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The miR3367–lncRNA67–GhCYP724B module regulates male sterility by modulating brassinosteroid biosynthesis and interacting with Aorf27 in Gossypium hirsutum

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Journal of Integrative plant Biology 2025年第1期67卷 169-190页

作者： Anhui Guo Hushuai Nie Huijing Li Bin Li Cheng Cheng Kaiyun Jiang Shengwei Zhu Nan Zhao Jinping Hua laboratory of Cotton Genetics Genomics and Breeding/Joint Laboratory for International Cooperation in Crop Molecular BreedingMinistry of Education/Beijing Key Laboratory of Crop Genetic ImprovementCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China key laboratory of plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China

Cytoplasmic male sterile(CMS)lines play a crucial role in utilization of heterosis in crop ***,the mechanism underlying the manipulation of male sterility in cotton by long non-coding RNA(lncRNA)and brassinosteroids(BRs)remains ***,using an integrative approach combining lncRNA transcriptomic profiles with virus-induced gene silencing experiments,we identify a flower budspecific lncRNA in the maintainer line 2074B,lncRNA67,negatively modulating with male sterility in upland cotton(Gossypium hirsutum).lncRNA67positively regulates cytochrome P274B(GhCYP724B),which acted as an eTM(endogenous target mimic)for *** suppression of GhCYP724B induced symptoms of BR deficiency and male semisterility in upland cotton as well as in tobacco,which resulted from a reduction in the endogenous BR ***724B regulates BRs synthesis by interacting with GhDIM and GhCYP90B,two BRs biosynthesis ***,GhCYP724B suppressed a unique chimeric open reading frame(Aorf27)in 2074A mitochondrial *** expression of Aorf27 in yeast inhibited cellular growth,and over expression of Aorf27 in tobacco showed male ***,the results proved that the miR3367–lncRNA67–GhCYP724B module positively regulates male sterility by modulating BRs *** findings uncovered the function of lncRNA67–GhCYP724B in male sterility,providing a new mechanism for understanding male sterility in upland cotton.

关键词： brassinosteroids GhCYP724B lncRNA67 male sterility mitochondrial Aorf27 upland cotton(G.hirsutum)

Efficient gene disruption in polyploid genome by Cas9–Trex2 fusion protein

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Journal of Integrative plant Biology 2025年第1期67卷 7-10页

作者： Wenbo Pan Chunlei Gao De Niu Jinghua Cheng Jiao Zhang Xiying Yan Qiang Long Yao Yao Zhu Wenjing Sun Qi Xie Yuehui He Xing Wang Deng Huawei Zhang Jian Li State key laboratory of Wheat Improvement Shandong Laboratory of Advanced Agricultural Sciences in WeifangPeking University Institute of Advanced Agricultural SciencesWeifang 261325China Guangdong Provincial key laboratory of biotechnology for plant Development Ministry of Education&Guangdong Provincial Key Laboratory of Laser Life ScienceSchool of Life SciencesSouth China Normal UniversityGuangzhou 510631China Shenzhen Tidy field System biotechnology Co. LtdShenzhen 518107China State key laboratory of plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China

Despite significant advancements in the efficiency of the CRISPR/Cas9 genome editing system over the past decade,performing gene disruption in polyploid crops such as common wheat(Triticum aestivum L.)remains challeng... 详细信息

关键词： CRISPR/Cas9 crops trait

Intestinal Flatulence in Pampus argenteus:Changes in Gut Microbiota

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Journal of Ocean University of China 2025年第1期24卷 182-194页

作者： NIE Wenhao ZHANG Man ZHANG Youyi LI Yuanbo XU Shanliang HU Jiabao WANG Yajun YAN Xiaojun key laboratory of Applied Marine biotechnology Ministry of EducationNingbo UniversityNingbo 315832China key laboratory of Marine biotechnology of Zhejiang Province Ningbo UniversityNingbo 315832China College of Marine Sciences Ningbo UniversityNingbo 315832China

