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Dynamic intrauterine crosstalk promotes porcine embryo implantation during early pregnancy

Science China(Life Sciences) 2024年第8期67卷 1676-1696页

作者： Xupeng Zang Shengchen Gu Wenjing Wang Junsong Shi Jianyu Gan Qun Hu Chen Zhou Yue Ding Yanjuan He Lei Jiang Ting Gu Zheng Xu Sixiu Huang Huaqiang Yang Fanming Meng Zicong Li Gengyuan Cai Linjun Hong Zhenfang Wu State key laboratory of Swine and Poultry breeding Industry National Engineering Research Center for Breeding Swine IndustryCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China Guangdong Provincial key laboratory of Agro-Animal Genomics and Molecular breeding South China Agricultural UniversityGuangzhou 510642China Yunfu Subcenter of Guangdong laboratory for Lingnan Modern Agriculture Yunfu 527300China Guangdong key laboratory of Animal breeding and Nutrition Institute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640China key laboratory of South China Modern Biological Seed Industry Ministry of Agriculture and Rural AffairsGuangzhou 510520China

Dynamic crosstalk between the embryo and mother is crucial during ***,we comprehensively profile the single-cell transcriptome of pig peri-implantation embryos and corresponding maternal endometrium,identifying 4 different lineages in embryos and 13 cell types in the ***-specific gene expression characterizes 4 distinct trophectoderm subpopulations,showing development from undifferentiated trophectoderm to polar and mural *** expression of genes in different types of endometrial cells illustrates their molecular response to embryos during ***,we developed a novel tool,ExtraCellTalk,generating an overall dynamic map of maternal-foetal crosstalk using uterine luminal proteins as *** cross-species comparisons,we identified a conserved RBP4/STRA6 pathway in which embryonic-derived RBP4 could target the STRA6 receptor on stromal cells to regulate the interaction with other endometrial *** results provide insight into the maternal-foetal crosstalk during embryo implantation and represent a valuable resource for further studies to improve embryo implantation.

关键词： endometrial remodeling ExtraCellTalk implantation maternal-foetal crosstalk trophoblast

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Genome-wide association study identifies 12 new genetic loci associated with growth traits in pigs

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Journal of Integrative Agriculture 2024年第1期23卷 217-227页

作者： Mu Zeng Binhu Wang Lei Liu Yalan Yang Zhonglin Tang Guangdong Provincial key laboratory of Animal Molecular Design and Precise breeding/key laboratory of Animal Molecular Design and Precise breeding of Guangdong Higher Education Institutes School of Life Science and EngineeringFoshan UniversityFoshan 528231China Kunpeng Institute of Modern Agriculture at Foshan Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesFoshan 528200China Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Livestock and Poultry Multi-omics of Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124China

Growth traits are among the most important economic traits in pigs and are regulated by polygenes with complex regulatory *** the major indicators of growth performance,the backfat thickness(BFT),loin eye area(LEA),and days to 100 kg(D100)traits are commonly used to the genetics improvement in ***,the available genetic markers for these traits are *** uncover novel loci and candidate genes associated with growth performance,we collected the phenotypic information of BFT,LEA,and D100 in 1,186 pigs and genotyped all these individuals using the Neogen GGP porcine 80K *** performed a genome-wide association study(GWAS)using 4 statistical models,including mixed linear models(MLM),fixed and random model circulating probability unification(FarmCPU),settlement of MLM under progressively exclusive relationships(SUPER),Bayesian-information and linkage-disequilibrium Iteratively nested keyway(Blink),and identified 5,3,and 6 high-confidence single nucleotide polymorphisms(SNPs)associated with BFT,LEA,and D100,*** annotation and quantitative trait locus(QTL)mapping analysis suggested that6 genes(SKAP2,SATB1,PDE7B,PPP1R16B,WNT3,and WNT9B)were potentially associated with growth performance in *** analysis suggested that the expression of Src Kinase Associated Phosphoprotein 2(SKAP2)was higher in prenatal muscles than in postnatal muscles,and the expression of Phosphodiesterase 7B(PDE7B)continuously increased during the prenatal stages and gradually decreased after birth,implying their potential roles in prenatal skeletal muscle ***,this study provides new candidate loci and genes for the genetic improvement of pigs.

