检索结果-南通市图书馆

Malignancy Spectrum 2024年第2期1卷 91-98页

作者： Qiang Li Xinguo Liu Zhongying Dai Pengbo He Yuanyuan Ma Guosheng Shen Xiaodong Jin Fei Ye Xiaogang Zheng Ting Zhao Hui Zhang Zheng Li Bingwen Zou Yuehu Pu Weiqiang Chen Institute of Modern Physics Chinese Academy of SciencesLanzhouGansuChina key laboratory of heavy ion radiation biology and medicine of chinese academy of sciences LanzhouGansuChina gansu Provincial key laboratory of Basic Research on heavy ion radiation Application in medicine LanzhouGansuChina University of chinese academy of sciences BeijingChina Division of Thoracic Tumor Multimodality Treatment and Department of radiation Oncology Cancer CenterWest China HospitalSichuan UniversityChengduSichuanChina Medical Equipment Innovation Research Center West China HospitalSichuan UniversityChengduSichuanChina

Basic research on heavy ion cancer therapy such as radiobiology, medicalphysics, and therapeutic technique has been conducted at the Institute ofModern Physics (IMP), chinese academy of sciences since 1995. Based on theachievements acquired in the basic research and the requirements for a heavyion accelerator for radiotherapy purposes, a dedicated heavy ion therapy facilitynamed heavy ion Medical Machine (HIMM) was designed at IMP andconstructed in Wuwei, China. The HIMM facility consists of two electroncyclotron resonance ion sources, one cyclotron as the injector and onesynchrotron as the main accelerator, and four different treatment roomsequipped with passive or active beam delivery systems, and accelerates carbonions up to 400 MeV/u. After the performance inspection of HIMM organized bythe National Medical Device Inspection Center, preclinical tests like cell andanimal radiobiological experiments and dosimetric verification using anthropomorphicphantoms for elucidating the biophysical properties of the carbon ionbeams provided by HIMM were carried out. According to the chinese medicaldevice regulations, a clinical trial in which 46 tumor patients were recruited andtwo hospitals participated was conducted in the HIMM facility, aiming atevaluating the treatment safety and short-term efficacy of the medical device.The success of the clinical trial helped the HIMM facility be authorized by thechinese government as a class III medical device. In this paper, all the aspectsmentioned above are introduced and discussed, and implications for futureimprovements are also given.

关键词： heavy ion cancer therapy clinical trial performance inspection dosimetric verification medical device

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MiR-663a Inhibits radiation-Induced Epithelium-to-Mesenchymal Transition by Targeting TGF-β1

引用

Biomedical and Environmental sciences 2022年第5期35卷 437-447页

作者： QU Pei SHAO Zhi Ang WANG Bing HE Jin Peng ZHANG Ya Nan WEI Wen Jun HUA Jun Rui ZHOU Heng LU Dong DING Nan WANG Ju Fang key laboratory of space radiobiology of gansu province&key laboratory of heavy ion radiation biology and medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina University of chinese academy of sciences Beijing 100049China

Objective miR-663 a has been reported to be downregulated by X-ray irradiation and participates in radiation-induced bystander effect via TGF-β1.The goal of this study was to explore the role of mi R-663 a during radiation-induced Epithelium-to-mesenchymal transition(EMT).Methods TGF-β1 or IR was used to induce EMT.After mi R-663 a transfection,cell migration and cell morphological changes were detected and the expression levels of mi R-663 a,TGF-β1,and EMT-related factors were quantified.Results Enhancement of cell migration and promotion of mesenchymal changes induced by either TGF-β1 or radiation were suppressed by mi R-663 a.Furthermore,both X-ray and carbon ion irradiation resulted in the upregulation of TGF-β1 and downregulation of mi R-663 a,while the silencing of TGF-β1 by mi R-663 a reversed the EMT process after radiation.Conclusion Our findings demonstrate an EMT-suppressing effect by mi R-663 a via TGF-β1 in radiationinduced EMT.

关键词： Epithelium-to-mesenchymal transition(EMT) ionizing radiation TGF-β1 microRNA miR-663a

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SET8 Inhibition Potentiates Radiotherapy by Suppressing DNA Damage Repair in Carcinomas

引用

Biomedical and Environmental sciences 2022年第3期35卷 194-205页

作者： PAN Dong DU Ya Rong LI Rong SHEN Ai Hua LIU Xiao Dong LI Chuan Yuan HU Bu Rong Department of Radiological Health and radiation medicine School of Public Health and ManagementWenzhou Medical UniversityWenzhou 325035ZhejiangChina key laboratory of heavy ion radiation biology and medicine of chinese academy of sciences&key laboratory of space radiobiology of gansu province Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina Department of Dermatology Duke University Medical CenterDurhamNC27710USA

