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Distance-basedα-amylase biosensor fabricated with amylopectin-coated mesoporous membrane

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Chinese chemical Letters 2024年第1期35卷 446-450页

作者： Binglu Zhao Mashooq Khan Yulin Liu Wenjun Tai Chongyang Mu Wenli Wu Mei Zhao Yaohong Ma Li Yu Jin-Ming Lin Qiongzheng Hu Qilu University of Technology(shandong Academy of Sciences) Shandong Analysis and Test CenterJi’nan 250014China School of Pharmaceutical Sciences Qilu University of Technology(Shandong Academy of Sciences)Ji’nan 250014China key laboratory of Colloid and Interface Chemistry Shandong UniversityMinistry of EducationJi’nan 250100China key laboratory for Biosensors of shandong Province Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Ji’nan 250353China Beijing key laboratory of Microanalytical Methods and Instrumentation MOE Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyDepartment of ChemistryTsinghua UniversityBeijing 100084China

Paper-based biosensors are widely employed in point-of-care testing(POCT)due to their convenience,portability,low cost,and ease of *** study reports an integrated distance-based paper biosensor fabricated with a mesoporous membrane coated with stimuli-responsive *** detection ofα-amylase(AMY)using amylopectin-coated mesoporous membrane is demonstrated as an *** introducing the AMY solution,it is observed that the aqueous solution flows along the paper strip due to AMY-catalyzed hydrolysis of *** flow distance is proportional to the concentration of AMY with a detection limit as low as 4 mU/*** addition,the detection of AMY is demonstrated in human ***,the inhibitory effect of acarbose on AMY is *** reagent-free and disposable biosensor allows single-step rapid detection of the *** approach is very promising for the development of user-friendly,equipment-free,and cost-effective biosensors with remarkable sensitivity and excellent selectivity for disease diagnosis and hypoglycemic drug screening.

关键词： Paper biosensor α-Amylase Stimuli-responsive polymer Mesoporous membrane Distance Point-of-care testing

Controllable selective anchoring of subnanometric Ru clusters or single-atoms/clusters on tungsten nitride for the hydrogen oxidation

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Science China Chemistry 2024年

作者： Qijiao Sun Chuanqiang Wu Yiqiang Sun Yuan Shen Lei Zhao Xiaodong Yang Li Yang Yunxiang Lin Keke Mao Kun Xu School of Chemistry and chemical Engineering Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education Anhui University Information Materials and Intelligent sensing laboratory of Anhui Province Institutes of Physical Science and Information TechnologyAnhui University School of Chemistry and chemical Engineering University of Jinan School of Energy and Environment Anhui University of Technology

The impregnation method in the preparation of metal cluster catalysts typically inadvertently introduces single atoms （SAs） into the substrate. However, the question of whether the introduction of SAs will further improve the catalytic activity of cluster systems for specific reactions such as the hydrogen oxidation reaction （HOR） remains unraveled. Herein, we demonstrate Ru clusters anchored on WN nanowires （RuC/WN） show a higher alkaline HOR catalytic activity in comparison with Ru SAs and nanoclusters （NCs）-coupled catalyst anchored on WN nanowires system （RuC,S/WN）. Notably, the RuC/WN exhibits superb intrinsic catalytic activity with a mass-normalized exchange current density of 890 mA mg-1PGM, which is among the top level of well developed Ru-based HOR catalysts. Both theoretical simulation and experimental investigation suggest that RuC/WN owns an optimized H*and OH*reaction intermediates for the alkaline HOR, therefore resulting in the excellent intrinsic HOR catalytic performance.

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Kinetic Insights into Boron-Based Materials Catalyzed Oxidative Dehydrogenation of Light Alkanes

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Precision Chemistry 2024年第5期2卷 182-192页

作者： Hao Tian Bingjun Xu Beijing National laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China School of Chemistry and chemical Engineering Shandong UniversityJinanShandong 250100China

High selectivity toward alkenes in oxidative dehydrogenation(ODH)of light alkanes makes boron-based materials promising ***,many key mechanistic aspects are still debated due to the challenge of capturing fleeting reaction *** analysis,including determining reaction orders and activation energy,could be informative for reactions involving radical intermediates but has not been extensively *** Review summarizes the current understanding of the apparent alkane reaction order and the apparent activation energy in the boron-catalyzed *** varying compositions and structures,a majority of boron-based catalysts share many common features,induding alkene selectivity,the evolution and the formation of active site,and the apparent kinetic *** common trends could be attributed to the shared gas-phase radical mediated reaction pathways and the formation of active hydroxylated boron oxide species on boron-containing materials under ODH *** of apparent alkane reaction orders and apparent activation energies are sensitive and reliable experimental measures of the contributions of the gas-phase radical-mediated and surface mediated pathways,suggesting the outline of a general mechanistic framework of the boron-catalyzed ODH.

