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Verify the Function of a Potential Growth-Regulating gene in Marine Bivalve Using a Candidate Model Organism Mulinia lateralis

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Journal of Ocean University of China 2023年第4期22卷 1012-1022页

作者： WANG Chen KONG Lingling LIAN Shanshan YANG Zujing MENG Deting LI Moli ZHANG Xiangchao BAO Zhenmin HU Xiaoli MOE key laboratory of Marine genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China laboratory of Tropical Marine Germplasm resources and Breeding engineering Sanya Oceanographic InstitutionOcean University of ChinaSanya 572000China

A better understanding of genetic bases of growth regulation is essential for bivalve breeding,which is helpful to improve the yield of the commercially important *** previous studies have identified some candidate genes accounting for variation in growth-related traits through genotype-phenotype association analyses,seldom of them have verified the functions of these putative,growth-related genes beyond the genomic level due to the difficulty of culturing commercial bivalves under laboratory ***,dwarf surf clam Mulinia lateralis can serve as a model organism for studying marine bivalves given its short generation time,the feasibility of being grown under experimental conditions and the availability of genetic and biological *** dwarf surf clam as a model bivalve,we characterize E2F3,a gene that has been found to account for variation in growth in scallops by a previous genome-wide association study,and verify its function in growth regulation through RNA interference(RNAi)*** the first time,E2F3 in dwarf surf clam,which is termed as MulE2F3,is *** results reveal that dwarf surf clams with MulE2F3 knocked down exhibit a reduction in both shell size and soft-tissue weight,indicating the functions of MulE2F3 in positively regulating bivalve *** importantly,we demonstrate how dwarf surf clam can be used as a model organism to investigate gene functions in commercial bivalves,shedding light on genetic causes for variation in growth to enhance the efficiency of bivalve farming.

关键词： growth-regulating gene function marine bivalve model organism Mulinia lateralis

Characterization and Pathogenicity of Pseudopestalotiopsis vietnamensis Causing Gray Blight of Wuyi Rock Tea(Camellia sinensis)in China and Specific Mechanisms of Disease Infection

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Phyton-International Journal of Experimental Botany 2023年第1期92卷 131-147页

作者： Guangheng Wu Lu Rui Xiang Lu Libo Han Gan Lv Xianyu Fu Jinxian Liu Nong Zhou Chuanhai Zhang Fujian Provincial key laboratory of Eco-Industrial Green Technology College of Ecology and Resources EngineeringWuyi UniversityWuyishan354300China key laboratory of Ministry of Education for genetics Breeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou350002China Chongqing engineering laboratory of Green Planting and Deep Processing of Famous-Region Drug in the Three Gorges Reservoir Region College of Biology and Food EngineeringChongqing Three Gorges UniversityChongqing404120China State key laboratory of Crop gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu611130China College of Tea and Food Science Wuyi UniversityWuyishan354300China

Gray blight disease(GBD)causes significant losses in tea production in *** genes and biological processes involved in resistance to fungal disease in tea plants have been identified,specific mechanisms of the GBD infection process remain *** this study,morphological and multi-gene(TEF-TUB-ITS)phylogenetic characteristics were used to identify isolate CLBB1 of Pseudopestalotiopsis *** tests confirmed that isolate CLBB1 from tea leaves caused GBD in the susceptible tea cultivar Wuyi Rock(Camellia sinensis *** ***).Spores began to germinate 24 h after infection(hai),and after 48 h,elongated fungal hyphae formed from a single *** analysis revealed that 482,517,and 369 genes were differentially expressed at 24,48,and 72 hai,respectively,in Wuyi Rock tea *** CLBB1 infection elicited phenotype-related responses and activated defense-related pathways,including plant–pathogen interaction,MAPK signaling,and plant hormone signal transduction,suggesting a possible mechanism underlying phenotype-based susceptibility to ***,a new *** strain causing GBD in the tea cultivar‘Shuixian’was discovered in this *** analysis indicated that pathogen invasion activated chitin-related MAPK pathways and that tea plants required a hormone to restrict CLBB1.

