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Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates

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Journal of Oceanology and Limnology 2025年第1期43卷 196-209页

作者： Qishan SUO Zhenzhen DENG Yang YUE Jing WANG Ning WU Lihua GENG Quanbin ZHANG CAS and Shandong Province key laboratory of Experimental marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China laboratory for marine Biology and Biotechnology Qingdao Marine Science and Technology CenterQingdao 266237China University of Chinese Academy of Sciences Beijing 101408China

Safety,high protein content,and the numerous health benefits make Spirulina an outstanding source of bioactive *** from Spirulina,which exhibit antioxidant,anti-inflammatory,and antihypertensive effects,may assist in the management of cardiovascular diseases(CVDs).Here,we present the optimization of enzyme-digested hydrolysates derived from Spirulina *** using response surface methodology(RSM).We also evaluated the biological activity of Spirulina protein hydrolysates(SPHs)in contributing to the amelioration of hypertension and associated cardiovascular *** findings suggest that Spirulina protein extracts contain a complex proteinaceous composition with phycocyanin being the dominant protein,as evidenced by both SDS-PAGE and LC-MS/MS *** protease,protease K,andα-chymotrypsin exhibited a substantial hydrolytic effect on Spirulina protein,taking into account their degree of hydrolysis(DH),peptide yield,and molecular weight ***-factor experiments identified pH,temperature,and enzyme/substrate ratio as the key factors influencing ***,optimal hydrolysis conditions for the proteases were determined via RSM,leading to a considerable enhancement of the actual *** showed that SPHs have strong antioxidant activity against various free radicals,with a higher ability to scavenge hydroxyl ***,SPHs inhibited macrophage nitric oxide secretion and significantly inhibited angiotensin I-converting enzyme in vitro at 400μg/*** is worth noting that SPHs prepared with protease K exhibited a potent anti-hypertensive effect in *** findings indicate the potential usefulness of including dietary SPHs in preventing hypertension and associated *** research could be valuable in guiding the development of health-promoting foods within the Spirulina industry.

关键词： antioxidant activity antihypertensive effect angiotensin converting enzyme inhibition response surface methodology Spirulina

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Efficient photodegradation of perfluoroalkyl substances under visible light by hexagonal ZnIn_(2)S_(4) nanosheets

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Journal of Environmental Sciences 2025年第2期148卷 116-125页

作者： Fuyu Liu Hui Xue Tingshuo Kang Qiupei Lei Jiazhe Chen Zhihong Zuo Bin Han Xize Lu Xiao Yang Xiangcheng Shan Xiaoyang Song Qingzhe Zhang Yongguang Yin Yong Cai Shandong key laboratory of Environmental Processes and Health Qingdao Key Laboratory of Marine Pollutant PreventionSchool of Environmental Science and EngineeringShandong UniversityQingdao 266237China Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237China Shenzhen Research Institute of Shandong University A301 Virtual University Park in South DistrictShenzhen 518057China State key laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 10085China Department of Chemistry and Biochemistry Florida International UniversityMiamiFlorida 33199USA

Perfluoroalkyl substances(PFASs)are typical persistent organic pollutants,and their removal is urgently required but *** has shown potential in PFASs degradation due to the redox capabilities of photoinduced charge carriers in ***,hexagonal ZnIn_(2)S_(4)(ZIS)nanosheets were synthesized by a one-pot oil bath method and were well characterized by a series of *** the degradation of sodium p-perfluorous nonenoxybenzenesulfonate(OBS),one kind of representative PFASs,the assynthesized ZIS showed activity superior to P25 TiO_(2) under both simulated sunlight and visible-light *** good photocatalytic performance was attributed to the enhanced light absorption and facilitated charge *** pH conditions were found crucial in the photocatalytic process by influencing the OBS adsorption on the ZIS *** e−and h+were the main active species involved in OBS degradation in the ZIS *** work confirmed the feasibility and could provide mechanistic insights into the degradation and defluorination of PFASs by visible-light photocatalysis.

