检索结果-南通市图书馆

Arsenic trioxide inhibits the activity of SphK1 by decreasing the level of phosphatidylserine and phosphatidic acid in the human gastric cancer cell line MGC-803

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

BIOCELL 2022年第3期46卷 737-743页

作者： PEI LIU ZHE CHEN XIAOQIONG MA key laboratory of digestive pathophysiology of zhejiang province Institute of Cancer ResearchFirst Affiliated HospitalZhejiang Chinese Medical UniversityHangzhou310006China

Sphingosine kinase 1(SphK1)is an important synthetase during the synthesis of sphingosine-1-phosphate(S1P)from sphingosine(Sph).Previous studies demonstrated that arsenic trioxide(As_(2)O_(3))could reduce the level of S1P in human gastric cancer cell line MGC-803,indicating that As_(2)O_(3) may inhibit the activity of *** this study,the effect of As_(2)O_(3) on the SphK1 activation pathway was *** blot and quantitative real-time PCR analysis were used to evaluate the changes in protein and mRNA *** multi-dimensional mass spectrometry-based shotgun lipidomics method(MDMS-SL)was used for the quantitative detection of phosphatidylserine(PS)and phosphatidic acid(PA).The results revealed that As_(2)O_(3) did not affect the protein and mRNA expression of SphK1 in the MGC-803 ***,As_(2)O_(3) increased the levels of p-ERK1/2 and CIB1 in the SphK1 activation pathway and decreased the levels of PS and PA in the MGC-803 *** outcomes suggested that As_(2)O_(3) may enhance the activity of SphK1 by increasing the levels of p-ERK1/2 and CIB1 and decrease the activity of SphK1 by decreasing the levels of PS and *** was suggested that the inhibition effect is stronger and resulting in an overall decrease in the activity of SphK1.

关键词： As_(2)O_(3) SphK1 PS PA

Magnolol protects against acute gastrointestinal injury in sepsis by down-regulating regulated on activation,normal T-cell expressed and secreted

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2021年第34期9卷 10451-10463页

作者： Shi-Hao Mao Dan-Dan Feng Xi Wang Yi-Hui Zhi Shu Lei Xi Xing Rong-Lin Jiang Jian-Nong Wu Department of Intensive Care Unit The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina key laboratory of digestive pathophysiology of zhejiang province The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310053Zhejiang ProvinceChina

BACKGROUND Sepsis is a major medical *** is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects,but the mechanism by which it reduces intestinal inflammation in sepsis is yet *** To assess the protective effect of magnolol on intestinal mucosal epithelial cells in sepsis and elucidate the underlying *** Enzyme-linked immunosorbent assay was used to measure tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,and regulated on activation,normal T-cell expressed and secreted(RANTES)levels in serum and ileal tissue in animal *** histopathological changes of the ileal mucosa in different groups were observed under a *** Counting Kit-8 and cell permeability assays were used to determine the concentration of drug-containing serum that did not affect the activity of Caco2 cells but inhibited lipopolysaccharide(LPS)-induced decrease in *** and Western blot assays were used to detect the levels of RANTES,inhibitor of nuclear factor kappa-B kinaseβ(IKKβ),phosphorylated IKKβ(p-IKKβ),inhibitor of nuclear factor kappa-B kinaseα(IκBα),p65,and p-p65 proteins in different groups in *** In rats treated with LPS by intravenous tail injection in the presence or absence of magnolol,magnolol inhibited the expression of proinflammatory cytokines,IL-1β,IL-6,and TNF-αin a dose-dependent *** addition,magnolol suppressed the production of RANTES in LPS-stimulated sepsis ***,in vitro studies suggested that magnolol inhibited the increase of p65 nucleation,thereby markedly downregulating the production of the phosphorylated form of IKKβin LPS-treated Caco2 ***,magnolol inhibited the translocation of the transcription factor nuclear factor-kappa B(NF-κB)from the cytosol into the nucleus and down-regulated the expression level of the chemokine RANTES in LPS-stimulated Caco2 *** Magno

关键词： Sepsis Magnolol Regulated on activation,normal T-cell expressed and secreted Anti-inflammation Lipopolysaccharide Nuclear factor-kappa B

Gas chromatography/mass spectrometry based metabolomic study in a murine model of irritable bowel syndrome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第8期24卷 894-904页