关键词： GWAS pig growth trait QTL mapping skeletal muscle

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Versatile and efficient mammalian genome editing with Type I-C CRISPR System of Desulfovibrio vulgaris

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Science China Life Sciences 2024年

作者： Pan Li Dingcai Dong Fei Gao Yuyang Xie Honglin Huang Siwei Sun Zhao Ma Cheng He Jinsheng Lai Xuguang Du Sen Wu State key laboratory of Animal Biotech breeding College of Biological SciencesNational Engineering Laboratory for Animal BreedingFrontiers Science Center for Molecular Design BreedingChina Agricultural University College of Veterinary Medicine China Agricultural University Sanya Institute of China Agricultural University State key laboratory of Maize Bio-breeding National Maize Improvement CenterDepartment of Plant Genetics and BreedingChina Agricultural University Frontiers Science Center for Molecular Design breeding China Agricultural University Center for Crop Functional Genomics and Molecular breeding China Agricultural University

CRISPR-Cas tools for mammalian genome editing typically rely on single Cas9 or Cas12a *** type I CRISPR systems in Class I may offer greater specificity and versatility,they are not well-developed for genome ***,we present an alternative type I-C CRISPR system from Desulfovibrio vulgaris (Dvu) for efficient and precise genome editing in mammalian cells and *** optimized the Dvu type I-C editing complex to generate precise deletions at multiple loci in various cell lines and pig primary fibroblast cells using a paired PAM-in crRNA *** edited pig cells can serve as donors for generating transgenic cloned *** Dvu type I-C editor also enabled precise large fragment replacements with homology-directed ***,we adapted the Dvu-Cascade effector for cytosine and adenine base editing,developing Dvu-CBE and Dvu-ABE *** systems efficiently induced C-to-T and A-to-G substitutions in human genes without double-strand ***-target analysis confirmed the high specificity of the Dvu type I-C *** findings demonstrate the Dvu type I-C editor′s potential for diverse mammalian genome editing applications,including deletions,fragment replacement,and base editing,with high efficiency and specificity for biomedicine and agriculture.

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Effect of vacuum negative pressure aerobic hydrolysis pretreatment on corn stover anaerobic fermentation

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International Journal of Agricultural and Biological engineering 2023年第2期16卷 241-248页

作者： Yonghua Xu Yunong Song Hao Jiang Hongqiong Zhang Yong Sun Northeast Agriculture University Harbin 150030China key laboratory of pig-breeding facilities engineering Ministry of AgricultureHarbin 150030China

Lignin degradation restricts corn stover anaerobic fermentation *** vacuum negative pressure aerobic hydrolysis pretreatment of corn stover was tested,and the optimal combined pretreatment conditions were presented in this *** of the physical characteristics of light weight and large specific porosity of stover,it led to the formation of a scum layer during the fermentation process and thus reduced the gas production *** the pretreatment design,the vacuum conditions(0.02-0.08 MPa)and dwell time(5-20 min)were selected to see the changes of volumetric weight,swelling and specific porosity of corn stover,resulting in an increase of the volumetric weight by 7.18%-28.72%,an increase of the swelling by 3.18%-58.59%,and a decrease of the specific porosity by 9.34%-38.59%,as compared with the CK *** vacuum negative pressure treatment could discharge the air inside the stover destroy the microstructure,and cause the stover to settle more easily during the aerobic hydrolysis *** optimal aerobic hydrolysis temperature and time were determined to be 39°C and 12.65 h,*** the optimal pretreatment,the corn stover anaerobic fermentation test realized a cumulative methane yield of 260.44 mL/g VS,22.71%higher than CK group;meanwhile,the hydraulic retention time was shortened by 32.39%.

