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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 788-794页

作者： Hong-Tao XIE Ming-Gang LEI Yuan-Zhu XIONG* Chang-Yan DENG Si-Wen JIANG Bo ZUO Feng-E LI De-Quan XU Tao WANG key laboratory of Swine genetics and breeding,Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China key laboratory of Swine genetics and breeding Ministry of Agriculture Huazhong Agricultural UniversityWuhan 430070 China

In order to detect the molecular basis of heterosis in pigs,suppression subtractivehybridization was carried out to investigate the difference in gene expression in the Longissimus dorsimuscle tissues between Meishan×Yorkshire F1 crossbreeds and their parents,Meishan *** swinemyosin regulatory light chain 2 (MRLC2) gene differentially expressed between the crossbreeds and thepurebreds was isolated and identified using semi-quantitative reverse transcriptase polymerase chain reactionand its complete cDNA sequence was obtained using the rapid amplification of cDNA ends *** sequence of the gene is not homologous to any of the known porcine *** sequenceprediction analysis reveals that the open reading frame of this gene encodes a protein of 172 amino acidscontaining the putative conserved domain of the EF-hand *** predicted amino acid sequenceof porcine MRLC2 protein exhibits 99%,98%,98%,98% and 97% identity with that of cattle,human,dog,rat and mouse,*** homology analysis revealed that the MRLC2 protein was very muchconserved in *** tissue expression analysis indicated that the swine MRLC2 gene is highlyexpressed in muscle,fat,heart,liver,spleen,lung,kidney,stomach,small intestine,ovary and testis,but notexpressed in pancreas.

关键词： MRLC2 gene pig heterosis Longissimus dorsi suppression subtractive hybridization rapid amplification of cDNA end

Identification of single nucleotide polymorphisms of PIK3R1 and DUSP1 genes and their genetic associations with milk production traits in dairy cows

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Journal of Animal Science and Biotechnology 2020年第1期11卷 9-19页

作者： Bo Han Yuwei Yuan Lijun Shi Yanhua Li Lin Liu Dongxiao Sun Department of Animal genetics Breeding and ReproductionCollege of Animal Science and TechnologyKey Laboratory of Animal GeneticsBreeding and Reproduction of Ministry of Agriculture and Rural AffairsNational Engineering Laboratory for Animal BreedingChina Agricultural UniversityNo.2 Yuanmingyuan West RoadHaidian DistrictBeijing 100193China Beijing Dairy cattle Center Beijing 100192China.

Background:Previously,phosphoinositide-3-kinase regulatory subunit 1(PIK3R1)and dual specificity phosphatase 1(DUSP1)were identified as promising candidate genes for milk production traits due to their being differentially expressed between the dry period and the peak of lactation in livers of dairy ***,in this study,the single nucleotide polymorphisms(SNPs)of PIK3R1 and DUSP1 genes were identified and their genetic associations with milk yield,fat yield,fat percentage,protein yield,and protein percentage,were investigated using 1067 Chinese Holstein cows from 40 sire ***:By re-sequencing the entire coding region and 2000 bp of the 5′and 3′flanking regions of the two genes,one SNP in the 5′untranslated region(UTR),three in the 3′UTR,and two in the 3′flanking region of PIK3R1 were identified,and one in the 5′flanking region,one in the 3′UTR,and two in the 3′flanking region of DUSP1 were *** single-locus association analyses showed that five SNPs in PIK3 R1,rs42590258,rs210389799,rs208819656,rs41255622,rs133655926,and rs211408208,and four SNPs in DUSP1,rs207593520,rs208460068,rs209154772,and rs210000760,were significantly associated with milk,fat and protein yields in the first or second lactation(P values≤0.0001 and 0.0461).In addition,by the Haploview 4.2 software,the six and four SNPs in PIK3R1 and DUSP1 respectively formed one haplotype block,and the haplotype-based association analyses showed significant associations between their haplotype combinations and the milk traits in both two lactations(P values≤0.0001 and 0.0364).One SNP,rs207593520(T/G),was predicted to alter the transcription factor binding sites(TFBSs)in the 5′flanking region of ***,the dual-luciferase assay showed that the transcription activity of allele T in rs207593520 was significantly higher than that of allele G,suggesting the activation of transcriptional activity of DUSP1 gene by allele T of ***,the rs207593520 SNP was highlighted as a