作者： Lei-Min Yu Ke-Jia Zhao Shuang-Shuang Wang Xi Wang Bin Lu Department of Gastroenterology First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina First Clinical Medical College of zhejiang Chinese Medical University Hangzhou 310053Zhejiang ProvinceChina key laboratory of digestive pathophysiology of zhejiang province First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310053Zhejiang ProvinceChina

AIM To study the role of microbial metabolites in the modulation of biochemical and physiological processes in irritable bowel syndrome(IBS).METHODS In the current study, using a metabolomic approach, we analyzed the key metabolites differentially excreted in the feces of control mice and mice with IBS, with or without Clostridium butyricum(C. butyricum) treatment. C57 BL/6 mice were divided into control, IBS, and IBS + C. butyricum groups. In the IBS and IBS + C. butyricum groups, the mice were subjected to water avoidance stress(WAS) for 1 h/d for ten days. Gas chromatography/mass spectrometry(GC-MS) together with multivariate analysis was employed to compare the fecal samples between groups. RESULTS WAS exposure established an appropriate model of IBS in mice, with symptoms of visceral hyperalgesia and diarrhea. The differences in the metabolite profiles between the control group and IBS group significantly changed with the progression of IBS(days 0, 5, 10, and 17). A total of 14 differentially excreted metabolites were identified between the control and IBS groups, and phenylethylamine was a major metabolite induced by stress. In addition, phenylalanine metabolism was found to be the most relevant metabolic pathway. Between the IBS group and IBS + C. butyricum group, 10 differentially excreted metabolites were identified. Among these, pantothenate and coenzyme A(Co A) biosynthesis metabolites, as well as steroid hormone biosynthesis metabolites were identified as significantly relevant metabolic *** The metabolic profile of IBS mice is significantly altered compared to control mice. Supplementation with C. butyricum to IBS mice may provide a considerable benefit by modulating host metabolism.

关键词： Irritable bowel syndrome Metabolite Gas chromatography/mass spectrometry Clostridium butyricum

Predictive value of blood concentration of biologics on endoscopic inactivity in inflammatory bowel disease:A systematic review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2021年第9期27卷 886-907页

作者： Wan-Ting Cao Rong Huang Ke-Fang Jiang Xue-Hui Qiao Jing-Jing Wang Yi-Hong Fan Yi Xu Department of Gastroenterology The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina zhejiang Provincial key laboratory of Gastrointestinal Diseases pathophysiology Hangzhou 310006Zhejiang ProvinceChina

BACKGROUND Although blood concentration of biologics is an important composition of disease management in inflammatory bowel disease(IBD)patients,complexity and uncertainty of biological management encourage many disputes in predicting the outcome of IBD patients through blood concentration of *** To verify the predictive value of blood concentration of biologics on endoscopic inactivity in IBD patients under different *** We searched PubMed/MEDLINE,Embase,and Web of Science up to May 2020 and identified IBD patients as the research cohort as well as the correlations between blood concentration of biologics and endoscopic inactivity in IBD patients as the research *** A total of 23 articles with 30 clinical studies and 1939 IBD patients were *** predictive cut-off value of blood concentration of infliximab on mucosal healing should be 2.7-10.6μg/mL in *** concentration of infliximab reaching 5.0-12.7μg/mL or more increased the probability of fistula healing/closure in perianal fistulizing Crohn's *** concentration of adalimumab reaching 7.2-16.2μg/mL or more could predict mucosal healing in *** predictive cut-off value of blood concentration of adalimumab on fistula healing/closure should be 5.9-9.8μg/mL in perianal fistulizing Crohn's *** concentration of vedolizumab surpassing 25.0μg/mL indicated mucosal healing in ulcerative colitis patients under maintenance therapy and the predictive cut-off value of blood concentration on mucosal healing or endoscopic remission under induction therapy in IBD could be 8.0-28.9μg/*** Blood concentration of biologics should not be utilized to predict endoscopic inactivity of IBD independently due to discrepancies in clinical studies,whereas conducting therapeutic drug monitoring intensively contributes to precise therapy.