Pampus argenteus is a commercially valuable species in many *** artificial cultivation has been impacted by intestinal flatulence,leading to increased ***,the role of gut microbiota in this process remains ***,this study utilized high-throughput sequencing of 16S rRNA genes to investigate the gut microbiota in both flatulent and healthy ***.A total of 227571 high-quality and classifiable reads,representing 312 Amplicon Sequence Variants(ASVs),were *** emerged as the predominant phylum in all samples,with the flatulent group displaying the highest relative abundance(85.3%).Microbial diversity was significantly greater in samples from water than those from *** analysis using Bray-Curtis distance via CPCoA revealed distinct gut microbiota compositions between the samples with flatulence and the healthy ***-occurrence network analysis demonstrated that the bacterial community in the flatulent group exhibited disorder due to the high abundance of *** predictive analysis(PICRUSt2)indicated that flatulence in *** was associated with impaired methane and lipid metabolism,potentially resulting in excessive methane synthesis utilizing hydrogen and acetic acid as substrates produced by Clostridium sensu stricto 1,Cetobacterium,and *** study holds significant implications for early diagnosis and prevention of intestinal flatulence through the regulation of gut microbiota.

关键词： Pampus argenteus gut microbiota 16S rRNA intestinal flatulence PICRUSt2

A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer,Ostrinia furnacalis

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Insect Science 2025年第1期32卷 172-192页

作者： Er-Tao Li Jia-Yue Ji Wei-Jie Kong Dong-Xu Shen Cai Li Chun-Ju An Deparment of Entomology College of Plant ProtectionChina Agricultural UniversityBejingChina Pomology Institute Shanxi Agricultural UniversityJinzhongShanxi ProvinceChina Jiangsu key laboratory of Sericultural Biology and biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina

C-type lectins(CTLs)act as pattern recognition receptors(PRRs)to initiate the innate immune response in insects.A CTL with dual carbohydrate recognition domains(CRDs)(named immulectin-4[IML-4])was selected from the Ostrinia furnacalis transcriptome dataset for functional *** cloned the full-length complementary DNA of *** IML-4(OfIML-4).It encodes a 328-residue protein with a Glu-Pro-Asn(EPN)and Gln-Pro-Asp(QPD)motifs in 2 CRDs,***-4 messenger RNA levels increased significantly upon the bacterial and fungal *** OfIML-4(rIML-4)and its individual CRDs(rCRD1 and rCRD2)exhibited the binding ability to various microorganisms including Escherichia coli,Micrococcus luteus,Pichia pastoris,and Beauveria bassiana,and the cell wall components including lipopolysaccharide from ***,peptidoglycan from *** or Bacillus subtilis,and curdlan from Alcaligenes *** binding further induced the agglutination of ***,***,and *** in the presence of calcium,the phagocytosis of Staphylococcus aureus by the hemocytes,in vitro encapsulation and melanization of nickel-nitrilotriacetic acid beads,and a significant increase in phenoloxidase activity of *** addition,rIML-4 significantly enhanced the phagocytosis,nodulation,and resistance of *** to *** together,our results suggest that OfIML-4 potentially works as a PRR to recognize the invading microorganisms,and functions in the innate immune response in ***.

关键词： cellular immunity C-type lectin humoral immunity immulectin Ostrinia furnacalis pattern recognition receptor(PRR)

Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2025年第1期68卷 308-316页

作者： Sili Wang Jiancheng Huang Yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong Yuanyuan Shi Bin Hong Xiaoying Bian Wen Liu State key laboratory of Chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200032China State key laboratory of Microbial Technology Shandong UniversityQingdao 266237China National Health Commission(NHC)key laboratory of biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science&Peking Union Medical CollegeBeijing 100050China