关键词： Chinese Holstein DUSP1 Genetic association Milk production PIK3R1 SNP

MED4 gene positively affects preadipocyte differentiation in Chinese red steppe cattle

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BIOCELL 2023年第9期47卷 2115-2123页

作者： MINGHONG WEI CHENG XIAO JIAN WU LIHONG QIN HONGLIANG LIU YANG CAO YUMIN ZHAO Institute of Animal Biotechnology Jilin Academy of Agricultural SciencesGongzhuling136100China Institute of Muscle Biology and Growth Research Institute for Farm Animal Biology(FBN)Dummerstorf18196Germany key laboratory of beef cattle genetics and breeding Ministry of Agriculture and Rural AffairsGongzhuling136100China

Background:The regulatory genes related to lipid metabolism affect the intramuscular fat(IMF)content and improve meat quality *** Complex Subunit 4(MED4),a vitamin D receptor protein,affects vitamin D,livestock growth,carcass traits,and triglyceride ***,the physiological function of the MED4 gene on bovine adipocyte differentiation remains ***:This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe *** overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western ***:The MED4 gene had significantly high expression during preadipocyte differentiation(pcattle by regulating the PPARγgene.

关键词： Chinese red steppe cattle MED4 PPARγ Predipocyte differentiation Lipid metabolism Meat quality trait

Establishment of paternity testing system using microsatellite markers in Chinese Holstein

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Journal of genetics and Genomics 2008年第5期35卷 279-284页

作者： Fei Tian Dongxiao Sun Yuan Zhang College of Animal Science and Technology and key laboratory of Animal genetics and breeding of Ministry of Agriculture China Agricultural University Beijing 100094 China

To estimate the efficiency of microsatellite markers in paternity testing among Chinese Holstein, 30 microsatellite loci were used to differentiate 330 Chinese Holstein genotypes, according to the calculation of the allele frequency, number of alleles, effective number of alleles, genetic heterozygosity, polymorphic information content （PIC）, and the exclusion probability in this cattle population. The results demonstrated that the exclusion probability ranged from 0.620 in locus BM1818 to 0.265 in locus INRA005 with the average of 0.472 and 11 microsatellite markers exceeding 0.5. The combined exclusion probability of nine microsatellite markers was over 0.99. The result showed that paternity testing of Chinese Holstein was basically resolved using the nine microsatellite markers selected.

关键词： Chinese Holstein rnicrosatellite paternity testing exclusion probability

Evaluation of breeding Programs Combining Genomic Information in Chinese Holstein

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Agricultural Sciences in China 2011年第12期10卷 1949-1957页

作者： CHEN Jun WANG Ya-chun ZHANG Yi SUN Dong-xiao ZHANG Sheng-li ZHANG Yuan College of Animal Science and Technology/National Engineering laboratory for Animal breeding/key laboratory of Agricultural Animal genetics and breeding China Agricultural University Beijing 100193 P.R.China

Current study adopted gene flow theory and selection index method to compare the breeding efficiency of three breeding plans in the Chinese Holstein cattle using ZPLAN software. Simulated conventional progeny-testing program （PT） and young sire program （YS） were compared with breeding program using genomic selection （GS） taking parameters derived from Chinese Holstein breeding system. The results showed that, GS shortened generation interval by 1.5-2.2 years, and increased the genetic progress by 30-50%, comparing to PT and YS, respectively. Economic analysis showed that GS could obtain a higher breeding efficiency, being 119 and 97% higher than that of PT and YS, respectively; and GS was also powerful in improving functional traits with a low heritability. Main factors affecting breeding efficiency in GS were further discussed, including selection intensity, accuracy and the cost of SNP genotyping. Our finding provided references for future designing and implementing GS in Chinese dairy population.

关键词： Chinese Holstein genomic selection progeny-testing program young sire program breeding plan

A novel method for rapid and reliable detection of complex vertebral malformation and bovine leukocyte adhesion deficiency in Holstein cattle

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Journal of Animal Science and Biotechnology 2012年第3期3卷 130-135页

