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Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory

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Journal of oceanology and Limnology 2022年第3期40卷 1027-1038页

作者： Li SUN Peike GAO Yu LI Chao WANG Ning DING Junfeng CHEN Yuhao SONG Chunchen LIU Lun SONG Renjun WANG College of Life sciences Qufu Normal UniversityQufu 273165China key laboratory of marine biological resources and ecology Liaoning Ocean and Fisheries Science Research InstituteDalian 116023China

Algicidal bacteria have been frequently isolated from algal blooming ***,knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal blooms(HABs)is *** this study,we investigated the composition of the microbial community coexisting with harmful alga Karenia mikimotoi and its responses to algal control via nutrient stimulation or by adding algicidal strain in *** microorganisms inhabiting the *** culture consisted of 24 identifi ed phyla,including dominant Proteobacteria(relative abundance 76.24%±7.28%)and Bacteroidetes(22.67%±8.32%).Rhodobacteraceae,Phaeodactylibacter,and Maritimibacter predominated during the algal *** the added nutrient and fermentation broth of algicidal strain Pseudoalteromonas QF1 caused a massive death of *** and substantial changes in the coexisting microbial community,in which Rhodobacteraceae and Phaeodactylibacter signifi cantly decreased,while Halomonas and Alteromonas *** operational taxonomic units(OTUs)analysis indicated that 13 OTUs belonging to Rhodobacteraceae,Maritimibacter,Marivita,Nisaea,Phaeodactylibacter,Citreicella,Halomonas,Alteromonas,Marinobacter,Muricauda,and Pseudoalteromonas dominated the changes of the microbial communities observed in the *** culture with or without ***,this study indicated that microbial community inhabiting *** culture includes potential algicidal bacteria,and improves our knowledge about microbial community succession during biocontrol of *** via nutrient stimulation or by adding isolated algicidal strains.

关键词： Karenia mikimotoi microbial community nutrient stimulation algicidal bacteria Pseudoalteromonas

Comparison of Two Methods to Assess the Size Structure of Phytoplankton Community Assemblages, in Liaodong Bay, China

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Journal of ocean University of China 2019年第5期18卷 1207-1215页

作者： SONG Lun WU Jing DU Jing LI Nan WANG Kun WANG Peng State key laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHaerbin 150090China key laboratory of marine biological resources and ecology liaoning ocean and fisheries science research institute Dalian 116023China

Liaodong Bay is one of the largest semi-enclosed bays located in Bohai Sea,in northeast *** is an important feeding and spawning place,even wintering and breeding migration base for many marine *** size structure of phytoplankton communities is a key feature of marine ecosystems and can be used as an indicator for algae disaster,but the methods to evalute is still ***,we compared the size-fractionated chlorophyll-a method with a high-throughout sequencing method in an evaluation of the size structure of phytoplankton assemblages in Liaodong Bay,China,in *** proportion of picophytoplankton was estimated at just 19%according to the results based on size-fractionated chlorophyll-a,which likely represents a severe *** sequencing method confirmed that many kinds of phytoplankton species appeared in more than one size-fractionated filter membrane,with some even dominating where the filter membrane pore size was greater than the individual phytoplankton *** to the sequencing method,the phytoplankton assemblages in Liaodong Bay were dominated by picophytoplankton(average proportion=44%),followed by the nanophytoplankton(average 32%),a composition that is more consistent with other *** sequencing method provides a more robust way to assess the size structure and taxonomic diversity of marine phytoplankton communities.