关键词： PFASs Defluorination Photocatalysis Visible light ZnIn_(2)S_(4)

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Effect of gradual increase of salt on performance and microbial community during granulation process

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Journal of Environmental Sciences 2025年第1期147卷 404-413页

作者： Mengfei Wang Junguo He Xiangke Dong Ruimiao Zhang School of Environment Harbin Institute of TechnologyHarbin 150090China State key laboratory of Urban Water Resources and Environment Harbin Institute of TechnologyHarbin 150090China School of Civil Engineering Guangzhou UniversityGuangzhou 510006China National marine Environmental Monitoring Center Dalian 116023China

Salinity was considered to have effects on the characteristics,performance microbial communities of aerobic granular *** study investigated granulation process with gradual increase of salt under different *** identical sequencing batch reactors were operated,while the influent of Ra and Rb was subjected to stepwise increments of NaCl concentrations(0-4 g/L and 0-10 g/L).The presence of filamentous bacteria may contribute to granules formed under lower salinity conditions,potentially leading to granules *** removal efficiency achieved in both reactors although therewas a small accumulation of nitrite in Rb at later *** removal efficiencies of chemical oxygen demand(COD),total nitrogen(TN),and total phosphorus(TP)in Ra were 95.31%,93.70%and 88.66%,while the corresponding removal efficiencies in Rb were 94.19%,89.79%and 80.74%.Salinity stimulated extracellular polymeric substances(EPS)secretion and enriched EPS producing bacteria to help maintain the integrity and stability of the aerobic *** nitrifying bacteria were responsible for NH_(4)^(+)-N and NO_(2)^(-)-N oxidation of salinity systems and large number of denitrifying bacteria were detected,which ensure the high removal efficiency of TN in the systems.

关键词： Aerobic granular sludge(AGS)Salinity Nutrient removal Extracellular polymeric substances(EPS) Microbial community

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Genome-wide transcriptional response of the Arctic bacteriumPseudoalteromonas sp. A2 to oxidative stress induced byhydrogen peroxide

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Acta Oceanologica Sinica 2016年第12期35卷 73-80页

作者： LIN Xuezheng WANG Zhena LI Yang LI Jiang key laboratory of marine bioactive substances the First Institute of Oceanography State Oceanic Administration Qingdao 266061 China

Oxidative stress is one of the major challenges faced by Arctic marine bacteria due to the high oxygen concentration of seawater, low temperatures and UV radiations. Transcriptome sequencing was performed to obtain the key functional genes involved in the adaptation to oxidative stress induced by hydrogen peroxide in the Arctic bacterium Pseudoalteromonas sp. A2. Exposure to 1 mmol/L H2O2 resulted in large alterations of the transcriptome profile, including significant up-regulation of 109 genes and significant down-regulation of 174 genes. COG functional classification revealed that among the significantly regulated genes with known function categories, more genes belonging to posttranslational modification, protein turnover and chaperones were significantly up-regulated, and more genes affiliated with chaperones and amino acid transport and metabolism were significantly down-regulated. It was notable that the expressions of eighteen genes affiliated with flagella and four genes affiliated with heat shock proteins were significantly up-regulated. Meanwhile, the expression of nine genes belonging to cytochrome and cytochrome oxidase, and five genes belonging to Ton B-dependent receptor,were significantly down-regulated. Among the eighteen genes with antioxidant activity categorized by GO analysis, the expression of one gene was significantly up-regulated; however, the expressions of two genes were significantly down-regulated. Briefly, RNA-Seq indicated that, except for the classical anti-oxidative genes and stress proteins, genes affiliated with flagella and function unknown played important roles in coping with oxidative stress in Pseudoalteromonas sp. A2. This overall survey of transcriptome and oxidative stress-relevant genes can contribute to understand the adaptive mechanism of Arctic bacteria.

关键词： Arctic Pseudoalteromonas oxidative stress RNA-Seq

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Microbial community structure of Arctic seawater as revealed by pyrosequencing

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Acta Oceanologica Sinica 2016年第6期35卷 78-84页

作者： LI Yang WANG Zhen LIN Xuezheng The First Institute of Oceanography State Oceanic Administration key laboratory of marine bioactive substances State Oceanic Administration

This study aimed to determine the microbial community structure of seawater in（ICE-1） and out（FUBIAO） of the pack ice zone in the Arctic *** 10 L seawater was filtrated by 0.2 μm Whatman nuclepore filters and the environmental genomic DNA was *** conducted a detailed census of microbial communities by *** of the microbial community structures indicated that these two samples had high bacterial,archaeal and eukaryotic *** and Bacteroidetes were the two dominant members of the bacterioplankton community in both samples,and their relative abundance were 51.29% and 35.39%,72.95%and 23.21%,*** was the most abundant archaeal phylum,and the relative abundance was nearly up to 100% in FUBIAO and 60% in *** for the eukaryotes,no_rank_Eukaryota,Arthropoda and no_rank_Metazoa were the most abundant groups in Sample FUBIAO,accounting for 85.29% of the total *** relative abundance of the most abundant phylum in Sample ICE-1,no_rank_Eukaryota and no_rank_Metazoa,was up to 90.69% of the total ***,Flavobacteria and Gammaproteobacteria were the top three abundant classes in the two samples at the bacterial class *** were also differences in the top ten abundant bacterial,archaeal and eukaryotic OTUs at the level of 97% similarity between the two samples.