关键词： Inflammatory bowel disease Biological blood concentration Endoscopic inactivity Infliximab Adalimumab Vedolizumab

Role of ferroptosis in colorectal cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2023年第2期15卷 225-239页

作者： Ya-Qi Song Xiao-Dan Yan Yi Wang Zhen-Zhen Wang Xin-Li Mao Li-Ping Ye Shao-Wei Li Taizhou Hospital of zhejiang province Zhejiang UniversityLinhai 317000Zhejiang ProvinceChina Department of Gastroenterology Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Institute of digestive Disease Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina key laboratory of Minimally Invasive Techniques&Rapid Rehabilitation of digestive System Tumor of zhejiang province Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina

Colorectal cancer(CRC)is the second deadliest cancer and the third-most common malignancy in the ***,chemotherapy,and targeted therapy have been widely used to treat CRC,but some patients still develop resistance to these *** is a novel non-apoptotic form of cell *** is an iron-dependent non-apoptotic cell death characterized by the accumulation of lipid reactive oxygen species and has been suggested to play a role in reversing resistance to anticancer *** review summarizes recent advances in the prognostic role of ferroptosis in CRC and the mechanism of action in CRC.

关键词： Ferroptosis Colorectal cancer System Xc− Glutathione Glutathione peroxidase 4

Effectiveness and safety of over-the-scope clip in closing perforations after duodenal surgery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2021年第35期27卷 5958-5966页

作者： Zhen-Zhen Wang Xian-Bin Zhou Yi Wang Xin-Li Mao Li-Ping Ye Ling-Ling Yan Ya-Hong Chen Ya-Qi Song Yue Cai Shi-Wen Xu Shao-Wei Li key laboratory of Minimally Invasive Techniques and Rapid Rehabilitation of digestive System Tumor of zhejiang province Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Department of Gastroenterology Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Health Management Center Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Taizhou Hospital of zhejiang province Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina

BACKGROUND Endoscopic resection of duodenal subepithelial lesions(SELs)is a difficult procedure with a high risk of *** present,dealing with perforation after endoscopic resection of duodenal SELs is still considered a great *** To evaluate the effectiveness and safety of an over-the-scope clip(OTSC)in the treatment of perforation post-endoscopic resection of duodenal *** From May 2015 to November 2019,18 patients with perforation following endoscopic resection of duodenal SELs were treated with *** comprising the rate of complete resection,closure of intraprocedural perforation,delayed bleeding,delayed perforation,and postoperative infection were *** The rate of complete removal of duodenal SELs and successful closure of the perforation was 100%.The median perforation size was 1 cm in *** patients had minor intraoperative bleeding,while the remaining 1 patient had considerable amount of bleeding during the *** patients had postoperative abdominal infections,of which 1 patient developed an abscess in the right iliac fossa and another patient developed septic *** 18 patients recovered and were *** delayed bleeding or perforation was *** mean time taken to resume normal diet after the procedure was 6.5 *** mean postoperative hospital stay was 9.5 *** residual or recurrent lesions were detected during the follow-up period(15-66 mo).CONCLUSION Closing a perforation after endoscopic resection of duodenal SELs with OTSCs seems to be an effective and reasonably safe therapeutic method.

关键词： Over-the-scope clip Duodenal subepithelial lesion Endoscopic resection Perforation Effectiveness Safety

Intestinal Microecology:An Important Target for Chinese Medicine Treatment of Non-alcoholic Fatty Liver Disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2020年第10期26卷 723-728页

作者： LI Hong-shan HU Yi-yang Institute of Liver Disease Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghai(201203)China Liver Disease Department Hwa Mei HospitalUniversity of Chinese Academy of SciencesNingboZhejiang Province(315010)China key laboratory for the Diagnosis and Treatment of digestive System Tumors of zhejiang province NingboZhejiang Province(315010)China

Non-alcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases worldwide,causing serious economic and medical ***,Chinese medicine(CM)has become an important means in treating NAFLD in *** microecology(IM)is an important part of the internal environment in the human body and is involved in the occurrence and development of *** this paper,the authors systematically discuss the significance of IM in the pathogenesis of NAFLD and the current status of research on the CM treatment of NAFLD via IM *** combination with our own research practice,we propose that IM is an important target for the treatment of NAFLD with CM and formulate plans for future research to target limitations existing in current studies.

关键词： intestinal microecology non-alcoholic fatty liver disease Chinese medicine

Is appendoscope a new option for the treatment of acute appendicitis?