关键词： corn stover vacuum negative pressure pretreatment aerobic hydrolysis anaerobic fermentation

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Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第8期24卷 734-748页

作者： Jun HU Qiliang HOU Wenyong ZHENG Tao YANG Xianghua YAN National key laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070China The Cooperative Innovation Center for Sustainable pig Production Wuhan 430070China Hubei Provincial engineering laboratory for pig Precision Feeding and Feed Safety Technology Wuhan 430070Chin

A growing body of evidence has linked the gut microbiota to liver *** manipulation of intestinal microflora has been considered as a promising avenue to promote liver ***,the effects of Lactobacillus gasseri LA39,a potential probiotic,on liver metabolism remain *** studies have investigated the proteomic profile for mining the host biological events affected by microbes,and used the germ-free(GF)mouse model to evaluate host-microbe ***,we explored the effects of *** LA39 gavage on the protein expression profiles of the liver of GF *** results showed that a total of 128 proteins were upregulated,whereas a total of 123 proteins were downregulated by treatment with *** *** bioinformatics analyses suggested that the primary bile acid(BA)biosynthesis pathway in the liver was activated by *** *** differentially expressed proteins(cytochrome P450 family 27 subfamily A member 1(CYP27A1),cytochrome P450 family 7 subfamily B member 1(CYP7B1),and cytochrome P450 family 8 subfamily B member 1(CYP8B1))involved in the primary BA biosynthesis pathway were further validated by western blot *** addition,targeted metabolomic analyses demonstrated that serum and fecalβ-muricholic acid(a primary BA),dehydrolithocholic acid(a secondary BA),and glycolithocholic acid-3-sulfate(a secondary BA)were significantly increased by *** ***,our data revealed that *** LA39 activates the hepatic primary BA biosynthesis and promotes the intestinal secondary BA *** on these findings,we suggest that *** LA39 confers an important function in the gut‒liver axis through regulating BA metabolism.

关键词： Lactobacillus gasseri LA39 Liver Isobaric tags for relative and absolute quantitation(iTRAQ) Bile acid Germ-free mice

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Multi‑omics analysis reveals gut microbiota‑ovary axis contributed to the follicular development difference between Meishan and Landrace×Yorkshire sows

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畜牧与生物技术杂志:英文版 2023年第5期14卷 1954-1968页

作者： Baoyang Xu Wenxia Qin Yuwen Chen Yimei Tang Shuyi Zhou Juncheng Huang Libao Ma Xianghua Yan State key laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina The Cooperative Innovation Center for Sustainable pig Production Wuhan 430070HubeiChina Hubei Provincial engineering laboratory for pig Precision Feeding and Feed Safety Wuhan 430070HubeiChina

Background The mechanism by which Meishan(MS)sows are superior to white crossbred sows in ovarian follicle development remains *** gut microbiota could regulate female ovarian function and reproductive capacity,this study aimed to determine the role of gut microbiota-ovary axis on follicular development in *** We compared the ovarian follicular development,gut microbiota,plasma metabolome,and follicular fluid metabolome between MS and Landrace×Yorkshire(L×Y)sows.A H_(2)O_(2)-induced cell apoptosis model was used to evaluate the effects of multi-omics identified metabolites on the apoptosis of porcine ovarian granulosa cells in *** Compared with L×Y sows,MS sows have greater ovary weight and improved follicular development,including the greater counts of large follicles of diameter≥5 mm,secondary follicles,and antral follicles,but lesser atretic *** ovarian granulosa cells in MS sows had alleviated apoptosis,which was indicated by the increased BCL-2,decreased caspases-3,and decreased cleaved caspases-3 than in L×Y *** ovarian follicular fluid of MS sows had higher concentrations of estradiol,progesterone,follicle-stimulating hormone,luteinizing hormone,and insulin like growth factor 1 than L×Y *** microbiota of MS sows formed a distinct cluster and had improved alpha diversity,including increased Shannon and decreased Simpson than those of L×Y *** to the enhanced function of carbohydrate metabolism and elevated short-chain fatty acids(SCFAs)in feces,the differential metabolites in plasma between MS and L×Y sows are also mainly enriched in pathways of fatty acid *** were significant correlations among SCFAs with follicular development,ovarian granulosa cells apoptosis,and follicular fluid hormones,***,compared with L×Y sows,MS sows had higher follicular fluid SCFAs concentrations which could ameliorate H_(2)O_(2)-induced porcine granulosa cells apoptosis in ***