关键词： Arctic seawater pyrosequencing microbial community

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Transcriptome analysis of the Antarctic psychrotrophic bacterium Psychrobacter sp. G in response to temperature stress

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Acta Oceanologica Sinica 2017年第2期36卷 78-87页

作者： WANG Zhen LI Yang LIN Xuezheng The First Institute of Oceanography Key Laboratory of Marine Bioactive Substances State Oceanic Administration Qingdao 266061 China

The key functional genes involved in temperature adaption of the Antarctic psychrotrophic bacterium Psychrobacter sp. G. were identified by transcriptomic sequencing. We analyzed the global transcriptional profile of Psychrobacter sp. G under cold stress（0°C） and heat stress（30°C）, with the optimal growth temperature 20°C as the control. There were large alterations of the transcriptome profile, including significant upregulation of 11 and 12 transcripts as well as significant downregulation of 47 and 42 transcripts in the cold and heat stress groups, respectively, compared to the control. The expression of various genes encoding enzymes and transcriptional regulators, including Pfp I and Tet R family transcriptional regulators under heat stress, as well as the expression of DEAD/DEAH box helicase and the Icl R family of transcriptional regulators under cold stress, were upregulated significantly. The expression of several genes, most affiliated with Ton Bdependent receptor and siderophore receptor, was downregulated significantly under both heat and cold stress. Many of the genes associated with the metabolism of fatty acid and ABC transporters were regulated differentially under different temperature stress. The results of this survey of transcriptome and temperature stress-relevant genes contribute to our understanding of the stress-resistant mechanism in Antarctic bacteria.

关键词： Psychrobacter temperature stress transcriptome Antarctic

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Interaction of Ca^(2+)and Fe^(3+)in co-precipitation process induced by Virgibacillus dokdonensis and its application

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Journal of Environmental Sciences 2025年第1期147卷 131-152页

作者： Huaxiao Yan Yuping Liu Haojuan Zhang Shengping Jin Zuozhen Han Jusun Woo Maurice E.Tucker Long Meng Xiangqun Chi Chao Han Yanyang Zhao Yueming Zhao Hui Zhao College of Chemical and Biological Engineering College of Earth Science and EngineeringShandong Provincial Key Laboratory of Depositional Mineralization and Sedimentary MineralsShandong University of Science and TechnologyQingdao 266590China laboratory for marine Mineral Resources Center for Isotope Geochemistry and GeochronologyQingdao National Laboratory for Marine Science and TechnologyQingdao 266237China School of Earth and Environmental Sciences Seoul National UniversitySeoul 08826Korea School of Earth Sciences University of BristolBristol BS81RJUK Cabot Institute University of BristolCantock’s CloseBristol BS81UJUK Qingdao West Coast New District First High School Qingdao 266555China

Biomineralization has garnered significant attention in the field of wastewater treatment due to its notable cost reduction compared to conventional *** reinjection water from oilfields containing an exceedingly high concentration of calcium and ferric ions will pose amajor hazard in ***,the utilization of biomineralization for precipitating these ions has been scarcely investigated due to limited tolerance among halophiles towards such extreme *** this study,free and immobilized halophiles Virgibacillus dokdonensis were used to precipitate these ions and the effects were compared,at the same time,biomineralizationmechanisms and mineral characteristicswere further *** results showthat bacterial concentration and carbonic anhydrase activitywere higher when additionally adding ferric ion based on calcium ion;the content of protein,polysaccharides,deoxyribonucleic acid and humic substances in the extracellular polymers also increased compared to *** ions were biomineralized into calcite and vaterite with mul-tiple *** to iron doping,the crystallinity and thermal stability of calcium carbonate decreased,the content of O-C=O,N-C=OandC-O-PO_(3) increased,the stable carbon isotope values became much more negative,andβ-sheet in minerals *** calcium concentrations facilitated ferric ion precipitation,while ferric ions hindered calcium *** immobilized bacteria performed better in ferric ion removal,with a precipitation ratio exceeding 90%.Free bacteria performed better in calcium removal,and the precipitation ratio reached a maximum of 56%.This research maybe provides some reference for the co-removal of calcium and ferric ions from the oilfield wastewater.

