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Prevotella and succinate treatments altered gut microbiota,increased laying performance,and suppressed hepatic lipid accumulation in laying hens

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Journal of Animal Science and Biotechnology 2024年第2期15卷 844-861页

作者： Min Liu Zeyue Kang Xikang Cao Hongchao Jiao Xiaojuan Wang Jingpeng Zhao Hai Lin College of Animal Science and Technology Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and PreventionKey Laboratory of Efficient Utilization of Non‑Grain Feed Resources(Co‑Construction by Ministry and Province)Ministry of Agriculture and Rural AffairsShandong Agricultural UniversityTai’an 271018China

Background This work aimed to investigate the potential benefits of administering Prevotella and its primary metabolite succinate on performance,hepatic lipid accumulation and gut microbiota in laying *** One hundred and fifty 58-week-old Hyline Brown laying hens,with laying rate below 80%and plasma triglyceride(TG)exceeding 5 mmol/L,were used in this *** hens were randomly allocated into 5 groups and subjected to one of the following treatments:fed with a basal diet(negative control,NC),oral gavage of 3 mL/hen saline every other day(positive control,PC),gavage of 3 mL/hen Prevotella melaninogenica(10^(7)CFU/mL,PM)or 3 mL/hen Prevotella copri(10^(7)CFU/mL,***)every other day,and basal diet supplemented with 0.25%sodium succinate(Succinate).The results showed that PM and *** treatments significantly improved laying rate compared to the PC(P0.05).PM treatment increased(P<0.05)the mRNA levels of genes PGC-1βand APB-5B at week 4,and ACC and CPT-1 at week *** results indicated enhanced antioxidant activities at week 8,as evidenced by reduced hepatic malondialdehyde(MDA)level and improved antioxidant enzymes activities in PM and Succinate groups(P<0.05).Supplementing with Prevotella or succinate can alter the cecal ***,the abundance of Prevotella in the Succinate group was significantly higher than that in the other 4 groups at the family and genus levels(P<0.05).Conclusions Oral intake of Prevotella and dietary supplementation of succinate can ameliorate lipid metabolism of laying *** beneficial effect of Prevotella is consistent across different *** finding highlights that succinate,the primary metaboli

关键词： Fatty liver Gut microbiota Laying hen Prevotella Succinate

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Structural properties of residual carbon in coal gasification fine slag and their influence on flotation separation and resource utilization:A review

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International Journal of Minerals,Metallurgy and Materials 2024年第2期31卷 217-230页

作者： Rui Han Anning Zhou Ningning Zhang Kaiqiang Guo Mengyan Cheng Heng Chen Cuicui Li College of Chemistry and Chemical Engineering Xi’an University of Science and TechnologyXi’an 710054China key laboratory of Coal resources Exploration and Comprehensive utilization Ministry of Natural Resources of ChinaXi’an 710021China

Coal gasification fine slag(FS)is a typical solid waste generated in coal *** current disposal methods of stockpil-ing and landfilling have caused serious soil and ecological *** recovery and the high-value utilization of residual carbon(RC)in FS are the keys to realizing the win-win situation of the coal chemical industry in terms of economic and environmental *** structural properties,such as pore,surface functional group,and microcrystalline structures,of RC in FS(FS-RC)not only affect the flotation recovery efficiency of FS-RC but also form the basis for the high-value utilization of *** this paper,the characteristics of FS-RC in terms of pore structure,surface functional groups,and microcrystalline structure are sorted out in accordance with gasification type and FS particle *** reasons for the formation of the special structural properties of FS-RC are analyzed,and their influence on the flotation separation and high-value utilization of FS-RC is *** methods based on the pore structural characterist-ics of FS-RC,such as ultrasonic pretreatment-pore-blocking flotation and pore breaking-flocculation flotation,are proposed to be the key development technologies for improving FS-RC recovery in the *** design of low-cost,low-dose collectors containing polar bonds based on the surface and microcrystalline structures of FS-RC is proposed to be an important breakthrough point for strengthening the flotation efficiency of FS-RC in the *** high-value utilization of FS should be based on the physicochemical structural proper-ties of FS-RC and should focus on the environmental impact of hazardous elements and the recyclability of chemical waste liquid to es-tablish an environmentally friendly utilization *** review is of great theoretical importance for the comprehensive understand-ing of the unique structural properties of FS-RC,the breakthrough of the technological bottleneck in the e