关键词： size structure chlorophyll-a high-throughout sequencing Liaodong Bay

Spatiotemporal distribution of size-fractioned phytoplankton in the Yalu River Estuary,China

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Ecosystem Health and Sustainability 2022年第1期8卷 264-278页

作者： Yin Liu Lun Song GuangJun Song JinHao Wu Kun Wang ZaoHui Wang SuXuan Liu College of fisheries and Life science Dalian Ocean UniversityDalianChina key laboratory of marine biological resources and ecology Liaoning Ocean and Fisheries Science Research InstituteDalianChina College of marine sciences and Technology and Environment Dalian Ocean UniversityDalianChina

The grain size structure of phytoplankton has great influence on shellfish *** present study aimed to assess the spatial and temporal variation in the phytoplankton community structure in the Yalu River Estuary and to explore the relationship between the phytoplankton community structure and various environmental parameters in ***-throughput sequencing was used in this *** results showed that nanophytoplankton,especially Karlodinium veneficum,dominated the estuary throughout the *** biomass ratio of picophytoplankton,nanophytoplankton,and microphytoplankton were 20:63:17 in spring,30:44:26 in summer,1:38:61 in autumn,and 2:45:53 in winter,***,Dinophyta had the greatest biomass throughout the year,followed by *** the spatial dimension(Station average),COD,T,SST had a positive impact on total phytoplankton communities,and Dep had a negative *** the time dimension(Monthly average),the environmental factor that significantly controlled the phytoplankton community structure were NO2 and SST.

关键词： Eukaryotic phytoplankton high-throughput sequencing particle size and structure environmental factors Yalu River Estuary

Early Embryo and Larval Development of Inviable Intergeneric Hybrids Derived from Crassostrea angulata and Saccostrea cucullata

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Journal of ocean University of China 2016年第3期15卷 515-522页

作者： SU Jiaqi WANG Zhaoping ZHANG Yuehuan YAN Xiwu LI Qiongzhen YU Ruihai key laboratory of Mariculture of Ministry of Education Fisheries College Ocean University of China key laboratory of Tropical marine biological resources and ecology South China Sea Institute of OceanologyChinese Academy of Sciences Engineering research Center of Shellfish Culture and Breeding of liaoning Province College of Fisheries and Life Science Dalian Ocean University key laboratory of Mariculture Guangxi Academy of Fishery Science

To detect the intergeneric hybridization between the oyster Crassostrea angulata and Saccostrea cucullata coexisting along the southern coast of China, reciprocal crosses were conducted between the two species. Barriers for sperm recognizing, binding, penetrating the egg, and forming the pronucleus were detected by fluorescence staining. From the results, although fertilization success was observed in hybrid crosses, the overall fertilization rate was lower than that of intraspecific crosses. A large number of hybrid larvae died at 6–8 d after hatching, and those survived could not complete metamorphosis. C. angulata ♀× S. cucullata ♂ larvae had a growth rate similar to that of the maternal species, whereas S. cucullata ♀× C. angulata ♂ larvae grew the slowest among all crosses. Molecular genetics analysis revealed that hybrid progeny were amphimixis hybrids. This study demonstrated that hybrid embryos generated by crossing C. angulata and S. cucullata could develop normally to the larval state, but could not complete metamorphosis and then develop to the spat stage. Thus, there is a post-reproductive isolation between C. angulata and S. cucullata.

关键词： Crassostrea angulata Saccostrea cucullata intergeneric hybridization hybrid inviability reproductive isolation

Species Composition and Protection of Fish Eggs and Larvae in Spawning Ground of Liaodong Bay

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Animal Husbandry and Feed science 2018年第3期10卷 151-159,177页

作者： Yu Xuguang Dong Jing Wang Bin Wang Xiaolin Fu Jie Chai Yu Wang Aiyong Li Yiping Li Yulong Liu Xiuze Duan Yan Guo Dong liaoning key laboratory of marine biological resources and ecology Liaoning Ocean and Fisheries Science Research Institute

[Objective] The paper was to explore the species composition, number distribution and seasonal dynamics of fish eggs and larvae in Liaodong Bay in 2008. The protection and exploitation status of spawning ground in Liaodong Bay was analyzed, and the corresponding protection measures were put forward. [Methods] The species composition of fish eggs and larvae in spawning ground of Liaodong Bay was investigated by horizontal trawling associated with vertical trawling with zooplankton net in May, July, September and October in 2008. [Result] It was found that 7 724 eggs and 229 larvae of fishes belonging to 32 taxa were collected, in which 29 taxa were identified as species, belonging to 9 genera, 21 families and 28 orders, 2 taxa were identified to only family level, and one failed to be identified. The main spawning groups were Cynoglossus joyneri, Engraulis japonicus, Johnius belengerii, Larimichthys polyactis, Konosirus punctatus, Sardinella zunasi, and Scomberomorus niphonius, and the main spawning ground was found in northern waters and Changxing Island waters in Liaodao Bay. There was slightly less spawning species in 2008 compared with 1998, especially the number of fish eggs and larvae decreased greatly. [Conclusion] The effective measures for protection of spawning grounds in Liaodong Bay are suggested, including control of fishing intensity, development of artificial reefs, establishment of germplasm resources reserves, and standardization of compensation standards for marine ecological damage.

