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Research and Application of the novel Deep Plugging Method in the Oilfield

Journal of Materials Science and Chemical Engineering 2019年第1期7卷 10-19页

作者： Juan Zhao Hongfu Fan Long Liu Yi Jia School of Energy resources China University of Geosciences (Beijing) Beijing China key laboratory of Marine Reservoir Evolution and Hydrocarbon Enrichment Mechanism Ministry of Education Beijing China Beijing key laboratory of Unconventional Natural Gas Geological Evaluation and Development Engineering Beijing China

In long-term water drive reservoir, small dose and short radius profile control cannot meet the needs, so deep profile control and flooding are needed. During the placement and processing of conventional deep profile control and flooding agent, the zone classification formed by the changes of pressure field and fluid field are not taken into account. In order to better develop these reservoirs, we proposed a novel deep profile control method, that is, the iso-pressure drop gradient progressive deep profile control method. The key features of the method include: 1) the method took into account the reservoir pressure distribution;2) it proposed a novel standard to divide the orders of zone;3) the method has been successfully applied in 4 wells in China Oilfield. The method divided the formation into near wellbore zone, well-far zone and deep zone according to the drawdown curve. As each zone of the pressure gradient is different and therefore require different intensity of slug. Then design the agent slugs according to iso-pressure drop gradient rule, and the breakthrough pressure gradient of agent is equal to the formation pressure gradient, and it achieved the fluid diversion of whole course by combining the different intensity of blocking agent. The method was applied successfully in 2 wells in China Oilfield from May to November in 2008. The method can smartly improve the sweep efficiency, and field test shows that it can play a very good efficiency of reducing water and increasing oil production. This method is becoming more of a concern in the oilfield develop.

关键词： Deep Profile Control Heterogeneity Pressure Distribution Iso-Pressure Drop Gradient

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Removal of roosters alters the domestic phenotype and microbial and genetic profile of hens

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Science China(Life Sciences) 2021年第11期64卷 1964-1976页

作者： Hai Xiang Siyu Chen Hui Zhang Xu Zhu Dan Wang Huagui Liu Jikun Wang Tao Yin Langqing Liu Minghua Kong Jian Zhang Hua Li Simon Turner Xingbo Zhao Guangdong Provincial key laboratory of Animal Molecular Design and Precise Breeding School of Life Science and EngineeringFoshan UniversityFoshan 528225China National Engineering laboratory for Animal Breeding Key Laboratory of Animal GeneticsBreeding and ReproductionMinistry of AgricultureCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Institute of Animal Husbandry and Veterinary Medicine Beijing Academy of Agricultural and Forestry SciencesBeijing 100097China key laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Ministry of EducationInstitute of Tibetan Plateau ResearchSouthwest Minzu UniversityChengdu 610041China Animal and Veterinary Sciences Research Group Scotland’s Rural CollegeEdinburghEH93JGUK

Hens are raised apart from roosters in modern poultry production, a substantial change from their natural social structure. We compared productivity, injuries, behavior, physiology, microbiome and transcriptome of hens housed with(R+) or without(R–)roosters to quantify the effects of this change in social structure. Hens were raised free-range from 70 to 280 days when 30 birds per treatment were assigned to battery cages until Day 315(R+C vs. R–C), while 30 birds per treatment remained in free-range pens(R+F vs. R–F). Response to a novel environment and object, behavioral time budgets, cecum microbiome, blood composition and transcriptomic sequencing of thigh muscle and spleen were analyzed. Hens housed without roosters showed better survival, consumed less food, produced more eggs and had better feed conversion. R+F hens clustered around the rooster and were less mobile in the novel environment and object tests. R+F hens displayed the richest microbiome, and the presence of roosters resulted in differentially expressed genes related to muscle development, cellular processes, environmental information processing and immune function. Removing roosters from housed hens intensified desirable characteristics favored by domestication probably operating by deprivation of mating behavior and reduced fear, along with altered microbial and genetic function.

关键词： chicken domestic phenotype fear gut microbiome rooster transcriptome

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Low-field NMR inversion based on low-rank and sparsity restraint of relaxation spectra

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Petroleum Science 2022年第6期19卷 2741-2756页

作者： Si-Hui Luo Li-Zhi Xiao Yan Jin Jiang-Feng Guo Xiao-Bo Qu Zhang-Ren Tu Gang Luo Can Liang College of Artificial Intelligence and College of Petroleum Engineering China University of PetroleumBeijing102249China State key laboratory of Petroleum resources and Prospecting China University of PetroleumBeijing102249China School of Electronic Science and Engineering(National Model Microelectronics College) Xiamen UniversityFujian361005China School of Electronic Science and Engineering National Model Microelectronics College Xiamen University Changzhou Institute of Technology ChangzhouJiangsu213000China