作者： Yi Zhang Xuehua Fan Dongxiao Sun Yachun Wang Ying Yu Yan Xie Shengli Zhang Yuan Zhang National Engineering laboratory for Animal breeding Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of AgricultureCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background： Complex vertebral malformation （CVM） and bovine leukocyte adhesion deficiency （BLAD） are two autosomal recessive lethal genetic defects frequently occurring in Holstein cattle, identifiable by single nucleotide polymorphisms. The objective of this study is to develop a rapid and reliable genotyping assay to screen the active Holstein sires and determine the carrier frequency of CVM and BLAD in Chinese dairy cattle population. Results： We developed real-time PCR-based assays for discrimination of wild-type and defective alleles, so that carriers can be detected. Only one step was required after the DNA extraction from the sample and time consumption was about 2 hours. A total of 587 Chinese Holstein bulls were assayed, and fifty-six CVM-carriers and eight BLAD-carriers were identified, corresponding to heterozygote carrier frequencies of 9.54% and 1.36%, respectively. The pedigree analysis showed that most of the carriers could be traced back to the common ancestry, Osborndale Ivanhoe for BLAD and Pennstate Ivanhoe Star for CVM. Conclusions： These results demonstrate that real-time PCR is a simple, rapid and reliable assay for BLAD and CVM defective allele detection. The high frequency of the CVM allele suggests that implementing a routine testing system is necessary to gradually eradicate the deleterious gene from the Chinese Holstein population.

关键词： Holstein cattle BTA1 Leukocyte Adhesion Deficiency Defective Allele Lethal Recessive Allele

A post-GWAS confirming the genetic effects and functional polymorphisms of AGPAT3 gene on milk fatty acids in dairy cattle

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Journal of Animal Science and Biotechnology 2021年第1期12卷 122-140页

作者： Lijun Shi Xin Wu Yuze Yang Zhu Ma Xiaoqing Lv Lin Liu Yanhua Li Feng Zhao Bo Han Dongxiao Sun Department of Animal genetics Breeding and ReproductionCollege of Animal Science and TechnologyKey Laboratory of Animal GeneticsBreeding and Reproduction of Ministry of Agriculture and Rural AffairsNational Engineering Laboratory for Animal BreedingChina Agricultural UniversityNo.2 Yuanmingyuan West RoadHaidian DistrictBeijing 100193China Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China Beijing General Station of Animal Husbandry Beijing 100101China Beijing Dairy cattle Center Beijing 100192China.

Background: People are paying more attention to the healthy and balanced diet with the improvement of their living standards. Milk fatty acids(FAs) have been reported that they were related to some atherosclerosis and coronary heart diseases in human. In our previous genome-wide association study(GWAS) on milk FAs in dairy cattle, 83 genomewide significant single nucleotide polymorphisms(SNPs) were detected. Among them, two SNPs, ARS-BFGL-NGS-109493 and BTA-56389-no-rs associated with C18 index(P = 0.0459), were located in the upstream of 1-acylglycerol-3-phosphate O-acyltransferase 3(AGPAT3) gene. AGPAT3 is involved in glycerol-lipid, glycerol-phospholipid metabolism and phospholipase D signaling pathways. Hence, it was inferred as a candidate gene for milk FAs. The aim of this study was to further confirm the genetic effects of the AGPAT3 gene on milk FA traits in dairy ***: Through re-sequencing the complete coding region, and 3000 bp of 5′ and 3′ regulatory regions of the AGPA T3 gene, a total of 17 SNPs were identified, including four in 5′ regulatory region, one in 5′ untranslated region(UTR),three in introns, one in 3′ UTR, and eight in 3′ regulatory region. By the linkage disequilibrium(LD) analysis with Haploview4.1 software, two haplotype blocks were observed that were formed by four and 12 identified SNPs,respectively. Using SAS9.2, we performed single locus-based and haplotype-based association analysis on 24 milk FAs in 1065 Chinese Holstein cows, and discovered that all the SNPs and the haplotype blocks were significantly associated with C6:0, C8:0 and C10:0(P A, g.146704373 A > G, g.146704618 A > G and g.146704699 G > A) changed the transcription factor binding sites(TFBSs) for transcription factors SMARCA3, REX1, VMYB, BRACH, NKX26, ZBED4, SP1, USF1, ARNT and FOXA1. Out of them, two SNPs were validated to impact transcriptional ac

关键词： AGPAT3 Chinese Holstein Genetic effects Milk fatty acids Potential causal mutation

Genetic parameters for somatic cell score and production traits in the fi rst three lactations of Chinese Holstein cows

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Journal of Integrative Agriculture 2015年第1期14卷 125-130页

作者： ZHAO Fu-ping GUO Gang WANG Ya-chun GUO Xiang-yu ZHANG Yuan DU Li-xin National Center for Molecular genetics and breeding of Animal Institute of Animal SciencesChinese Academy of Agricultural Sciences Beijing Sanyuan Dairy cattle breeding Center College of Animal Science and Technology China Agricultural University