关键词： Liaodong Bay Fish egg Fish larva Species composition

Distinct taxonomic and ecological functions of microbiome in sediments of different depth in Bohai Sea and Yellow Sea

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Journal of oceanology and Limnology 2023年第5期41卷 1765-1780页

作者： Siqi LI Ye CHEN Lun SONG Tiezhu MI Yu ZHEN Zhigang YU College of marine Life science Ocean University of ChinaQingdao 266003China laboratory for marine ecology and Environmental science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237Chin key laboratory of marine Environment and ecology Ministry of EducationOcean University of ChinaQingdao 266100China key laboratory of Gas Hydrate Ministry of Natural ResourcesQingdao Institute of Marine GeologyQingdao 266071China liaoning ocean and fisheries science research institute Dalian 116023China College of Environmental science and Engineering Ocean University of ChinaQingdao 266100China key laboratory of marine Chemistry Theory and Technology Ministry of Education/Institute for Advanced Ocean StudyOcean University of ChinaQingdao 266100China

Microorganisms in sediments are critical to marine *** microbial communities in marine sediments of the Bohai Sea(BS)and Yellow Sea(YS),the eastern Chinese marginal seas,were uncovered in employing the metagenomic *** addition,the taxonomic and functional compositions of microbes were compared among various sediment core *** showing the compositions,diversities,and functions of the microbial communities showed no significant variations with sea areas,and significant seasonal variations was observed in station 3500-7 only on functions of the microbial ***,the compositions,diversities,and functions of the microbial communities changed noticeably in different sediment depths in close correlation with physical and chemical properties of ***,the large fraction of the variation in functional communities remained *** bioinformatic analysis of the metagenomic data,the carbon-metabolismrelated genes such as glycosyl transferase(GT),glycoside hydrolase(GH),and carbohydrate esterase(CE)genes were rich in the microbial community,especially in the top sediment ***,in this N-polluted habitat,nitrification and anaerobic ammonia oxidation(anammox)were dominant in the top sediment depth,whereas dissimilatory nitrate reduction to ammonium(DNRA)and denitrification were dominant in the middle and bottom depth,*** identification of possible biogeochemical links suggested that Gammaproteobacteria,Alphaproteobacteria,Nitrospirae,Nitrospinae,Chloroflexi,and Methanomicrobia might promote effective circulation of carbon and nitrogen *** study expanded our knowledge about the structure and functional potential of microbial communities associated with different sediment depth,and provided further comprehensive information on element cycles in marine environments.

关键词： microbial community function metagenome sequencing marine sediments

The Genetic Structure and Diversity of Repomucenus curvicornis Inhabiting liaoning Coast Based on Mitochondrial COⅠ Gene and Control Region

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Animal Husbandry and Feed science 2018年第1期10卷 12-17页

作者： Li Yulong Liu Xiuze Yu Xuguang Li Yiping Fu Jie Dong Jing liaoning Provincial key laboratory of marine biological resources and ecology Liaoning Ocean and Fisheries Science Research Institute

