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Genomics, Proteomics & Bioinformatics 2015年第6期13卷 364-370页

作者： Wanlin Zhang Hailing Wang Xiaomin Guan Qing Niu Wei Li Zhejiang key laboratory of Medical genetics Wenzhou Medical University School of laboratory Medicine and Life Science Wenzhou Medical University

KCNQ1 has been identified as a susceptibility gene of type 2 diabetes mellitus （T2DM） in Asian populations through genome-wide association studies. However, studies on the association between gene polymorphism of KCNQ1 and T2DM complications remain unclear. To further ana- lyze the association between different alleles at the single nncleotide polymorphism （SNP） rs2237892 within KCNQ1 and TD2M and its complications, we conducted a case-control study in a Chinese Han population. The C allele of rs2237892 variant contributed to susceptibility to T2DM （odds ratio [OR], 1.45; 95% confidence interval [CI], 1.20 1.75）. Genotypes CT （OR, 1.97; 95% CI, 1.24-3.15） and CC （OR, 2.49; 95% CI, 1.57-3.95） were associated with an increased risk of T2DM. Multivariate regression analysis was performed with adjustment of age, gender, and body mass index. We found that systolic blood pressure （P = 0.015）, prevalence of hypertension （P = 0.037）, and risk of maerovascnlar disease （OR, 2.10; CI, 1.00-4.45） were significantly higher in subjects with the CC genotype than in the combined population with genotype either CT or TT. Therefore, our data support that KCNQ1 might contribute to the higher incidence of patients with T2DM carrying the risk allele C population. is associated with an increased risk for T2DM and hypertension and macrovascular complications in though it needs further to be confirmed in a larger

关键词： KCNQ1 Type 2 diabetes mellitus High-resolution meltinganalysis Hypertension Macrovascular disease Single nucleotidepolymorphism

Potent in vitro Interference of Fleroxacin in DNA-binding,Unwinding and ATPase Activities of Bloom Helicase

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Biomedical and Environmental Sciences 2013年第4期26卷 231-242页

作者： LUO Heng XU Hou Qiang CHEN Xiang DING Mei YANG Qi Xin LI Kun key laboratory of Animal genetics Breeding and Reproduction in Plateau Mountainous RegionMinistry of EducationCollege of Animal ScienceGuizhou University Guizhou key laboratory of Animal genetics Breeding and ReproductionCollege of Animal ScienceGuizhou University Provincial key laboratory for Agricultural Pest Management of Mountainous Region Guizhou University

Objective To study the effect of fleroxacin （FLRX） on biological properties of Bloom （BLM） helicase catalytic core （BLM 642-2290 helicase） in vitro and the molecular mechanism of interaction between the two molecules. Methods DNA-binding and unwinding activities of BLM 642-1290 helicase were assayed by fluorescence polarization and gel retardation assay under conditions that the helicase was subjected to different concentrations of FLRX. Effect of FLRX on helicase ATPase activity was analyzed by phosphorus-free assay based on a colorimetric estimation of ATP hydrolysis-produced inorganic phosphate. Molecular mechanism of interaction between the two molecules was assayed by ultraviolet and fluorescence spectra. Results The DNA unwinding and ATPase activities of BLM 642-1290 helicase were inhibited whereas the DNA-binding activity was promoted in vitro. A BLM-FLRX complex was formed through one binding site, electrostatic and hydrophobic interaction force. Moreover, the intrinsic fluorescence of the helicase was quenched by FLRX as a result of non-radioactive energy transfer. The biological activity of helicase was affected by FLRX, which may be through an allosteric mechanism and stabilization of enzyme conformation in low helicase activity state, disruption of the coupling of ATP hydrolysis to unwinding, and blocking helicase translocation on DNA strands. Conclusion FLRX may affect the biological activities and conformation of BLM 642-1290 helicase, and DNA helicase may be used as a promising drug target for some diseases.

