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Ectopic expression of VvFUS3,B3-domain transcription factor,in tomato influences seed development via affecting endoreduplication and hormones

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Horticultural Plant Journal 2022年第3期8卷 351-360页

作者： Bilal Ahmad Songlin Zhang Jin Yao Shengyue Chai Vivek Yadav Habib-ur-Rehman Athar Mati Ur Rahman Li Wang Xiping Wang State key laboratory of Crop Stress Biology in Arid Areas College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China key laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China Ministry of AgricultureNorthwest A&F UniversityYanglingShaanxi 712100China Institute of Pure and Applied Biology Bahauddin Zakariya UniversityMultan 60800Pakistan College of food Engineering and Nutritional Science Shaanxi Normal UniversityXi’an 710119China College of Horticulture Hebei Agricultural UniversityBaodingHebei 071000China

FUSCA3(FUS3)is a member of B3-domain transcription factor family and master regulator of seed *** has potential roles in hormone biosynthesis and signaling pathways and therefore plays diverse roles in plant life cycle,especially in seed germination,dormancy,embryo formation,seed and fruit development,and ***,there is limited information about its functions in seed and fruit development of *** this study,we expressed VvFUS3 in tomato for its functional *** of VvFUS3 in tomato led to a reduction in seed number and seed weight without affecting the fruit *** analysis found that both cell expansion and cell division in transgenic seed and fruit pericarp have been ***,there were no obvious differences in pollen size,shape,and viability,suggesting that VvFUS3 affects seed development but not the pollen ***,the expression of several genes with presumed roles in seed development and hormone signaling pathways was also influenced by *** results suggest that VvFUS3 is involved in hormonal signaling pathways that regulate seed number and *** conclusion,our study provides novel preliminary information about the pivotal roles of VvFUS3 in seed and fruit development and these findings can potentially serve as a reference for molecular breeding of seedless grapes.

关键词： Vitis vinifera L. ABI3 B3 transcription factor Seed number Endoreduplication Hormone

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一种新型药剂方案浮选分离黄铁矿和铜金硫化矿的案例研究:第一性原理计算的视角

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Journal of Central South University 2023年第5期30卷 1552-1568页

作者：罗远家孙伟欧乐明韩海生江锋 Stephen POOLEY 陈建华彭建 School of Minerals processing and Bioengineering Central South UniversityChangsha 410083China School of resources Environment and MaterialsGuangxi UniversityNanning 530004China

由于石灰的大量使用容易导致堵管、环境污染、贵金属回收困难等问题,以石灰(CaO)为抑制剂、黄药(如丁基黄药钠)(BX)为捕收剂的传统方案的使用越来越受到限制,无石灰浮选工艺越来越被提倡。本研究创新性地提出了以Z200为捕收剂、焦没食子... 详细信息

由于石灰的大量使用容易导致堵管、环境污染、贵金属回收困难等问题,以石灰(CaO)为抑制剂、黄药(如丁基黄药钠)(BX)为捕收剂的传统方案的使用越来越受到限制,无石灰浮选工艺越来越被提倡。本研究创新性地提出了以Z200为捕收剂、焦没食子酸(PGA)为抑制剂的新工艺,并应用于湖北某铜金硫化矿与黄铁矿的分离。在实验室试验中,该试剂方案得到了比常规浮选工艺更好的铜金硫化矿与黄铁矿分离指标。利用基于密度泛函理论(DFT)的第一性原理计算进一步研究新药剂方案分离机理。DFT模拟结果表明,PGA对黄铁矿的抑制作用比黄铜矿强,而Z200对黄铜矿的捕收能力比黄铁矿强,这在分子水平上解释了新药剂方案的效果。这一新型环保药剂方案在矿产资源利用中具有很大的应用潜力。

关键词：新工艺铜金硫化矿黄铁矿分离 DFT模拟

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How many probe vehicles are enough for identifying traffic congestion？--a study from a streaming data perspective

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Frontiers of Earth Science 2013年第1期7卷 34-42页

作者： Handong WANG Yang YUE Qingquan LI State key laboratory of Information Engineering in Surveying Mapping and Remote Sensing Wuhan University Wuhan 430079 China Engineering Research Center for Smart Acquisition and Applications of Spatiotemporal Data Ministry of Education Wuhan 430079 China Changjiang Institute of Survey Planning Design and Research Changjiang Water Resources Commission Wuhan 430010 China