Objective SET8 is a member of the SET domain-containing family and the only known lysine methyltransferase(KMT)that monomethylates lysine 20 of histone H4(H4 K20 me1).SET8 has been implicated in many essential cellular processes,including cell cycle regulation,DNA replication,DNA damage response,and carcinogenesis.There is no conclusive evidence,however,regarding the effect of SET8 on radiotherapy.In the current study we determined the efficacy of SET8 inhibition on radiotherapy of tumors and the underlying mechanism.Methods First,we explored the radiotherapy benefit of the SET8 expression signature by analyzing clinical data.Then,we measured a series of biological endpoints,including the xenograft tumor growth in mice and apoptosis,frequency of micronuclei,and foci of 53 BP1 andγ-H2 AX in cells to detect the SET8 effects on radiosensitivity.RNA sequencing and subsequent experiments were exploited to verify the mechanism underlying the SET8 effects on radiotherapy.Results Low expression of SET8 predicted a better benefit to radiotherapy in lung adenocarcinoma(LUAD)and invasive breast carcinoma(BRCA)patients.Furthermore,genetic deletion of SET8 significantly enhanced radiation treatment efficacy in a murine tumor model,and A549 and MCF7 cells;SET8 overexpression decreased the radiosensitivity.SET8 inhibition induced more apoptosis,the frequency of micronuclei,and blocked the kinetics process of DNA damage repair as 53 BP1 andγ-H2 AX foci remained in cells.Moreover,RNF8 was positively correlated with the SET8 impact on DNA damage repair.Conclusion Our results demonstrated that SET8 inhibition enhanced radiosensitivity by suppressing DNA damage repair,thus suggesting that SET8 potentiated radiotherapy of carcinomas.As new inhibitors of SET8 are synthesized and tested in preclinical and clinical settings,combining SET8 inhibitors with radiation warrants consideration for precise radiotherapy.

关键词： SET8 Histone methylation DNA repair Radiotherapy Carcinoma

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibition of Ciliogenesis Enhances the Cellular Sensitivity to Temozolomide and ionizing radiation in Human Glioblastoma Cells

引用

Biomedical and Environmental sciences 2022年第5期35卷 419-436页

作者： WEI Li MA Wei CAI Hui PENG Shao Peng TIAN Huan Bing WANG Ju Fang GAO Lan HE Jin Peng School of Life sciences Lanzhou UniversityLanzhou 730000GansuChina NHC key laboratory of Diagnosis and Therapy of Gastrointestinal Tumor Gansu Provincial HospitalLanzhou 730000GansuChina key laboratory of space radiobiology of gansu province&CAS key laboratory of heavy ion radiation biology and medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina University of chinese academy of sciences Beijing 100049China

Objective To investigate the function of primary cilia in regulating the cellular response to temozolomide(TMZ)and ionizing radiation(IR)in glioblastoma(GBM).Methods GBM cells were treated with TMZ or X-ray/carbon ion.The primary cilia were examined by immunostaining with Arl13 b andγ-tubulin,and the cellular resistance ability was measured by cell viability assay or survival fraction assay.Combining with cilia ablation by IFT88 depletion or chloral hydrate and induction by lithium chloride,the autophagy was measured by acridine orange staining assay.The DNA damage repair ability was estimated by the kinetic curve ofγH2 AX foci,and the DNAdependent protein kinase(DNA-PK)activation was detected by immunostaining assay.Results Primary cilia were frequently preserved in GBM,and the induction of ciliogenesis decreased cell proliferation.TMZ and IR promoted ciliogenesis in dose-and time-dependent manners,and the suppression of ciliogenesis significantly enhanced the cellular sensitivity to TMZ and IR.The inhibition of ciliogenesis elevated the lethal effects of TMZ and IR via the impairment of autophagy and DNA damage repair.The interference of ciliogenesis reduced DNA-PK activation,and the knockdown of DNA-PK led to cilium formation and elongation.Conclusion Primary cilia play a vital role in regulating the cellular sensitivity to TMZ and IR in GBM cells through mediating autophagy and DNA damage repair.