关键词： oxidative dehydrogenation reaction order kinetic analysis radicals-mediated pathway surface-mediated pathway

Enhanced optical imaging and fluorescent labeling for visualizing drug molecules within living organisms

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Acta Pharmaceutica Sinica B 2024年第6期14卷 2428-2446页

作者： Ting Sun Huanxin Zhao Luyao Hu Xintian Shao Zhiyuan Lu Yuli Wang Peixue Ling Yubo Li Kewu Zeng Qixin Chen School of Pharmaceutical Sciences National Key Laboratory of Advanced Drug Delivery SystemMedical Science and Technology Innovation CenterShandong First Medical University&Shandong Academy of Medical SciencesJinan 250062China Departments of Diagnostic Radiology SurgeryChemical and Biomolecular Engineeringand Biomedical EngineeringYong Loo Lin School of Medicine and College of Design and EngineeringNational University of SingaporeSingapore 119074Singapore Institute of Biochemical and Biotechnological Drugs School of Pharmaceutical SciencesCheeloo College of MedicineShandong UniversityJinan 250012China key laboratory of Biopharmaceuticals Postdoctoral Scientific Research WorkstationShandong Academy of Pharmaceutical ScienceJinan 250098China State key laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China School of Life Sciences Science and Technology Innovation CenterShandong First Medical University&Shandong Academy of Medical SciencesJinan 250062China School of Chinese Materia Medica Tianjin University of Traditional Chinese MedicineTianjin 301617China Tianjin Pharmaceutical DA REN TANG Group Corporation Limited Traditional Chinese Pharmacy Research Institute Tianjin 300457China key laboratory of Systems Bioengineering(Ministry of Education) School of Chemistry Engineering and TechnologyTianjin UniversityTianjin 300072China

The visualization of drugs in living systems has become key techniques in modern *** advancements in optical imaging technologies and molecular design strategies have revolutionized drug *** the subcellular level,super-resolution microscopy has allowed exploration of the molecular landscape within individual cells and the cellular response to *** beyond subcellular imaging,researchers have integrated multiple modes,like optical near-infrared II imaging,to study the complex spatiotemporal interactions between drugs and their *** combining these visualization approaches,researchers gain supplementary information on physiological parameters,metabolic activity,and tissue composition,leading to a comprehensive understanding of drug *** review focuses on cutting-edge technologies in drug visualization,particularly fluorescence imaging,and the main types of fluorescent molecules ***,we discuss current challenges and prospects in targeted drug research,emphasizing the importance of multidisciplinary cooperation in advancing drug *** the integration of advanced imaging technology and molecular design,drug visualization has the potential to redefine our understanding of pharmacology,enabling the analysis of drug micro-dynamics in subcellular environments from new perspectives and deepening pharmacological research to the levels of the cell and organelles.

关键词： Optical imaging Drug visualization Fluorophore labeling Therapeutics

MSC-derived exosomes attenuate hepatic fibrosis in primary sclerosing cholangitis through inhibition of Th17 differentiation

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Asian Journal of Pharmaceutical Sciences 2024年第1期19卷 119-134页

作者： Wenyi Chen Feiyan Lin Xudong Feng Qigu Yao Yingduo Yu Feiqiong Gao Jiahang Zhou Qiaoling Pan Jian Wu Jinfeng Yang Jiong Yu Hongcui Cao Lanjuan Li State key laboratory for the Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Medical Center for Infectious DiseasesThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Jinan Microecological Biomedicine shandong laboratory Jinan 250117China key laboratory of Diagnosis and Treatment of Aging and Physic-chemical Injury Diseases of Zhejiang Province Hangzhou 310003China

Primary sclerosing cholangitis(PSC)is an autoimmune cholangiopathy characterized by chronic inflammation of the biliary epithelium and periductal fibrosis,with no curative treatment available,and liver transplantation is inevitable for end-stage *** placentalmesenchymal stem cell(hpMSC)-derived exosomes have demonstrated the ability to prevent fibrosis,inhibit collagen production and possess immunomodulatory properties in autoimmune liver ***,we prepared hpMSC-derived exosomes(Exo^(MSC))and further investigated the anti-fibrotic effects and detailed mechanism on PSC based on Mdr2^(−/−)mice and multicellular organoids established from PSC *** results showed that Exo^(MSC) ameliorated liver fibrosis in Mdr2^(−/−)mice with significant collagen reduction in the preductal area where Th17 differentiation was inhibited as demonstrated by RNAseq analysis,and the percentage of CD4+IL-17A+T cells was reduced both in Exo^(MSC)-treated Mdr2^(−/−)mice(Mdr2^(−/−)-Exo)in vivo and Exo^(MSC)-treated Th17 differentiation progressed in ***,Exo^(MSC) improved the hypersecretory phenotype and intercellular interactions in the hepatic Th17 microenvironment by regulating PERK/CHOP signaling as supported by multicellular ***,our data demonstrate the antifibrosis effect of Exo^(MSC) in PSC disease by inhibiting Th17 differentiation,and ameliorating the Th17-induced microenvironment,indicating the promising potential therapeutic role of Exo^(MSC) in liver fibrosis of PSC or Th17-related diseases.