Many studies have been carried out using vehicle trajectory to analyze traffic conditions, for instance, identifying traffic congestion. However, there is a lack of a systematic study on the appropriate number of probe vehicles and their sampling interval in order to identify traffic congestion accurately. Moreover, most of related studies ignore the streaming feature of trajectory data. This paper first represents a novel method of identifying traffic congestion considering the stream feature of vehicle trajectories. Instead of processing the whole data stream, a series of snapshots are extracted. Congested road segments can be identified by analyzing the clusters＇ evolution among a series of adjacent snapshots. We then calculated a series of parameters and their corresponding congestion identification accuracy. The results have implications for related probe vehicle deployment and traffic analysis; for example, when 5% of probe vehicles are available, 85% identification accuracy can be reached if the sampling time interval is 10 s.

关键词： vehicle streaming data traffic trajectory data floating car data congestion

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Antibacterial activities of a novel Cu-bearing high-entropy alloy against multi-drug-resistant Acinetobacter baumannii and Staphylococcus aureus

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Rare Metals 2022年第2期41卷 570-579页

作者： En-Ze Zhou Guang-Yu Ren Yun-Ling Sun Yong-Qiang Fan Yi Yang Ming-Yue Sun Da-Ke Xu Yi-Ping Lu Jian-Jun Wang Hua-Bing Li Fu-Hui Wang Shenyang National laboratory for Materials Science Key Laboratory for Anisotrotropy and Texture of Materials(Ministry of Education)School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China key laboratory of Solidification Control and Digital Preparation Technology(Liaoning Province) School of Materials Science and EngineeringDalian University of TechnologyDalian 116024China General Hospital of Northern Theater Command Shenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus(MRSA)are two prevalent pathogens and have developed high resistant to most ***,it is a pressing need to develop a new method to inhibit the spread of drug-resistant *** containing high-entropy alloy(HEA,Al0.4CoCr-CuFeNi)is a new kind of alloy material,which shows extensive antibacterial activity and mechanical properties in our previous *** study further develops another HEA(CoCrCuFeNi)and evaluates its resistance against gram-negative *** and Gram-positive *** antibacterial tests show that the antibacterial rate of the HEA toward both bacteria reached nearly 99%,far better than the traditional copper-bearing 304 stainless steel(304 Cu-SS).The biofilm observation shows that the HEA could not only kill the planktonic bacteria,but also effectively inhibit the formation of *** data demonstrate that CoCrCuFeNi HEA possesses effective antibacterial and antibiofilm activities,making it a potential candidate for using in hospital,food industry,and domestic kitchens.

关键词： High-entropy alloy Acinetobacter baumannii Methicillin-resistant Staphylococcus aureus(MRSA) Antibacterial activity Antibiofilm activity

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novel Tuning Buttons in Tomato Fruit Ripening:miRNAs and siRNAs

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Agricultural Science & Technology 2016年第7期17卷 1541-1545页

作者： Jinhua ZUO Qing WANG Zheng JU Dongyan CAO Benzhong ZHU Yunbo LUO Lipu GAO National Engineering Research Center for Vegetables Beijing 100097 China Beijing key laboratory of Fruits and Vegetable Storage and processing Beijing 100097 China College of food Science and Nutritional Engineering China Agricultural University Beijing 100083 China

Tomato has emerged as an emblematic model plants for fleshy fruit research and tomato fruit ripening is a complex and highly coordinated developmental *** many physiology and biochemical processes associated with tomato fruit ripening require changes in the expression of hundreds to thousands of *** expression is regulated by transcriptional and post-transcriptional pathways,one of the recently discovered mechanisms in plants was small RNAs mediated gene silencing at post-transcriptional(PTGS) ***,several mi RNAs and endogenous si RNAs were revealed to be involved in the fruit ripening process which opened a new avenue in the field of fleshy fruit *** review compiled the most recent advances made in deciphering the regulation functions of mi RNAs and si RNAs in tomato fruit *** also emphasized the new perspectives now possible in the small RNAs regulation research in tomato fruit ripening and senescence.

关键词： tomato siRNAs hundreds developmental emphasized Ethylene biochemical endogenous coordinated miRNAs

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A label-free ultrasensitive fluorescence detection of viable Salmonella enteritidis by NMM/G-quadruplex DNA based on enzyme-induced cascade two-stage toehold strand-displacement

A label-free ultrasensitive fluorescence detection of viable...