关键词： coal gasification fine slag residual carbon pore structure surface functional groups microcrystalline structure flotation sep-aration resource utilization

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Odorant-binding protein 19 in Monochamus alternatus involved in the recognition of a volatile strongly emitted from ovipositing host pines

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Insect Science 2024年第1期31卷 134-146页

作者： Ruinan Yang Dongzhen Li Shancheng Yi Yi Wei Manqun Wang Hubei Insect resources utilization and Sustainable Pest Management key laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina key laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation InstituteChinese Academy of ForestryBeijingChina

Monochamus alternatus is the primary carrier of pine wood nematodes,which pose a serious threat to Pinus *** many *** emerging *** adults feed on heathy host pines,while matured adults transfer to stressed host pines for mating and *** odorant-binding proteins(OBPs)of *** have been proved to aid in the complex process of host *** clarify the corresponding relations between OBPs and pine volatiles,more OBPs need to be *** this research,MaltOBP19 showed a specific expression in the antennae and mouthparts of ***,and it was marked in 4 types of antenna sensilla by *** binding assays demonstrated the high binding affinity of MaltOBP19 with camphene and myrcene in *** Y-tube olfactory experiments,*** adults were attracted by camphene and RNAi of OBP19 via microinjection significantly decreased their attraction *** induced phobotaxis,but RNAi had no significant effect on this ***,we found that ingesting dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of *** results suggest that MaltOBP19 may play a role in the process of host conversion via the recognition of camphene,which has been identified to be strongly released in stressed host *** addition,it is proved that knockdown of OBP can be achieved by oral administration of bacteria-expressed double-stranded RNA in *** adults,providing a new perspective in the control of ***.

关键词： bacteria-expressed dsRNA camphene Japanese pine sawyer beetle MaltOBP19 RNAi stressed host pines

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Thermodynamic theory of flotation for a complex multiphase solid -liquid system and high-entropy flotation

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International Journal of Minerals,Metallurgy and Materials 2024年第6期31卷 1177-1197页

作者： Shuming Wen State key laboratory of Complex Nonferrous Metal resources Clean utilization Faculty of Land Resource EngineeringKunming University of Science and TechnologyKunming 650093China

The flotation of complex solid–liquid multiphase systems involve interactions among multiple components,the core problem facing flotation ***,the combined use of multicomponent flotation reagents to improve mineral flotation has become an important issue in studies on the efficient use of refractory mineral ***,studying the flotation of complex solid–liquid systems is extremely difficult,and no systematic theory has been developed to *** addition,the physical mechanism associated with combining reagents to improve the flotation effect has not been unified,which limits the development of flotation theory and the progress of flotation *** this study,we applied theoretical thermodynamics to a solid–liquid flotation system and used changes in the entropy and Gibbs free energy of the reagents adsorbed on the mineral surface to establish thermodynamic equilibrium equations that de-scribe interactions among various material components while also introducing adsorption equilibrium constants for the flotation reagents adsorbed on the mineral *** homogenization effect on the mineral surface in pulp solution was determined using the chemical potentials of the material components of the various mineral surfaces required to maintain *** flotation effect can be improved through synergy among multicomponent flotation reagents;its physical essence is the thermodynamic law that as the number of compon-ents of flotation reagents on the mineral surface increases,the surface adsorption entropy change increases,and the Gibbs free energy change of adsorption *** to the results obtained using flotation thermodynamics theory,we established high-entropy flotation theory and a technical method in which increasing the types of flotation reagents adsorbed on the mineral surface,increasing the adsorption entropy change of the flotation reagents,decreasing the Gibbs free energy change,and improving the adsorption efficiency

关键词： multicomponent flotation reagent adsorption entropy change equations of thermodynamic equilibrium adsorption equilibri-um constant high-entropy flotation

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Green manuring relocates microbiomes in driving the soil functionality of nitrogen cycling to obtain preferable grain yields in thirty years

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Science China(Life Sciences) 2024年第3期67卷 596-610页

