检索结果-南通市图书馆

Heptamethine cyanines in bioorthogonal chemistry

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第2期35卷 131-137页

作者： Yuanyuan Liao Yuting Liang Yurou Huang Xiaoyan Zeng Tian He Jun Yin National key laboratory of Green pesticide International Joint Research Center for Intelligent Biosensor Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan 430079China

Due to their excellent fluorescence properties and biological function,cyanine dyes have been widely applied in biological imaging.Heptamethine cyanine(Cy7)dyes,as a type of classic near-infrared(NIR)fluorescent dyes,are considered as one of the effective fluorescent tools in the living organisms due to their good biocompatibility and very low background interference.Bioorthogonal reactions performed in living cells and tissues have developed by leaps and bounds in recent years.The NIR fluorescent labeling technique involving cyanine has attracted widespread attention.This review summarizes their recent application in the field of bioorthogonal imaging,mainly concluding Cy7-type dyes,labeling strategy,bioimaging application,etc.We expect this work can provide some helps for the studies of NIR bioorthogonal reaction in vivo.

关键词： Fluorescent probes Heptamethine cyanines Bioorthogonal reaction Imaging Cell

Molecular identification and enzymatic properties of laccase2 from the diamondback moth Plutella xylostella (Lepidoptera:Plutellidae)

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第10期17卷 2310-2319页

作者： LIU Zhen-gang WANG Huan-huan XUE Chao-bin key laboratory of pesticide toxicology and application technique College of Plant Protection Shandong Agricultural University

Laccase（EC 1.***.3.2）is known to oxidize various aromatic and nonaromatic compounds via a radical-catalyzed reaction,which generally includes two types of laccase,Lac1 and Lac2.Lac1 oxidizes toxic compounds in the diet,and Lac2 is known to play an important role in melanizing the insect exoskeleton.In this study,we cloned and sequenced the cDNA of the diamondback moth,Plutella xylostella Lac2（PxLac2）,from the third instar larvae using polymerase chain reaction（PCR）and rapid amplification of cDNA ends techniques.The results showed that the full-length PxLac2 cDNA was 1 944 bp long and had an open reading frame of 1 794 bp.PxLac2 encoded a protein with 597 amino acids and had a molecular weight of 66.09 kDa.Moreover,we determined the expression levels of PxLac2 in different stages by quantitative PCR（qPCR）.The results indicated that PxLac2 was expressed differently in different stages.We observed the highest expression level in pupae and the lowest expression level in fourth instar larvae.We also investigated the enzymatic properties of laccase,which had optimal activity at pH 3.0 and at 35°C.Under these optimal conditions,laccase had a Michaelis constant（Km）of 0.97 mmol L-1,maximal reaction speed（Vm）of 56.82 U mL-1,and activation energy（Ea）of 17.36 kJ mol-1 to oxidize2,2＇-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid ammonium salt）.Type II copper enhanced laccase activity below 0.8mmol L-1 and reduced enzyme activity above 0.8 mmol L-1 with an IC50 concentration of 1.26 mmol L-1.This study provides insights into the biological function of laccase.

关键词： laccase melanization in vivo expression enzymatic properties Plutella xylostella

Overexpression of a Glycosyltransferase Gene from a Metabolically Poly-Resistant Beckmannia syzigachne Population Alters Growth and Confers Herbicide Resistance to Brachypodium distachyon

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2022年第4期91卷 761-772页

作者： Yizhao Huang Nannan Li Deya Wang Jian Du Weitang Liu Jinxin Wang Wei Li College of Food Science and Pharmaceutical Engineering Zaozhuang UniversityZaozhuang277160China Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing100093China key laboratory of pesticide toxicology and application technique College of Plant ProtectionShandong Agricultural UniversityTai’an271018China College of Life Sciences Zaozhuang UniversityZaozhuang277160China

Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China.The B.syzigachne(AH-02)population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl.To investigate the function of GT73C1 in this population,the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction,and the sequence was 100%consistent with the transcriptome data.Its phylogenetic tree was displayed and annotated using FigTree v1.4.4.The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants.Furthermore,the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl,which was consistent with the findings from the whole plant bioassay.These results indicate that GT73C1 is closely related to the metabolic resistance of B.distachyon.

