检索结果-南通市图书馆

Food Quality and Safety 2023年第1期7卷 87-99页

作者：刘雪松李韫同朱俊臻李鹏霞 Institute of Agricultural Facilities and Equipment Jiangsu Academy of Agricultural SciencesNanjingChina Key Laboratory of Cold Chain Logistics Technology for Agro-Products Ministry of Agriculture and Rural AffairsChina Department of Food Science Shenyang Agricultural UniversityShenyangChina School of Food and Biological Engineering Jiangsu UniversityZhenjiangChina Jiangsu key laboratory for Horticultural Crop Genetic Improvement NanjingChina

Melatonin(MT)is a low molecular weight compound with a wide variety of biological functions in plants.It is known to delay senescence in various fruits and vegetables.This study demonstrates that MT(100μmol/L)delayed the senescence of pak choi stored at 20°C.A total of 1121 differentially expressed genes(DEGs)were identified in an MT-treated group in comparison to untreated pak choi.Analysis of DEGs revealed that MT-induced delay in senescence involved reduction in the expression of chlorophyll metabolism genes and regulation of the expression of hormone-related genes and leaf senescence-related transcription factors.MT decreased the activity of chlorophyll metabolism-related enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase,decreased the concentrations of abscisic acid and jasmonic acid,and increased auxin concentration.MT delayed the impact of senescence-related transcription factors BcNAC41 and BcNAC87 on tobacco leaf senescence.This study provides an insight into the molecular mechanisms of MT-induced delay of postharvest senescence in pak choi and confirms its potential as a preservation technique.

关键词： Pak choi melatonin transcriptome postharvest senescence

Melatonin delays leaf senescence in pak choi(Brassica rapa subsp.chinensis)by regulating biosynthesis of the second messenger cGMP

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2024年第1期10卷 145-155页

作者： Xuesong Liu Ronghui An Guofeng Li Shufen Luo Huali Hu Pengxia Li Institute of Agricultural Facilities and Equipment Jiangsu Academy of Agricultural SciencesNanjingJiangsu 210014China School of Food and Biological Engineering Jiangsu UniversityZhenjiangJiangsu 212013China Jiangsu key laboratory for Horticultural Crop Genetic Improvement NanjingJiangsu 210014China Key Laboratory of Cold Chain Logistics Technology for Agro-Products Ministry of Agriculture and Rural AffairsNanjingJiangsu 210014China Jinan Fruit Research Institute All China Federation of Supply and Marketing CooperativesJinanShandong 250220China

Melatonin(MT)is a low molecular weight compound with multiple biological functions in plants.It is known to delay leaf senescence in various species.However,no data are available on the MT signaling pathway in postharvest vegetables.This study demonstrates that MT increases cGMP concentration and the expression of the cGMP synthesis gene BcGC1 in pak choi.The c GMP inhibitor LY83583 destroys effect of MT delaying the leaf senescence.LY83583 also prevents MT treatment from reducing the expression of chlorophyll metabolism-related genes(BcNYC1,BcNOL,BcPPH1/2,BcSGR1/2,and BcPAO)and senescence genes(BcSAG12 and BcSAG21).It also inhibits MT from reducing the activity of the key chlorophyll catabolism enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase.Thus,the ability of MT to maintain high levels of chlorophyll metabolites is also destroyed.The Arabidopsis c GMP synthetic gene mutant atgc1 was used to confirm that delayed leaf senescence caused by MT is mediated,at least in part,by the second messenger c GMP.

关键词： Pak choi Melatonin cGMP Chlorophyll degradation Senescence

Nondestructive determination of the freshness change in bighead carp heads under variable temperatures by using excitation-emission matrix fl uorescence and back-propagation neural networks

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Future Foods

Journal of Future Foods 2022年第2期2卷 160-166页

作者： Ce Shi Zengtao Ji Xinting Yang Zhixin Jia Ruize Dong Ge Shi Information technology Research Center Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China National Engineering Research Center for Information technology in Agriculture Beijing Academy of Agricultural and Forestry SciencesBeijing 100097China National Engineering laboratory for Agri-product Quality Traceability Beijing Academy of Agricultural and Forestry SciencesBeijing 100097China key laboratory of cold chain logistics technology for agro-product Ministry of Agriculture and Rural AffairsBeijing 100097China

This study established back-propagation neural networks(BPNNs)for evaluating the freshness of bighead carp(Hypophthalmichthys nobilis)heads during chilled storage via fluorescence spectroscopy using an excitation-emission matrix(EEM).The total volatile basic nitrogen(TVB-N)and total aerobic count(TAC)of fish increased obviously during storage at 0,4,8,12,and 16°C,while sensory scores decreased with increasing storage time.The EEM fluorescence intensity was measured,and its change was correlated with the freshness indicators of the samples.Three characteristic components of EEM data were extracted by parallel factor analysis,and two freshness indicators were used to construct the EEM-BPNNs model.The results demonstrated that the relative errors of the EEM-BPNNs model for TVB-N and TAC were less than 14%.This result indicated that the EEM-BPNNs model could determine the freshness of fish in cold chains in a rapid and nondestructive way.