关键词： Primary cilia Glioblastoma Cellular sensitivity Temozolomide ionizing radiation Autophagy DNA damage response DNA-PK

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Aurora A Kinase Plays a key Role in Mitosis Skip during Senescence Induced by ionizing radiation

引用

Biomedical and Environmental sciences 2023年第10期36卷 903-916页

作者： ZHANG Xu Rui ZHANG Tong Shan ZHANG Ya Nan HUA Jun Rui WANG Ju Fang HE Jin Peng key laboratory of space radiobiology of gansu province&CAS key laboratory of heavy ion radiation biology and medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina National-Local Joint Engineering Research Center of Biodiagnostic&Biotherapy The Second Affiliated HospitalXi’an Jiaotong UniversityXi’an 710004ShaanxiChina University of chinese academy of sciences Beijing 100049China

Objective To investigate the fate and underlying mechanisms of G2 phase arrest in cancer cells elicited by ionizing radiation(IR).Methods Human melanoma A375 and 92-1 cells were treated with X-rays radiation or Aurora A inhibitor MLN8237(MLN)and/or p21 depletion by small interfering RNA(si RNA).Cell cycle distribution was determined using flow cytometry and a fluorescent ubiquitin-based cell cycle indicator(FUCCI)system combined with histone H3 phosphorylation at Ser10(p S10 H3)detection.Senescence was assessed using senescence-associated-β-galactosidase(SA-β-Gal),Ki67,andγH2AX staining.Protein expression levels were determined using western blotting.Results Tumor cells suffered severe DNA damage and underwent G2 arrest after IR treatment.The damaged cells did not successfully enter M phase nor were they stably blocked at G2 phase but underwent mitotic skipping and entered G1 phase as tetraploid cells,ultimately leading to senescence in G1.During this process,the p53/p21 pathway is hyperactivated.Accompanying p21 accumulation,Aurora A kinase levels declined sharply.MLN treatment confirmed that Aurora A kinase activity is essential for mitosis skipping and senescence induction.Conclusion Persistent p21 activation during IR-induced G2 phase blockade drives Aurora A kinase degradation,leading to senescence via mitotic skipping.

关键词： ionizing radiation Senescence G2 arrest Tetraploid Mitosis skipping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Simulated Microgravity Conditions and Carbon ion Irradiation Induce Spermatogenic Cell Apoptosis and Sperm DNA Damage

引用

Biomedical and Environmental sciences 2013年第9期26卷 726-734页

作者： LI Hong Yan ZHANG Hong MIAO Guo Ying XIE Yi SUN Chao DI Cui Xia LIU Yang LIU Yuan Yuan ZHANG Xin MA Xiao Fei XU Shuai GAN Lu ZHOU Xin Department of heavy ion radiation medicine Institute of Modern Physics Chinese Academy of Sciences key laboratory of heavy ion radiation biology and medicine of chinese academy of sciences key laboratory of heavy ion radiation medicine of gansu province University of chinese academy of sciences

Objective To investigate the effect of simulated microgravity and carbon ion irradiation （CIR） on spermatogenic cell apoptosis and sperm DNA damage to the testis of male Swiss Webster mice, and assess the risk associated with space environment. Methods Sperm DNA damage indicated by DNA fragmentation index （DFI） and high DNA stainability （HDS） was measured by sperm chromatin structure assay （SCSA）. Apoptosis of spermatogenic cells was detected by annexin V-propidium iodide assay. Bax （the expression levels of p53） and proliferating cell nuclear antigen （PCNAI were measured by immunoblotting; p53 and PCNA were located by immunohistology. Results HDS, DFI, apoptosis index, and the expression levels of p53 and Bax were detected to be significantly higher in the experimental groups （P〈0.05） compared with those in the control group, however, the PCNA expression varied to a certain degree, p53- and PCNA- positive expression were detected in each group, mainly in relation to the spermatogonic cells and spermatocytes. Conclusion The findings of the present study demonstrated that simulated microgravity and CIR can induce spermatogenic cell apoptosis and sperm DNA damage. Sperm DNA damage may be one of the underlying mechanisms behind male fertility decline under space environment. These findings may provide a scientific basis for protectint~ astronauts and space traveler＇s health and safety.

关键词： Simulated microgravity Carbon ion irradiation Spermatogenic cells apoptosis Sperm DNAdamage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

引用

Biomedical and Environmental sciences 2015年第8期28卷 549-557页

作者： DU Ya Rong PAN Dong CHEN Ya Xiong XUE Gang REN Zhen Xin LI Xiao Man ZHANG Shi Chuan HU Bu Rong gansu key laboratory of space radiobiology Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences Institute of Modern Physics Chinese Academy of Sciences University of chinese academy of sciences Department of radiation Oncology Sichuan Cancer Hospital

Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells (ASCs) under three-dimensional culture condition. Methods ASCs were isolated and cultured in low attachment dishes to form three-dimensional (3D) spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture. Results The 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer (LET) carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs’ potential of cellular transformation was lower after irradiation by soft agar assay. Conclusion These findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress.