关键词： Calcium carbonate Calcium ion Ferric ion Biomineralization Co-removal Immobilization

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Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21

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Journal of Oceanology and Limnology 2018年第4期36卷 1304-1314页

作者： XIE Maisheng LI Jiang HE Peiqing LIN Xuezheng key laboratory of marine bioactive substances The First Institute of Oceanography State Oceanic Administration (SOA) Qingdao 266061 China key laboratory of marine Natural Products of Qingdao Qingdao 266061 China

In this study, an endolytic alginate lyase, named Al163, was identified, cloned, and characterized from the Antarctic bacterium Pseudoalteromonas sp. NJ-21. Comparative sequence analysis showed that the predicted amino acid sequence encoded by al163 belongs to the polysaccharide lyase 6(PL-6) family and has a molecular mass of about 80 kDa. Recombinant enzyme was purified by Ni-Sepharose affinity chromatography. Recombinant Al163 exhibited maximum activity(258 U/mg) at pH 7.0 and 40℃, and thermal stability assays showed retention of almost 90% activity after incubation at 30℃ for 30 min. Al163 activity was stimulated by Cd^(2+), Ca^(2+), Fe^(3+), and Mn^(2+), but inhibited by Cu^(2+), Si^(2+), Fe^(2+), and Ni^(2+). Thin-layer chromatographic analysis indicated that Al163 degraded sodium alginate, poly M, and poly G, generating disaccharides and trisaccharides as the final products. Only a few bacterial strains that produce a bifunctional alginate lyase have been reported. Our results indicate that recombinant Al163 exhibits broad substrate specificity and its products exhibit low degrees of polymerization. Both properties imply high potential for use of the enzyme in several industrial fields, including cosmetics and pharmaceuticals, based on the high demand for biologically active oligosaccharides.

关键词： Pseudoalteromonas sp. alginate lyase PL-6 family

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Phenylquinolinones with antitumor activity from the Indian Ocean-derived fungus Aspergillus versicolor Y31-2

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Chinese Journal of Oceanology and Limnology 2016年第5期34卷 1072-1075页

作者：李培海樊亚琴陈颢晁雅熙杜宁陈军辉 key Lab of marine bioactive substances First Institute of OceanographyState Oceanic Administration(SOA)

Two phenylquinolinones,including one new compound(1) and a previously isolated compound(2),were isolated from the ethyl acetate extracts of the fungus Aspergillus versicolor Y31-2,which was obtained from seawater samples collected from the Indian *** structures of these compounds were established by spectroscopic analyses.4-(3-Hydroxyphenyl)-3-methoxyquinolin-2(1H)-one(1) exhibited moderate cytotoxicity against MCF-7(human breast carcinoma cell line) and SMMC-7721(human liver cancer cell line) cells with IC_(50) values of 16.6 and 18.2 μmol/L,*** the best of our knowledge,this study represents the first reported account of the isolation of compounds 1 and 2 as the secondary metabolites of the seawater derived fungus Aspergillus versicolor from the Indian Ocean.

关键词： phenylquinolinones anti-tumor Aspergillus versicolor

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Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5

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Acta Oceanologica Sinica 2019年第6期38卷 65-74页

作者： Yaxi Chao Shuyan Wang Suqi Wu Jiaqiang Wei Hao Chen key laboratory of marine bioactive substances First Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China laboratory for marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology (Qingdao)Qingdao 266237China

Seven bacterial clones with alginate-utilizing activity were isolated from rotten kelp. By activity test, the Vibrio sp. QD-5 with the potential alginate-degrading capability was chosen to carry out the draft genome sequencing, and the result showed that the Vibrio sp. QD-5 containing an alginate lyase gene cluster. One of these genes, aly-IV, was cloned and characterized for the first time. After overexpression, Aly-IV, with a molecular mass of about 62 kDa and a theoretical isoelectric point (pI) of 5.12, was purified to a specific activity of 1 256.78 U/mg and showed highest activity at 35°C in the Tris-HCl buffer at pH of 8.9. Moreover, the enzyme activity was enhanced by the metal ions of Na+, K+ and Mg2+ under certain concentration. Aly-IV degraded favorably polyG blocks in an endo-type, yielding monomer and dimer as the main products. Due to its high substrate specificity, Aly-IV could be used as a potential tool for production of polyG oligosaccharides with low degree of polymerization (DP) and for determining the fine structure of alginate.

关键词： alginate lyase Vibrio sp. alginate poly-α-L-guluronic acid lyase oligosaccharides

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