关键词： Beckmannia syzigachne GT73C1 gene phylogenetic analyses Brachypodium distachyon genetic transformation

Antifungal,Insecticidal and Herbicidal Properties of Volatile Components from Paenibacillus polymyxa Strain BMP-11

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2011年第5期10卷 728-736页

作者： ZHAO Li-jing YANG Xiao-nan LI Xiang-ying MU Wei LIU Feng key laboratory of pesticide toxicology ＆ application technique College of Plant Protection Shandong Agricultural University Tai＇an271018 P.R.China

The volatiles produced by Paenibacillus polymyxa strain BMP-11 which was isolated from the rhizosphere soil of cucumber in a greenhouse were analyzed by SPME-GC-MS.Based on the preliminary test,three kinds of purchased commercial products 1-octen-3-ol,benzothiazole and citronellol were chosen to give further assessment of their bioactivity.Antifungal bioassays in sealed dishes revealed that those three compounds strongly inhibited the mycelia growth of the eight pathogens at a low treatment dosage and induced the mycelial morphological abnormalities.During the experiment,we even found that citronellol completely prevented the pigment production of the tested fungus,Fusarium oxysporum,however,1-octen-3-ol and benzothiazole had slight effect.The germination was inhibited to different degrees when spores of Botrytis cinerea exposed to these compounds for 24 h in water agar plate.Furthermore,fumigation results showed that 1-octen-3-ol and benzothiazole had strong toxicity against Tribolium castaneum,LC50 was 16.76 and 3.50 mg L-1,respectively.The fumigation activity was also found similar to the positive control,1,3-dichloropropene （LC50 =10.13 mg L-1）.Results of herbicidal assays showed that tested compounds had inhibitory effects on the seed germination and seedling growth of Amaranthus retroflexus,Echinochloa crusgalli and Chenopodium album.Findings of the present study suggested that the antifungal,insecticidal and herbicidal properties of the components were contained in volatiles.These agents or even their derivatives may have a potential to be used as fungicide,insecticide as well as herbicide.

关键词： volatiles antifungal activity insecticidal activity herbicidal activity

Inhibitory Kinetics of p-Substituted Benzaldehydes on Polyphenol Oxidase from the Fifth Instar of Pieris Rapae L.

在线全文

学校读者我要写书评

暂无评论

Tsinghua Science and Technology 2007年第4期12卷 400-404页

作者：王勤柯莉娜薛超彬罗万春陈清西 key laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University key laboratory of pesticide toxicology and application technique College of Plant Protection Shandong Agriculture University

Polyphenol oxidase （PPO） is the enzyme responsible for enzymatic browning during the growth of insects. It is also involved in defense reactions and is related with immunities in insects. PPO a metalloenzyme oxidase, catalyzes the oxidation of o-diphenol to o-quinone. The present paper describes the effects of benzaldehyde and its p-substituted derivatives on the activity of PPO from the fifth instar of Pieris rapae L. PPO from the fifth instar of Pieris rapae L. was purified using ammonium sulfate fractionation and chromatography on Sephadex G-100. The enzyme kinetics was characterized using L-3,4-dihydroxyphenylalanine （L-DOPA） as substrate. The results show that benzaldehyde, phydroxybenzaldehyde, p-chlorobenzaldehyde, and p-cyanobenzaldehyde can inhibit the PPO activity for the oxidation of L-DOPA. The inhibitor concentration leading to 50% activity lost, IC50, was estimated to be 5.90, 5.62, 2.83, and 2.91 mmol/L for the four tested inhibitors, respectively. Kinetic analyses show that the inhibitory effects of these compounds are reversible. Benzaldehyde, phydroxybenzaldehyde, and p-chlorobenzaldehyde are noncompetitive inhibitors while p-cyanobenzaldehyde is a mixed-type inhibitor. The inhibition constants were determined for all four inhibitors. p-chlorobenzaldehyde and p-cyanobenzaldehyde were more potent inhibitors than the other compounds. These results provide a basis for developing PPO inhibition-based pesticides.