关键词： Biological activity BLM helicase Fleroxacin Interaction mechanism

A rapid method for the detection of humic acid based on the poly(thymine)-templated copper nanoparticles

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Chinese Chemical Letters 2018年第1期29卷 136-138页

作者： Changbei Ma Mingjian Chen Haisheng Liu Kefeng Wu Hailun He Kemin Wang State key laboratory of Medical genetics & School of Life Sciences Central South University State key laboratory of Chemo/Biosensing and Chemometrics Hunan University

This work described a new method for the detection of humic acid （HA） based on the poly（thymine）（polyT）-templated copper nanoparticles （CuNPs）. Without the presence of HA, the formation of poly T- templated CuNPs could take place, resulting in strong fluorescence emission peaks at 610 nm （upon excitation at 340 nm）. On the other hand, when HA was present, strong interaction between HA and Cu2＋ took place, which then hampered the effective formation of fluorescent CuNPs, leading to the decrease in fluorescence intensity. Furthermore, under the optimal experimental conditions, the method exhibited a high specificity to HA with a detection limit of 0.4 mg/L. This work has demonstrated a low-cost and convenient method that could be accomplished within 10 min. The method could provide a simple, rapid, and sensitive fluorescent olatform for the detection of HA.

关键词： Label-freeHumic acidFluorescenceCopper nanoparticleCost-effective

GS6, A Member of the GRAS Gene Family,Negatively Regulates Grain Size in Rice

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Journal of Integrative Plant Biology 2013年第10期55卷 938-949页

作者： Lianjun Sun Xiaojiao Li Yongcai Fu Zuofeng Zhu Lubin Tan Fengxia Liu Xianyou Sun Xuewen Sun Chuanqing Sun State key laboratory of Plant Physiology and Biochemistry National Center for Evaluation of Agricultural Wild Plants (Rice) MOE Key Laboratory of Crop Heterosis and Utilization key laboratory of Crop Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University State key laboratory of Plant Physiology and Biochemistry National Center for Evaluation of Agricultural Wild Plants(Rice) MOE Key Laboratory of Crop Heterosis and Utilization key laboratory of Crop Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University

Grain size is an important yield-related trait in rice. Intensive artificial selection for grain size during domestication is evidenced by the larger grains of most of today＇s cultivars compared with their wild relatives. However, the molecular genetic control of rice grain size is still not well characterized. Here, we report the identification and cloning of Grain Size 6 （GS6）, which plays an important role in reducing grain size in rice. A premature stop at the ＋348 position in the coding sequence （CDS） of GS6 increased grain width and weight significantly. Alignment of the CDS regions of GS6 in 90 rice materials revealed three GS6 alleles. Most japonica varieties （95%） harbor the Type I haplotype, and 62.9% of indica varieties harbor the Type II haplotype. Association analysis revealed that the Type I haplotype tends to increase the width and weight of grains more than either of the Type II or Type III haplotypes. Further investigation of genetic diversity and the evolutionary mechanisms of GS6 showed that the GS6 gene was strongly selected in japonica cultivars. In addition, a ＂ggc＂ repeat region identified in the region that encodes the GRAS domain of GS6 played an important historic role in the domestication of grain size in rice. Knowledge of the function of GS6 might aid efforts to elucidate the molecular mechanisms that control grain development and evolution in rice plants, and could facilitate the genetic improvement of rice yield.

关键词： Domestication GS6 , grain size haplotype Oryza sativa rice.

Cloning of a NaCl-induced fructose-1,6-diphosphate aldolase cDNA from Dunaliella salina and its expression in tobacco

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Science China(Life Sciences) 2003年第1期46卷 49-57页

作者：张晓宁林长发陈火英王昊曲志才张宏伟姚剑虹沈大棱 Institute of genetics State Key Laboratory of Genetic Engineering Ministry of Education Key Laboratory for Bio-diversity Science and Ecological EngineeringSchool of Life SciencesFudan University Agricultural College of Jiaotong University Institute of genetics State Key Laboratory of Genetic Engineering Ministry of Education Key Laboratory for Bio-diversity Science and Ecological Engineering School of Life SciencesFudan University

Using Rapid Amplification of cDNA ends (RACE) technique, the full-length cDNA en-coding a NaCl-induced fructose-1, 6- diphosphate aldolase (DsALDP) was obtained. It was shown that the DsALDP had a relatively high homology (66%—73%) to chloroplast fructose-1, 6-diphos- phate aldolase (AldP) in many plants according to their amino acid sequences. The phylogenetic analysis further confirmed that AldP in alga is the nearest to DsALDP. As to its expression pattern, DsALDP was de novo synthesized by NaCl induction. Its expression level was significantly changed with inducing time. After the selected DsALDP cDNA subcloned into a binary vector pBI121, the new construct was introduced into tobacco by Agrobacterium tumefaciens. The results of Southern blot and RT-PCR analysis of four transgenic T1 plants indicated that DsALDP was integrated into genome of these transgenic plants and effectively expressed. Aldolase activities have been detected in T1-1, T1-2 and T1-3 plants by bioassay under 100—200 mmol/L NaCl. It was also observed that proline contents in them were differentially increased.