作者： Guopeng Zhou Kunkun Fan Songjuan Gao Danna Chang Guilong Li Ting Liang Hai Liang Shun Li Jiudong Zhang Zongxian Che Weidong Cao State key laboratory of Efficient utilization of Arid and Semi-arid Arable Land in Northern China/Institute of agricultural resources and Regional Planning Chinese Academy of Agricultural SciencesBeijing 100081China State key laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China College of resources and Environmental Sciences Nanjing Agricultural UniversityNanjing 210095China Institute of Soil&Fertilizer and Resource&Environment Jiangxi Academy of Agricultural SciencesNanchang 330200China Institute of Soil and Fertilizer and water-saving Agriculture Gansu Academy of Agricultural SciencesLanzhou 730070China

Fertilizers are widely used to produce more food, inevitably altering the diversity and composition of soil organisms. The role of soil biodiversity in controlling multiple ecosystem services remains unclear, especially after decades of fertilization. Here, we assess the contribution of the soil functionalities of carbon(C), nitrogen(N), and phosphorus(P) cycling to crop production and explore how soil organisms control these functionalities in a 33-year field fertilization experiment. The long-term application of green manure or cow manure produced wheat yields equivalent to those obtained with chemical N, with the former providing higher soil functions and allowing the functionality of N cycling(especially soil N mineralization and biological N fixation) to control wheat production. The keystone phylotypes within the global network rather than the overall microbial community dominated the soil multifunctionality and functionality of C,N, and P cycling across the soil profile(0–100 cm). We further confirmed that these keystone phylotypes consisted of many metabolic pathways of nutrient cycling and essential microbes involved in organic C mineralization, N_(2)O release, and biological N fixation. The chemical N, green manure, and cow manure resulted in the highest abundances of amoB, nifH, and GH48 genes and Nitrosomonadaceae,Azospirillaceae, and Sphingomonadaceae within the keystone phylotypes, and these microbes were significantly and positively correlated with N_(2)O release, N fixation, and organic C mineralization, respectively. Moreover, our results demonstrated that organic fertilization increased the effects of the network size and keystone phylotypes on the subsoil functions by facilitating the migration of soil microorganisms across the soil profiles and green manure with the highest migration rates. This study highlights the importance of the functionality of N cycling in controlling crop production and keystone phylotypes in regulating soil functions, an

关键词： soil biodiversity soil multifunctionality element cycling keystone phylotypes

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Soybean(Glycine max)rhizosphere organic phosphorus recycling relies on acid phosphatase activity and specific phosphorusmineralizing-related bacteria in phosphate deficient acidic soils

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Journal of Integrative Agriculture 2024年第5期23卷 1685-1702页

作者： Qianqian Chen Qian Zhao Baoxing Xie Xing Lu Qi Guo Guoxuan Liu Ming Zhou Jihui Tian Weiguo Lu Kang Chen Jiang Tian Cuiyue Liang Root Biology Center State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642China College of Natural resources and Environment South China Agricultural UniversityGuangzhou 510642China Henan Academy of Crops Molecular Breeding Henan Academy of Agricultural SciencesZhengzhou 450002China

Bacteria play critical roles in regulating soil phosphorus(P) cycling. The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood. Here, six soybean(Glycine max) genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments. The acid phosphatase(AcP) activities, organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils. The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils. Soil P-availability affected the ɑ-diversity of bacteria in both bulk and rhizosphere soils. However, soybean had a stronger effect on the bacterial community composition, as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments. The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments. Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils, which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella, and lower relative abundance of Xanthomonas in rhizosphere soils. Among them, Cupriavidus was the dominant phoC bacterial genus, and it was negatively correlated with the soil organic-P concentration. These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils, which might be partially achieved by recruiting specific phoCharboring bacteria, such as Cupriavidus.