The objectives of this study were to estimate genetic parameters of lactation average somatic cell scores （LSCS） and examine genetic associations between LSCS and production traits in the first three lactations of Chinese Holstein cows using single-parity multi-trait animal model and multi-trait repeatability animal model. There were totally 273605 lactation records of Chinese Holstein cows with first calving from 2001 to 2012. Heritability estimates for LSCS ranged from 0.144 to 0.187. Genetic correlations between LSCS and 305 days milk, protein percentage and fat percentage were -0.079, -0.082 and -0.135, respectively. Phenotypic correlation between LSCS and 305 days milk yield was negative （-0.103 to -0.190）. Genetic correlation between 305 days milk and fat percentage or protein percentage was highly negative. Genetic correlation between milk fat percentage and milk protein percentage was highly favorable. Heritabilities of production traits decreased with increase of parity, whereas heritability of LSCS increased with increase of parity.

关键词： somatic cell score genetic parameters Chinese Holstein single-parity multi-trait animal model multi-traitrepeatability animal model

Active dry yeast supplementation improves the growth performance,rumen fermentation, and immune response of weaned beef calves

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Animal Nutrition 2021年第4期7卷 1352-1359页

作者： Jian Ma Cheng Wang Zhisheng Wang Guang Cao Rui Hu Xueying Wang Huawei Zou Kun Kang Quanhui Peng Bai Xue Lizhi Wang Yueming Zhu Xiaopeng Zhu Low Carbon breeding cattle and Safety Production University key laboratory of Sichuan Province Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130China Angel Yeast Co. Ltd.Yichang 443000China Zhangye Comprehensive Experimental Station of National Yak and beef Industrial Technology System Zhangye 734000China

The objective of this experiment was to investigate the potential benefits of active dry yeast(ADY)on the growth performance,rumen fermentation,nutrient digestibility,and serum parameters of weaned beef *** Simmental crossbred male calves(body weight=86.47±4.41 kg and 70±4 d of age)were randomly divided into 2 groups:control(CON)(fed basal ration)and ADY(fed basal ration and 5 g/d ADY per calf).The dietary concentrate-to-roughage ratio was 35:*** the calves were regularly provided rations 3 times a day at 07:00,13:00,and 19:00 and had free access to wate *** experiment lasted for 60 *** average daily gain of ADY group was higher(P=0.007)than that of the CON group,and the ratio of feed intake to average daily gain in the ADY group was reduced(P=0.022)as compared to the CON *** concentration of ruminal ammonia-N was higher(P=0.023)in the CON group than that in the ADY group,but an opposite trend of microbial protein was found between the 2 ***,the ruminal concentrations of propionate and butyrate were higher(Pbeef calves.

关键词： Active dry yeast Weaned beef calf Growth performance Rumen fermentation Nutrient digestibility Immune response

Statistical considerations for genomic selection

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Frontiers of Agricultural Science and Engineering 2017年第3期4卷 268-278页

作者： Huimin KANG Lei ZHOU Jianfeng LIU National Engineering laboratory for Animal breeding/key laboratory of Animal genetics Breeding and ReproductionMinistry of Agriculture/College of Animal Science and TechnologyChina Agricultural University

Genomic selection is becoming increasingly important in animal and plant breeding, and is attracting greater attention for human disease risk prediction. This review covers the most commonly used statistical methods and some extensions of them, i.e., ridge regression and genomic best linear unbiased prediction, Bayesian alphabet, and least absolute shrinkage and selection *** it discusses the measurement of the performance of genomic selection and factors affecting the prediction of performance. Among the measurements of prediction performance, the most important and commonly used measurement is prediction accuracy. In simulation studies where true breeding values are available, accuracy of genomic estimated breeding value can be calculated directly. In real or industrial data studies, either trainingtesting approach or k-fold cross-validation is commonly employed to validate methods. Factors influencing the accuracy of genomic selection include linkage disequilibrium between markers and quantitative trait loci, genetic architecture of the trait, and size and composition of the training population. Genomic selection has been implemented in the breeding programs of dairy cattle, beef cattle, pigs and poultry. Genomic selection in other species has also been intensively researched, and is likely to be implemented in the near future.

关键词： genomic estimated breeding value genomic selection linkage disequilibrium statistical methods