关键词： D. salina, NaCl-induced, differentially expressed, chloroplast fructose-1, 6-diphosphate aldolase, salt tolerance.

Molecular Cloning,Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage(Brassica campestris ***)

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Journal of Integrative Agriculture 2013年第12期12卷 2149-2156页

作者： WANG Li GE Ting-ting PENG Hai-tao WANG Cheng LIU Tong-kun HOU Xi-lin LI Ying Horticultural Department Nanjing Agricultural University State key laboratory of Crop genetics and Germplasm Enhancement key laboratory of Southern Vegetable Crop Genetic Improvement Ministry of Agriculture

The exocyst is a conserved protein complex,and required for vesicles tethering,fusion and polarized ***70A1,the exocyst subunit,is essential for assembly of the exocyst *** better understand potential roles of Exo70A1 in non-heading Chinese cabbage（Brassica campestris ***）,we obtained the full-length cDNA of Exo70A1 gene,which consisted of 1 917 bp and encoded a protein of 638 amino *** showed BcExo70A1 shared 94.9% identity with Brassica oleracea ***（AEI26267.1）,and clustered into a same group with other homologues in *** *** and Brassica *** localization analysis showed BcExo70A1 was localized to punctate structures in cytosol of onion epithelial *** showed that BcExo70A1 was widely presented in stamens,young stems,petals,unpollinated pistils,roots and leaves of self compatible and incompatible *** transcripts of BcExo70A1 in non- heading Chinese cabbage declined during initial 1.5 h after incompatible pollination,while an opposite trend was presented after compatible *** study reveals that BcExo70A1 could play essential roles in plant growth and development,and is related to the rejection of self pollen in non-heading Chinese cabbage.

关键词： Exo70A1 gene expression subcellular localization self-incompatibility non-heading Chinese cabbage

Cloning and characterization of an up-regulated GA 20-oxidase gene in hybrid maize

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Progress in Natural Science:Materials International 2009年第2期19卷 161-166页

作者： Jinkun Du,Yingyin Yao,Zhongfu Ni,Qixin Sun Department of Plant genetics & Breeding and State key laboratory for Agrobiotechnology,key laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing key laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100094,China Department of Plant genetics & Breeding and State key laboratory for Agrobiotechnology Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

Previous studies revealed that GA content and metabolism are positively correlated with a faster shoot growth rate of hybrid,and recently,genes participating in both GA biosynthesis and GA response pathways were also found to be differentially expressed between wheat hybrid and its parental inbreds. In this study,an up-regulated GA 20-oxidase gene in a maize hybrid,designated ZmGA20,was cloned. ZmGA20 contains an open reading frame (ORF) encoding 391 amino acid residues. BLASTX searches in GenBank revealed that the ZmGA20 is homologous to the sequences of GA20ox proteins from different species,and analysis also indicated that ZmGA20 had typical features of GA 20-oxidase proteins,including a "LPWKET" sequence. Semi-quantitative RT-PCR analysis showed that ZmGA20 was expressed in different tissues and/or organs. The expression level of ZmGA20 in the hybrid was higher than that in two parents (in roots,leaves,stems and embryo,and ears). The abundance of ZmGA20 transcript was equal to that of the highly expressed parents,which provided molecular evidence for the observed GA content heterosis in maize hybrids.

关键词： GA 20-oxidase Gene expression Maize Heterosis

Overexpression of the ThTPS gene enhanced salt and osmotic stress tolerance in Tamarix hispida

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Journal of Forestry Research 2022年第1期33卷 299-308页

作者： Peilong Wang Xiaojin Lei Jiaxin Lü Caiqiu Gao State key laboratory of Tree genetics and Breeding(Northeast Forestry University) Harbin 150040People’s Republic of China

Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic ***-6-phosphate synthase(TPS)plays important roles in trehalose metabolism and *** this study,the full-length cDNA of ThTPS was cloned from Tamarix hispida Willd.A phylogenetic tree including ThTPS and 11 AtTPS genes from Arabidopsis indicated that the ThTPS protein had a close evolutionary relationship with ***,the function of At TPS7 has not been *** analyze the abiotic stress tolerance function of ThTPS,the expression of ThTPS in *** under salt and drought stress and JA,ABA and GA3 hormone stimulation was monitored by *** results show that ThTPS expression was clearly induced by all five of these treatments at one or more times,and salt stress caused particularly strong induction of Th TPS in the roots of *** ThTPS gene was transiently overexpressed in *** physiological indexes and staining results showed that ThTPS gene overexpression increased salt and osmotic stress tolerance in ***,the Th TPS gene can respond to abiotic stresses such as salt and drought,and its overexpression can significantly improve salt and osmotic *** findings establish a foundation to better understand the responses of TPS genes to abiotic stress in plants.