In this paper,we proposed a novel method for low-field nuclear magnetic resonance(NMR)inversion based on low-rank and sparsity restraint(LRSR)of relaxation spectra,with which high quality construction is made possible for one-and two-dimensional low-field and low signal to noise ratio NMR *** this method,the low-rank and sparsity restraints are introduced into the objective function instead of the smoothing *** low-rank features in relaxation spectra are extracted to ensure the local characteristics and morphology of *** sparsity and residual term are contributed to the resolution and precision of spectra,with the elimination of the redundant relaxation *** process of the objective function is designed with alternating direction method of multiples,in which the objective function is decomposed into three subproblems to be independently *** optimum solution can be obtained by alternating iteration and updating *** first,numerical simulations are conducted on synthetic echo data with different signal-to-noise ratios,to optimize the desirable regularization parameters and verify the feasibility and effectiveness of proposed ***,NMR experiments on solutions and artificial sandstone samples are conducted and analyzed,which validates the robustness and reliability of the proposed *** results from simulations and experiments have demonstrated that the suggested method has unique advantages for improving the resolution of relaxation spectra and enhancing the ability of fluid quantitative identification.

关键词： Low-field NMR Inversion method Low-rank and sparsity restraint Relaxation spectra Data processing

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Biochemical characterization of a novel GH19 chitinase from Streptomyces alfalfae ACCC40021

Biochemical characterization of a novel GH19 chitinase from ...

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第十一届中国酶工程学术研讨会

作者：谷天燕 Yukun Zhang Lingcong Li Lingling Wang Xiaonan Liu Chenyin Lv Jingang Gu Guogang Zhao College of Life Sciences Hebei Agricultural Univeristy key laboratory of Microbial resources Ministry of Agriculture Institute of Agricultural resources and Regional Planning Chinese Academy of Agricultural Sciences

Chitin,a linear P-1,4-linked polymer of N-acetylglucosamine（G1 cNAc）,is one of the most abundant renewable material on *** exists in the crustacean and insect shells,as well as fungal cell ***（EC3.***.1.14） play a vital role not only in manufacturing chitooligosaccarides（CHOS） for pharmacology and food industry,but in biological control of phytopathogens by degrading the chitin polymer in the cell walls of fungus.A novel Chitinase gene saChiB from Streptomyces alfalfae ACCC40021,belonging to glycoside hydrolase family 19（GH19）,was cloned in pET30 a（+）,and expressed successfully in Escherichia coli BL21（DE3）.The recombinant saChiB was purified by Ni-NTA affinity *** purified enzyme activity was224.85 U/*** molecular mass was approximately 34 KDa by *** displayed maximal activity for hydrolysis of colloidal chitin at pH8.0 and 50℃.The thermostability of recombinant enzyme showed it remained about 50%residual activity after 2 h incubated at 70 ℃.The Km value for colloidal chitin was determined to be9.42 mg/*** exhibited broad substrate specificity for colloidal chitin,glycol chitin,a-chitin,β-（1,3）-glucan and ***,it showed relative lysozyme *** assay revealed that saChiB strongly inhibited the growth of fungal pathogens such as Botrytis cinerea,Fusarium oxysporum,Sclerotinia sclerrotiorum,Trichoderma virida,Rhizoctonia *** results indicate that saChiB has the potential to be utilized as bio-control agent against plant pathogen fungus.

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Determination of mechanical properties of Zanthoxylum armatum using the discrete element method

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food Quality and Safety 2022年第4期6卷 709-719页

作者： Jian Zhang Hong Zhou Fan Luo Long Wan Chengsong Li Lihong Wang College of Engineering and Technology Southwest UniversityChongqingChina key laboratory of Agricultural Equipment in Hilly and Mountainous Areas ChongqingChina College of Plant Protection Southwest UniversityChongqingChina

Using the discrete element method to investigate the behavior of particles is a crucial strategy in the research and development of novel *** pepper(Zanthoxylum armatum)is a globally renewable plant-derived medicinal and food homologous commodity with a wide range of uses and great demand,but the mechanical properties needed to develop its processing equipment are ***,this case study aimed to systematically explore the necessary input parameters to model green pepper,and to provide new insights for the guidance of future industrial applications *** the basis of the experimental measured physical properties,the contact properties of green pepper on zinc-coated steel were first calibrated and then used to determine the contact properties between *** differences between the experimental and simulation results were analyzed for selection and verification of the contact properties *** analysis confirmed that the co-efficient of restitution,coefficient of static friction and coefficient of rolling friction for contact between the particle and zinc-coated steel have values of 0.392,0.650,and 0.168,and those coefficients for particle-to-particle contact have values of 0.199,0.710,and 0.184,*** in this work may contribute to the research and development of production equipment for green pepper.