关键词： Chitin-related MAPK pathways gray blight disease Pseudopestalotiopsis tea transcriptional analysis

Cytological Effects of Space Environment on Different Genotype of Rice

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Journal of Beijing Institute of Technology 2007年第2期16卷 220-225页

作者：魏力军钱宇杨谦徐建龙王俊敏孙野青 Department of Life Science and engineering Harbin Institute of Technology National key Facility for Crop gene resources & genetic Improvement Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences Institute of Environmental Systems Biology Dalian Maritime University

For exploring the biological effect of space environment on different genotype of rice seeds, the cytological effects of M1 generation after space flight were studied. Twelve different genotypes of rice seeds which belong to different climate ecotype （early, medium and late） of indica and japonica were onboard ＂Shenzhou 4＂ spaceship for 162 h. After recovered the total number of mitosis cells and chromosomal aberration were observed. In all the lines the mitotic index （MIs） of space flight are much higher than control, which indicates the stimulate effect of space environment. The cell rate of chromosomal aberration （CRCA） of space flight is also much higher than control, but varies from line to line. It indicates that biological effect of space environment on rice seed dependents not only on flight duration but also on rice genotype. The radiosensitivities of different lines were also discussed according to CRCAs.

关键词： rice space flight cytological effects

Pseudomonas Cyclic Lipopeptide Medpeptin:Biosynthesis and Modulation of Plant Immunity

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engineering 2023年第9期28卷 153-165页

作者： Yi-Lin Gu Jun-Zhou Li Yan Li Shen Cong Jing Wang Yi-Nan Ma Hai-Lei Wei State key laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China Key Laboratory of Microbial Resources Collection and PreservationMinistry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China

The multifunctional secondary metabolites known as cyclic lipopeptides(CLPs),which are produced by a large variety of bacteria,have become a key category of plant immunity ***-CLPs(PsCLPs)are extremely diverse in structure and biological ***,an understanding of CLP-plant structure–function interactions currently remains ***,we identify medpeptin,a novel CLP from Pseudomonas mediterranea that consists of 22 amino *** is synthesized by a non-ribosomal peptide synthase(NRPS)gene cluster and regulated by a quorum-sensing *** research indicates that medpeptin does not exhibit antimicrobial activity;instead,it induces plant cell death immunity and confers resistance to bacterial *** transcriptome analysis and virus-induced gene silencing(VIGS)reveal a set of immune signaling candidates involved in medpeptin *** of a cell-wall leucine-rich repeat extensin protein(NbLRX3)or a receptor-like protein kinase(NbRLK25)—but not BAK1 or SGT1—compromises medpeptin-triggered cell death and resistance to pathogen infection in Nicotiana *** findings point to a noncanonical mechanism of CLP sensing and suggest perspectives for the development of plant disease resistance.

关键词： Pseudomonas Cyclic lipopeptide Cell death Leucine-rich repeat extension(LRX) Medpeptin Receptor-like kinase(RLK)

Pm21, Encoding a Typical CC-NBS-LRR Protein, Confers Broad-Spectrum Resistance to Wheat Powdery Mildew Disease

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Molecular Plant 2018年第6期11卷 879-882页

作者： Huagang He Shanying Zhu Renhui Zhao Zhengning Jiang Yaoyong Ji Jian Ji Dan Qiu Hongjie Li Tongde Bie School of Food and biological engineering Jiangsu University Zhenjiang Jiangsu 212013 China School of Environment Jiangsu University Zhenjiang Jiangsu 2120131 China key laboratory of Wheat Biology and genetic Improvement on Low & Middle Yangtze River Valley Wheat Region (Ministry of Agriculture) Yangzhou Academy of Agricultural Sciences Yangzhou Jiangsu 225007 China National key Facility for Crop gene resources and genetic Improvement (NFCRI) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Dear Editor, Common wheat （Triticum aestivum, 2n = 6x = 42, AABBDD） is one of the most widely cultivated cereal crops, providing -20% of the calories consumed by humans （Fu et al., 2009）. However, wheat production is constantly challenged by powdery mildew disease, which occurs globally and is caused by the biotrophic fungal pathogen Blumena graminis f. sp. tritici （Bgt）. Utilization of powdery mildew resistance （Pm） genes is the most effective and economical way to control powdery mildew disease.