关键词： Pieris rapae L. the fifth instar polyphenol oxidase benzaldehyde p-substituted derivatives inhibition inhibitory type kinetics mechanism

Photosensitivity and a precise combination of size-dependent lambda-cyhalothrin microcapsules synergistically generate better insecticidal efficacy

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第5期22卷 1477-1488页

作者： GAO Yue LUO Jian SUN Yue ZHANG Hua-wei ZHANG Da-xia LIU Feng MU Wei LI Bei-xing key laboratory of pesticide toxicology&application techniques Shandong Agricultural UniversityTai’an 271018P.R.China College of Plant Protection Shandong Agricultural UniversityTai’an 271018P.R.China Heilongjiang Forest Industry Agrochemical Co. Ltd.Harbin 150324P.R.China

In this study, lambda-cyhalothrin(LC) loaded polyurea microcapsules(MCs) with different particle sizes were fabricated.All of the MCs showed varying degrees of physical collapse, which was more obvious among those with smaller particle sizes. MCs with particle sizes of 1.38 μm(MC-S), 5.13 μm(MC-M) and 10.05 μm(MC-L) had shell thicknesses of 39.6,50.3 and 150.1 nm, respectively. MCs with smaller particles tended to have significantly faster release profiles, and the MC-S group had much higher bioactivity against Agrotis ipsilon and better foliar affinity on the peanut leaves(indicated by rainfastness) than MC-M and MC-L. All of the MCs exhibited light-enhanced release profiles and had much slower degradation compared with the emulsifiable concentrate(EC) group, among which MC-L had the slowest degradation.To generate MCs with both favorable quick efficacy and long-lasting efficacy, binary mixtures of MC-S, MC-M and MC-L were produced by mixing them in pairs at ratios of 2:1, 1:1 and 1:2. The mixture of MC-S:MC-L at 1:2 showed the best comprehensive efficacy in the peanut foliar spray scenario among the nine tested combinations, and its effective duration was three times longer than that of EC. Overall, the precise combination of MCs with different particle sizes can regulate the efficacy of pesticide control and serve as a strategy for the better utilization of pesticides.

关键词： polyurea microcapsules size control combination strategy pest control initial and long-term efficacy

Antagonistic Activities of Volatiles from Four Strains of Bacillus spp. and Paenibacillus spp. Against Soil-Borne Plant Pathogens

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第9期7卷 1104-1114页

作者： LIU Wei-wei MU Wei ZHU Bing-yu DU You-chen LIU Feng key laboratory of pesticide toxicology ＆ application technique College of Plant Protection Shandong Agricultural University Tai＇an 271018. P.R.China

The four effective antagonistic Bacillus strains, isolated from the rhizosphere soil of cucumber in a greenhouse, produced antifungal volatiles. These volatiles strongly inhibited the growth of the most tested pathogenic fungi with wide host plants, induced the mycelial morphological abnormalities, and decreased the sclerotoid production of Sclerotinia sclerotiorum in sealed plates. Spores of Botrytis cinerea exposed to these volatiles for 24-48 h in cavity slides cracked and the sporaceous inclusion became brown and effused to the suspension. An interesting phenomenon observed was that all the bacterial volatiles exhibited intense inhibitory activities against the pigment formation of tested pathogenic fungi, including Ascochyta citrullina, Alternaria solani, Alternaria brassicae, and so on. Interactions mediated by microbial volatiles could be widespread in soils, and volatiles may play an important role in reducing disease levels. A phylogenetic analysis based on 16S rDNA sequence placed the four bacteria in three species Paenibacillus polymyxa （BMP-11）, Bacillus subtilis （BL02）, and Bacillus pumilus （BSH-4 and ZB 13）. Through headspace sampling and GC-MS analysis, a rich profile was found from B. subtilis and overlapping volatile patterns could be found among the different species. Studies are under the way to find the possible action mechanisms and to seek the effective application of bacterial volatiles in greenhouse.