关键词： discrete element method green pepper physical properties contact properties difference analysis

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Advances in Biosensing and Environmental Monitoring Based on Electrospun Nanofbers

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Advanced Fiber Materials 2022年第3期4卷 404-435页

作者： Shixiong Kang Kun Zhao Deng‑Guang Yu Xiaolu Zheng Caoxing Huang School of Materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China Shanghai Engineering Technology Research Center for High-Performance Medical Device Materials University of Shanghai for Science and TechnologyShanghai 200093China Jiangsu Co-Innovation Center of Efcient processing and Utilization of Forest resources College of Chemical EngineeringNanjing Forestry UniversityNanjing 210037China

Electrospun nanofbers(NFs)are directly produced by electrospinning *** are useful in a series of applications such as excellent performance in biosensing and environmental monitoring,due to their large specifc surface area and high *** wide range of materials used provide a solid foundation and core guarantee for electrospun NFs to sense,which are used in a variety of polymers,small molecules,colloidal particles,and *** primarily aims at small biomolecules,biomacromolecules,wearable human motion monitoring,and food safety *** monitoring encompasses the detection of gases,humidity,volatile organic compounds,and monitoring the degradation of heavy metal *** aim to sort out some recent research for electrospun NFs in the sensing area,which may inspire emerging smart sensing devices and bring a novel approach for biomedical development and environmental *** highlight the powerful applications of electrospun NFs in the rapidly growing feld of wearable electronic devices,which may spur the industry’s novel perspectives on the development of ***,we point out some unresolved difculties in the sensing feld for electrospun NFs and propose possible and novel ideas for this development.

关键词： Biosensing Environmental monitoring Sensors Wearables Electrospun nanofbers

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High-quality reference genomes of swallowtail butterflies provide insights into their coloration evolution

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Zoological Research 2022年第3期43卷 367-379页

作者： Jin-Wu He Ru Zhang Jie Yang Zhou Chang Li-Xin Zhu Si-Han Lu Fei-Ang Xie Jun-Lai Mao Zhi-Wei Dong Gui-Chun Liu Ping Hu Yan Dong Wen-Ting Wan Ruo-Ping Zhao Tian-Zhu Xiong Jorge LLeón-Cortés Chu-Yang Mao Wei Zhang Shuai Zhan Jun Li Lei Chen Wen Wang Xue-Yan Li State key laboratory of Genetic resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China School of Ecology and Environment Northwestern Polytechnical UniversityXi’anShaanxi 710072China Department of Biology and food Engineering Chuzhou UniversityChuzhouAnhui 239000China School of Marine Science and Technology Zhejiang Ocean UniversityZhoushanZhejiang 316022China Department of Organismic and Evolutionary Biology Harvard UniversityCambridgeMA 02138USA Ecología para la Conservación de la Fauna Silvestre El Colegio de la Frontera Sur(ECOSUR)Carretera Panamericana y Periférico Sur s/n Barrio María AuxiliadoraSan Cristóbal de Las CasasChiapas CP 29290Mexico State key laboratory of Protein and Plant Gene Research Peking UniversityBeijing 100871China CAS key laboratory of Insect Developmental and Evolutionary Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan 650223China Center for Excellence in Animal Evolution and Genetics KunmingYunnan 650223China

Swallowtail butterflies(Papilionidae)are a historically significant butterfly group due to their colorful wing patterns,extensive morphological diversity,and phylogenetically important position as a sister group to all other butterflies and have been widely studied regarding ecological adaption,phylogeny,genetics,and ***,they contain a unique class of pigments,i.e.,papiliochromes,which contribute to their color diversity and various biological functions such as predator avoidance and mate *** date,however,the genomic and genetic basis of their color diversity and papiliochrome origin in a phylogenetic and evolutionary context remain largely ***,we obtained high-quality reference genomes of 11 swallowtail butterfly species covering all tribes of Papilioninae and Parnassiinae using long-read sequencing *** with previously published butterfly genomes,we obtained robust phylogenetic relationships among tribes,overcoming the challenges of incomplete lineage sorting(ILS)and gene *** genomic analyses indicated that the evolution of Papilionidae-specific conserved non-exonic elements(PSCNEs)and transcription factor binding sites(TFBSs)of patterning and transporter/cofactor genes,together with the rapid evolution of transporters/cofactors,likely promoted the origin and evolution of *** findings not only provide novel insights into the genomic basis of color diversity,especially papiliochrome origin in swallowtail butterflies,but also provide important data resources for exploring the evolution,ecology,and conservation of butterflies.