Cobalamin(Cbl)-dependent radical S-adenosyl-L-methionine(SAM)proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual *** of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding *** the biosynthetic pathway of chuangxinmycin(CXM),a unique indole alkaloid antibiotic with potent anti-infective activity,functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process,which,however,remained to be reconstituted *** here report the dissection of this enzymatic process,which requires the incorporation of Cxm8,a Cbl-dependent radical SAM protein,with Cxm9,a DUF5825family protein that shares no homology to any proteins of known ***8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner,achieving CXM and a recently identified,C3-dimethylated *** biochemical characterization,isotope labeling,structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase *** is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

关键词： cbl-dependent radical SAM protein biosynthesis chuangxinmycin stereoselective methylation

Recognition of a salivary effector by the TNL protein RCSP promotes effector-triggered immunity and systemic resistance in Nicotiana benthamiana

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Journal of Integrative plant Biology 2025年第1期67卷 150-168页

作者： Weiwei Rao Tingting Ma Jiayuan Cao Yajun Zhang Sisi Chen Shu Lin Xiaoxiao Liu Guangcun He Li Wan key laboratory of plant Design CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China National key laboratory of Hybrid Rice College of Life SciencesWuhan UniversityWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China

Insects secret chemosensory proteins(CSPs)into plant cells as potential effector proteins during *** molecular mechanisms underlying how CSPs activate plant immunity remain largely *** show that CSPs from six distinct insect orders induce dwarfism when overexpressed in Nicotiana ***-mediated transient expression of Nilaparvata lugens CSP11(NlCSP11)triggered cell death and plant dwarfism,both of which were dependent on ENHANCED DISEASE SUSCEPTIBILITY 1(EDS1),N requirement gene1(NRG1)and SENESCENCE-ASSOCIATED GENE 101(SAG101),indicating the activation of effector-triggered immunity(ETI)in *** of NlCSP11 led to stronger systemic resistance against Pseudomonas syringae DC3000 lacking effector HopQ1-1 and tobacco mosaic virus,and induced higher accumulation of salicylic acid(SA)in uninfiltrated leaves compared to another effector XopQ that is recognized by a Tollinterleukin-1 receptor(TIR)domain nucleotidebinding leucine-rich repeat receptor(TNL)called ROQ1 in ***,NlCSP11-induced dwarfism and systemic resistance,but not cell death,were abolished in *** transgenic line expressing the SA-degrading enzyme Nah *** large-scale virus-induced gene silencing screening,we identified a TNL protein that mediates the recognition of CSPs(RCSP),including aphid effector MP10 that triggers resistance against aphids in ***,bimolecular fluorescence complementation and Alpha Fold2 prediction unveiled an interaction between NlCSP11 and ***,RCSP does not contain the conserved catalytic glutamic acid in the TIR domain,which is required for TNL *** findings point to enhanced ETI and systemic resistance by a TNL protein via hyperactivation of the SA ***,RCSP is the first TNL identified to recognize an insect effector.

关键词： chemosensory protein effector effector-triggered immunity salicylic acid TIR-NBS-LRR

Phosphorylation modification reverses the transcriptional inhibitory activity of CsMYB4a in tea plants(Camellia sinensis)

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Horticultural plant Journal 2025年第1期11卷 406-416页

作者： Guoliang Ma Yunyun Han Tongtong Li Tao Xia Liping Gao School of Life Science Anhui Agricultural UniversityHefeiAnhui 230036China State key laboratory of Tea plant Biology and Utilization Key Laboratory of Tea Biology and Tea Processing of Ministry of AgricultureAnhui Provincial Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefeiAnhui 230036China