关键词： Bacillus spp. Paenibacillus spp. antifungal volatiles biocontrol headspace-GC/MS

Effects of Tebufenozide on the Biological Characteristics of Beet Armyworm (Spodoptera exigua Hübner) and Its Resistance Selection

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第10期7卷 1222-1227页

作者： LIU Wei-wei MU Wei ZHU Bing-yu LIU Feng key laboratory of pesticide toxicology ＆ application technique College of Plant Protection Shandong Agricultural University Tai＇an271018 P.R.China

In this article, the selection of tebufenozide to beet armyworm （Spodoptera exigua Htibner） was studied by the treatments to alternative generations＇ 3rd-instar larvae with LC50 dose and to continuous generations＇ larvae with LC10 dose; the effects of tebufenozide on the biological characteristics of current and subsequent generations were examined by the treatments to 3rd-instar larvae and egg pods in different concentrations. After treatments with LC50 dose till F11, the toxicity of tebufenozide to beet armyworm had no significant change, whereas the pupation rate, pupal weight, and fecundity were reduced markedly. After treatments with LC10 dose till Fl9, the beet armyworm only developed 3.52-fold resistance, and the main biological characteristics were nearly accordant in each generation. The livability was reduced 72 h later after treatments to 3rd-instar larvae, respectively in 2.5-40 μg mL^-1, and larval duration, pupation rate, and pupal weight changed considerably with the increase in concentrations. The fecundity, larval livability, larval weight and pupal weight of subsequent generations were reduced as the dose increased over 10 μg mL^-1. The hatching rate of egg pods did not differ with that of the controls obviously after treatment in 10-300 μg mL^-1. But the larval livability, larval weight and pupal weight were reduced when eggs were exposed to 50 μg mL^-1 dose or more. The results indicated that tebufenozide had low resistance risk to the current and subsequent generations of beet armyworm even if tebufenozide had significant effects on the biological characteristics of this insect.

关键词： tebufenozide beet armyworm growth and development resistance selection

Phlorizin alleviates deltamethrin-induced oxidative stress in brine shrimp Artemia

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2024年第1期42卷 163-173页

作者： Dandan MA Qingli ZHOU Liying SUI Qingbin GUO Huanhuan LIU Honghe LIANG Zhenjing LI Zhongna SANG School of Food Science and Engineering Tianjin University of Science and TechnologyTianjin 300457China Asian Regional Artemia Reference Center Key Laboratory of Marine Resource Chemistry and Food Technology(TUST)Ministry of EducationTianjin University of Science and TechnologyTianjin 300457China Subtropical Crops Research Institute of Guangxi Zhuang Autonomous Region Nanning 530001China Department of Nutrition and Food Hygiene School of Public HealthTianjin Medical UniversityTianjin 300070China

Deltamethrin(DEL),a commonly used pyrethroid pesticide,results in higher reactive oxygen species(ROS)levels in aquatic animals,which consequently unbalance the redox state.Phlorizin(PHL)is a flavonoid and a natural product promising to prevent or reduce pesticide-induced oxidative stress.Artemia is a micro-crustacean widely used in marine hatcheries and an experimental aquatic organism for environmental toxicology research.This research aimed to evaluate the toxicity of DEL on Artemia and the antioxidative effect of PHL against the toxicity.Results show that 0.08-mg/mL PHL exerted its antioxidative effects on hatching percentage of the cysts in 24-h incubation and on body length and survival rate of Artemia in 12-d culture.After 12-d culture,12-,24-,and 36-h DEL exposure showed significant drops in SOD,CAT,and GSH-Px enzyme activities,and significant increases in ROS and malondialdehyde(MDA)levels in Artemia(Papplication improved the enzyme activities and decreased the ROS and MDA levels(Ppesticide-induced toxicity in aquatic animals.

关键词： Artemia deltamethrin phlorizin oxidative stress