关键词： Mesenchymal stem cell Exosomes Primary sclerosing cholangitis Fibrosis Organoids Th17

Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites

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Food Science and Human Wellness 2024年第3期13卷 1336-1347页

作者： Ziyan Wang Qiuyue Shi Ying Feng Jiaojiao Han Chenyang Lu Jun Zhou Zhonghua Wang Xiurong Su State key laboratory for Managing Biotic and chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingbo 315000China School of Marine Science Ningbo UniversityNingbo 315000China key laboratory of Aquaculture Biotechnology(Ningbo University) Ministry of EducationNingbo UniversityNingbo 315000China College of Life Sciences Tonghua Normal UniversityTonghua 134000China shandong Beiyou Biotechnology Co. Ltd.Weifang 261000China

Ulcerative colitis(UC)is a recurrent inflammatory bowel disease that imposes a severe burden on families and *** recent years,exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research *** study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV(Pro-Lys-Lys-Val-Val)of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum ***,the polypeptide composition of *** cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF/TOF-MS).Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α(TNF-α)than the original *** animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC;affect serum metabolites through the arachidonic acid,glycerophospholipid and linoleic acid metabolism pathways;and further alleviate UC *** study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.

关键词： Ulcerative colitis Rhopilema esculentum Kishinouye Cnidoblasts Marine bioactive polypeptides Metagenome Serum metabolome

New Antibacterial Dihydropyrones Induced by Co-Culture of Penicillium crustosum PRB-2 and Penicillium citrinum HDN11-186

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Journal of Ocean University of China 2024年第1期23卷 216-220页

作者： YU Guihong ZHOU Luning WU Guangwei LI Dehai shandong Province key laboratory of Applied Mycology and Qingdao International Center on Microbes Utilizing BiogasSchool of Life SciencesQingdao Agricultural UniversityQingdao 266109China School of Medicine and Pharmacy and Sanya Oceanographic InstitutionOcean University of ChinaQingdao 266003China College of chemical Engineering Nanjing Forestry UniversityNanjing 210037China laboratory for Marine Drugs and Bioproducts Laoshan LaboratoryQingdao 266237China

Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum *** structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass *** absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)***-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured *** 1 showed obvious antibacterial activities against Pro-teus ***-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).

关键词： co-culture Penicillium crustosum Penicillium citrinum dihydropyrones antibacterial activity

Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2024年

作者： Sili Wang Jiancheng Huang Yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong Yuanyuan Shi Bin Hong Xiaoying Bian Wen Liu State key laboratory of chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of Sciences State key laboratory of Microbial Technology Shandong University National Health Commission (NHC) key laboratory of Biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science&Peking Union Medical College

Cobalamin (Cbl)-dependent radical S-adenosyl-L-methionine (SAM) proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual *** of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding *** the biosynthetic pathway of chuangxinmycin (CXM),a unique indole alkaloid antibiotic with potent anti-infective activity,functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process,which,however,remained to be reconstituted *** here report the dissection of this enzymatic process,which requires the incorporation of Cxm8,a Cbl-dependent radical SAM protein,with Cxm9,a DUF5825family protein that shares no homology to any proteins of known ***8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner,achieving CXM and a recently identified,C3-dimethylated *** biochemical characterization,isotope labeling,structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase *** is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

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Supersaturation-driven self-assembly formation of ceftriaxone sodium spherulites:From amorphous form to spherulites

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Particuology 2024年第7期90卷 10-19页

作者： Shankun Liang Yuchao Niu Xueyan Hu Wu Xiao Xuehua Ruan Gaohong He Xiaobin Jiang State key laboratory of Fine chemicals Frontiers Science Center for Smart MaterialsSchool of Chemical EngineeringDalian University of TechnologyDalian116024China School of chemical Engineering at Panjin Dalian University of TechnologyPanjin124221China

Drug particles with spherical morphology possess amazing advantages in terms of particle flowability,mechanical properties,drug solubility,and *** growth mechanism of drug spherulite is of great importance for the preparation and regulation of ***,ceftriaxone sodium spherulites were fabricated by the antisolvent crystallization method using dropwise addition of ceftriaxone sodium solution to *** observation of the whole crystallization process combined with electron microscopy technique revealed the spherical growth process from amorphous form to *** the supersaturation of the crystallization system was adjusted,the ceftriaxone sodium crystals transformed from amorphous form to *** the process of antisolvent crystallization with acetone as antisolvent,when the theoretical supersaturation degree S was higher than 2.62,the crystallization system tended to appear amorphous form;when S was between 2.57 and 2.62,the amorphous form transformed into clustered spherulites;when S was less than 2.57,the surface of spherulites will be covered with flaky crystal,which transformed into urchin-like *** the understanding of the spherical growth mechanism,the ceftriaxone sodium spherulites prepared in this research with modified supersaturation control had a low residue of antisolvent acetone,and the flowability was significantly improved.

关键词： Spherulite Ceftriaxone sodium Antisolvent crystallization Supersaturation Online observation