The members of the fourth subgroup of R2R3-MYB(Sg4 members)are well-known inhibitors of phenylpropanoid and lignin synthesis *** C2 domain is closely related to the transcriptional inhibitory activity of Sg4 *** modification enhances the transcriptional inhibitory activity of Sg4 ***,we identified a phosphorylation site on the C2 domain of Cs MYB4a from tea plants(Camellia sinensis).A mitogen-activated protein kinase(MAPK),named Cs MPK3-2,phosphorylated this site on the C2 domain of Cs *** experiments revealed that phosphorylation of Cs MYB4a weakened its ability to inhibit the gene expression of PAL,C4H,and 4CL in the phenylpropanoid pathway and activated the expression of transcription factor YABBY5,maintaining the adaxial-abaxial polarity of the *** out Nt YAB5 in Cs MYB4a transgenic tobacco partially repaired the leaf wrinkling phenotype caused by Cs *** C1 domain exhibited an activation function when the C2 domain of Cs MYB4a was phosphorylated by Cs MPK3-2,causing this reversal *** results enrich our understanding of the regulatory diversity of Sg4 members.

关键词： Camellia sinensis Cs MYB4a Phenylpropanoid pathway MAPK YABBY Leaf development

The phytohormone jasmonic acid is involved in rice resistance to Southern rice black-streaked dwarf virus

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The Crop Journal 2025年第1期13卷 23-30页

作者： Chaorui Huang Qing Liu Qingling Qi Chenfei Gao Lulu Li Yanjun Li Jianping Chen Zongtao Sun Jianlong Xu Hehong Zhang State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingbo 315211ZhejiangChina Institute of Crop Sciences National Key Facility for Crop Gene Resources and Genetic lmprovementChinese Academy of Agricultural SciencesBeijing 100081China

Viruses are significant pathogens causing severe plant infections and crop losses *** resistance mechanisms of rice to viral diseases,particularly Southern rice black-streaked dwarf virus(SRBSDV),remain poorly *** this study,we assessed SRBSDV susceptibility in 20 Xian/indica(XI)and 20 Geng/japonica(GJ)rice ***-1B accessions in the Xian subgroup displayed higher resistance than GJ *** transcriptome analysis revealed changes in processes like oxidoreductase activity,jasmonic acid(JA)metabolism,and stress *** sensitivity assays further linked antiviral defense to the JA *** findings highlight a JA-mediated resistance mechanism in rice and offer insights for breeding SRBSDV-resistant varieties.

关键词： Oryza sativa(rice) Jasmonic acid Southern rice black-streaked dwarf virus(SRBSDV) Variety resistance Defense response

Eukaryotic translation initiation factor OseIF5A4 negatively regulates rice immunity against blast and bacterial blight diseases

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The Crop Journal 2025年第1期13卷 79-91页

作者： Hui Wang Yuqing Yan Yan Bi Dayong Li Fengming Song National key laboratory for Rice Biology and Breeding Institute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina Ministry of Agriculture key laboratory of Molecular Biology of Crop Pathogens and Insect Pests Institute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina key laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province Institute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina

The RING-type E3 ligase OsBBI1 regulates rice resistance against Magnaporthe oryzae through modifying cell wall *** this study,we report the function of an OsBBI1 substrate,eukaryotic translation initiation factor OseIF5A4,in rice ***1 interacts with OseIF5A4 and other four members of the OseIF5A *** RING domain in OsBBI1 and the eIF-5a domain in OseIF5A4 are critical for the OsBBI1-OseIF5A4 ***1 ubiquitinates OseIF5A4 and mediates its degradation in vitro and in ***,the expression of OseIF5A4 was upregulated during early stage of compatible interaction but downregulated in incompatible interaction between rice and *** of OseIF5A4 enhances rice immunity against *** and Xanthomonas oryzae ***,boosts pattern-triggered immune responses,and strengthens pathogen-induced defense responses(e.g.,expression of defense genes,accumulation of reactive oxygen species and reinforcement of cell wall).However,overexpression of OseIF5A4 attenuates rice immunity and immune *** results demonstrate that OseIF5A4,a substrate of the immunity-associated E3 ligase OsBBI1,negatively regulates rice immunity against *** and *** *** through modulating pathogen-induced defense responses,highlighting the importance of the protein translational machinery in rice immunity.

关键词： Magnaporthe oryzae OsBBI1 OseIF5A Rice immunity Xanthomonas oryzae pv.oryzae