关键词： Excitation-emission matrix Freshness Back-propagation neural networks Parallel factor analysis Chilled storage

Development of a RBFNN prediction model for carrot quality based on meteorological temperatures at vegetable stations

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Health 2024年第2期6卷 49-57页

作者： Yu-Tong Yan Zeng-Tao Ji Ce Shi Research Center of Information technology Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China National Engineering Research Center for Information technology in Agriculture Beijing Academy of Agricultural and Forestry SciencesBeijing 100097China National Engineering laboratory for Agri-product Quality Traceability Beijing Academy of Agricultural and Forestry SciencesBeijing 100097China key laboratory of cold chain logistics technology for agro-product Ministry of Agriculture and Rural AffairsBeijing 100097China

To evaluate and predict the quality of carrots during logistics process in North China under extreme temperature conditions,quality indicator changes of carrots were investigated,and temperature-coupled quality prediction models were developed.Seven temperatures were selected from meteorological temperature data by cluster analysis to simulate the changes in extreme temperatures during the short-term transportation of carrots.No carrots rotted during the 48h storage period.Under both isothermal and nonisothermal conditions,weight loss andΔE increased while the firmness and sensory evaluation(SE)decreased.The RBFNN performed better than the Arrhenius model in predicting weight loss andΔE,with R^(2)>0.97,MSEproducts and therefore reducing economic loss of vegetable industry.

关键词： Carrot Extreme temperatures Temperature coupled Arrhenius Radial basis function neural network

Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor （Hymenoptera： Braconidae）

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2014年第4期21卷 415-428页

作者： Hao-Liang Chen Hong-Yu Zhang James E. Throne Kun Yan Zhu State key laboratory of Agricultural Microbiology Institute of Urban and Horticultural Pests Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and Technology Huazhong Agricultural University Wuhan Institute of Plant Protection and agro-products Safety Anhui Academy of Agricultural Sciences Hefei China USDA Agricultural Research Service Center for Grain & Animal Health Research Manhattan KS USA Department of Entomology Kansas State University Manhattan KS USA

Heat shock proteins （HSPs） are known as chaperones that help with folding of other proteins when cells are under environmental stresses. The upregulation of HSPs is essential for cold survival during insect diapause. The ectoparasitoidHabrobracon hebetor, a potential biological control agent, can enter reproductive diapause when reared at low temperature and short photoperiod. However, the expression of HSPs during diapause of H. hebetor has not been studied. In this study, we sequenced and characterized the full-length complementary DNAs of three Hsp70 genes （HhHsp70I, HhHsp70II and HhHsp70IIl） from 11. hebetor. Their deduced amino acid sequences showed more than 80% identities to their counterparts from other insect species. However, the multiple se- quence alignment among the three deduced amino acid sequences of HhHsp70s showed only 46% identities. A phylogenetic analysis of the three HhHsp70s and all other known Hsp70 sequences from Hymenoptera clustered all the Hsp70s into four groups, and the three HhHsp70s were distributed into three different groups. Real-time quantitative poly- merase chain reaction analysis showed that the expression of the three HhHspTO genes in H. hebetor reared at different conditions was quite different. HhHspTOI showed higher relative expression when H. hebetor were reared at 27.5℃ than at two lower temperatures （17.5℃ and 20℃） regardless of the photoperiod, whereas HhHspTOII showed higher ex- pression when H. hebetor were reared at 20℃ and 10 ： 14 L ： D than when reared at 17.5℃ and either 16 ： 8 L ： D or 10 ： 14 L ： D. In contrast, HhHSP7OIIIwas expressed at similar levels regardless of the rearing conditions. These results may suggest functional differences among the three HhHspTO genes in H. hebetor.

关键词： ectoparasitoid gene expression Habrobracon hebetor heat shock protein70 phylogenetic analysis quantitative RT-PCR