关键词： Adipose-derived stem cells Three-dimensional cell culture Irradiation response High-and low-LET irradiation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bone Loss Induced by Simulated Microgravity,ionizing radiation and/or Ultradian Rhythms in the Hindlimbs of Rats

引用

Biomedical and Environmental sciences 2018年第2期31卷 126-135页

作者： ZHANG Ya Nan SHI Wen Gui LI He HUA Jun Rui FENG Xiu WEI Wen Jun WANG Ju Fang HE Jin Peng LEI Su Wen key laboratory of space radiobiology of gansu province&CAS key laboratory of heavy ion radiation biology and medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina University of chinese academy of sciences Beijing 100049China Office of Health Standards Chinese Center for Disease Control and PreventionBeijing 102206China

Objective To better understand the pathological causes of bone loss in a space environment, including microgravity, ionizing radiation, and ultradian rhythms.Methods Sprague Dawley （SD） rats were randomly divided into a baseline group, a control group, a hindlimb suspension group, a radiation group, a ultradian rhythms group and a combined-three-factor group. After four weeks of hindlimb suspension followed by X-ray exposure and/or ultradian rhythms, biomechanical properties, bone mineral density, histological analysis, microstructure parameters, and bone turnover markers were detected to evaluate bone loss in hindlimbs of rats.Results Simulated microgravity or combined-three factors treatment led to a significant decrease in the biomechanical properties of bones, reduction in bone mineral density, and deterioration of trabecular parameters. ionizing radiation exposure also showed adverse impact while ultradian rhythms had no significant effect on these outcomes. Decrease in the concentration of the turnover markers bone alkaline phosphatase （bALP）, osteocalcin （OCN）, and tartrate-resistant acid phosphatase-5b （TRAP-Sb） in serum was in line with the changes in trabecular parameters.Conclusion Simulated microgravity is the main contributor of bone loss. radiation also results in deleterious effects but ultradian rhythms has no significant effect. Combined-three factors treatment do not exacerbate bone loss when compared to simulated microgravity treatment alone.

关键词： Bone loss Microgravity radiation Ultradian rhythms

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections

引用

Biomedical and Environmental sciences 2018年第2期31卷 136-145页

作者： CHEN Ya Xiong XIE Guang Cheng PAN Dong DU Ya Rong PANG Li Li SONG Jing Dong DUAN Zhao Jun HU Bu Rong key laboratory of heavy ion radiation biology and medicine of chinese academy of sciences & key laboratory of space radiobiology of gansu province Institute of Modern Physics Chinese Academy of Sciences Department of Pathogenic biology Chengde Medical University Department for Viral Diarrhea National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Objective Newly identified human rhinovirus C （HRV-C） and human bocavirus （HBoV） cannot propagate in vitro in traditional cell culture models; thus obtaining knowledge about these viruses and developing related vaccines are difficult. Therefore, it is necessary to develop a novel platform for the propagation of these types of viruses.Methods A platform for culturing human airway epithelia in a three-dimensional （3D） pattern using Matrigel as scaffold was developed. The features of 3D culture were identified by immunochemical staining and transmission electron microscopy. Nucleic acid levels of HRV-C and HBoV in 3D cells at designated time points were quantitated by real-time polymerase chain reaction {PCR）. Levels of cytokines, whose secretion was induced by the viruses, were measured by ELISA.Results Properties of bronchial-like tissues, such as the expression of biomarkers CK5, ZO-2, and PCK, and the development of cilium-like protuberances indicative of the human respiration tract, were observed in 3D-cultured human airway epithelial （HAE） cultures, but not in monolayer-cultured cells. Nucleic acid levels of HRV-C and HBoV and levels of virus-induced cytokines were also measured using the 3D culture system.Conclusion Our data provide a preliminary indication that the 3D culture model of primary epithelia using a Matrigel scaffold in vitro can be used to propagate HRV-C and HBoV.

关键词： 3D cell culture Human airway epithelium （HAE） Human rhinovirus C Human bocavirus Propagation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET ionizing Irradiation

引用

Biomedical and Environmental sciences 2021年第7期34卷 540-551页

作者： JIA Rong CHEN Ya Xiong DU Ya Rong HU Bu Rong key laboratory of space radiobiology of gansu province&CAS key laboratory of heavy ion radiation biology and medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100039China Department of radiation medicine School of Public Health and ManagementWenzhou Medical UniversityWenzhou 325035ZhejiangChina

Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation.Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery(MSD),a high-throughput and sensitive electrochemiluminescence measurement technique.Dose estimation equations were set up using multiple linear regression analysis.Results The relative levels of IL-6 at 1 h,IL-5 and IL-6 at 24 h,and IL-5,IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently.The minimum measured levels of IL-5,IL-6 and IL-15 were up to 4.0076 pg/mL,16.4538 pg/mL and 0.4150 pg/mL,respectively.In addition,dose estimation models were established and verified.Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5,IL-6 and IL-15.These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators.Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.

关键词： MSD assay Low or high LET irradiation Mouse plasma Cytokines Dose estimation model

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：