关键词： organic phosphorus acid phosphatase soybean bacterial community phoC-harboring bacteria rhizosphere

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Environmental benefits and farmers' adoption of winter cover crops in the North China Plain

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Pedosphere 2024年第1期34卷 159-169页

作者： Shufang GUO Yitao ZHANG Limei ZHAI Jian LIU Hongyuan WANG Hongbin LIU State key laboratory of Efficient utilization of Arid and Semi-arid Arable Land in Northern China Key Laboratory of Nonpoint Source Pollution ControlMinistry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Institute of agricultural Environment and resources Yunnan Academy of Agricultural SciencesKunming 650205China Shandong Yucheng Agro-ecosystem National Observation Research Station Key Laboratory of Ecosystem Network Observation and ModelingInstitute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China The Norwegian Institute of Bioeconomy Research(NIBIO) Akershus 1431Norway

The introduction of cover crops into monoculture systems to improve soil health has been widely adopted worldwide. However, little is known about the environmental risks and application prospects of different cover crops in spring maize(Zea mays L.) monocultures proposed in the North China Plain. A pot experiment was conducted to evaluate the effects of different winter cover crops on subsequent maize yield, soil fertility, and environmental risks of nitrogen(N) loss, and a questionnaire survey was conducted to examine factors influencing farmers' willingness to adopt cover crops in the North China Plain. Based on the same fertilization regime during the maize growing period, four winter cover crop treatments were set up, including bare fallow, hairy vetch(Vicia villosa Roth.), February orchid(Orychophragmus violaceus), and winter oilseed rape(Brassica campestris L.). The results indicated that winter cover crops significantly increased subsequent maize yield and soil organic carbon, total N, and microbial biomass carbon and N compared with the bare fallow *** incorporation of cover crops led to a negligible increase in nitrous oxide(N_(2)O) emissions and had a very limited effect on ammonia(NH_(3)) *** incorporation of February orchid and winter oilseed rape decreased nitrate leaching compared with the hairy vetch treatment in the maize growing *** N losses via N_(2)O and NH_(3) emissions and N leaching accounted for 71%–84% of the N surplus. However, yield increase and environmental benefits were not the main positive factors for farmers to accept cover crops. Financial incentive was rated by 83.9% of farmers as an “extremely important” factor, followed by other costs, when considering winter cover cropping. These results indicate that the environmental benefits depend on the type of cover crop. Maintaining high levels of soil fertility and maize yield, providing sufficient subsidies, and encouraging large-area cultivation of cover crops

关键词： cover crop N leaching NH_(3)emission N_(2)O emission spring maize willingness to accept

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Flowering-time regulation by the circadian clock:From Arabidopsis to crops

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The Crop Journal 2024年第1期12卷 17-27页

作者： Mingkang Yang Wenjie Lin Yarou Xu Biyu Xie Baiyin Yu Liang Chen Wei Huang State key laboratory for Conservation and utilization of Subtropical Agro-bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Henry Fok School of Biology&Agriculture Shaoguan UniversityShaoguan 512005GuangdongChina Guangdong Provincial key laboratory of Protein Function and Regulation in agricultural Organisms South China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong laboratory for Lingnan Modern Agriculture Guangzhou 510642GuangdongChina Guangdong Provincial key laboratory of utilization and Conservation of Food and Medicinal resources in Northern Region Shaoguan UniversityShaoguan 512005GuangdongChina

Precise timing of flowering in plants is critical for their growth and reproductive *** factor controlling flowering time is the cycle of light and darkness within a day,known as the *** are classified into long-day,short-day,and day-neutral plants based on light requirements for floral *** the molecular mechanisms that govern this differentiation remain incompletely understood,studies have consistently shown that the circadian clock plays a central role in regulating photoperiod response across diverse plant ***,there is a scarcity of reviews describing the regulatory network linking the circadian clock with photoperiodic *** review summarizes that regulatory network,focusing on the distinct roles of clock genes in long-day and short-day *** also discuss the strategies of clock gene mutations contributing to geographic variation in longday and short-day crops.