[Object] This study was conducted to explore the genetic diversity and structure of the wild Repomucenus curvicornis inhabiting liaoning Coast, China. [Method] The mitochondrial COⅠ gene and control region（CR） were PCR amplified from the wild R. curvicornis populations from the Liaodong Bay（n=22） and the northern Yellow Sea（n=18）, sequenced and analyzed for genetic diversity. [Result] The contents of A, T, C and G of 624 bp COⅠ gene were 24.09%, 31.04%, 25.28%, and 19.59%, and those of 460 bp CR fragment were 32.96%, 32.80%, 14.86% and 19.38%, respectively. The total number of variable sites, average number of nucleotide differences（ k）, haplotype diversity（H） and nucleotide diversity（π） based on COⅠ gene were 38, 4.67,（0.96±0.02） and（0.007 5±0.004 2）, and those based on CR fragment were 26, 3.35,（0.97 ±0.02） and（0.007 3±0.004 3）, respectively. Based on mitochondrial COⅠ gene and CR, the genetic diversity of Liaodong Bay population was lower than that of the northern Yellow Sea population. The AMOVA analysis based on CR fragments revealed almost significant genetic divergence between the Liaodong Bay and the northern Yellow Sea populations, while there was no significant genetic divergence based on COⅠ gene. The results showed that CR and COⅠ gene are effective molecular markers for detecting the genetic diversity of R. curvicornis population, while CR is more reliable than COⅠ gene in detecting the genetic structure. [Conclusion] CR is an appropriate marker for genetic analysis of marine fish population.

关键词： Repomucenus curvicornis Mitochondrial DNA COⅠ gene Control region sequence Genetic diversity Genetic differentiation

Interspecific Hybridization Between Crassostrea angulata and C. ariakensis

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Journal of ocean University of China 2015年第4期14卷 710-716页

作者： YAO Tuo ZHANG Yuehuan YAN Xiwu WANG Zhaoping LI Dongchun SU Jiaqi YU Ruihai fisheries College Ocean University of China key laboratory of Tropical marine Bio-resources and ecology South China Sea Institute of Oceanology Chinese Academy of Sciences Engineering research Center of Shellfish Culture and Breeding of liaoning Province College of Fisheries and Life Science Dalian Ocean University

lnterspecific hybridization can generate heterosis, which is proven to be a useful tool in selective breeding programs for oyster culture. Crassostrea angulata and C. ariakensis are two important economic shellfish species in China. We conducted 2 × 2 reciprocal crosses to determine whether these two species can cross-fertilize and their hybrids can hatch, survive and perform hetero- sis. Fertilization was found symmetrical without delay. The rate of fertilization success of C. angulata ♀× C. ariakensis ♂ was lower than that of C. ariakensis ♀ x C. angulata ♂, and the success rate of both hybridizations was lower than that of two intraspecific crosses each. During the planktonic period, survival rate of the progeny was lower in the hybrid crosses than in the intraspecific crosses. On day 360, mean shell height of the progeny of C. angulata ♀ x C. angulata ♂ was highest, which was followed by that of C. angulata ♀ × C. ariakensis ♂, C. ariakensis ♀x C. ariakensis ♂ and C. ariakensis ♀× C. angulata ♂ in a descending order. Mor- phology of adults produced by the hybrid crosses was similar to that of C. angulata. Both hybrids underwent normal gonad devel- opment and produced mature gametes in the mating season. This study provided new insights into the quantitative traits in interspeci- fic crosses of Crassostrea species, thus being of guidance value for selective breeding of oyster.

关键词： Crassostrea angulata Crassostrea ariakensis interspecific hybridization gonad development heterosis

Toxic Algae and Early Warning Management in Yellow Sea and Bohai Sea of China

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Animal Husbandry and Feed science 2014年第2期6卷 83-86页

作者： Song Lun Song Guangjun Song Yonggang Xu Xiaohong liaoning ocean and fisheries science research institute Liaoning Key Laboratory of Marine Biological Resources and Ecology

The research status of toxic algae in Yellow Sea and Bohai Sea are reviewed from the aspects of toxicity characteristics,toxic mechanism and early warning management,and the existing toxic algae and their toxicity in Yellow Sea and Bohai Sea are analyzed in the paper. The early warning level of toxic algae in Yellow Sea and Bohai Sea of China is put forward,and the research direction of shellfish poisoning in future is summarized.

关键词： Toxic algae Shellfish poisoning Early warning management Yellow Sea and Bohai Sea