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第七届中国畜牧科技论坛

作者： Zhang peng Liu hui Ma suzhen Men shuai Li qingzhou Yang xin Wang hongning College of Life Sciences Animal Disease Prevention and Food Safety Key Laboratory of Sichuan ProvinceSichuan UniversityKey Laboratory of Bio-Resources and Eco-Environment of Ministry of Education"985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern

The harm of Salmonella enteritidis(***) to public health mainly by contaminating fresh food and water emphasizes the urgent need for rapid detection techniques to help control the spread of the *** this assay,an newly designed capture probe complex that contained specific ***-aptamer and hybridized signal target sequence was used for viable *** recognition *** the presence of the target ***,single-stranded target sequences were liberated and initiated the replication-cleavage reaction,producing numerous G-quadruplex structures with a linker on the 3’-*** then,the sensing system took innovative advantage of quadratic linker-induced strand-displacement for the first time to release target sequence in succession,leading to the cyclic reuse of the target sequences and cascade signal amplification,thereby achieving the successive production of Gquadruplex *** fluorescent dye,N-Methyl mesoporphyrin IX,binded to these G quadruplex structures and generated significantly enhanced fluorescent signals to achieve highly sensitive detection of *** down to 60 CFU/mL with a linear range from 10 to 10CFU/*** coupling the cascade two-stage target sequences-recyclable toehold strand-displacement with aptamer-based target recognition successfully,it is the first report on a novel non-label,modification-free and DNA extraction-free ultrasensitive fluorescence biosensor for detecting viable *** directly,which can discriminate from dead ***.

关键词： Fluorescence biosensor Label-free Salmonella enteritidis Aptamer Toehold strand-displacement G-quadruplex

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Fish and fish side streams are valuable sources of high-value components

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food Quality and Safety 2019年第4期3卷 209-226页

作者： Anna-Liisa Välimaa Sari Mäkinen Pirjo Mattila Pertti Marnila Anne Pihlanto Maarit Mäki Jaakko Hiidenhovi Natural resources Institute Finland(Luke) Oulu Natural resources Institute Finland(Luke) Jokioinen Natural resources Institute Finland(Luke) Turku

The current practice of fish processing generates increasing quantities of side streams and waste,such as skin,heads,frames,viscera,and fillet cut *** may account for up to 70%of the fish used in industrial ***-value fish catches,and under-utilized fish species comprise another source of side *** side streams have been discarded in the environment leading to environmental problems or they have ended up as low commercial value products,such as feed for fur animals and ***,several studies have shown that fish side streams contain valuable bioactive ingredients and fractions,such as fish oils,proteins and peptides,collagen,gelatin,enzymes,chitin,and *** compounds and fractions may provide the opportunity to develop novel applications in health promoting foods,special feeds,nutraceuticals,pharmaceuticals,and cosmetic *** utilization of side streams and low-value fish would simultaneously improve both the environmental and ecological sustainability of *** review summarizes the current knowledge on fish and fish side streams as sources of high-value components such as peptides with antimicrobial,antioxidative,antihypertensive,and antihyperglycemic properties,proteins such as fish collagen and gelatin,fish enzymes,fish oils and fatty acids,polysaccharides like glucosaminoglycans,chitin and chitosan,vitamin D,and *** technologies for recovering the high-value fractions and potential product applications are ***,safety aspects related to the raw material,technologies,and fractions are considered.

关键词： fish side streams bioactive peptides fish polysaccharides fish oils

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Diversity analysis of acute hepatopancreatic necrosis disease-positive Vibrio parahaemolyticus strains

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Aquaculture and Fisheries 2017年第6期2卷 278-285页

作者： Bo Feng Haiquan Liu Meng Wang Xiaohong Sun Yingjie Pan Yong Zhao College of food Science and Technology Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquatic-Product processing&Preservation Shanghai 201306China laboratory of Quality&Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai) Ministry of AgricultureShanghai 201306China

Acute hepatopancreatic necrosis disease(AHPND),also called early mortality syndrome(EMS),has caused massive economic loss in aquaculture since *** was considered that the disease was caused by a novel type of Vibrio parahaemolyticus which harbors a large *** insecticidal Photorhabdus insect-related(pir)genes were found in the *** this study,81 isolates have been collected from a shrimp farm in Maoming,Guangdong Province,*** isolates were identified using 3 pairs of primer recently made *** the 16S rRNA gene and pir gene were ***-PCR was employed for analysis of strain diversity.12 isolates were amplified by PCR and identified as Vibrio parahaemolyticus by 16S rRNA sequence *** alignments of the pir gene showed few mutations in the 12 strains of *** 12 strains separated into 3 clusters at a cut of 90%similarity by *** virulence gene expression and toxicity profile showed differences between *** study generated comprehensive identification of pathogenic bacteria and microbial molecular resources,which will contribute to future research of AHPND.