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第28期30卷 3386-3392页

作者： Shu-Jiong Feng Yi-Feng Zhou Jian-Feng Yang Hong-Zhang Shen Guang-Xing Cui Xiao-Feng Zhang Department of Gastroenterology Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhou 310000Zhejiang ProvinceChina Department of Gastroenterology Key Laboratory of Integrated Traditional Chinese and Western Medicine for Biliary and Pancreatic Diseases of Zhejiang ProvinceHangzhou 310000Zhejiang ProvinceChina Department of Gastroenterology Hangzhou Institute of Digestive DiseasesHangzhou 310000Zhejiang ProvinceChina

Acute appendicitis is a common surgical *** is commonly caused by obstruction of the appendiceal lumen due to fecaliths,tumors,or lymphoid *** over a century,appendectomy has been the primary treatment for acute *** Groves performed the first open appendectomy in *** 1983,Kurt Semm completed the first laparoscopic appendectomy,heralding a new era in ***,appendectomy is associated with certain complications and a rate of negative *** have suggested controversy over the impact of appendectomy on the development of inflammatory bowel disease and Parkinson’s disease,but an increasing number of studies indicate a possible positive correlation between appendectomy and colorectal cancer,gallstones,and cardiovascular *** the recognition that the appendix is not a vestigial organ and the advancement of endoscopic technology,Liu proposed the endoscopic retrograde appendicitis *** is an effective minimally invasive alternative for treating uncomplicated acute *** team has developed an appendoscope with a disposable digital imaging system operated through the biopsy channel of a colonoscope and successfully applied it in the treatment of *** article provides an overview of the progress in endoscopic treatment for acute appendicitis and offers a new perspective on the future direction of appendiceal disease treatment.

关键词： Acute appendicitis Endoscopic technology Endoscopic retrograde appendicitis therapy Appendoscope Appendiceal disease treatment

Bidirectional effects of the tryptophan metabolite indole-3-acetaldehyde on colorectal cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2024年第6期16卷 2697-2715页

作者： Ze Dai Kai-Li Deng Xiao-Mei Wang Dong-Xue Yang Chun-Lan Tang Yu-Ping Zhou Department of Gastroenterology The First Affiliated Hospital of Ningbo UniversityNingbo 315020Zhejiang ProvinceChina Health Science Center Ningbo UniversityNingbo 315211Zhejiang ProvinceChina School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Institute of digestive Disease of Ningbo University Ningbo UniversityNingbo 315020Zhejiang ProvinceChina Ningbo key laboratory of Translational Medicine Research on Gastroenterology and Hepatology Ningbo Key LaboratoryNingbo 315020Zhejiang ProvinceChina

BACKGROUND Colorectal cancer(CRC)has a high incidence and *** studies have shown that indole derivatives involved in gut microbiota metabolism can impact the tumorigenesis,progression,and metastasis of *** To investigate the effect of indole-3-acetaldehyde(IAAD)on *** The effect of IAAD was evaluated in a syngeneic mouse model of CRC and CRC cell lines(HCT116 and DLD-1).Cell proliferation was assessed by Ki-67 fluorescence staining and cytotoxicity *** apoptosis was analysed by flow cytometry after staining with Annexin V-fluorescein isothiocyanate and propidium *** was investigated using the transwell *** blotting and real-time fluorescence quantitative polymerase chain reaction were performed to evaluate the expression of epithelial-mesenchymal transition related genes and aryl hydrocarbon receptor(AhR)downstream *** PharmMapper,SEA,and SWISS databases were used to screen for potential target proteins of IAAD,and the core proteins were identified through the String *** IAAD reduced tumorigenesis in a syngeneic mouse *** CRC cell lines HCT116 and DLD1,IAAD exhibited cytotoxicity starting at 24 h of treatment,while it reduced Ki67 expression in the *** results of flow cytometry showed that IAAD induced apoptosis in HCT116 cells but had no effect on DLD1 cells,which may be related to the activation of *** can also increase the invasiveness and epithelial-mesenchymal transition of HCT116 and DLD1 *** low concentrations(<12.5μmol/L),IAAD only exhibited cytotoxic effects without promoting cell *** addition,predictions based on online databases,protein-protein interaction analysis,and molecular docking showed that IAAD can bind to matrix metalloproteinase-9(MMP9),angiotensin converting enzyme(ACE),poly(ADP-ribose)polymerase-1(PARP1),matrix metalloproteinase-2(MMP2),and myeloperoxidase(MPO).CONCLUSION Indole-3-aldehyde can induce cell apoptosis and inhibit cell prol

关键词： Indole-3-acetaldehyde Colorectal cancer Tryptophan metabolism Apoptosis Epithelial-mesenchymal transition