关键词： Follicular development Granulosa cells apoptosis Gut microbiota Short-chain fatty acids Sows

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Integrated analysis of multi-tissues lipidome and gut microbiome reveals microbiota-induced shifts on lipid metabolism in pigs

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Animal Nutrition 2022年第3期10卷 280-293页

作者： Chunlin Xie Xiaoyan Zhu Baoyang Xu Yaorong Niu Xuelei Zhang Libao Ma Xianghua Yan State key laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhanHubei 430070China The Cooperative Innovation Center of Sustainable pig Production WuhanHubei 430070China Hubei Provincial engineering laboratory for pig Precision Feeding and Feed Safety Technology WuhanHubei 430070China

Lipid metabolism is very important for meat quality in *** evidence shows that gut microbiota can contribute to this physiological ***,the gut microbiota that function in lipid metabolism and adipogenesis remains ***,we compared the characteristics of fat deposition and gut microbial community between Laiwu pigs and Duroc(LandraceYorkshire)(DLY)*** microbiota transplantation(FMT)was performed to determine the possible impact of gut microbiota on lipid metabolism in *** integrated analysis of the gut microbiome and lipidome of the small intestine,plasma,and liver was conducted to investigate the effects of FMT on host lipid *** comparative analysis of the gut microbiome showed higher abundance of Bacteroidetes(P=0.0018)while lower abundance of Firmicutes(P=0.012)in Laiwu pigs,and the microbial composition can be transferred from Laiwu pigs into DLY *** electron microscope and Oil red-O staining were performed to analyze the effects of FMT on lipid deposition in liver,the main target organ for lipid *** results showed that FMT significantly increased the number of lipid droplets(P=0.0035)and lipid accumulation(P=0.0026)in ***,integrated multi-tissues lipidome analysis demonstrated that the fatty acyls and glycerophospholipids were significantly increased(Ppigs.

关键词： pig Gut microbiota Lipidome Fecal microbiota transplantation Lipid metabolism

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Identification of gut microbes associated with feed efficiency by daily-phase feeding strategy in growing-finishing pigs

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Animal Nutrition 2023年第1期12卷 42-53页

作者： Qin Jiang Chunlin Xie Lingli Chen Hongli Xiao Zhilian Xie Xiaoyan Zhu Libao Ma Xianghua Yan State key laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhanHubei 430070China The Cooperative Innovation Center of Sustainable pig Production WuhanHubei 430070China Hubei Provincial engineering laboratory for pig Precision Feeding and Feed Safety Technology WuhanHubei 430070China

Feed efficiency is one of the most important issues for sustainable pig ***-phase feeding(DPF)is a form of precision feeding that could improve feed efficiency in *** microbiota can regulate host nutrient digestion,absorption,and ***,which key microbes may play a vital role in improving the feed efficiency during DPF remains *** the present study,we used a DPF program compared to a three-phase feeding(TPF)program in growing-finishing pigs to investigate the effects of gut microbiota on feed efficiency.A total of 204 Landrace×Yorkshire pigs(75 d)were randomly assigned into 2 *** treatment was replicated 8 times with 13 to 15 pigs per replicate *** in the TPF group were fed with a commercial feeding program that supplied fixed feed for phasesⅠ,Ⅱ,andⅢ,starting at 81,101,and 132 d of age,respectively,and pigs in the DPF group were fed a blend of adjacent phase feed from 81 to 155 d at a gradual daily ratio and phaseⅢfeed from 155 to 180 d of *** feed intake and body weight were recorded by a computerized device in the *** and blood samples were collected from 1 pig per replicate at 155 and 180 d of *** results showed that the DPF program remarkably improved the feed efficiency at 155 d(Pkey microbes may contribute to feed efficiency during daily-phase feeding strategies in pigs.