关键词： Acute hepatopancreatic necrosis disease Challenge assay Diversity analysis

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Glycoalkaloids and phenolic compounds in three commercial potato cultivars grown in Hebei,China

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food Science and Human Wellness 2018年第2期7卷 156-162页

作者： Cheng-Yu Jin Hong Liu Dan Xu Fan-Kui Zeng Yu-Ci Zhao Hai Zhang Gang Liu Research&Development Center for Eco-material and Eco-Chemistry Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China CAS key laboratory of Chemistry of Northwestern Plant resources and key laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Zhangjiakou Hongji Agriculture Science and Technology Development Co.Ltd Zhangjiakou 076576China

In order to investigate the food nutrition and safety of potato,the objective of the present study is to quantify glycoalkaloids(-chaconine and-solanine)and phenolic compounds in three potato cultivars,namely,Russet Burbank,Atlantic,and Shepody,grown in Hebei,China,and these two classes of biologically active compounds in commercial dehydrated potato flakes were also *** total glycoalkaloid levels in whole potatoes ranged from 4.72 mg/kg for Shepody potatoes to 34.45 mg/kg for Russet Burbank *** ratio of-chaconine to-solanine in whole potatoes ranged from 0.41 for Russet Burbank potatoes to 3.61 for Atlantic *** removal rate of total glycoalkaloids in Russet Burbank potatoes during dehydrated potato flake processing is 90.0%.Chlorogenic acid is the dominant phenolic compound in Russet Burbank *** contrast,Atlantic and Shepody potatoes contain considerable caffeic *** loss rate of phenolic compounds in Russet Burbank during dehydrated potato flake processing is 50.47%.

关键词： Glycoalkaloids Phenolic compounds Dehydrated potato flake Potato

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基于油酸钙胶体和矿物表面转化的石灰法浮选机理新见解

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Journal of Central South University 2023年第9期30卷 2983-2992页

作者：孙文娟韩海生孙伟王若林 School of Minerals processing and Bioengineering Central South UniversityChangsha 410083China key laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral resources Central South UniversityChangsha 410083China Hunan International Joint Research Center for Efficient and Clean Utilization of Critical Metal Mineral resources Central South UniversityChangsha 410083China Technology Research Center of Hunan Province for Comprehensive Utilization of Associated Fluorite and Fluorine Chemical Engineering Chenzhou 423037China School of resources and Environmental Engineering Shandong University of TechnologyZibo 255000China

石灰法是最常用的白钨矿浮选工艺之一,可以实现白钨矿、萤石和方解石的浮选分离。石灰水解释放的大量钙离子容易与脂肪酸阴离子形成油酸钙胶体,而石灰法的经典作用机理却忽略了这一点。溶液化学计算表明,油酸钙胶体是石灰法矿浆中的主... 详细信息

石灰法是最常用的白钨矿浮选工艺之一,可以实现白钨矿、萤石和方解石的浮选分离。石灰水解释放的大量钙离子容易与脂肪酸阴离子形成油酸钙胶体,而石灰法的经典作用机理却忽略了这一点。溶液化学计算表明,油酸钙胶体是石灰法矿浆中的主要作用成分。石灰法中,由于表面转化反应增强,萤石表面被碳酸钙覆盖,表面性质与方解石表面相似。石灰法矿浆中油酸钙胶体的粒度为100~600 nm,石灰用量对其粒度和粒度分布有显著影响。油酸钙胶体在白钨矿表面发生选择性吸附,而在萤石表面的吸附因表面转化反应而减弱。油酸钙在白钨矿表面的选择性吸附解释了石灰法的优异选择性,萤石向方解石的表面转化反应进一步增强了石灰法矿浆中的油酸钙选择性。石灰法的新作用机理补充了油酸钙在浮选中的作用,进一步完善了之前的经典作用机理。

关键词：白钨矿石灰法油酸钙表面转化萤石方解石

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