal operation of the piRNA ***-E(Spn-E)gene was one of the first genes shown to participate in the piRNA *** this study,we performed functional analysis of Spn-E in the model lepidopteran insect,Bombyx *** the germline-specific expression pattern observed in Drosophila and mouse,BmSpn-E was ubiquitously expressed in all tissues tested,and it was highly expressed in *** staining showed that BmSpn-E was localized in both germ cells and somatic cells in ovary and was expressed in spermatocytes in *** used a binary transgenic CRISPR/Cas9 system to construct BmSpn-E *** of BmSpn-E expression caused derepression of transposons in *** also found that mutant gonads were much smaller than wild-type gonads and that the number of germ cells was considerably lower in mutant *** real-time PCR analysis and TUNEL staining revealed that apoptosis was greatly enhanced in mutant ***,we found that the BmSpn-E mutation impacted gonadal development and gametogenesis at the early larval *** summary,our data provided the first evidence that BmSpn-E plays vital roles in gonadal development and gametogenesis in ***.

关键词： Bombyx mori gametogenesis piRNA pathway Spindle-E

Virus-Encoded microRNAs: Future Therapeutic Targets?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2006年第6期3卷 411-419页

作者： Peng Qi Jinxiang Han Yanqin Lu Chuanxi Wang Fanfeng Bu shandong Medicinal biotechnology Center Shandong Academy of Medical Science Key laboratory of Ministry of Health for Biotech-Drug Jinan 250062 China School of Medicine Shandong University Jinan 250012 China

The discovery of microRNAs （miRNAs） is a remarkable breakthrough in the field of molecular genetics, as miRNAs are key actors which regulate gene expression in diverse cellular processes from unicellular yeast to human. The recent discovery of virus-encoded miRNAs indicates that viruses also use this fundamental mode of gene regulation. Research into viral miRNAs function demonstrates that some miRNAs play an important role in regulating both the viral life cycle and the interaction between viruses and their hosts. The first in vivo ＂antagomir＂ study provides an exciting first step towards miRNA therapy, and the potential for ultimately designing molecular medicines based on the modulation of miRNAs seems good.

关键词： microRNA gene expression anti-miRNA oligonucleotide therapeutic target antivirus

Synthesis and Crystal Structure of 4-(4-Chlorophenyl)-6-methyl-5-methoxy-caronyl-3,4-dihydropyrimidin-2(H)-one

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2004年第11期23卷 1295-1299页

作者：华国平屠树江房芳屠蔓苏史达清王香善纪顺俊张勇 Xuzhou Institute of Architectural Technology Xuzhou Jiangsu 221008 China Department of Chemistry Xuzhou Normal University Key Laboratory of Biotechnology on Medical Plant of Jiangsu Province Xuzhou Jiangsu 221009 China College of Chemistry and Chemical Engineering Key Lab of Organic Synthesis of Jiangsu Province Suzhou University Jiangsu 215006 China

The title compound 4-(4-chlorophenyl)-6-methyl-5-methoxy-caronyl-3,4-dihydro- pyrimidin-2(H)-one has been synthesized by the reaction of 4-chlorobenzaldehyde, methyl acetoa- cetate and urea using H3BO3 as a catalyst under microwave irradiation. Its structure was charac- terized by X-ray single-crystal diffraction techniques with the following data: triclinic, space group P1, a = 5.4513(4), b = 7.619(1), c = 16.118(4) ?, α = 80.42(1), β = 83.43(1), γ = 79.29(1)°, V = 646.19(19) ?3, Mr = 280.70, μ(MoKα) = 0.301 mm-1, Z = 2 and Dc = 1.443 g/cm3. In the molecule the pyrimidine ring is of boat conformation.

关键词： microwave irradiation 3,4-dihydropyrimidinone H3BO3 crystal structure

Quantitative Trait Locus Mapping of High Photosynthetic Efficiency and Biomass in Oryza longistaminata

在线全文

学校读者我要写书评

暂无评论

Rice science 2022年第6期29卷 569-576,I0034,I0035页

作者： SI Fengfeng FAN Fengfeng WEI Xiao HE Shihao LI Xianlong PENG Xiaojue LI Shaoqing State key laboratory of Hybrid Rice/Hongshan laboratory of Hubei province/key laboratory for Research and Utilization of Heterosis in indica Rice of Ministry of Agriculture and Rural Affairs/Engineering Research Center for plant biotechnology and Germplasm Utilization of Ministry of Education/College of Life Science Wuhan UniversityWuhan 430072China key laboratory of Molecular Biology and Gene Engineering of Jiangxi province/College of Life Science Nanchang UniversityNanchang 330031China

Photosynthetic efficiency,a key trait that determines yield potential in rice,is quantitatively regulated by multiple *** of valuable genetic resources hidden in wild rice is an effective way to improve rice photosynthesis and yield *** this study,152 backcross inbred lines derived from wild rice Oryza longistaminata were explored for QTL mapping of photosynthetic rate(Pn)and biomass(BM)in natural *** novel QTLs for Pn and seven QTLs for BM or daily biomass(DBM)derived from *** were *** of these QTLs,qPn8.1,could significantly improve Pn and was located in a 68-kb region containing only 11 candidate ***,qBM1.1 and qDBM1.1 for BM and DBM on chromosome 1 were overlapped with qPn1.1 for Pn from 9311,and could affect both Pn and BM in natural *** QTLs identified in *** may provide a novel alternative to explore new genes and resources for yield potentiality,highlighting the important role of wild rice in rice breeding programs.

关键词： QTL Oryza longistaminata photosynthetic rate biomass wild rice