关键词： Daily-phase feeding Feed efficiency Gut microbiota Nutrient metabolism pig

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Rice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第5期24卷 430-441页

作者： Juncheng HUANG Wenxia QIN Baoyang XU Haihui SUN Fanghua JING Yunzheng XU Jianan ZHAO Yuwen CHEN Libao MA Xianghua YAN State key laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070China The Cooperative Innovation Center for Sustainable pig Production Wuhan 430070China Hubei Provincial engineering laboratory for pig Precision Feeding and Feed Safety Wuhan 430070China Yichun Dahaigui Life Science Co. Ltd.Yichun 336000China

Early weaned piglets suffer from oxidative stress and enteral infection,which usually results in gut microbial dysbiosis,serve diarrhea,and even *** bran oil(RBO),a polyphenol-enriched by-product of rice processing,has been shown to have antioxidant and anti-inflammatory properties both in vivo and in ***,we ascertained the proper RBO supplementation level,and subsequently determined its effects on lipopolysaccharide(LPS)-induced intestinal dysfunction in weaned piglets.A total of 168 piglets were randomly allocated into four groups of seven replicates(42 piglets each group,(21±1)d of age,body weight(7.60±0.04)kg,and half males and half females)and were given basal diet(Ctrl)or basal diet supplemented with 0.01%(mass fraction)RBO(RBO1),0.02%RBO(RBO2),or 0.03%RBO(RBO3)for 21 ***,seven piglets from the Ctrl and the RBO were treated with LPS(100μg/kg body weight(BW))as LPS group and RBO+LPS group,***,seven piglets from the Ctrl were treated with the saline vehicle(Ctrl group).Four hours later,all treated piglets were sacrificed for taking samples of plasma,jejunum tissues,and *** results showed that 0.02%was the optimal dose of dietary RBO supplementation based on diarrhea,average daily gain,and average daily feed intake indices in early weaning ***,RBO protected piglets against LPS-induced jejunal epithelium damage,which was indicated by the increases in villus height,villus height/crypt depth ratio,and Claudin-1 levels,as well as a decreased level of jejunal epithelium *** also improved the antioxidant ability of LPS-challenged piglets,which was indicated by the elevated concentrations of catalase and superoxide dismutase,and increased total antioxidant capacity,as well as the decreased concentrations of diamine oxidase and malondialdehyde in ***,RBO improved the immune function of LPS-challenged weaned piglets,which was indicated by elevated immunoglobulin A(IgA),IgM,β-defensin-1,and ly

关键词： Rice bran oil Gut microbiota Weaned piglets Oxidative stress Lipopolysaccharide

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Real-time Prediction Model of Amount of Manure in Winter pig Pen Based on Backpropagation Neural Network

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Journal of Northeast Agricultural University(English Edition) 2022年第4期29卷 77-90页

作者： Hu Zhen-nan Sun Hong-min Li Xiao-ming Dai Bai-sheng Gao Yue Wang Yu-han College of Electrical and Information Northeast Agricultural UniversityHarbin 150030China Ministry of Agriculture and Rural Affairs Key Laboratory of Pig Breeding Facilities EngineeringHarbin 150030China

The automatic control of cleaning need to be based on the total amount of manure in the house. Therefore, this article established a prediction model for the total amount of manure in a pig house and took the number of pigs in the house, age, feed intake,feeding time, the time when the ammonia concentration increased the fastest and the daily fixed cleaning time as variable factors for modelling, so that the model could obtain the current manure output according to the real-time input of time. A Backpropagation(BP) neural network was used for training. The cross-validation method was used to select the best hyperparameters, and the genetic algorithm(GA), particle swarm optimization(PSO) algorithm and mind evolutionary algorithm(MEA) were selected to optimize the initial network weights. The results showed that the model could predict the amount of manure in real-time according to the model input. After the cross-validation method determined the hyperparameters, the GA, PSO and MEA were used to optimize the manure prediction model. The GA had the best average performance.

关键词： manure amount BP neural network weight optimization algorithm cross-validation

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