关键词： Pm21 疾病蛋白质小麦粉编码用途普通小麦小麦生产

Maternal miR-202-5p is required for zebrafish primordial germ cell migration by protecting small GTPase Cdc42

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Journal of Molecular Cell Biology 2020年第7期12卷 530-542页

作者： Yilin Jin Wei Liu Yangxi Xiang Wanwan Zhang Hong Zhang Kuntong Jia Meisheng Yi School of Marine Sciences Sun Yat-sen University Guangzhou 519082China Guangdong Provincial key laboratory of Marine resources and Coastal engineering Sun Yat-sen UniversityGuangzhou 519082China Southern Marine Science and engineering Guangdong laboratory(Zhuhai) Sun Yat-sen UniversityGuangzhou 519082China

In many lower animals,germ cell formation,migration,and maintenance depend on maternally provided determinants in germ *** zebrafish,these processes have been extensively studied in terms of RNA-binding proteins and other coding *** role of small non-coding RNAs in the regulation of primordial germ cell(PGC)development remains largely unknown and poorly investigated,even though growing interests for the importance of miRNAs involved in a wide variety of biological ***,we reported the role and mechanism of the germ plasm-specific miRNA miR-202-5p in PGC migration:(i)both maternal loss and knockdown of miR-202-5p impaired PGC migration indicated by the mislocalization and reduced number of PGCs;(ii)cdc42se1 was a direct target gene of miR-202-5p,and overexpression of Cdc42se1 in PGCs caused PGC migration defects similar to those observed in loss of miR-202-5p mutants;(iii)Cdc42se1 not only interacted with Cdc42 but also inhibited cdc42 transcription,and overexpression of Cdc42 could rescue PGC migration defects in Cdc42se1 overexpressed ***,miR-202-5p regulates PGC migration by directly targeting and repressing Cdc42se1 to protect the expression of Cdc42,which interacts with actin to direct PGC migration.

关键词： primordial germ cell miR-202-5p Cdc42se1 Cdc42 migration

Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in ***

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Synthetic and Systems Biotechnology 2021年第4期6卷 326-334页

作者： Zehua Chen Jinyuan Sun Ying Guan Ming Li Chunbo Lou Bian Wu CAS key laboratory of Microbial Physiological&Metabolic engineering and State key laboratory of Microbial resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China College of Life Sciences University of Chinese Academy of SciencesBeijing100149China Tsinghua University Beijing100084China

The development of base editing(BE)technology has opened a new avenue for research studies in bacteriology,particularly for bacterial species in which the DNA double-strand breaks(DSBs)introduced by CRISPR/Cas system would lead to cell ***,a major limitation of BE-mediated gene editing is the restricted editable sites in the target bacterial genome due to highly diverse genomic compositions,such as GC ***,we developed a broad-spectrum DNase-inactive Cpf1(dCpf1)variant from Francisella novicida(bsdFnCpf1)through directed *** resulting optimized mutant showed a substantially expanded targeting range,including previously non-canonical protospacer-adjacent motifs(PAMs),especially the GC-rich *** deaminase APOBEC1 and uracil DNA glycosylase inhibitor(UGI)were fused with bsdFnCpf1 to achieve specific C to T mutations at multiple target sites with canonical or non-canonical PAMs in the *** genome without compromising cell *** anticipate that bsdFnCpf1 could be applied for multiplex gene regulation and BE in species that have been reported to be suitable for Cpf1.