关键词： High-quality reference genome Swallowtail butterfly tribe Color evolution Papiliochromes

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Identification of marine natural product Pretrichodermamide B as a STAT3 inhibitor for efficient anticancer therapy

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Marine Life Science & Technology 2023年第1期5卷 94-101页

作者： Rui Li Yue Zhou Xinxin Zhang Lujia Yang Jieyu Liu Samantha M.Wightman Ling Lv Zhiqing Liu Chang-Yun Wang Chenyang Zhao School of Medicine and Pharmacy Institute of Evolution&Marine BiodiversityCollege of Food Science and EngineeringOcean University of ChinaQingdao266003China laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao266237China Department of Cancer Biology Lerner Research InstituteCleveland ClinicClevelandOH44195USA

The Janus kinase(JAK)/signal transducer and activator of transcription 3(STAT3)regulates the expression of various critical mediators of cancer and is considered as one of the central communication nodes in cell growth and *** natural products(MNP)represent great resources for discovery of bioactive lead compounds,especially anti-cancer *** the medium-throughput screening of our in-house MNP library,Pretrichodermamide B,an epidithiodiketopiperazine,was identified as a JAK/STAT3 signaling *** studies identified that Pretrichodermamide B directly binds to STAT3,preventing phosphorylation and thus inhibiting JAK/STAT3 ***,it suppressed cancer cell growth,in vitro,at low micromolar concentrations and demonstrated efficacy in vivo by decreasing tumor growth in a xenograft mouse *** addition,it was shown that Pretrichodermamide B was able to induce cell cycle arrest and promote cell *** study demonstrated that Pretrichodermamide B is a novel STAT3 inhibitor,which should be considered for further exploration as a promising anti-cancer therapy.

关键词： Pretrichodermamide B Signal transducer and activator of transcription 3(STAT3) Marine natural products Target protein In vivo anti-cancer efficacy

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Thiocarbonylation of C(sp^(3))-H bonds in pyridylamines with CS;: Facile synthesis of pyrido[1,2-a]pyrimidine-4-thiones

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Chinese Chemical Letters 2021年第12期32卷 4015-4018页

作者： Xiao-Yu Zhou Xiang-Yu Li Zhen Zhang Da-Gang Yu key laboratory of Green Chemistry&Technology of Ministry of Education College of ChemistrySichuan UniversityChengdu 610064China key laboratory of Coarse Cereal processing of Ministry of Agriculture and Rural Affairs College of Food and Biological EngineeringChengdu UniversityChengdu 610106China

Herein, a facile synthesis of valuable pyrido[1,2-a]pyrimidine-4-thiones is reported via novel thiocarbonylation of C(sp^(3))-H bonds with carbon disulfide(CS_(2)). This reaction features easy availability of substrates,good functional group tolerance, high yields, facile scalability and atom economy. Mechanistic investigations indicate that sulfate anion and sulfuric anhydride anion might be involved in this reaction.

关键词： Pyridinylamines Thiocarbonylation Pyrido[1,2-a]pyrimidine-4-thiones Carbon disulfide Ketoimines

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Biological evaluations of decellularized extracellular matrix collagen microparticles prepared based on plant enzymes and aqueous two-phase method

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Regenerative Biomaterials 2021年第2期8卷 64-73页

作者： YaWen Liu Ching-Cheng Huang YuanYuan Wang Jun Xu GuoDing Wang XinPeng Bai Engineering Research Center of Utilization of Tropical Polysaccharide resources College of Food Science and TechnologyHainan UniversityHaikouHainan 570000China PARSD Biomedical Material Research Center(PBMRC) ChangzhouJiangsu 213000China Department of Biomedical Engineering Ming-Chuan UniversityTaoyuan 320-33Taiwan

For patients with extensive full-thickness burns who do not have sufficient autologous splitthickness skin for skin grafts,the application of biological skin substitutes may be *** aim of this study was to find an optimal new type method for the production of a biovital skin substitute based on acellular dermal matrix(ADM)and preclinical *** this work,25 methods of ADM production were *** proposed methods are based on the use of the following enzymes:papain,Carica papaya lipase(CPL),and purification using a polymer/salt aqueous two-phase *** obtained ADM samples were characterized via scanning electron microscopy(SEM),porosity measurement and water vapor transmission *** showed that the collagen bundles of ADM microparticles were intact and *** differential scanning calorimetry(DSC),thermo gravimetric analysis(TGA)and biocompatibility tests,the results indicated that the proportion of papain and CPL was the same and 5 h processing time are the optimum conditions for ADM preparation and the material showed good *** results suggested that the potential of developing this kind of decellularization process to manufacture ADM scaffolds for clinical application.

关键词： acellular dermal matrix papain Carica papaya lipase aqueous two-phase extraction

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