关键词： Circadian clock Photoperiod Short-day plants Long-day plants

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Advances in depressants for flotation separation of Cu–Fe sulfide minerals at low alkalinity:A critical review

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International Journal of Minerals,Metallurgy and Materials 2024年第1期31卷 1-17页

作者： Qicheng Feng Wenhang Yang Maohan Chang Shuming Wen Dianwen Liu Guang Han State key laboratory of Complex Nonferrous Metal resources Clean utilization Faculty of Land Resource EngineeringKunming University of Science and TechnologyKunming 650093China Yunnan key laboratory of Green Separation and Enrichment of Strategic Mineral resources Kunming University of Science and TechnologyKunming 650093China

The flotation separation of Cu–Fe sulfide minerals at low alkalinity can be achieved using selective *** the flotation system of Cu–Fe sulfide minerals,depressants usually preferentially interact with the pyrite surface to render the mineral surface hydrophilic and hinder the adsorption of the *** review summarizes the advances in depressants for the flotation separation of Cu–Fe sulfide minerals at low *** advances include use of inorganic depressants (oxidants and sulfur–oxygen compounds),natural polysaccharides (starch,dextrin,konjac glucomannan,and galactomannan),modified polymers (carboxymethyl cellulose,polyacrylamide,lignosulfonate,and tricarboxylate sodium starch),organic acids (polyglutamic acid,sodium humate,tannic acid,pyrogallic acid,salicylic acid,and lactic acid),sodium dimethyl dithiocarbamate,and *** potential application of specific inorganic and organic depressants in the flotation separation of Cu–Fe sulfide minerals at low alkalinity is *** advances in the use of organic depressants with respect to the flotation separation of Cu–Fe sulfide minerals are comprehensively ***,the depression performances and mechanisms of different types of organic depressants on mineral surfaces are ***,several perspectives on depressants vis-à-vis flotation separation of Cu–Fe sulfide minerals at low alkalinity are proposed.

关键词： Cu–Fe sulfide minerals flotation separation selective depressants depression mechanism

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Application of controlled-release urea increases maize N uptake,environmental benefits and economic returns via optimizing temporal and spatial distributions of soil mineral N

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Pedosphere 2024年第1期34卷 222-235页

作者： Mingxue SUN Juan LI Lili ZHANG Xiaomeng SU Ning LIU Xiaori HAN Songjiang WU Zhentao SUN Xiangdong YANG Institute of agricultural resources and Regional Planning Chinese Academy of Agricultural Sciences/State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/Key Laboratory of Plant Nutrition and FertilizerMinistry of Agriculture and Rural AffairsBeijing 100081China agricultural Development Service Center of Zhuanghe Zhuanghe 116499China agricultural Technology Extension Center Wafangdian 116300China College of Land and Environment Shenyang Agricultural UniversityShenyang 100866China

The creation of controlled-release urea (CRU) is a potent substitute for conventional fertilizers in order to preserve the availability of nitrogen (N) in soil,prevent environmental pollution,and move toward green *** main objectives of this study were to assess the impacts of CRU’s full application on maize production and to clarify the connection between the nutrient release pattern of CRU and maize nutrient *** order to learn more about the effects of CRU application on maize yields,N uptake,mineral N (N_(min)) dynamics,N balance in soil-crop systems,and economic returns,a series of field experiments were carried out in 2018–2020 in Dalian City,Liaoning Province,*** were 4 different treatments in the experiments:no N fertilizer input (control,CK);application of common urea at 210 kg ha^(-1)(U),the ideal fertilization management level for the study site;application of polyurethane-coated urea at the same N input rate as U (PCU);and application of PCU at a 20% reduction in N input rate (0.8PCU).Our findings showed that using CRU (i.e.,PCU and 0.8 PCU) may considerably increase maize N absorption,maintain maize yields,and increase N use efficiency (NUE) compared to *** grain yield showed considerable positive correlations with total N uptake in leaf in U and 0.8 PCU,but negative correlations with that in PCU,indicating that PCU caused excessive maize absorption while 0.8 PCU could achieve a better yield response to N ***,PCU was able to maintain N fertilizer in the soil profile 0–20 cm away from the fertilization point,and higher N_(min)content was observed in the 0–20 cm soil layer at various growth stages,particularly at the middle and late growing stages,optimizing the temporal and spatial distributions of N_(min).Additionally,compared to that in U,the apparent N loss rate in PCU was reduced by 36.2%,and applying CRU (PCU and 0.8 PCU) increased net profit by 8.5% to 15.2% with less labor and fertilization *** was co

关键词： apparent N loss fertilization management green agriculture maize yield N balance N use efficiency

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