关键词： Base editing Directed evolution Broad-spectrum dFnCpf1(bsdFnCpf1) Multiple gene editing

The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis via blocking inactive rhomboid protein 2-dependent pathway

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Acta Pharmaceutica Sinica B 2023年第3期13卷 1071-1092页

作者： Minxuan Xu Jun Tan Liancai Zhu Chenxu Ge Wei Dong Xianling Dai Qin Kuang Shaoyu Zhong Lili Lai Chao Yi Qiang Li Deshuai Lou Linfeng Hu Xi Liu Gang Kuang Jing Luo Jing Feng Bochu Wang Chongqing key laboratory of Medicinal resources in the Three Gorges Reservoir Region School of Biological and Chemical EngineeringChongqing University of EducationChongqing 400067China College of Modern Health Industry Chongqing University of EducationChongqing 400067China key laboratory of Biorheological Science and Technology(Chongqing University) Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400030China Shandong Cancer Hospital and Institute Shandong First Medical University&Shandong Academy of Medical SciencesJinan 250117China

Nowadays potential preclinical drugs for the treatment of nonalcoholic steatohepatitis(NASH)have failed to achieve expected therapeutic efficacy because the pathogenic mechanisms are *** rhomboid protein 2(IRHOM2),a promising target for treatment of inflammation-related diseases,contributes to deregulated hepatocyte metabolism-associated nonalcoholic steatohepatitis(NASH)***,the molecular mechanism underlying Irhom2 regulation is still not completely *** this work,we identify the ubiquitin-specific protease 13(USP13)as a critical and novel endogenous blocker of IRHOM2,and we also indicate that USP13 is an IRHOM2-interacting protein that catalyzes deubiquitination of Irhom2 in ***-specific loss of the Usp13 disrupts liver metabolic homeostasis,followed by glycometabolic disorder,lipid deposition,increased inflammation,and markedly promotes NASH ***,transgenic mice with Usp13 overexpression,lentivirus(LV)-or adeno-associated virus(AAV)-driven Usp13 gene therapeutics mitigates NASH in 3 models of ***,in response to metabolic stresses,USP13 directly interacts with IRHOM2 and removes its K63-linked ubiquitination induced by ubiquitin-conjugating enzyme E2N(UBC13),a ubiquitin E2 conjugating enzyme,and thus prevents its activation of downstream cascade ***13 is a potential treatment target for NASH therapy by targeting the Irhom2 signaling pathway.

关键词： Usp13 Irhom2 NASH Hepatosteatosis Ubc13 NAFLD Ubiquitination Liver inflammation

Variation at the transcriptional level among Chinese natural populations of Arabidopsis thaliana in response to cold stress

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Chinese Science Bulletin 2008年第19期53卷 2989-2999页

作者： HE Fei KANG JiuQing ZHOU Xin SU Zhen QU LiJia GU HongYa National laboratory of Protein engineering and Plant genetic engineering Peking-Yale Joint Center for Plant Molecular Genetics and AgroBiotechnology College of Life Sciences Peking University Beijing 100871 China College of biological Sciences China Agriculture University Beijing 100094 China National Plant gene Research Center (Beijing) Beijing 100101 China

The Arabidopsis 25K geneChip (ATH1, Affymetrix) was used to make a survey of the variation of the transcriptional profiles among 5 Chinese natural populations of Arabidopsis thaliana under cold treatment. In normal growth condition, the expression level of 2.26% (513 genes in the population from Jiujiang, Jiangxi, JXjjx) to 6.52% (1482 genes in the population from Tongliang, Chongqing, CQtlx) genes was 2-fold higher than that of Col ecotype. Under cold treatment, the expression of 12.84% (2920 genes in the population from Chenggu, Shaanxi, SXcgx) to 19.46% (4426 genes in the population from Qinghe, xinjiang, XJqhx) genes was up- or down-regulated by at least two-fold that of their controls. In general, most of up-regulated genes might be the genes essential for plant surviving at low temperature, such as genes in CBF pathway and the genes responsible for synthesizing molecules accumulated for cold tolerance. However, each natural population had some specific genes induced under cold treatment. The data indicated that some of the cold-responding genes were differentiated among the populations distributed in the natural habitats with different climate conditions. CBF3, one of the key tran-scription factor genes in cold responding pathway, showed significant differences in expression among populations. The sequence analysis indicated that the changes in its regulation region caused the dramatic difference in the expression pattern. Further studies on the correlation of the function of the differentially expressed genes and the cold tolerance in different populations may provide some new insight into the molecular mechanism of adaptation to local environment in Arabidopsis thaliana in China.

关键词：中国人口调查基因转录实验研究