关键词： Trehalose-6-phosphate synthase(TPS) Tamarix hispida Salt tolerance Osmotic resistance

Analysis of genes related to xylem cell wall development based on transcriptomics in Populus alba ‘Berolinensis’ tension wood

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Journal of Forestry Research 2024年第6期35卷 328-343页

作者： Lin Feng Youchao He Xu Li Meiqi Zhou Chao Wang State key laboratory of Tree genetics and Breeding Northeast Forestry University150040HarbinPeople’s Republic of China

Populus alba‘Berolinensis’is a fast-growing,high-yielding species with strong biotic and abiotic stress resistance,and widely planted for timber,shelter belts and aesthetic *** this study,molecular development is explored and the important genes regulating xylem forma-tion in ***‘Berolinensis’under artificial bending treat-ments was *** investigation indicated that tension wood(TW)was characterized by eccentric growth of xylem and was enriched in cellulose;the degree of ligni-fication was lower than for normal wood(NW)and oppo-site wood(OW).RNA-Seq-based transcriptome analysis was performed using developing xylem from three wood types(TW,OW and NW).A large number of differentially expressed genes(DEGs)were screened and 4889 *** GO and KEGG enrichment results,genes involved in plant hormone signal transduction,phenylpropanoid biosynthesis,and cell wall and secondary cell wall biogenesis play major roles in xylem development under artificial *** expansin(PalEXP)genes were identified from the RNA-seq data;four were differentially expressed during tension wood *** analysis indicated that PalEXLB1 belongs to the EXPB subfamily and that the other PalEXPs are members of the EXPA subfamily.A transcriptional regulatory network construction showed 10 transcription factors located in the first and second layers upstream of EXP,including WRKY,ERF and ***‒qPCR analy-sis in leaves,stems and roots combined with transcriptome analysis suggests that PalEXPA2,PalEXPA4 and PalEXPA15 play significant regulatory roles in cell wall formation during tension wood *** candidate genes involved in xylem cell wall development during tension wood formation marks an important step toward identifying the molecular regulatory mechanism of xylem development and wood property improvement in ***‘Berolinensis’.

关键词： Populus alba‘Berolinensis’ Tension wood Differentially expressed genes Expansin proteins

Ectopic expression of soybean GmKNT1 in Arabidopsis results in altered leaf morphology and flower identity

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Journal of genetics and Genomics 2008年第7期35卷 441-449页

作者： Jun Liu Da Ha Zongming Xie Chunmei Wang Huiwen Wang Wanke Zhang Jinsong Zhang Shouyi Chen National key laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Plant morphology is specified by leaves and flowers, and the shoot apical meristem （SAM） defines the architecture of plant leaves and flowers. Here, we reported the characterization of a soybean KNOX gene GmKNT1, which was highly homologous to Arabidopsis STM. The GmKNT1 was strongly expressed in roots, flowers and developing seeds. Its expression could be induced by IAA, ABA and JA, but inhibited by GA or cytokinin. Staining of the transgenic plants overexpressing GmKNT1-GUS fusion protein revealed that the GmKNT1 was mainly expressed at lobe region, SAM of young leaves, sepal and carpel, not in seed and mature leaves. Scanning electron microscopy （SEM） disclosed multiple changes in morphology of the epidermal cells and stigma. The transgenic Arabidopsis plants overexpressing the GmKNT1 showed small and lobed leaves, shortened internodes and small clustered inflorescence. The lobed leaves might result from the function of the meristems located at the boundary of the leaf. Compared with wild type plants, transgenic plants had higher expression of the SAM-related genes including the CUP, WUS, CUC1, KNAT2 and KNAT6. These results indicated that the GmKNT1 could affect multiple aspects of plant growth and development by regulation of downstream genes expression.

关键词： soybean ectopic expression GmKNT1 KNOX genes development